Cockroaches are worldwide indoor pests carrying microorganisms of medical importance. German cockroaches （Blattella germanica） were sampled in five habitats （hospital, restaurant, office home, and market） in Beiji...Cockroaches are worldwide indoor pests carrying microorganisms of medical importance. German cockroaches （Blattella germanica） were sampled in five habitats （hospital, restaurant, office home, and market） in Beijing, and the bacteria were isolated from their external surface and alimentary tract and identified using a Biolog identification system. Cockroach densities significantly differed among habitats （market 〉 home 〉 office 〉 restaurant 〉 hospital）. However, no significant differences in bacterial abundance carried by individual German cockroaches （of either sex） were found among habitats. The bacterial abundance in the gut was significantly higher than that on the surface. There were no significant differences in bacterial species richness observed among habitats, sex, carrying position or their interaction. Cluster analysis showed that cockroach densities and bacterial abundance found in the market differed significantly from the other four habitats. The bacterial diversity was not significantly reduced in sensitive facilities such as hospital and restaurant, even though pesticide and bactericide were more frequently applied there. The implications of these findings were discussed in this article.展开更多
A study was performed to estimate the prevalence of the external bacterial flora of two domestic cockroaches （Blattella germanica and Blatta orientalis） collected from households in Tebessa （northeast AIgeria）.Thr...A study was performed to estimate the prevalence of the external bacterial flora of two domestic cockroaches （Blattella germanica and Blatta orientalis） collected from households in Tebessa （northeast AIgeria）.Three major bacterial groups were cultured （total aerobic, enterobacteria, and staphylococci） from 14 specimens of cockroaches, and antibiotic susceptibility was tested for both Staphylococcus and Pseudomonas isolates. Culturing showed that the total bacterial load of cockroaches from different households were comparable （P〈0.001） and enterobacteria were the predominant colonizers of the insect surface, with a bacterial load of （2.1×10^5 CFU/insect）, whereas the staphylococci group was the minority. Twenty-eight bacterial species were isolated, and susceptibility patterns showed that most of the staphylococci isolates were highly susceptible to chloramphenicol, gentamycin, pristinamycin, ofloxacin, clindamycin, and vancomycin; however, Pseudomonas strains exhibited resistance to amoxicillin/clavulanic acid, imipenem, and the second-generation antibiotic cephalosporin cefuroxime.展开更多
Antennal sensilla are investigated in three xylophagous cockroaches： Cryptocercus meridianus, C. habaensis and C. changbaiensis, using scanning electron microscopy （SEM）. General morphology of the antenna is provid...Antennal sensilla are investigated in three xylophagous cockroaches： Cryptocercus meridianus, C. habaensis and C. changbaiensis, using scanning electron microscopy （SEM）. General morphology of the antenna is provided in detail, and no significant differences are found among the three species. Six primary types of sensilla are observed and measured： sensilla chaetica （Ch）, sensilla trichodea （Tr）, sensilla basiconica （Ba）, sensilla coeloconica （Co）, sensilla campaniformia （Ca） and sensilla capitula （Cp）. Sensilla trichodea 1 （Trl） is the most abundant sensillum in all three species. There are some statistical differences between sexes and among species. Comparing Cryptocercus with other cockroaches and termites, we briefly discuss the differences among them in terms of their habitat. According to the statistical analysis performed with the sensilla, these morphological characteristics are not enough to separate the three species and may not be suitable for a classification at species level.展开更多
This article describes the structure of the cockroach-like robot.Both kinematics and locomotion control are inspired by biological observations in cockroaches.Based on cockroach-like robot kinematics analysis,screw th...This article describes the structure of the cockroach-like robot.Both kinematics and locomotion control are inspired by biological observations in cockroaches.Based on cockroach-like robot kinematics analysis,screw theory,and the production-of-exponential (POE) formula,this paper focuses on the inverse kinematics which uses Paden-Kahan sub-problems to obtain directly the displacement of joint angles.The forward kinematics derives the relationship between joint angles according to the natural restrictions.Then,by using the POE formula,it can deduce the body pose and realize online trajectory control and planning.Through simulation and experimentation,it is proved that the straight-line walking and turning gait algorithms have static stability and the inverse kinematics analysis of cockroach-like robot is correct.展开更多
