There is increasing evidence shows that either electrical stimulation(ES)or metal ion is an effective way to accelerate ulcerative wound healing.However,less attention is paid to investigating the synergistic effect b...There is increasing evidence shows that either electrical stimulation(ES)or metal ion is an effective way to accelerate ulcerative wound healing.However,less attention is paid to investigating the synergistic effect between them.Herein,we explore the combined effects of ES and multiple metal ions on dia-betic wound healing assisted by a triboelectric nanogenerator(TENG).Firstly,the novel Eggshell@CuFe_(2)O_(4)nanocomposites(NCs)are prepared,which show unique structure and intrinsic antimicrobial properties.Subsequently,the as-prepared nanocomposites are embedded in oxidized starch hydrogel to form a mul-tifunctional composite gel,which is further assembled into a wearable ionic triboelectric nanogenerator(iTENG)patch with polydimethylsiloxane(PDMS).It can convert the mechanical energy produced by a human body motion to electric energy and mediate the sequential release of metal ions(Fe^(2+)/Ca^(2+)/Cu^(2+)),thereby resulting in the“cocktail effect”on impaired tissue.Under their effects,a satisfying healing re-sult in diabetic mouse is identified,which can effectively accelerate wound healing process by relieving inflammation,promoting angiogenesis and collagen deposition.The work puts forward the cocktail effect of electric simulation coupled with the multiple metal ions,and opens up a new perspective in designing iTENG patch towards repair of hard-to-heal wounds.展开更多
High entropy engineering has been widely used to optimize properties of various materials,and we improve comprehensive performance of rare-earth tantalates RETaO_(4)(RE is rare earth)by changing configurational entrop...High entropy engineering has been widely used to optimize properties of various materials,and we improve comprehensive performance of rare-earth tantalates RETaO_(4)(RE is rare earth)by changing configurational entropy in this work.Four medium/high entropy RETaO_(4)(M/HERT)are successfully prepared,and the variations of disorders and distortion degree of lattices with the increasing configurational entropy are described in detail.It is revealed that M/HERT with the highest configurational entropy does not correspond to the best comprehensive properties.Unexpected variations in properties of M/HERT compared to RETaO_(4)are observed.By comparing with values obtained from rule of mixture(ROM),it is believed that the cocktail effect exists in M/HERT.The synergistic optimizations of thermomechanical properties are realized,including reducing thermal conductivity,increasing thermal expansion coefficients(TECs),and enhancing mechanical properties.M/HERT exhibit excellent high temperature stability and provide a good thermal insulation gradient,which is significant for hightemperature applications of RETaO_(4).This work serves as an important part for thermal barrier coatings materials with high working temperatures and low thermal conductivity.展开更多
