Currently,irrigation decisions in coffee cultivation primarily rely on empirical knowledge,resulting in inefficient practices.Combining real-time leaf water content(LWC)data can help improve the accuracy of the irriga...Currently,irrigation decisions in coffee cultivation primarily rely on empirical knowledge,resulting in inefficient practices.Combining real-time leaf water content(LWC)data can help improve the accuracy of the irrigation planning.Spectral remote sensing is a fast,reliable,and non-invasive method to detect vegetation moisture content.In this study,a model to estimate the LWC of Coffea arabica L.was built using hyperspectral reflectance of the canopy under various irrigation levels.For this purpose,common spectral indices,two-band spectral indices[ratio spectral index(RSI);difference spectral index(DSI);and normalized difference spectral index(NDSI)],and three-band spectral indices were constructed.Feature bands were extracted using the successive projections algorithm(SPA).Optimal spectral indices were extracted using the correlation coefficient method,and the feature wavebands and spectral indices were combined into five datasets.These datasets were split into modeling and validation datasets by sample set partitioning based on the joint x-y distance(SPXY)algorithm.A linear model[partial least squares regression(PLSR)]and three non-linear models[support vector machine(SVM);extreme learning machine(ELM);back propagation artificial neural network(BPANN)]were built to estimate LWC of Coffea arabica L.The results indicated that the non-linear models surpassed the linear model.The accuracy was the highest when the modeling was performed using the dataset combination 5.Among various modeling methods,the predictive performance of ELM was the best(modeling dataset:R^(2)=0.745,RMSE=2.241%,RRMSE=3.482%;validation dataset:R^(2)=0.721,RMSE=2.142%,RRMSE=3.364%).ELM outperformed PLSR,SVM,and BPANN in LWC retrieval.The obtained results indicated that the dataset built by the combined use of different methods was superior to those from a single data source in accuracy.This study provides a scientific basis for the quantitative diagnosis of coffee tree water status,with significant implications for optimizing field irrigation management.展开更多
Low dose remedies are widely administered in medicine. We used Tele-Stereo-EEG and the hippocampal slice preparation to measure physiological effects of orally given Coffea D6 (40 mg/kg), Gelsemium D4 (10 mg/kg) and V...Low dose remedies are widely administered in medicine. We used Tele-Stereo-EEG and the hippocampal slice preparation to measure physiological effects of orally given Coffea D6 (40 mg/kg), Gelsemium D4 (10 mg/kg) and Veratrum D6 (30 mg/kg) in rats. Adult rats were implanted with electrodes positioned stereotactically into four brain regions. Changes in field potentials were transmitted wirelessly. After frequency analysis data from 6 - 8 animals were averaged. For in vitro testing, preparations were superfused directly on hippocampal slices. Stimulation of Schaffer Collaterals by single stimuli (SS) or theta burst stimulation (TBS) resulted in stable population spike amplitudes. All three low dose preparations produced decreases of spectral power. Statistically significant changes were observed in delta, theta and alpha2 spectral power. In the hippocampal slice preparation Coffea facilitated signal transfer presumably by enhancing glutamate AMPA receptor transmission. Gelsemium showed a similar effect, but only after single shock stimulation. Opposite to this, attenuation of the electric pathway was recognized after theta burst stimulation due to AMPA receptor and glutamate metabotropic II receptor mediated transmission. Veratrum was able to attenuate glutamatergic due to receptor-mediated signalling sensitive to AMPA and NMDA. The results strongly speak in favour of the existence of biologically active molecules in these low dose preparations.展开更多
In the present research,the rational coupling mode of irrigation and shading cultivation for rapid growth and water saving of young Arabica coffee shrubs was investigated from 2016 to 2017.Taking full irrigation(FI,1....In the present research,the rational coupling mode of irrigation and shading cultivation for rapid growth and water saving of young Arabica coffee shrubs was investigated from 2016 to 2017.Taking full irrigation(FI,1.2Ep)as the control,the effects of three deficit irrigation(DI)(DI1,DI2 and DI3,with 1.0Ep,0.8Ep and 0.6Ep)on soil water content,temperature,microorganism population density,photosynthetic characteristics,canopy structure and dry mass of Arabica coffee under three shading cultivation modes(S0,monoculture coffee;S1,mild shading cultivation,intercropping with one line of Arabica coffee and one line of castor(Ricinus communis L.);S2,severe shading cultivation,intercropping with one line of Arabica coffee and two lines of castor)were investigated using plot experiments.Compared to FI,DI1 not obviously changed the population density of soil bacteria and actinomycetes,but increased net photosynthetic rate(Pn),crown area and dry mass of Arabica coffee by 7.0%,9.53%and 10.46%,respectively.In addition,DI1 also decreased total radiation under canopy(TRUC)by 5.51%.DI2 and DI3 reduced the population density of soil bacteria,fungi and actinomycetes ranging 8.94%-47.06%.Compared to S0,S1 increased the population density of soil fungi,bacteria and actinomycetes by 13.99%,30.77%and 9.72%,respectively.S1 also increased Pn,transpiration rate(Tr),leaf apparent radiation use efficiency(ARUE),leaf area index(LAI)and dry mass by 9.29%,5.39%,60.98%,10.31%and 30.02%,respectively.DI1S1 obtained the highest Pn and dry mass and higher LAI and the lowest TRUC.DI1S1 increased Pn,ARUE and dry mass by 18.98%,72.37%and 62.90%respectively but decreased TRUC by 21.77%when compared to FIS0.Thus,DI1S1 was found to be the rational mode of irrigation and shading cultivation for young Arabica coffee.展开更多