The book, Cockroaches of Southeastern China, wrote by Xianwei Liu, Weibing Zhu, Li Dai and Hanqiang Wang, was published in 2017.In this book,the cockroachs fauna of the Southern China was observed,occupied Shandong, A...The book, Cockroaches of Southeastern China, wrote by Xianwei Liu, Weibing Zhu, Li Dai and Hanqiang Wang, was published in 2017.In this book,the cockroachs fauna of the Southern China was observed,occupied Shandong, Anhui,Jiangsu, Shanghai, Zhejiang, Fujian, Jiangxi, Taiwan, Guangdong and Hainan Provinces.展开更多
Random amplified polymorphic DNA （RAPD） analysis was conducted for the differentiation of two most commonly occurring insect species Periplaneta americana and Blatella germanicana. This technique is proved to be a q...Random amplified polymorphic DNA （RAPD） analysis was conducted for the differentiation of two most commonly occurring insect species Periplaneta americana and Blatella germanicana. This technique is proved to be a quick and effective to establish genetic markers to differentiate morphologically similar populations. During the study cockroach species Periplanata americana and Blatella germanicana were considered. Ten random primers were used for polymerase chain reaction （PCR）. Many of such bands obtained, which differentiate between the two species. On the basis of interpretability, simplicity and reproducibility, six primers P1 （GATGACCGCC）, P3 （GGCACGTAAC）, P6 （GGTGCGCCTT）, P7 （GTCAGAGTCG）, P8 （GTCGCCGTCT） and PI0 （GTGCCCGATG） were considered positive for genetic differentiation and analysis. A series of bands ranging from -300 bp to -1,000 bp obtained indicates that these two species are related, however they exhibit some variations. It has also been observed that the same primers also amplified some DNA fragments of the same size in both the species, which indicates the presence of conserved regions, sharing ancestral relationship. Some of the fragments were unique in both the species which may be used for diagnostic purposes. The study concludes that the RAPD-PCR technique is useful for the study of molecular taxonomy in insects.展开更多
Cockroaches pose a significant global public health concern.However,besides the well-recognized cockroach-induced allergy,the potential impact of the cockroach microbiome on human health through various means is not y...Cockroaches pose a significant global public health concern.However,besides the well-recognized cockroach-induced allergy,the potential impact of the cockroach microbiome on human health through various means is not yet fully elucidated.This study aimed to clarify the health impacts of cockroaches by investigating the microbial interactions among cockroaches,the indoor environment,and humans.We simultaneously collected cockroach,indoor environment(indoor air and floor dust),and human(exhaled breath condensate and skin)samples from residential areas in five cities representing distinct climate zones in China.The 16S rDNA sequencing results revealed that cockroaches harbor diverse bacterial populations that vary across different cities.The prevalence of potential pathogenic bacteria(PPB)in cockroaches ranged from 1.1%to 58.9%,with dominant resistance genes conferring resistance to tetracycline,macrolide,and beta-lactam.The relationships between the cockroach microbiome and the associated environmental and human microbiomes were explored by using fast expectation-maximization microbial source tracking(FEAST).The potential contribution of cockroach bacteria to the floor dust-borne microbiome and indoor airborne microbiome was estimated to be 5.6%and 1.3%,respectively.Similarly,the potential contribution of cockroach PPB to the floor dust-borne microbiome and indoor airborne microbiome was calculated to be 4.0%and 1.2%,respectively.In residences with cockroach infestations,the contribution of other sources to the indoor environment was slightly increased.Collectively,the role of cockroaches in the transmission of microorganisms,particularly pathogenic bacteria and antibiotic resistance genes,cannot be overlooked.展开更多
Cockroaches, commonly found in urban dwellings worldwide, have long been considered vectors of various infectious diseases and cockroach allergens are one of the major etiologic risk factors for IgE-mediated allergic ...Cockroaches, commonly found in urban dwellings worldwide, have long been considered vectors of various infectious diseases and cockroach allergens are one of the major etiologic risk factors for IgE-mediated allergic respiratory illness throughout the world. A high prevalence of cockroach hypersensitivity in atopic （20-55 %） and asthmatic （49-60 %） populations has been documented. Cockroach allergens with molecular weights ranging from 6 to 120 kD have been identified by various standard immunochemical techniques. This article covers the characteristics of major cockroach allergens that have been purified, sequenced, cloned, and produced as recombinant proteins. Cellular ＆ Molecular Immunology. 2005;2（3）： 177-180.展开更多
