This review summarizes recent research progress on Poria cocos polysaccharide(PCP)in the areas of anticancer,anti-inflammatory,and antibacterial effects.It focuses on elucidating its mechanisms of action and highlight...This review summarizes recent research progress on Poria cocos polysaccharide(PCP)in the areas of anticancer,anti-inflammatory,and antibacterial effects.It focuses on elucidating its mechanisms of action and highlighting the current limitations in research.This aims to provide a reference for the further development and utilization of PCP.展开更多
用Copula函数刻画公司股价与核心一级资本比率(core tier 1 ratio,CTR)的相关性,然后通过模拟股价和CTR,建立了或有可转换债券(Co Co)以及带期权条款的或有可转换债券(Co Co Co)的定价模型.并将模型应用于塞浦路斯银行发行的CECS(conver...用Copula函数刻画公司股价与核心一级资本比率(core tier 1 ratio,CTR)的相关性,然后通过模拟股价和CTR,建立了或有可转换债券(Co Co)以及带期权条款的或有可转换债券(Co Co Co)的定价模型.并将模型应用于塞浦路斯银行发行的CECS(convertible enhanced capital securities)债券,发现用Copula刻画股价与CTR相关性的定价结果与债券实际价格的差异优于假设两者独立时的结果.最后结合国际上已发行的Co Co和Co Co Co债券的相关条款以及我国银监会对于减记债的基本要求,以交通银行为例设计了中国版的Co Co债券和Co Co Co债券,并依据所给模型对它们进行了数值计算.展开更多
Background Porcine epidemic diarrhea virus(PEDV)infection poses a significant challenge to the swine industry,with limited effective control measures available.Poria cocos polysaccharides(PCP)is the primary active ing...Background Porcine epidemic diarrhea virus(PEDV)infection poses a significant challenge to the swine industry,with limited effective control measures available.Poria cocos polysaccharides(PCP)is the primary active ingredient of Poria cocos,and has been demonstrated to show beneficial effects on intestinal damage in previous studies.However,its mechanism has not been fully understood.In the present study,18 seven-day-old piglets were divided into 3 groups:Control group,PEDV group,and PCP+PEDV group.After three days of adaptation,piglets in the PCP+PEDV group were orally administered 10 mg/kg body weight/d PCP from d 4 to 10.On d 8,piglets were orally administered with PEDV at the dose of 104.5 TCID50/piglet.This study aimed to investigate the potential effects of PCP on PEDVinduced intestinal injury and explored the underlying mechanisms.Results The results showed that PCP administration effectively alleviated diarrhea,reduced PEDV replication in the small intestine and colon of piglets,and significantly improved intestinal mucosal morphology.Specifically,PCP increased the villus height in both the jejunum and ileum and increased the villus height to crypt depth ratio in the ileum(P<0.05).Improved intestinal function was further evidenced by elevated plasma D-xylose levels and decreased diamine oxidase activity(P<0.05).Transcriptomic and proteomic analyses revealed that lipid metabolism is a key pathway regulated by PCP during PEDV infection.Notably,PCP significantly upregulated sphingolipid metabolism-related genes,including ectonucleotide pyrophosphatase/phosphodiesterase family member 7 and N-acylsphingosine amidohydrolase 2.Metabolomic analysis revealed that PCP primarily modulated the levels of plasmanylphosphoethanolamine,lysophosphatidylcholine,and carnitine.Additionally,PCP reversed the expression of key genes involved in fatty acid uptake,intracellular lipid transport,and fatty acid synthesis,such as fatty acid binding protein 2,fatty acid transport protein 4,apolipoprotein B,apolipoprotein C3,fatty acid synthase,long-chain fatty acyl CoA synthetase 3,lipoprotein lipase and acyl-CoA thioesterases 12(P<0.05).Conclusions These findings demonstrate that PCP mitigates PEDV-induced intestinal injury by modulating lipid metabolism and highlight its potential as a dietary supplement for enhancing anti-PEDV defenses and promoting intestinal health in piglets.展开更多