This study was aimed at establishing allometric models for estimating LA (Leaf Area) of eight Coffea arabica genotypes in Mana district of Jimma Zone Oromia Regional State, South Western Ethiopia (7°46'N, ...This study was aimed at establishing allometric models for estimating LA (Leaf Area) of eight Coffea arabica genotypes in Mana district of Jimma Zone Oromia Regional State, South Western Ethiopia (7°46'N, 36°0'E). Many Methodologies and instruments have been devised to facilitate measurement of leaf area. However, these methods are destructive, laborious and expensive. For modeling leaf area, leaf width, leaf length and leaf area of 1200 leaves (50 leaves for each genotype) was measured for model calibration and the respective measurements on 960 leaves were used for model validation. Linear measurement was taken from leaves and branch diameters of eight genotypes of C. arabica, cultivated in field following a randomized complete blocks design at three altitudes (High, Medium and Low) were evaluated to identify best option for input in the models, and to validate the method to estimate the leaf area. Linear and non-linear models were tested for their accuracy to predict leaf area of the eight C. arabica genotypes. The use of linear model resulted in high accuracy for all of the eight C. arabica genotypes. No significant effect of growing altitude and genotype was obtained among the slopes of the models. Therefore, one single model was fitted to the combined data of all genotypes at all altitudes (LA = 0.6434LW). Comparison between observed and predicted leaf area was made using this model in another independent dataset, conducted for model validation, exhibited a high degree of correlation (r = 0.98 - 0.99, P < 0. 01). The over or under estimation of the leaf area using this model ranges between 0.02% to 1.7% and this model is adequate to estimate the leaf area for the eight C. arabica genotypes. Hence, this model can be proposed to be reliably used and with this developed model, researchers can estimate the leaf area of newly released eight genotypes of C. arabica at different altitudes accurately.展开更多
In principle, the free fatty acids, diterpene fatty acid esters and triglycerides from green coffee oil can be separated effectively, by a suitable separation process, due to the differences between molar mass and vap...In principle, the free fatty acids, diterpene fatty acid esters and triglycerides from green coffee oil can be separated effectively, by a suitable separation process, due to the differences between molar mass and vapor pressures. In addition, in the case of component separation by molecular distillation, boiling point is replaced by evaporation rate at a given temperature. Several experiments and theoretical analyses have been carried out to identify the impact of important parameters （mean free path, evaporation rate, relative volatility and Knudsen number）, which determine the performance of these processes and degree of separation. In this work, a process development based on molecular distillation, for the enrichment of coffee diterpenes from green coffee oil is presented. The distillates were enriched in diterpene fatty acid esters and free fatty acids, while the residues were enriched in high molar mass triglycerides.展开更多
Brazil is the largest coffee exporter in the world. In addition, it occupies the second position, among the consuming countries of the drink. To investigate the chemical composition and quality of the coffee drink fro...Brazil is the largest coffee exporter in the world. In addition, it occupies the second position, among the consuming countries of the drink. To investigate the chemical composition and quality of the coffee drink from Coffea arabica species, samples grown in the city of Ourinhos, the third most productive region in the state of Sao Paulo, a study of its properties and characteristics was conducted. The pharmacobotanical characteristics were investigated performed according to usual techniques in these researches through macroscopic and microscopic studies through cross-sections. The oil obtained for analysis was extracted by soxhlet, and the caffeine content was measured for green grains using High Performance Liquid Chromatography (HPLC) using as stationary phase, column C18 and gradient mobile phase formed by 80% water and 20% methanol. The concentration obtained was 44.983 ± 0.86 μg/mL. The antioxidant activity was measured in triplicate through the DPPH test of the in natura coffee oil, and presented antioxidant action of EC50 25.89 ± 1.16 μg/mL.展开更多
[Objective] Through the investigation of weeds in Coffea arabica orchads in NuJiang River basin, this study aimed to provide scientific guidance for the weed control and improving the ecological and economic benefits ...[Objective] Through the investigation of weeds in Coffea arabica orchads in NuJiang River basin, this study aimed to provide scientific guidance for the weed control and improving the ecological and economic benefits of the plantation of Cof- fea arabica. [Method] The types of weeds and the characteristics of weed occur- rence in Coffea arabica orchads in Nujiang River basin were investigated from July to August in 2012. [Result] The results showed that there were 69 types of weeds belonging to 21 families in Coffea arabica orchads. The predominant harmful types were shown as follows： Commelina nudiflora L. ＋ Leptochloa chinensis, Cyperus ro- tundus L. ＋ Bidens pilosa L. ＋ Eleusine indica, Imperata cylindrical ＋ Ageratum conyzoides L. ＋ Eupatorium odoratum L., Ageratum conyzoides L. ＋ Digitaria san- guinalis （L.） Scop. The preponderant weeds consisted mainly of 10 species, namely, Cyperus rotundus L., Commelina nudiflora L., Leptochloa chinensis, Digitaria san- guinalis （L.） Scop, Imperata cylindrical, Bidens pilosa L., Ageratum conyzoides L, E- upatorium odoratum L., Eleusine indica and Chenopodium serotinum L. [Conclusion] The result from this study is of great significance for the plantation of Coffea arabica in Nujiang River basin, as well as the control of weeds.展开更多
In present study,four new dammarane-type triterpenoids,namely caffruones A–D(1–4),were isolated from the cherries of Coffea arabica.Their structures were elucidated by extensive spectroscopic analysis including 1D,2...In present study,four new dammarane-type triterpenoids,namely caffruones A–D(1–4),were isolated from the cherries of Coffea arabica.Their structures were elucidated by extensive spectroscopic analysis including 1D,2D NMR(HSQC,HMBC,^(1)H–^(1)H COSY,and ROESY),HRMS and IR spectra.This is the first time that tetracyclic triterpenes have been reported in genus Coffea.展开更多