Background Cockroaches are an important indoor allergen source causing allergic rhinitis and asthma. The aim of this study was to investigate the cockroach prevalence in mainland of China and the cross-reactivity of I...Background Cockroaches are an important indoor allergen source causing allergic rhinitis and asthma. The aim of this study was to investigate the cockroach prevalence in mainland of China and the cross-reactivity of IgE between cockroach and house dust mite allergen in Chinese patients.Methods The cockroach sensitization pattern was based on a skin prick test （SPT） obtained from a national multicenter prevalence study, in which 6304 patients from 25 allergy centers across China participated. Factors, including different regions of China, age, gender and the correlations between the American and German cockroaches and house dust mite Der p were investigated. Eighteen out of 1236 clinical sera from south China were selected to perform the cross-inhibition assay between house dust mites and cockroaches.Results Totally 25.7% of patients were SPT positive to the American cockroach （Periplaneta Americana, Per a） and 18.7% SPT positive to the German cockroach （Blattella germanica, Bla g）. The prevalence of positive cockroach SPT was higher in southern than in northern China, higher in adults than in children, and higher in males than in females.Patients had relatively low levels of cockroach SPT reactions, mainly class 1 or 2. Of the SPT positive cockroach patients,88% were also SPT positive to house dust mite （Dermatophagoides pteronyssinus, Der p）. An IgE cross-inhibition study confirmed that Der P sensitization could cause false positive SPT reactions against cockroach.Conclusions A relatively high prevalence of cockroach sensitivity was found in mainland of China. However, a cross-inhibition study showed that only a small number of patients appear to have Bla g and/or Per a as primary sensitizing source. The importance of cockroaches as a risk factor for sensitization and triggers of allergic symptoms in mainland of China needs to be further investigated.展开更多
Volatile sex pheromones are vital for sexual communication between males and females.Females of the American cockroach,Periplaneta americana,produce and emit two sex pheromone components,periplanone-A(PA)and periplano...Volatile sex pheromones are vital for sexual communication between males and females.Females of the American cockroach,Periplaneta americana,produce and emit two sex pheromone components,periplanone-A(PA)and periplanone-B(PB).Although PB is the major sex attractant and can attract males,how it interacts with PA in regulating sexual behaviors is still unknown.In this study,we found that in male cockroaches,PA counteracted PB attraction.We identified two odorant receptors(ORs),OR53 and OR100,as PB/PA and PA receptors,respectively.OR53 and OR100 were predominantly expressed in the antennae of sexually mature males,and their expression levels were regulated by the sex differentiation pathway and nutrition-responsive signals.Cellular localization of OR53 and OR100 in male antennae further revealed that two types of sensilla coordinate a complex two-pheromone-two-receptor pathway in regulating cockroach sexual behaviors.These findings indicate distinct functions of the two sex pheromone components,identify their receptors and possible regulatory mechanisms underlying the male-specific and age-dependent sexual behaviors,and can guide novel strategies for pest management.展开更多
Traditional food packaging films made of plastic have caused serious damage to the environment.Chitosan film is a potential substitute but it is weak in antioxidant activity.In this study,the extract and chitosan from...Traditional food packaging films made of plastic have caused serious damage to the environment.Chitosan film is a potential substitute but it is weak in antioxidant activity.In this study,the extract and chitosan from American cockroach were combined to produce a new environment-friendly chitosan film for food packaging.The chitosan film was easily degraded by water,avoiding accumulation in the environment.The addition of American cockroach extract improved the antioxidant activity of the chitosan film(up to 50 times higher than that of pure chitosan film when considering reducing capacity)and did not weaken its efficient antibacterial activity.In practical application,the packaged food was protected by the chitosan film from fast decay and oxidation for 15 days and 48 h,respectively.These results suggested that the new chitosan film possessed the potential for food packaging and improved the value of American cockroach.展开更多