High entropy alloys(HEAs)have been the star materials in electrocatalysis research in recent years.One of their key features is the greatly increased multiplicity of active sites compared to conventional catalytic mat...High entropy alloys(HEAs)have been the star materials in electrocatalysis research in recent years.One of their key features is the greatly increased multiplicity of active sites compared to conventional catalytic materials.This increased multiplicity stimulates a cocktail effect and a scaling-relation breaking effect,and results in improved activity.However,the multiplicity of active sites in HEAs also poses new problems for mechanistic studies.One apparent problem is the inapplicability to HEA catalysts of the currently most popular mechanistic study method,which uses the electrocatalytic theoretical framework(ETF)based on the computational hydrogen electrode(CHE).The ETF uses a single adsorption energy to represent the catalyst,i.e.,a catalyst is represented by a'point'in the volcanic relationship.It naturally does not involve the multiplicity of active sites of a catalyst,and hence loses brevity in expressing the cocktail effect and scaling-relation breaking effect in HEA catalysis.This paper attempts to solve this inapplicability.Based on the fact that the adsorption energy distribution of HEAs is close to a normal distribution,the mean and variance of the adsorption energy distribution are introduced as descriptors of the ETF,replacing the original single adsorption energy.A quantitative relationship between the variance and the cocktail and scaling-relation braking effects is established.We believe the method described in this work will make the ETF more effective in mechanistic studies of HEA electrocatalysis.展开更多
As promising anodes for sodium-ion batteries,metal sulfides ubiquitously suffer from low-rate and high-plateau issues,greatly hindering their application in full-cells.Herein,exemplifying carbon nanotubes(CNTs)-string...As promising anodes for sodium-ion batteries,metal sulfides ubiquitously suffer from low-rate and high-plateau issues,greatly hindering their application in full-cells.Herein,exemplifying carbon nanotubes(CNTs)-stringed metal sulfides superstructure(CSC)assembled by nano-dispersed SnS_(2) and CoS_(2) phases,cocktail mediation effect similar to that of high-entropy materials is initially studied in ether-based electrolyte to solve the challenges.The high nano-dispersity of metal sulfides in CSC anode underlies the cocktail-like mediation effect,enabling the circumvention of intrinsic drawbacks of different metal sulfides.By utilizing ether-based electrolyte,the reversibility of metal sulfides is greatly improved,sustaining a long-life effectivity of cocktail-like mediation.As such,CSC effectively overcomes low-rate flaw of SnS_(2) and highplateau demerit of CoS_(2),simultaneously realizes a high rate and a low plateau.In half-cells,CSC delivers an ultrahigh-rate capability of 327.6 mAh g^(−1) anode at 20 A g^(−1),far outperforming those of monometallic sulfides(SnS_(2),CoS_(2))and their mixtures.Compared with CoS_(2) phase and SnS_(2)/CoS_(2) mixture,CSC shows remarkably lowered average charge voltage up to ca.0.62 V.As-assembled CSC//Na1.5VPO4.8F0.7 full-cell shows a good rate capability(0.05~1.0 A g^(−1),120.3 mAh g^(−1) electrode at 0.05 A g^(−1))and a high average discharge voltage up to 2.57 V,comparable to full-cells with alloy-type anodes.Kinetics analysis verifies that the cocktail-like mediation effect largely boosts the charge transfer and ionic diffusion in CSC,compared with single phase and mixed phases.Further mechanism study reveals that alternative and complementary electrochemical processes between nano-dispersed SnS_(2) and CoS_(2) phases are responsible for the lowered charge voltage of CSC.This electrolyte/structure-dependent cocktail-like mediation effect effectively enhances the practicability of metal sulfide anodes,which will boost the development of high-rate/-voltage sodium-ion full batteries.展开更多