Coffee (Coffea arabica) is an economically important crop cultivated on the terraced slopes and narrow valleys of the mountains in the south-western Saudi Arabia. The objective of the study was to evaluate genetic div...Coffee (Coffea arabica) is an economically important crop cultivated on the terraced slopes and narrow valleys of the mountains in the south-western Saudi Arabia. The objective of the study was to evaluate genetic diversity among nineteen coffee accessions collected from different coffee growing areas based on quantitative morphological and pomological traits. Data of 17 quantitative traits were collected and subjected to various statistical analyses. The analysis of variance showed significant differences (P < 0.05) among the accessions for the majority of quantitative traits measured. A principal component analysis (PCA) and hierarchical cluster analysis (HCA) were used to determine the nature and degree of divergence among accessions. PCA showed that four principal components explained about 85.62% of the total variation. Differentiation of germplasm into different clusters was because of cumulative effects of a number of characters. Cluster and distance analysis of quantitative traits revealed the existence of five different groups. The maximum distance was seen between clusters IV and V (76.4) while the minimum was seen between I and II (16.13). Accessions like KSA6 and KSA8 from Tallen valley and KSA10 and KSA11 from Hada and Shada valleys collection are recommenced for the next breeding work as they are high yielder accessions compared to the others. However, it is suggested to complete the present results by additional assessment of the in situ collection and variety trials. These results are of great interest for future breeding programs for this species.展开更多
Five new ent-kaurane diterpenoids,named mascaroside Ⅲ–Ⅴ(1–3),and 20-nor-cofaryloside Ⅰ–Ⅱ(4–5),together with seven known diterpenoids,were isolated from methanol extracts of the green coffee beans of Yunnan Ara...Five new ent-kaurane diterpenoids,named mascaroside Ⅲ–Ⅴ(1–3),and 20-nor-cofaryloside Ⅰ–Ⅱ(4–5),together with seven known diterpenoids,were isolated from methanol extracts of the green coffee beans of Yunnan Arabica Coffee.Their chemical structures were elucidated by extensive spectroscopic analyses.Meanwhile,cytotoxicity assay against HL-60,A-549,SMMC-7721,MCF-7 and SW480 cell lines showed that they have not evident inhibition of cytotoxicity.展开更多
The aim of the study was to describe the drying kinetics of washed coffee (Coffea arabica L.) and evaluate the best mathematical model to fit the experimental drying data conducted with different air humidity (40%, 50...The aim of the study was to describe the drying kinetics of washed coffee (Coffea arabica L.) and evaluate the best mathematical model to fit the experimental drying data conducted with different air humidity (40%, 50% and 60%), temperatures (23, 40 and 60 °C) and the quality of the coffee. The cherries coffee were separated and standardized in the processes of washing, mechanical and manual separation. Then, approx. 85 kg of coffee cherries were pulped and taken directly to the yard. The washed coffee was completed dried in a mechanical dryer and yard. The results showed that the different conditions of the ambient air significantly influenced the processes of drying. The water content of the hygroscopic equilibrium of pulped coffee is directly proportional to the water activity and relative humidity (RH), decreasing with increasing temperature, for the same value of equilibrium. The Oswin model was best represented by the hygroscopicity of the pulped coffee, while the Midilli model shows the best fit to describe the drying curves of the washed coffee. The effective diffusion coefficient increases with increasing temperature of the drying air and reducing of RH, being described by the Arrhenius equation. Electrical conductivity, potassium leaching, total titratable acidity and grease acidity increase with increasing drying temperature regardless of the type of processing. Reducing sugars, total sugars and the sensorial quality was negatively affected with increasing drying temperature regardless of the type of processing. The drying at 60 °C/40% RH negatively affected the coffee quality.展开更多
Coffee Leaf Rust(CLR)is caused by Hemileia vastatrix in Coffea spp.It is one of the most dangerous phytopathogens for coffee plantations in terms of coffee productivity and coffee cup quality.In this review,we resume ...Coffee Leaf Rust(CLR)is caused by Hemileia vastatrix in Coffea spp.It is one of the most dangerous phytopathogens for coffee plantations in terms of coffee productivity and coffee cup quality.In this review,we resume the problem of CLR in Mexico and the pathogenesis of H.vastatrix.The review abord plant-pathogen interactions which lead a compatible or incompatible interactions and result in CLR disease or resistance,respectively.The review abord Coffea spp.defense response pathways involved in H.vastatrix pathogenicity.Additionally,current measures to control H.vastatrix proliferation and germination were aborded focused on phytosanitary actions,and biological and chemical control.Finally,new trendlines to reduce the impact of CLR as nanoparticles and nanotechnology were analyzed.展开更多