Vrsansky, （2000） considered there is decreasing variability in the forewings of cockroaches through time, based on a study of fossil and Recent species. This study tests his theory, based on a study of four Recent s...Vrsansky, （2000） considered there is decreasing variability in the forewings of cockroaches through time, based on a study of fossil and Recent species. This study tests his theory, based on a study of four Recent species of cockroaches： Blattella germanica （Blattellidae）, Polyphaga aegyptiaca （Polyphagidae）, Shelfordella lateralis （Blattidae） and Blaberus craniifer （Blaberidae）. The new results, based on the coefficient of variation （CV） of the number of veins, indicate that Recent species can be more variable than fossil species and therefore do not support the theory. The results also show that at least 30 complete wings of the same species are required for a reliable CV value that is compa- rable between species.展开更多
Cariblattoides labandeirai sp.n. from the Eocene sediments of Green River in Colorado, USA bear only two plesiomorphies, but also several significant autapomorphies within the advanced and highly derived living cockro...Cariblattoides labandeirai sp.n. from the Eocene sediments of Green River in Colorado, USA bear only two plesiomorphies, but also several significant autapomorphies within the advanced and highly derived living cockroach genus. Thus, Cariblattoides with extant occurrence in the Caribbean and South America was historically common in the Nearctic, and represents important evidence for the occurrence of derived living genera of cockroaches -50 Ma ago. Generally, the vast majority of living genera were absent during the Palaeocene, thus the diversification of most living cockroach lineages near the Palaeocene/Eocene boundary must have been extremely rapid. Females of living C. suave, the type species, have identical （sophisticated） coloration ofpronotum, but the most related living taxa are C. piraiensis and C.fontesi from Brazil （supported by phylogenetical analysis）.展开更多
Parthenogenesis is an asexual mode of reproduction that plays an important role in the evolution of sex, sociality, and reproduction strategies in insects. Some species of cockroach exhibit thelytoky, a type of parthe...Parthenogenesis is an asexual mode of reproduction that plays an important role in the evolution of sex, sociality, and reproduction strategies in insects. Some species of cockroach exhibit thelytoky, a type of parthenogenesis in which female offspring are produced without fertilization. However, the cytological and genetic mecha? nisms of parthenogenesis in cockroaches are not well understood. Here we provide the first molecular genetic evidence that cockroaches can reproduce through automixis. Using the American cockroach Periplaneta aniericana, we performed microsatellite analysis to investigate the genetic relationship between parthenogenetically produced nymphs and the parent virgin females, and found that all parthenogenetic offspring were homozygous for autosomal microsatellite markers, whereas the female parents were heterozygous. In addition, flow cytometry analysis revealed that the parthenogenetic offspring were diploid. Taken together, our results demonstrate that P. americana exhibits automixis-type thelytoky, in which diploidy is restored by gamete duplication or terminal fusion. These findings highlight the unique reproduction strategies of cockroaches, which are more varied than was previously recognized.展开更多
基金supported by the Knowledge Innovation Program of CAS (No. KZCX2-YW-422)the Open Fund of State Key Laboratory of Urban and Regional Ecology.
文摘Cockroaches are worldwide indoor pests carrying microorganisms of medical importance. German cockroaches （Blattella germanica） were sampled in five habitats （hospital, restaurant, office home, and market） in Beijing, and the bacteria were isolated from their external surface and alimentary tract and identified using a Biolog identification system. Cockroach densities significantly differed among habitats （market 〉 home 〉 office 〉 restaurant 〉 hospital）. However, no significant differences in bacterial abundance carried by individual German cockroaches （of either sex） were found among habitats. The bacterial abundance in the gut was significantly higher than that on the surface. There were no significant differences in bacterial species richness observed among habitats, sex, carrying position or their interaction. Cluster analysis showed that cockroach densities and bacterial abundance found in the market differed significantly from the other four habitats. The bacterial diversity was not significantly reduced in sensitive facilities such as hospital and restaurant, even though pesticide and bactericide were more frequently applied there. The implications of these findings were discussed in this article.