目的:全膝关节置换术后疼痛严重,须采用围手术期多模式镇痛,关节周围局部浸润(local infiltration analgesia,LIA)是镇痛方法之一,但作用时间较短。本研究通过线性混合效应模型比较全膝关节置换患者使用传统“鸡尾酒”疗法与加入硫酸镁...目的:全膝关节置换术后疼痛严重,须采用围手术期多模式镇痛,关节周围局部浸润(local infiltration analgesia,LIA)是镇痛方法之一,但作用时间较短。本研究通过线性混合效应模型比较全膝关节置换患者使用传统“鸡尾酒”疗法与加入硫酸镁和碳酸氢钠的改良“鸡尾酒”疗法的LIA早期镇痛疗效。方法:回顾性收集武威市人民医院骨二科2024年1月至2025年2月就诊的193例全膝关节置换术患者,以使用含有硫酸镁注射液和碳酸氢钠注射液的改良“鸡尾酒”为暴露因素,将改良“鸡尾酒”治疗患者作为实验组(n=87),传统“鸡尾酒”治疗患者作为对照组(n=106)。收集患者的一般资料,包括性别、年龄、身高、体重、体重指数(body mass index,BMI)、关节活动度(range of motion,ROM)、疼痛视觉模拟量表(Visual Analog Scale,VAS)评分、术前美国麻醉医师协会(American Society of Anesthesiologists,ASA)分级、手术侧别和手术时长。比较2组患者首次服用盐酸羟考酮缓释片的时间,术后12 h、1 d、2 d服用盐酸羟考酮缓释片的患者占比,术后住院时间;记录2组患者术后静息时和活动时VAS评分、膝ROM等重复测量数据用于线性混合效应模型分析。结果:2组共纳入193例患者。2组性别、年龄、身高、体重、BMI、ROM、VAS评分、ASA分级、手术侧别和手术时长等一般资料比较,差异均无统计学意义(均P>0.05);术后首次服用盐酸羟考酮缓释片间隔时间实验组较对照组长,差异有统计学意义(P<0.05),实验组术后12 h和1 d服用盐酸羟考酮缓释片患者占比小于对照组,差异有统计学意义(P<0.05),2组术后2 d服用盐酸羟考酮缓释片患者占比差异无统计学意义(P>0.05);术后住院时间差异无统计学意义(P>0.05)。线性混合效应模型分析结果显示:术后12~72 h实验组静息时VAS与对照组之间存在组间效应、时间效应和交互效应(均P<0.05),术后12~72 h实验组活动时VAS与对照组之间存在组间效应、时间效应和交互效应(均P<0.05),术后24~72 h实验组膝ROM和对照组之间存在时间效应、组间效应和交互效应(均P<0.05)。2组术后并发症发生率差异无统计学意义(P>0.05)。结论:与传统“鸡尾酒”疗法相比,含有硫酸镁和碳酸氢钠的改良“鸡尾酒”疗法在全膝关节置换术患者中可进一步降低术后早期疼痛,减少术后早期盐酸羟考酮缓释片服用量和改善早期膝ROM,但对术后住院时间无明显改善。展开更多
目的探讨不同细胞因子鸡尾酒诱导的树突状细胞(DC)疫苗对裸鼠移植瘤的抗肿瘤作用。方法从38名外周血干细胞移植术供者的外周血中分离单个核细胞(PBMC),诱导成未成熟DC,将人肺腺癌细胞A549总RNA电转染入DC,分别用传统细胞因子鸡尾酒(IL-6...目的探讨不同细胞因子鸡尾酒诱导的树突状细胞(DC)疫苗对裸鼠移植瘤的抗肿瘤作用。方法从38名外周血干细胞移植术供者的外周血中分离单个核细胞(PBMC),诱导成未成熟DC,将人肺腺癌细胞A549总RNA电转染入DC,分别用传统细胞因子鸡尾酒(IL-6、IL-1β、TNF-α、PGE2)和改良细胞因子鸡尾酒(IL-1β、IL-6、TNF-α、PGE2、polyⅠ∶C、CpG ODN)刺激DC成熟。致敏DC与自体T细胞混合培养,诱导产生抗原特异性细胞毒T淋巴细胞(CTL),用流式细胞术鉴定T细胞表型,ELISA法检测IFN-γ、TNF-α、IL-4、IL-5和IL-10的分泌。建立肺腺癌A549荷瘤裸鼠模型,随机分为空白对照组、未成熟组、传统组和改良组(n=5),于肿瘤接种d15、d22、d29给予相应CTL治疗,测量肿瘤体积和重量,免疫组化法和Western blotting检测肿瘤组织COX-2、VEGF、Bcl-2和Bax蛋白表达水平。结果1)CD3+CD8+T细胞表达率(%):未成熟组、传统组和改良组分别为35.00±3.24 vs 57.10±2.69 vs 63.98±1.96(P<0.05);2)IFN-γ浓度(pg/mL):3组分别为160.12±42.01 vs 263.17±55.30 vs 1 034.30±253.07(P<0.05);TNF-α浓度(pg/mL):3组分别为72.72±9.13 vs 65.20±8.03 vs 295.89±123.80(P<0.05);IL-10浓度(pg/mL):3组分别为7.26±1.76 vs 31.06±4.19 vs 44.01±12.12(P<0.05);3组IL-4、IL-5含量均未检测出,可见改良组T细胞分泌Th1型细胞因子IFN-γ和TNF-α的能力较未成熟组和传统组明显增强;3)经CTL免疫治疗后,裸鼠肿瘤体积(mm3):3组分别为512±33 vs 345±63 vs203±52(P<0.05),且改良组抑瘤率最大,为69.62%;4)改良组DC诱导的CTL明显上调Bax的表达,下调COX-2、VEGF和Bcl-2的表达。结论改良细胞因子鸡尾酒诱导肺腺癌细胞总RNA转染的DC疫苗能诱导Th1型免疫应答及产生有效的抗原特异性CTL,对肺腺癌裸鼠移植瘤具有良好的抑制作用。展开更多
Hydrogen energy is one of the ideal energy alternatives and the upstream of the hydrogen industry chain is hydrogen production,which can be achieved via the reaction of inorganic materials with water,known as hydrolys...Hydrogen energy is one of the ideal energy alternatives and the upstream of the hydrogen industry chain is hydrogen production,which can be achieved via the reaction of inorganic materials with water,known as hydrolysis.Among inorganic materials,the high hydrogen capacity for hydrolysis of MgH_(2)(15.2 wt%)makes it a promising material for hydrogen production via hydrolysis.However,the dense Mg(OH)_(2) passivation layer will block the reaction between MgH_(2) and the solution,resulting in low hydrogen yield and sluggish hydrolysis kinetics.In this work,the hydrogenyield and hydrogen generation rate of MgH_(2) are considerably enhanced by adding Ti-Zr-Fe-Mn-Cr-V high-entropy alloys(HEAs) for the first time.In particular.the MgH_(2)-3 wt% TiZrFe_(1.5)MnCrV_(0.5)(labelled as MgH_(2)-3 wt% Fe_(1.5)) composite releases 1526.70 mL/g H_(2) within 5 min at 40℃,and the final hydrolysis conversion rate reaches 95.62% within 10 min.The mean hydrogen generation rate of the MgH_(2)-3 wt% Fe_(1.5) composite