This study was conducted to extract pachyman to the greatest degree with ultrasonic technology, and to determine the content of pachyman by the phe-nol-sulfuric acid method, so as to choose the optimal technique of ul...This study was conducted to extract pachyman to the greatest degree with ultrasonic technology, and to determine the content of pachyman by the phe-nol-sulfuric acid method, so as to choose the optimal technique of ultrasonic extrac-tion of pachyman. Firstly, the ultrasonic power, extraction time and solid-to-liquid ra-tio were investigated by ultrasonic extraction method. Then, on this basis, a respon-sible surface experiment was designed, to select the optimal process conditions ac-cording to the index of pachyman yield. The optimized ultrasonic water extraction conditions were as fol ow： ultrasonic power of 100 W, extraction time of 30 min, and solid-to-liquid ratio at 1：40. The Oxford cup method was used to study the an-timicrobial activity of pachyman. lt was found that pachyman could significantly in-hibit Bacilus subtilis_and Staphylococcus aureus.展开更多
To investigate the gene expression profile of endosperm development, a cDNA library was constructed and characterized from the pulp of coconut at different developmental stages. The constructed cDNA library incorporat...To investigate the gene expression profile of endosperm development, a cDNA library was constructed and characterized from the pulp of coconut at different developmental stages. The constructed cDNA library incorporated approximately 1 × 10^7 clones in total, and the size of the insertion fragments ranged from 800 to 2 000 bp. Sequencing results of 100 randomly picked clones showed that the recombination rate was 96%. In subsequent sequence analysis, 41 clones （41%） were homologous to known function proteins, and 23 clones showed high amino acid identity （more than 80%） with the corresponding genes of different plants. Semi-quantitative RT-PCR indicated that oleosin and globulin genes are pulpspecific expression, and have differential expression level in different developmental stage. Clone 29, recognized as homologous to KIAA1239 protein （Homo sapiens）, was observed to occur nine times, indicating that this gene may be over-expressed during the endosperm development stage. However, the homologous protein was found only in mammals, and the detailed function is still unknown. Elucidation of the functional characterization of these genes will be carried out immediately.展开更多
基金Supported by Daqing Guiding Science and Technology Plan Project(zd-2025-047)2023"Three Vertical"Research Plan Project of Heilongjiang Bayi Agricultural University(ZRCPY202318)+1 种基金Central Government Supports Local College Reform and Development Fund Talent Training Project(2020GSP16)Heilongjiang Province Key Research and Development Plan Guidance Project(GZ20230021).
文摘This review summarizes recent research progress on Poria cocos polysaccharide(PCP)in the areas of anticancer,anti-inflammatory,and antibacterial effects.It focuses on elucidating its mechanisms of action and highlighting the current limitations in research.This aims to provide a reference for the further development and utilization of PCP.
文摘用Copula函数刻画公司股价与核心一级资本比率(core tier 1 ratio,CTR)的相关性,然后通过模拟股价和CTR,建立了或有可转换债券(Co Co)以及带期权条款的或有可转换债券(Co Co Co)的定价模型.并将模型应用于塞浦路斯银行发行的CECS(convertible enhanced capital securities)债券,发现用Copula刻画股价与CTR相关性的定价结果与债券实际价格的差异优于假设两者独立时的结果.最后结合国际上已发行的Co Co和Co Co Co债券的相关条款以及我国银监会对于减记债的基本要求,以交通银行为例设计了中国版的Co Co债券和Co Co Co债券,并依据所给模型对它们进行了数值计算.
基金supported by the National Natural Science Foundation of China(32172763,U22A20514)the National Key R&D Program of China(2022YFD130040302)+1 种基金the Hubei Provincial Key R&D Program(2023BBB040)the Hubei Important Science and Technology Project(2024BBA004).