文摘A study was performed to estimate the prevalence of the external bacterial flora of two domestic cockroaches （Blattella germanica and Blatta orientalis） collected from households in Tebessa （northeast AIgeria）.Three major bacterial groups were cultured （total aerobic, enterobacteria, and staphylococci） from 14 specimens of cockroaches, and antibiotic susceptibility was tested for both Staphylococcus and Pseudomonas isolates. Culturing showed that the total bacterial load of cockroaches from different households were comparable （P〈0.001） and enterobacteria were the predominant colonizers of the insect surface, with a bacterial load of （2.1×10^5 CFU/insect）, whereas the staphylococci group was the minority. Twenty-eight bacterial species were isolated, and susceptibility patterns showed that most of the staphylococci isolates were highly susceptible to chloramphenicol, gentamycin, pristinamycin, ofloxacin, clindamycin, and vancomycin; however, Pseudomonas strains exhibited resistance to amoxicillin/clavulanic acid, imipenem, and the second-generation antibiotic cephalosporin cefuroxime.
基金supported by the Natural Science Foundation Project of Chongqing (cstc2014jcyj80018)
文摘Antennal sensilla are investigated in three xylophagous cockroaches： Cryptocercus meridianus, C. habaensis and C. changbaiensis, using scanning electron microscopy （SEM）. General morphology of the antenna is provided in detail, and no significant differences are found among the three species. Six primary types of sensilla are observed and measured： sensilla chaetica （Ch）, sensilla trichodea （Tr）, sensilla basiconica （Ba）, sensilla coeloconica （Co）, sensilla campaniformia （Ca） and sensilla capitula （Cp）. Sensilla trichodea 1 （Trl） is the most abundant sensillum in all three species. There are some statistical differences between sexes and among species. Comparing Cryptocercus with other cockroaches and termites, we briefly discuss the differences among them in terms of their habitat. According to the statistical analysis performed with the sensilla, these morphological characteristics are not enough to separate the three species and may not be suitable for a classification at species level.
基金the National High Technology Research and Development Program (863) of China(No. 2008AA04Z210)the National Natural Science Foundation of China(No. 60775059)the Natural Science Foundation of Beijing(No. 3093021)
文摘This article describes the structure of the cockroach-like robot.Both kinematics and locomotion control are inspired by biological observations in cockroaches.Based on cockroach-like robot kinematics analysis,screw theory,and the production-of-exponential (POE) formula,this paper focuses on the inverse kinematics which uses Paden-Kahan sub-problems to obtain directly the displacement of joint angles.The forward kinematics derives the relationship between joint angles according to the natural restrictions.Then,by using the POE formula,it can deduce the body pose and realize online trajectory control and planning.Through simulation and experimentation,it is proved that the straight-line walking and turning gait algorithms have static stability and the inverse kinematics analysis of cockroach-like robot is correct.
文摘The book, Cockroaches of Southeastern China, wrote by Xianwei Liu, Weibing Zhu, Li Dai and Hanqiang Wang, was published in 2017.In this book,the cockroachs fauna of the Southern China was observed,occupied Shandong, Anhui,Jiangsu, Shanghai, Zhejiang, Fujian, Jiangxi, Taiwan, Guangdong and Hainan Provinces.
文摘Random amplified polymorphic DNA （RAPD） analysis was conducted for the differentiation of two most commonly occurring insect species Periplaneta americana and Blatella germanicana. This technique is proved to be a quick and effective to establish genetic markers to differentiate morphologically similar populations. During the study cockroach species Periplanata americana and Blatella germanicana were considered. Ten random primers were used for polymerase chain reaction （PCR）. Many of such bands obtained, which differentiate between the two species. On the basis of interpretability, simplicity and reproducibility, six primers P1 （GATGACCGCC）, P3 （GGCACGTAAC）, P6 （GGTGCGCCTT）, P7 （GTCAGAGTCG）, P8 （GTCGCCGTCT） and PI0 （GTGCCCGATG） were considered positive for genetic differentiation and analysis. A series of bands ranging from -300 bp to -1,000 bp obtained indicates that these two species are related, however they exhibit some variations. It has also been observed that the same primers also amplified some DNA fragments of the same size in both the species, which indicates the presence of conserved regions, sharing ancestral relationship. Some of the fragments were unique in both the species which may be used for diagnostic purposes. The study concludes that the RAPD-PCR technique is useful for the study of molecular taxonomy in insects.
基金supported by the National Key Research and Development Program of China(2023YFC3708201)Beijing Natural Science Foundation-Xiaomi Innovation Joint Fund(Frontier Project L233014)National Natural Science Foundation of China(42075117&42375113).