is 289.16 mL/g/min,which is 2.38 times faster than that of pure MgH_(2).Meanwhile,the activation energy of the MgH_(2)-3 wt% Fe_(1.5) composite is calculated to be 12.53 kJ/mol. The density functional theory(DFT) calculation reveals that the addition of HEAs weakens the Mg-H bonds and accelerates the electron transfer between MgH_(2) and HEAs,Combined with the cocktail effect of HEAs as well as the formation of more interfaces and micro protocells,the hydrolysis performance of MgH_(2) is considerably improved.This work provides an appealing prospect for real-time hydrogen supply and offers a new effective strategy for improving the hydrolysis performance of MgH_(2).展开更多
The superconductivity in (NbTa)<sub>1-X</sub>(HfZrTi)<sub>X</sub> high-entropy alloy is analyzed using the theory of strong-coupled superconductor. It is concluded that (NbTa)<sub>1-X<...The superconductivity in (NbTa)<sub>1-X</sub>(HfZrTi)<sub>X</sub> high-entropy alloy is analyzed using the theory of strong-coupled superconductor. It is concluded that (NbTa)<sub>1-X</sub>(HfZrTi)<sub>X </sub>is a strong coupled superconductor. The variation in the superconducting transition temperature from 7.9 K to 4.6 K as x increases from 0.2 to 0.84 arises because of the decrease in electronic band width due to localization and broadening of the band. It is suggested that the decrease in electronic band width is due to crystalline randomness which gives rise to the mobility edge.展开更多
基金supported financially by the Natural Science Foundation of Shanxi Province Grants(No.202203021211157)Shanxi-Zheda Institute of Advanced Materials and Chemical Engineering(No.2023SX-FR006)The Science and Technology Major Special Plan Project“Reveal the Tile”of Quanzhou Province(No.2022NZ1).
文摘There is increasing evidence shows that either electrical stimulation(ES)or metal ion is an effective way to accelerate ulcerative wound healing.However,less attention is paid to investigating the synergistic effect between them.Herein,we explore the combined effects of ES and multiple metal ions on dia-betic wound healing assisted by a triboelectric nanogenerator(TENG).Firstly,the novel Eggshell@CuFe_(2)O_(4)nanocomposites(NCs)are prepared,which show unique structure and intrinsic antimicrobial properties.Subsequently,the as-prepared nanocomposites are embedded in oxidized starch hydrogel to form a mul-tifunctional composite gel,which is further assembled into a wearable ionic triboelectric nanogenerator(iTENG)patch with polydimethylsiloxane(PDMS).It can convert the mechanical energy produced by a human body motion to electric energy and mediate the sequential release of metal ions(Fe^(2+)/Ca^(2+)/Cu^(2+)),thereby resulting in the“cocktail effect”on impaired tissue.Under their effects,a satisfying healing re-sult in diabetic mouse is identified,which can effectively accelerate wound healing process by relieving inflammation,promoting angiogenesis and collagen deposition.The work puts forward the cocktail effect of electric simulation coupled with the multiple metal ions,and opens up a new perspective in designing iTENG patch towards repair of hard-to-heal wounds.
基金the National Key Research and Development Program of China(No.2022YFB3708600)Yunnan Fundamental Research Projects(No.202201BE070001-008)+3 种基金General Project in Yunnan Province(No.202201AT070192,202101BE070001-011)Open Project of Yunnan Precious Metals Laboratory(No.2023050240)Project of Innovation Team in Yunnan Province(No.202305AS350018)the Academician(Expert)Workstation of Yunnan Province Program(No.202305AF150005).