Mites belonging to the family Phytoseiidae are the most important and most widely studied among predatory mites of phytophagous mites. The phytophagous mites Brevipalpus phoenicis (Geijskes, 1939) (Tenuipalpidae) and ...Mites belonging to the family Phytoseiidae are the most important and most widely studied among predatory mites of phytophagous mites. The phytophagous mites Brevipalpus phoenicis (Geijskes, 1939) (Tenuipalpidae) and Oligonychus ilicis (McGregor, 1917) (Tetranychidae) on coffee (Coffea spp.), are frequently found in combination with the predaceous mites Iphiseiodes zuluagai Denmark & Muma, 1972;Euseius alatus DeLeon, 1966 and Amblyseius herbicolus (Chant, 1959) (Acari: Phytoseiidae), among others. The purpose of this research was to study the effects of the product CyazypyrTM (cyantraniliprole 100 OD) on these three species of Phytoseiidae, relevant to coffee, citrus and other agricultural crops in Brazil, following standard laboratory procedures. Mated female mites were exposed to fresh-dried residues on a glass surface, with 8 treatments, 5 mites per glass plate and 6 replicates, in a completely randomized experimental design. Each test lasted eight days, with a daily count of the surviving females and of eggs laid. CyazypyrTM, in all tested doses (75, 100, 125, 150, 175 and 200 g a.i./ha), was selective for the studied species, A. herbicolus, I. zuluagai, and E. alatus. Overall the treatments resulted in low mortality rates and negligible impact on the reproduction. Therefore, based on IOBC standards, CyazypyrTMcan be classified as not harmful (class 1) or slightly harmful (class 2), comparable to the agrochemical TalentoTM (hexythiazox 500 WP-12 g a.i./ha) equivalent to a harmless standard of selectivity in the laboratory. CyazypyrTM is therefore a complement to programs of integrated pest management, to preserve the populations of predatory mites in crops of coffee and citrus, among others, in Brazil.展开更多
We investigated the effects of formulations based on phosphite products on gas exchange and activity of antioxidant enzymes in coffee plants. Seedlings of the Mundo Novo cultivar were submitted to various treatments c...We investigated the effects of formulations based on phosphite products on gas exchange and activity of antioxidant enzymes in coffee plants. Seedlings of the Mundo Novo cultivar were submitted to various treatments composed of different formulations of with potassium phosphite (ADB 120), manganese phosphite (Reforce Mn), and fungicide (PrioriXtra®). For coffee seedlings, the combination of potassium phosphite and citrus by-products, isolated or in a combination with other products elicited the antioxidant system. Besides the high activity of antioxidant enzymes, the photosynthetic rates were higher than other treatments. The better performance of coffee seedlings treated with those formulations occurred even in absence of pathogens.展开更多
This study aimed to agronomically and morphologically characterize, as well as analyze the genetic divergence of Dilla ＆ Alghe x Timor hybrid progenies of the coffee germplasm bank of Minas Gerais in Brazil to improv...This study aimed to agronomically and morphologically characterize, as well as analyze the genetic divergence of Dilla ＆ Alghe x Timor hybrid progenies of the coffee germplasm bank of Minas Gerais in Brazil to improve the breeding and management schemes of the hybrids. Forty-two morpho-agronomic traits were evaluated and the generalized Mahalanobis distance was used to quantify the genetic divergence among progenies. Tocher clustering, unweighted pair-group method using arithmetic averages （UPGMA） method and canonical variation analysis were employed as clustering strategies. The Tocher and UPGMA methods have been separated the progenies into eight and five groups, respectively. Analysis of the relative contribution of each characteristic to genetic dissimilarity highlighted the characteristics of production in liters in 2009, production notes in 2009, and average uniformity of maturation in 2009 as those that contributed most to obtaining genetic divergence. The authors observed that the first three canonical variables accounted for over 99% of the total variance observed in the full set of analyzed characteristics. High variability was observed among the 15 progenies of Dilla ＆ Alghe × Timor hybrid progenies. The progenies MG0589 and MG0592, MG0593 and MG0589, MG0591 and MG0589, MG0589 and MG0588, MG0589 and MG0583 were the most suitable crosses for exploring the genetic variability in Dilla ＆ Alghe × Timor hybrid generated from the germplasm bank of Minas Gerais. Through these crossovers, it is expected to get new coffee cultivars with desirable agronomic characteristics, as resistant to Pseudomonas xyringae pv. garcae and some breeds ofHemileia vastatrix.展开更多
[Objectives]The paper was to elucidate the symptoms,morphological characteristics,etiological factors,and current extent of damage associated with coffee root rot disease.[Methods]The symptoms,morphological characteri...[Objectives]The paper was to elucidate the symptoms,morphological characteristics,etiological factors,and current extent of damage associated with coffee root rot disease.[Methods]The symptoms,morphological characteristics,and etiological factors associated with coffee root rot disease were systematically observed,described,and analyzed.The assessment of damage was carried out using a specialized investigative methodology.[Results]The application of bottom fertilizer containing Fusarium incarnatum facilitated the pathogen's entry through root wounds during transplantation,resulting in the development of water-soaked depression lesions in the affected areas.This infection significantly reduced the number of lateral roots in coffee plants,leading to symptoms such as wilting,withering,and ultimately,the death of the aboveground foliage.F.incarnatum exhibited three distinct types of spore morphology:macroconidia,which were sickle-shaped;mesoconidia,which were spindle-shaped;and microconidia,which were oval-shaped.The incidence rate of the disease in the affected region reached 100%,with a disease index exceeding 91,indicating severe damage.[Conclusions]This study serves as a valuable reference for the prevention and management of the emerging disease known as coffee root rot.展开更多
基金supported by the National Natural Science Foundation of China(Grant No.52379040)Yunnan Fundamental Research Projects(Grant No.202301AS070030 and 202101AU070039)+2 种基金Yunnan Major Science and Technology Special Plan(Grant No.202302AE090024)Yunnan Science and Technology Talent and Platform Program(Grant No.202305AM070006)the Yunnan Intelligent Water-Fertilizer-Pesticide Integration Technology and Equipment Innovation Team(No.202505AS350025).