文摘Cockroaches pose a significant global public health concern.However,besides the well-recognized cockroach-induced allergy,the potential impact of the cockroach microbiome on human health through various means is not yet fully elucidated.This study aimed to clarify the health impacts of cockroaches by investigating the microbial interactions among cockroaches,the indoor environment,and humans.We simultaneously collected cockroach,indoor environment(indoor air and floor dust),and human(exhaled breath condensate and skin)samples from residential areas in five cities representing distinct climate zones in China.The 16S rDNA sequencing results revealed that cockroaches harbor diverse bacterial populations that vary across different cities.The prevalence of potential pathogenic bacteria(PPB)in cockroaches ranged from 1.1%to 58.9%,with dominant resistance genes conferring resistance to tetracycline,macrolide,and beta-lactam.The relationships between the cockroach microbiome and the associated environmental and human microbiomes were explored by using fast expectation-maximization microbial source tracking(FEAST).The potential contribution of cockroach bacteria to the floor dust-borne microbiome and indoor airborne microbiome was estimated to be 5.6%and 1.3%,respectively.Similarly,the potential contribution of cockroach PPB to the floor dust-borne microbiome and indoor airborne microbiome was calculated to be 4.0%and 1.2%,respectively.In residences with cockroach infestations,the contribution of other sources to the indoor environment was slightly increased.Collectively,the role of cockroaches in the transmission of microorganisms,particularly pathogenic bacteria and antibiotic resistance genes,cannot be overlooked.
文摘Cockroaches, commonly found in urban dwellings worldwide, have long been considered vectors of various infectious diseases and cockroach allergens are one of the major etiologic risk factors for IgE-mediated allergic respiratory illness throughout the world. A high prevalence of cockroach hypersensitivity in atopic （20-55 %） and asthmatic （49-60 %） populations has been documented. Cockroach allergens with molecular weights ranging from 6 to 120 kD have been identified by various standard immunochemical techniques. This article covers the characteristics of major cockroach allergens that have been purified, sequenced, cloned, and produced as recombinant proteins. Cellular ＆ Molecular Immunology. 2005;2（3）： 177-180.
文摘Background Cockroaches are an important indoor allergen source causing allergic rhinitis and asthma. The aim of this study was to investigate the cockroach prevalence in mainland of China and the cross-reactivity of IgE between cockroach and house dust mite allergen in Chinese patients.Methods The cockroach sensitization pattern was based on a skin prick test （SPT） obtained from a national multicenter prevalence study, in which 6304 patients from 25 allergy centers across China participated. Factors, including different regions of China, age, gender and the correlations between the American and German cockroaches and house dust mite Der p were investigated. Eighteen out of 1236 clinical sera from south China were selected to perform the cross-inhibition assay between house dust mites and cockroaches.Results Totally 25.7% of patients were SPT positive to the American cockroach （Periplaneta Americana, Per a） and 18.7% SPT positive to the German cockroach （Blattella germanica, Bla g）. The prevalence of positive cockroach SPT was higher in southern than in northern China, higher in adults than in children, and higher in males than in females.Patients had relatively low levels of cockroach SPT reactions, mainly class 1 or 2. Of the SPT positive cockroach patients,88% were also SPT positive to house dust mite （Dermatophagoides pteronyssinus, Der p）. An IgE cross-inhibition study confirmed that Der P sensitization could cause false positive SPT reactions against cockroach.Conclusions A relatively high prevalence of cockroach sensitivity was found in mainland of China. However, a cross-inhibition study showed that only a small number of patients appear to have Bla g and/or Per a as primary sensitizing source. The importance of cockroaches as a risk factor for sensitization and triggers of allergic symptoms in mainland of China needs to be further investigated.