文摘High entropy engineering has been widely used to optimize properties of various materials,and we improve comprehensive performance of rare-earth tantalates RETaO_(4)(RE is rare earth)by changing configurational entropy in this work.Four medium/high entropy RETaO_(4)(M/HERT)are successfully prepared,and the variations of disorders and distortion degree of lattices with the increasing configurational entropy are described in detail.It is revealed that M/HERT with the highest configurational entropy does not correspond to the best comprehensive properties.Unexpected variations in properties of M/HERT compared to RETaO_(4)are observed.By comparing with values obtained from rule of mixture(ROM),it is believed that the cocktail effect exists in M/HERT.The synergistic optimizations of thermomechanical properties are realized,including reducing thermal conductivity,increasing thermal expansion coefficients(TECs),and enhancing mechanical properties.M/HERT exhibit excellent high temperature stability and provide a good thermal insulation gradient,which is significant for hightemperature applications of RETaO_(4).This work serves as an important part for thermal barrier coatings materials with high working temperatures and low thermal conductivity.
文摘High entropy alloys(HEAs)have been the star materials in electrocatalysis research in recent years.One of their key features is the greatly increased multiplicity of active sites compared to conventional catalytic materials.This increased multiplicity stimulates a cocktail effect and a scaling-relation breaking effect,and results in improved activity.However,the multiplicity of active sites in HEAs also poses new problems for mechanistic studies.One apparent problem is the inapplicability to HEA catalysts of the currently most popular mechanistic study method,which uses the electrocatalytic theoretical framework(ETF)based on the computational hydrogen electrode(CHE).The ETF uses a single adsorption energy to represent the catalyst,i.e.,a catalyst is represented by a'point'in the volcanic relationship.It naturally does not involve the multiplicity of active sites of a catalyst,and hence loses brevity in expressing the cocktail effect and scaling-relation breaking effect in HEA catalysis.This paper attempts to solve this inapplicability.Based on the fact that the adsorption energy distribution of HEAs is close to a normal distribution,the mean and variance of the adsorption energy distribution are introduced as descriptors of the ETF,replacing the original single adsorption energy.A quantitative relationship between the variance and the cocktail and scaling-relation braking effects is established.We believe the method described in this work will make the ETF more effective in mechanistic studies of HEA electrocatalysis.
基金This work was supported by Guangdong Basic and Applied Basic Research Foundation,China(No.2019A1515110980)research project from the National Natural Science Foundation of China(No.21361162004)China Scholarship Council,and CSIRO.We acknowledge Dr Yesim Gozukara,Dr Malisja de Vries,and Dr Yunxia Yang from CSIRO(Clayton)for their help with material characterization training.
文摘As promising anodes for sodium-ion batteries,metal sulfides ubiquitously suffer from low-rate and high-plateau issues,greatly hindering their application in full-cells.Herein,exemplifying carbon nanotubes(CNTs)-stringed metal sulfides superstructure(CSC)assembled by nano-dispersed SnS_(2) and CoS_(2) phases,cocktail mediation effect similar to that of high-entropy materials is initially studied in ether-based electrolyte to solve the challenges.The high nano-dispersity of metal sulfides in CSC anode underlies the cocktail-like mediation effect,enabling the circumvention of intrinsic drawbacks of different metal sulfides.By utilizing ether-based electrolyte,the reversibility of metal sulfides is greatly improved,sustaining a long-life effectivity of cocktail-like mediation.As such,CSC effectively overcomes low-rate flaw of SnS_(2) and highplateau demerit of CoS_(2),simultaneously realizes a high rate and a low plateau.In half-cells,CSC delivers an ultrahigh-rate capability of 327.6 mAh g^(−1) anode at 20 A g^(−1),far outperforming those of monometallic sulfides(SnS_(2),CoS_(2))and their mixtures.Compared with CoS_(2) phase and SnS_(2)/CoS_(2) mixture,CSC shows remarkably lowered average charge voltage up to ca.0.62 V.As-assembled CSC//Na1.5VPO4.8F0.7 full-cell shows a good rate capability(0.05~1.0 A g^(−1),120.3 mAh g^(−1) electrode at 0.05 A g^(−1))and a high average discharge voltage up to 2.57 V,comparable to full-cells with alloy-type anodes.Kinetics analysis verifies that the cocktail-like mediation effect largely boosts the charge transfer and ionic diffusion in CSC,compared with single phase and mixed phases.Further mechanism study reveals that alternative and complementary electrochemical processes between nano-dispersed SnS_(2) and CoS_(2) phases are responsible for the lowered charge voltage of CSC.This electrolyte/structure-dependent cocktail-like mediation effect effectively enhances the practicability of metal sulfide anodes,which will boost the development of high-rate/-voltage sodium-ion full batteries.