文摘Currently,irrigation decisions in coffee cultivation primarily rely on empirical knowledge,resulting in inefficient practices.Combining real-time leaf water content(LWC)data can help improve the accuracy of the irrigation planning.Spectral remote sensing is a fast,reliable,and non-invasive method to detect vegetation moisture content.In this study,a model to estimate the LWC of Coffea arabica L.was built using hyperspectral reflectance of the canopy under various irrigation levels.For this purpose,common spectral indices,two-band spectral indices[ratio spectral index(RSI);difference spectral index(DSI);and normalized difference spectral index(NDSI)],and three-band spectral indices were constructed.Feature bands were extracted using the successive projections algorithm(SPA).Optimal spectral indices were extracted using the correlation coefficient method,and the feature wavebands and spectral indices were combined into five datasets.These datasets were split into modeling and validation datasets by sample set partitioning based on the joint x-y distance(SPXY)algorithm.A linear model[partial least squares regression(PLSR)]and three non-linear models[support vector machine(SVM);extreme learning machine(ELM);back propagation artificial neural network(BPANN)]were built to estimate LWC of Coffea arabica L.The results indicated that the non-linear models surpassed the linear model.The accuracy was the highest when the modeling was performed using the dataset combination 5.Among various modeling methods,the predictive performance of ELM was the best(modeling dataset:R^(2)=0.745,RMSE=2.241%,RRMSE=3.482%;validation dataset:R^(2)=0.721,RMSE=2.142%,RRMSE=3.364%).ELM outperformed PLSR,SVM,and BPANN in LWC retrieval.The obtained results indicated that the dataset built by the combined use of different methods was superior to those from a single data source in accuracy.This study provides a scientific basis for the quantitative diagnosis of coffee tree water status,with significant implications for optimizing field irrigation management.
文摘Low dose remedies are widely administered in medicine. We used Tele-Stereo-EEG and the hippocampal slice preparation to measure physiological effects of orally given Coffea D6 (40 mg/kg), Gelsemium D4 (10 mg/kg) and Veratrum D6 (30 mg/kg) in rats. Adult rats were implanted with electrodes positioned stereotactically into four brain regions. Changes in field potentials were transmitted wirelessly. After frequency analysis data from 6 - 8 animals were averaged. For in vitro testing, preparations were superfused directly on hippocampal slices. Stimulation of Schaffer Collaterals by single stimuli (SS) or theta burst stimulation (TBS) resulted in stable population spike amplitudes. All three low dose preparations produced decreases of spectral power. Statistically significant changes were observed in delta, theta and alpha2 spectral power. In the hippocampal slice preparation Coffea facilitated signal transfer presumably by enhancing glutamate AMPA receptor transmission. Gelsemium showed a similar effect, but only after single shock stimulation. Opposite to this, attenuation of the electric pathway was recognized after theta burst stimulation due to AMPA receptor and glutamate metabotropic II receptor mediated transmission. Veratrum was able to attenuate glutamatergic due to receptor-mediated signalling sensitive to AMPA and NMDA. The results strongly speak in favour of the existence of biologically active molecules in these low dose preparations.
基金supported by Chinese National Natural Science Fund(Grant No.51979133,51769010 and 51469010)KUST Analysis and Testing Fund(Grant No.2018T20090043).