文摘Background Porcine epidemic diarrhea virus(PEDV)infection poses a significant challenge to the swine industry,with limited effective control measures available.Poria cocos polysaccharides(PCP)is the primary active ingredient of Poria cocos,and has been demonstrated to show beneficial effects on intestinal damage in previous studies.However,its mechanism has not been fully understood.In the present study,18 seven-day-old piglets were divided into 3 groups:Control group,PEDV group,and PCP+PEDV group.After three days of adaptation,piglets in the PCP+PEDV group were orally administered 10 mg/kg body weight/d PCP from d 4 to 10.On d 8,piglets were orally administered with PEDV at the dose of 104.5 TCID50/piglet.This study aimed to investigate the potential effects of PCP on PEDVinduced intestinal injury and explored the underlying mechanisms.Results The results showed that PCP administration effectively alleviated diarrhea,reduced PEDV replication in the small intestine and colon of piglets,and significantly improved intestinal mucosal morphology.Specifically,PCP increased the villus height in both the jejunum and ileum and increased the villus height to crypt depth ratio in the ileum(P<0.05).Improved intestinal function was further evidenced by elevated plasma D-xylose levels and decreased diamine oxidase activity(P<0.05).Transcriptomic and proteomic analyses revealed that lipid metabolism is a key pathway regulated by PCP during PEDV infection.Notably,PCP significantly upregulated sphingolipid metabolism-related genes,including ectonucleotide pyrophosphatase/phosphodiesterase family member 7 and N-acylsphingosine amidohydrolase 2.Metabolomic analysis revealed that PCP primarily modulated the levels of plasmanylphosphoethanolamine,lysophosphatidylcholine,and carnitine.Additionally,PCP reversed the expression of key genes involved in fatty acid uptake,intracellular lipid transport,and fatty acid synthesis,such as fatty acid binding protein 2,fatty acid transport protein 4,apolipoprotein B,apolipoprotein C3,fatty acid synthase,long-chain fatty acyl CoA synthetase 3,lipoprotein lipase and acyl-CoA thioesterases 12(P<0.05).Conclusions These findings demonstrate that PCP mitigates PEDV-induced intestinal injury by modulating lipid metabolism and highlight its potential as a dietary supplement for enhancing anti-PEDV defenses and promoting intestinal health in piglets.
基金Supported by Natural Science Foundation of Hubei Province(2013CFB472)~~
文摘This study was conducted to extract pachyman to the greatest degree with ultrasonic technology, and to determine the content of pachyman by the phe-nol-sulfuric acid method, so as to choose the optimal technique of ultrasonic extrac-tion of pachyman. Firstly, the ultrasonic power, extraction time and solid-to-liquid ra-tio were investigated by ultrasonic extraction method. Then, on this basis, a respon-sible surface experiment was designed, to select the optimal process conditions ac-cording to the index of pachyman yield. The optimized ultrasonic water extraction conditions were as fol ow： ultrasonic power of 100 W, extraction time of 30 min, and solid-to-liquid ratio at 1：40. The Oxford cup method was used to study the an-timicrobial activity of pachyman. lt was found that pachyman could significantly in-hibit Bacilus subtilis_and Staphylococcus aureus.
基金the National Natural Science Foundation of China (30560092)
文摘To investigate the gene expression profile of endosperm development, a cDNA library was constructed and characterized from the pulp of coconut at different developmental stages. The constructed cDNA library incorporated approximately 1 × 10^7 clones in total, and the size of the insertion fragments ranged from 800 to 2 000 bp. Sequencing results of 100 randomly picked clones showed that the recombination rate was 96%. In subsequent sequence analysis, 41 clones （41%） were homologous to known function proteins, and 23 clones showed high amino acid identity （more than 80%） with the corresponding genes of different plants. Semi-quantitative RT-PCR indicated that oleosin and globulin genes are pulpspecific expression, and have differential expression level in different developmental stage. Clone 29, recognized as homologous to KIAA1239 protein （Homo sapiens）, was observed to occur nine times, indicating that this gene may be over-expressed during the endosperm development stage. However, the homologous protein was found only in mammals, and the detailed function is still unknown. Elucidation of the functional characterization of these genes will be carried out immediately.