文摘目的:全膝关节置换术后疼痛严重,须采用围手术期多模式镇痛,关节周围局部浸润(local infiltration analgesia,LIA)是镇痛方法之一,但作用时间较短。本研究通过线性混合效应模型比较全膝关节置换患者使用传统“鸡尾酒”疗法与加入硫酸镁和碳酸氢钠的改良“鸡尾酒”疗法的LIA早期镇痛疗效。方法:回顾性收集武威市人民医院骨二科2024年1月至2025年2月就诊的193例全膝关节置换术患者,以使用含有硫酸镁注射液和碳酸氢钠注射液的改良“鸡尾酒”为暴露因素,将改良“鸡尾酒”治疗患者作为实验组(n=87),传统“鸡尾酒”治疗患者作为对照组(n=106)。收集患者的一般资料,包括性别、年龄、身高、体重、体重指数(body mass index,BMI)、关节活动度(range of motion,ROM)、疼痛视觉模拟量表(Visual Analog Scale,VAS)评分、术前美国麻醉医师协会(American Society of Anesthesiologists,ASA)分级、手术侧别和手术时长。比较2组患者首次服用盐酸羟考酮缓释片的时间,术后12 h、1 d、2 d服用盐酸羟考酮缓释片的患者占比,术后住院时间;记录2组患者术后静息时和活动时VAS评分、膝ROM等重复测量数据用于线性混合效应模型分析。结果:2组共纳入193例患者。2组性别、年龄、身高、体重、BMI、ROM、VAS评分、ASA分级、手术侧别和手术时长等一般资料比较,差异均无统计学意义(均P>0.05);术后首次服用盐酸羟考酮缓释片间隔时间实验组较对照组长,差异有统计学意义(P<0.05),实验组术后12 h和1 d服用盐酸羟考酮缓释片患者占比小于对照组,差异有统计学意义(P<0.05),2组术后2 d服用盐酸羟考酮缓释片患者占比差异无统计学意义(P>0.05);术后住院时间差异无统计学意义(P>0.05)。线性混合效应模型分析结果显示:术后12~72 h实验组静息时VAS与对照组之间存在组间效应、时间效应和交互效应(均P<0.05),术后12~72 h实验组活动时VAS与对照组之间存在组间效应、时间效应和交互效应(均P<0.05),术后24~72 h实验组膝ROM和对照组之间存在时间效应、组间效应和交互效应(均P<0.05)。2组术后并发症发生率差异无统计学意义(P>0.05)。结论:与传统“鸡尾酒”疗法相比,含有硫酸镁和碳酸氢钠的改良“鸡尾酒”疗法在全膝关节置换术患者中可进一步降低术后早期疼痛,减少术后早期盐酸羟考酮缓释片服用量和改善早期膝ROM,但对术后住院时间无明显改善。
文摘目的探讨不同细胞因子鸡尾酒诱导的树突状细胞(DC)疫苗对裸鼠移植瘤的抗肿瘤作用。方法从38名外周血干细胞移植术供者的外周血中分离单个核细胞(PBMC),诱导成未成熟DC,将人肺腺癌细胞A549总RNA电转染入DC,分别用传统细胞因子鸡尾酒(IL-6、IL-1β、TNF-α、PGE2)和改良细胞因子鸡尾酒(IL-1β、IL-6、TNF-α、PGE2、polyⅠ∶C、CpG ODN)刺激DC成熟。致敏DC与自体T细胞混合培养,诱导产生抗原特异性细胞毒T淋巴细胞(CTL),用流式细胞术鉴定T细胞表型,ELISA法检测IFN-γ、TNF-α、IL-4、IL-5和IL-10的分泌。建立肺腺癌A549荷瘤裸鼠模型,随机分为空白对照组、未成熟组、传统组和改良组(n=5),于肿瘤接种d15、d22、d29给予相应CTL治疗,测量肿瘤体积和重量,免疫组化法和Western blotting检测肿瘤组织COX-2、VEGF、Bcl-2和Bax蛋白表达水平。结果1)CD3+CD8+T细胞表达率(%):未成熟组、传统组和改良组分别为35.00±3.24 vs 57.10±2.69 vs 63.98±1.96(P<0.05);2)IFN-γ浓度(pg/mL):3组分别为160.12±42.01 vs 263.17±55.30 vs 1 034.30±253.07(P<0.05);TNF-α浓度(pg/mL):3组分别为72.72±9.13 vs 65.20±8.03 vs 295.89±123.80(P<0.05);IL-10浓度(pg/mL):3组分别为7.26±1.76 vs 31.06±4.19 vs 44.01±12.12(P<0.05);3组IL-4、IL-5含量均未检测出,可见改良组T细胞分泌Th1型细胞因子IFN-γ和TNF-α的能力较未成熟组和传统组明显增强;3)经CTL免疫治疗后,裸鼠肿瘤体积(mm3):3组分别为512±33 vs 345±63 vs203±52(P<0.05),且改良组抑瘤率最大,为69.62%;4)改良组DC诱导的CTL明显上调Bax的表达,下调COX-2、VEGF和Bcl-2的表达。结论改良细胞因子鸡尾酒诱导肺腺癌细胞总RNA转染的DC疫苗能诱导Th1型免疫应答及产生有效的抗原特异性CTL,对肺腺癌裸鼠移植瘤具有良好的抑制作用。
基金financed by the National Key Research and Development Program of China [2022YFB3803703]the National Natural Science Foundation of China [52071141, 52271212, 52201250, 51771056]the Interdisciplinary Innovation Program of North China Electric Power University [XM2112355]。