文摘In the present research,the rational coupling mode of irrigation and shading cultivation for rapid growth and water saving of young Arabica coffee shrubs was investigated from 2016 to 2017.Taking full irrigation(FI,1.2Ep)as the control,the effects of three deficit irrigation(DI)(DI1,DI2 and DI3,with 1.0Ep,0.8Ep and 0.6Ep)on soil water content,temperature,microorganism population density,photosynthetic characteristics,canopy structure and dry mass of Arabica coffee under three shading cultivation modes(S0,monoculture coffee;S1,mild shading cultivation,intercropping with one line of Arabica coffee and one line of castor(Ricinus communis L.);S2,severe shading cultivation,intercropping with one line of Arabica coffee and two lines of castor)were investigated using plot experiments.Compared to FI,DI1 not obviously changed the population density of soil bacteria and actinomycetes,but increased net photosynthetic rate(Pn),crown area and dry mass of Arabica coffee by 7.0%,9.53%and 10.46%,respectively.In addition,DI1 also decreased total radiation under canopy(TRUC)by 5.51%.DI2 and DI3 reduced the population density of soil bacteria,fungi and actinomycetes ranging 8.94%-47.06%.Compared to S0,S1 increased the population density of soil fungi,bacteria and actinomycetes by 13.99%,30.77%and 9.72%,respectively.S1 also increased Pn,transpiration rate(Tr),leaf apparent radiation use efficiency(ARUE),leaf area index(LAI)and dry mass by 9.29%,5.39%,60.98%,10.31%and 30.02%,respectively.DI1S1 obtained the highest Pn and dry mass and higher LAI and the lowest TRUC.DI1S1 increased Pn,ARUE and dry mass by 18.98%,72.37%and 62.90%respectively but decreased TRUC by 21.77%when compared to FIS0.Thus,DI1S1 was found to be the rational mode of irrigation and shading cultivation for young Arabica coffee.
文摘This study was aimed at establishing allometric models for estimating LA (Leaf Area) of eight Coffea arabica genotypes in Mana district of Jimma Zone Oromia Regional State, South Western Ethiopia (7°46'N, 36°0'E). Many Methodologies and instruments have been devised to facilitate measurement of leaf area. However, these methods are destructive, laborious and expensive. For modeling leaf area, leaf width, leaf length and leaf area of 1200 leaves (50 leaves for each genotype) was measured for model calibration and the respective measurements on 960 leaves were used for model validation. Linear measurement was taken from leaves and branch diameters of eight genotypes of C. arabica, cultivated in field following a randomized complete blocks design at three altitudes (High, Medium and Low) were evaluated to identify best option for input in the models, and to validate the method to estimate the leaf area. Linear and non-linear models were tested for their accuracy to predict leaf area of the eight C. arabica genotypes. The use of linear model resulted in high accuracy for all of the eight C. arabica genotypes. No significant effect of growing altitude and genotype was obtained among the slopes of the models. Therefore, one single model was fitted to the combined data of all genotypes at all altitudes (LA = 0.6434LW). Comparison between observed and predicted leaf area was made using this model in another independent dataset, conducted for model validation, exhibited a high degree of correlation (r = 0.98 - 0.99, P < 0. 01). The over or under estimation of the leaf area using this model ranges between 0.02% to 1.7% and this model is adequate to estimate the leaf area for the eight C. arabica genotypes. Hence, this model can be proposed to be reliably used and with this developed model, researchers can estimate the leaf area of newly released eight genotypes of C. arabica at different altitudes accurately.
文摘In principle, the free fatty acids, diterpene fatty acid esters and triglycerides from green coffee oil can be separated effectively, by a suitable separation process, due to the differences between molar mass and vapor pressures. In addition, in the case of component separation by molecular distillation, boiling point is replaced by evaporation rate at a given temperature. Several experiments and theoretical analyses have been carried out to identify the impact of important parameters （mean free path, evaporation rate, relative volatility and Knudsen number）, which determine the performance of these processes and degree of separation. In this work, a process development based on molecular distillation, for the enrichment of coffee diterpenes from green coffee oil is presented. The distillates were enriched in diterpene fatty acid esters and free fatty acids, while the residues were enriched in high molar mass triglycerides.
文摘Brazil is the largest coffee exporter in the world. In addition, it occupies the second position, among the consuming countries of the drink. To investigate the chemical composition and quality of the coffee drink from Coffea arabica species, samples grown in the city of Ourinhos, the third most productive region in the state of Sao Paulo, a study of its properties and characteristics was conducted. The pharmacobotanical characteristics were investigated performed according to usual techniques in these researches through macroscopic and microscopic studies through cross-sections. The oil obtained for analysis was extracted by soxhlet, and the caffeine content was measured for green grains using High Performance Liquid Chromatography (HPLC) using as stationary phase, column C18 and gradient mobile phase formed by 80% water and 20% methanol. The concentration obtained was 44.983 ± 0.86 μg/mL. The antioxidant activity was measured in triplicate through the DPPH test of the in natura coffee oil, and presented antioxidant action of EC50 25.89 ± 1.16 μg/mL.
基金Supported by the Special Fund for Agro-scientific Research in the Public Interest,China(200903024-02)~~
文摘[Objective] Through the investigation of weeds in Coffea arabica orchads in NuJiang River basin, this study aimed to provide scientific guidance for the weed control and improving the ecological and economic benefits of the plantation of Cof- fea arabica. [Method] The types of weeds and the characteristics of weed occur- rence in Coffea arabica orchads in Nujiang River basin were investigated from July to August in 2012. [Result] The results showed that there were 69 types of weeds belonging to 21 families in Coffea arabica orchads. The predominant harmful types were shown as follows： Commelina nudiflora L. ＋ Leptochloa chinensis, Cyperus ro- tundus L. ＋ Bidens pilosa L. ＋ Eleusine indica, Imperata cylindrical ＋ Ageratum conyzoides L. ＋ Eupatorium odoratum L., Ageratum conyzoides L. ＋ Digitaria san- guinalis （L.） Scop. The preponderant weeds consisted mainly of 10 species, namely, Cyperus rotundus L., Commelina nudiflora L., Leptochloa chinensis, Digitaria san- guinalis （L.） Scop, Imperata cylindrical, Bidens pilosa L., Ageratum conyzoides L, E- upatorium odoratum L., Eleusine indica and Chenopodium serotinum L. [Conclusion] The result from this study is of great significance for the plantation of Coffea arabica in Nujiang River basin, as well as the control of weeds.
基金This study was supported financially by the National Natural Science Foundation of China,China(No.31670364)Project of Key New Productions of Yunnan Province,China(No.2015BB002)+2 种基金the STS Programme of Chinese Academy of Sciences,China(KFJ-SW-STS-143-8)Special Fund Project of Pu’er municipal government,China(2017)Foundation of State Key Laboratory of Phytochemistry and Plant Resources in West China,China(P2015-ZZ09).
文摘In present study,four new dammarane-type triterpenoids,namely caffruones A–D(1–4),were isolated from the cherries of Coffea arabica.Their structures were elucidated by extensive spectroscopic analysis including 1D,2D NMR(HSQC,HMBC,^(1)H–^(1)H COSY,and ROESY),HRMS and IR spectra.This is the first time that tetracyclic triterpenes have been reported in genus Coffea.
文摘Coffee (Coffea arabica) is an economically important crop cultivated on the terraced slopes and narrow valleys of the mountains in the south-western Saudi Arabia. The objective of the study was to evaluate genetic diversity among nineteen coffee accessions collected from different coffee growing areas based on quantitative morphological and pomological traits. Data of 17 quantitative traits were collected and subjected to various statistical analyses. The analysis of variance showed significant differences (P < 0.05) among the accessions for the majority of quantitative traits measured. A principal component analysis (PCA) and hierarchical cluster analysis (HCA) were used to determine the nature and degree of divergence among accessions. PCA showed that four principal components explained about 85.62% of the total variation. Differentiation of germplasm into different clusters was because of cumulative effects of a number of characters. Cluster and distance analysis of quantitative traits revealed the existence of five different groups. The maximum distance was seen between clusters IV and V (76.4) while the minimum was seen between I and II (16.13). Accessions like KSA6 and KSA8 from Tallen valley and KSA10 and KSA11 from Hada and Shada valleys collection are recommenced for the next breeding work as they are high yielder accessions compared to the others. However, it is suggested to complete the present results by additional assessment of the in situ collection and variety trials. These results are of great interest for future breeding programs for this species.
基金This work was supported financially by Programme of Key New Productions of Yunnan Province,Centre of CHINA(No.2015BB002)The STS Programme of Chinese Academy of Sciences(KFJ-SW-STS-143-8)as well as Foundation of State Key Laboratory of Phytochemistry and Plant Resources in West China(P2015-ZZ09).
文摘Five new ent-kaurane diterpenoids,named mascaroside Ⅲ–Ⅴ(1–3),and 20-nor-cofaryloside Ⅰ–Ⅱ(4–5),together with seven known diterpenoids,were isolated from methanol extracts of the green coffee beans of Yunnan Arabica Coffee.Their chemical structures were elucidated by extensive spectroscopic analyses.Meanwhile,cytotoxicity assay against HL-60,A-549,SMMC-7721,MCF-7 and SW480 cell lines showed that they have not evident inhibition of cytotoxicity.
文摘The aim of the study was to describe the drying kinetics of washed coffee (Coffea arabica L.) and evaluate the best mathematical model to fit the experimental drying data conducted with different air humidity (40%, 50% and 60%), temperatures (23, 40 and 60 °C) and the quality of the coffee. The cherries coffee were separated and standardized in the processes of washing, mechanical and manual separation. Then, approx. 85 kg of coffee cherries were pulped and taken directly to the yard. The washed coffee was completed dried in a mechanical dryer and yard. The results showed that the different conditions of the ambient air significantly influenced the processes of drying. The water content of the hygroscopic equilibrium of pulped coffee is directly proportional to the water activity and relative humidity (RH), decreasing with increasing temperature, for the same value of equilibrium. The Oswin model was best represented by the hygroscopicity of the pulped coffee, while the Midilli model shows the best fit to describe the drying curves of the washed coffee. The effective diffusion coefficient increases with increasing temperature of the drying air and reducing of RH, being described by the Arrhenius equation. Electrical conductivity, potassium leaching, total titratable acidity and grease acidity increase with increasing drying temperature regardless of the type of processing. Reducing sugars, total sugars and the sensorial quality was negatively affected with increasing drying temperature regardless of the type of processing. The drying at 60 °C/40% RH negatively affected the coffee quality.
文摘Coffee Leaf Rust(CLR)is caused by Hemileia vastatrix in Coffea spp.It is one of the most dangerous phytopathogens for coffee plantations in terms of coffee productivity and coffee cup quality.In this review,we resume the problem of CLR in Mexico and the pathogenesis of H.vastatrix.The review abord plant-pathogen interactions which lead a compatible or incompatible interactions and result in CLR disease or resistance,respectively.The review abord Coffea spp.defense response pathways involved in H.vastatrix pathogenicity.Additionally,current measures to control H.vastatrix proliferation and germination were aborded focused on phytosanitary actions,and biological and chemical control.Finally,new trendlines to reduce the impact of CLR as nanoparticles and nanotechnology were analyzed.