文摘Hydrogen energy is one of the ideal energy alternatives and the upstream of the hydrogen industry chain is hydrogen production,which can be achieved via the reaction of inorganic materials with water,known as hydrolysis.Among inorganic materials,the high hydrogen capacity for hydrolysis of MgH_(2)(15.2 wt%)makes it a promising material for hydrogen production via hydrolysis.However,the dense Mg(OH)_(2) passivation layer will block the reaction between MgH_(2) and the solution,resulting in low hydrogen yield and sluggish hydrolysis kinetics.In this work,the hydrogenyield and hydrogen generation rate of MgH_(2) are considerably enhanced by adding Ti-Zr-Fe-Mn-Cr-V high-entropy alloys(HEAs) for the first time.In particular.the MgH_(2)-3 wt% TiZrFe_(1.5)MnCrV_(0.5)(labelled as MgH_(2)-3 wt% Fe_(1.5)) composite releases 1526.70 mL/g H_(2) within 5 min at 40℃,and the final hydrolysis conversion rate reaches 95.62% within 10 min.The mean hydrogen generation rate of the MgH_(2)-3 wt% Fe_(1.5) composite is 289.16 mL/g/min,which is 2.38 times faster than that of pure MgH_(2).Meanwhile,the activation energy of the MgH_(2)-3 wt% Fe_(1.5) composite is calculated to be 12.53 kJ/mol. The density functional theory(DFT) calculation reveals that the addition of HEAs weakens the Mg-H bonds and accelerates the electron transfer between MgH_(2) and HEAs,Combined with the cocktail effect of HEAs as well as the formation of more interfaces and micro protocells,the hydrolysis performance of MgH_(2) is considerably improved.This work provides an appealing prospect for real-time hydrogen supply and offers a new effective strategy for improving the hydrolysis performance of MgH_(2).
文摘The superconductivity in (NbTa)<sub>1-X</sub>(HfZrTi)<sub>X</sub> high-entropy alloy is analyzed using the theory of strong-coupled superconductor. It is concluded that (NbTa)<sub>1-X</sub>(HfZrTi)<sub>X </sub>is a strong coupled superconductor. The variation in the superconducting transition temperature from 7.9 K to 4.6 K as x increases from 0.2 to 0.84 arises because of the decrease in electronic band width due to localization and broadening of the band. It is suggested that the decrease in electronic band width is due to crystalline randomness which gives rise to the mobility edge.