基金For DuPont do Brasil S.A.-Agricultura e Nutricao for the financial supportCNPq and INCT do CaféCNPq-Fapemig for the fellowship.
文摘Mites belonging to the family Phytoseiidae are the most important and most widely studied among predatory mites of phytophagous mites. The phytophagous mites Brevipalpus phoenicis (Geijskes, 1939) (Tenuipalpidae) and Oligonychus ilicis (McGregor, 1917) (Tetranychidae) on coffee (Coffea spp.), are frequently found in combination with the predaceous mites Iphiseiodes zuluagai Denmark & Muma, 1972;Euseius alatus DeLeon, 1966 and Amblyseius herbicolus (Chant, 1959) (Acari: Phytoseiidae), among others. The purpose of this research was to study the effects of the product CyazypyrTM (cyantraniliprole 100 OD) on these three species of Phytoseiidae, relevant to coffee, citrus and other agricultural crops in Brazil, following standard laboratory procedures. Mated female mites were exposed to fresh-dried residues on a glass surface, with 8 treatments, 5 mites per glass plate and 6 replicates, in a completely randomized experimental design. Each test lasted eight days, with a daily count of the surviving females and of eggs laid. CyazypyrTM, in all tested doses (75, 100, 125, 150, 175 and 200 g a.i./ha), was selective for the studied species, A. herbicolus, I. zuluagai, and E. alatus. Overall the treatments resulted in low mortality rates and negligible impact on the reproduction. Therefore, based on IOBC standards, CyazypyrTMcan be classified as not harmful (class 1) or slightly harmful (class 2), comparable to the agrochemical TalentoTM (hexythiazox 500 WP-12 g a.i./ha) equivalent to a harmless standard of selectivity in the laboratory. CyazypyrTM is therefore a complement to programs of integrated pest management, to preserve the populations of predatory mites in crops of coffee and citrus, among others, in Brazil.
文摘We investigated the effects of formulations based on phosphite products on gas exchange and activity of antioxidant enzymes in coffee plants. Seedlings of the Mundo Novo cultivar were submitted to various treatments composed of different formulations of with potassium phosphite (ADB 120), manganese phosphite (Reforce Mn), and fungicide (PrioriXtra®). For coffee seedlings, the combination of potassium phosphite and citrus by-products, isolated or in a combination with other products elicited the antioxidant system. Besides the high activity of antioxidant enzymes, the photosynthetic rates were higher than other treatments. The better performance of coffee seedlings treated with those formulations occurred even in absence of pathogens.
文摘This study aimed to agronomically and morphologically characterize, as well as analyze the genetic divergence of Dilla ＆ Alghe x Timor hybrid progenies of the coffee germplasm bank of Minas Gerais in Brazil to improve the breeding and management schemes of the hybrids. Forty-two morpho-agronomic traits were evaluated and the generalized Mahalanobis distance was used to quantify the genetic divergence among progenies. Tocher clustering, unweighted pair-group method using arithmetic averages （UPGMA） method and canonical variation analysis were employed as clustering strategies. The Tocher and UPGMA methods have been separated the progenies into eight and five groups, respectively. Analysis of the relative contribution of each characteristic to genetic dissimilarity highlighted the characteristics of production in liters in 2009, production notes in 2009, and average uniformity of maturation in 2009 as those that contributed most to obtaining genetic divergence. The authors observed that the first three canonical variables accounted for over 99% of the total variance observed in the full set of analyzed characteristics. High variability was observed among the 15 progenies of Dilla ＆ Alghe × Timor hybrid progenies. The progenies MG0589 and MG0592, MG0593 and MG0589, MG0591 and MG0589, MG0589 and MG0588, MG0589 and MG0583 were the most suitable crosses for exploring the genetic variability in Dilla ＆ Alghe × Timor hybrid generated from the germplasm bank of Minas Gerais. Through these crossovers, it is expected to get new coffee cultivars with desirable agronomic characteristics, as resistant to Pseudomonas xyringae pv. garcae and some breeds ofHemileia vastatrix.
基金Supported by Innovation Guidance and Technology-based Enterprise Cultivation Program of Yunnan Science and Technology Project(202304BP090027)Hu Faguang Expert Grassroots Scientific Research Workstation of Pu'er Aini Manor Coffee Co.,Ltd.
文摘[Objectives]The paper was to elucidate the symptoms,morphological characteristics,etiological factors,and current extent of damage associated with coffee root rot disease.[Methods]The symptoms,morphological characteristics,and etiological factors associated with coffee root rot disease were systematically observed,described,and analyzed.The assessment of damage was carried out using a specialized investigative methodology.[Results]The application of bottom fertilizer containing Fusarium incarnatum facilitated the pathogen's entry through root wounds during transplantation,resulting in the development of water-soaked depression lesions in the affected areas.This infection significantly reduced the number of lateral roots in coffee plants,leading to symptoms such as wilting,withering,and ultimately,the death of the aboveground foliage.F.incarnatum exhibited three distinct types of spore morphology:macroconidia,which were sickle-shaped;mesoconidia,which were spindle-shaped;and microconidia,which were oval-shaped.The incidence rate of the disease in the affected region reached 100%,with a disease index exceeding 91,indicating severe damage.[Conclusions]This study serves as a valuable reference for the prevention and management of the emerging disease known as coffee root rot.