基金supported by the National Natural Science Foundation of China(32220103003,31930014,31900355,31970943 and 32000334)the Department of Science and Technology in Guangdong Province(2022A1515011759)+2 种基金the Laboratory of Lingnan Modern Agriculture Project(NT2021003)by the Department of Science and Technology in Guangdong Province(2019B090905003)by the Shenzhen Science and Technology Program(KQTD20180411143628272)。
文摘Volatile sex pheromones are vital for sexual communication between males and females.Females of the American cockroach,Periplaneta americana,produce and emit two sex pheromone components,periplanone-A(PA)and periplanone-B(PB).Although PB is the major sex attractant and can attract males,how it interacts with PA in regulating sexual behaviors is still unknown.In this study,we found that in male cockroaches,PA counteracted PB attraction.We identified two odorant receptors(ORs),OR53 and OR100,as PB/PA and PA receptors,respectively.OR53 and OR100 were predominantly expressed in the antennae of sexually mature males,and their expression levels were regulated by the sex differentiation pathway and nutrition-responsive signals.Cellular localization of OR53 and OR100 in male antennae further revealed that two types of sensilla coordinate a complex two-pheromone-two-receptor pathway in regulating cockroach sexual behaviors.These findings indicate distinct functions of the two sex pheromone components,identify their receptors and possible regulatory mechanisms underlying the male-specific and age-dependent sexual behaviors,and can guide novel strategies for pest management.
基金supported by the National Natural Science Foundation of China(Nos.31830084,32070466&31970440)“the Fundamental Research Funds for the Central Universities”,Nankai University(Nos.96172158,96173250&91822294).
文摘Traditional food packaging films made of plastic have caused serious damage to the environment.Chitosan film is a potential substitute but it is weak in antioxidant activity.In this study,the extract and chitosan from American cockroach were combined to produce a new environment-friendly chitosan film for food packaging.The chitosan film was easily degraded by water,avoiding accumulation in the environment.The addition of American cockroach extract improved the antioxidant activity of the chitosan film(up to 50 times higher than that of pure chitosan film when considering reducing capacity)and did not weaken its efficient antibacterial activity.In practical application,the packaged food was protected by the chitosan film from fast decay and oxidation for 15 days and 48 h,respectively.These results suggested that the new chitosan film possessed the potential for food packaging and improved the value of American cockroach.
文摘Vrsansky, （2000） considered there is decreasing variability in the forewings of cockroaches through time, based on a study of fossil and Recent species. This study tests his theory, based on a study of four Recent species of cockroaches： Blattella germanica （Blattellidae）, Polyphaga aegyptiaca （Polyphagidae）, Shelfordella lateralis （Blattidae） and Blaberus craniifer （Blaberidae）. The new results, based on the coefficient of variation （CV） of the number of veins, indicate that Recent species can be more variable than fossil species and therefore do not support the theory. The results also show that at least 30 complete wings of the same species are required for a reliable CV value that is compa- rable between species.
文摘Cariblattoides labandeirai sp.n. from the Eocene sediments of Green River in Colorado, USA bear only two plesiomorphies, but also several significant autapomorphies within the advanced and highly derived living cockroach genus. Thus, Cariblattoides with extant occurrence in the Caribbean and South America was historically common in the Nearctic, and represents important evidence for the occurrence of derived living genera of cockroaches -50 Ma ago. Generally, the vast majority of living genera were absent during the Palaeocene, thus the diversification of most living cockroach lineages near the Palaeocene/Eocene boundary must have been extremely rapid. Females of living C. suave, the type species, have identical （sophisticated） coloration ofpronotum, but the most related living taxa are C. piraiensis and C.fontesi from Brazil （supported by phylogenetical analysis）.
文摘Parthenogenesis is an asexual mode of reproduction that plays an important role in the evolution of sex, sociality, and reproduction strategies in insects. Some species of cockroach exhibit thelytoky, a type of parthenogenesis in which female offspring are produced without fertilization. However, the cytological and genetic mecha? nisms of parthenogenesis in cockroaches are not well understood. Here we provide the first molecular genetic evidence that cockroaches can reproduce through automixis. Using the American cockroach Periplaneta aniericana, we performed microsatellite analysis to investigate the genetic relationship between parthenogenetically produced nymphs and the parent virgin females, and found that all parthenogenetic offspring were homozygous for autosomal microsatellite markers, whereas the female parents were heterozygous. In addition, flow cytometry analysis revealed that the parthenogenetic offspring were diploid. Taken together, our results demonstrate that P. americana exhibits automixis-type thelytoky, in which diploidy is restored by gamete duplication or terminal fusion. These findings highlight the unique reproduction strategies of cockroaches, which are more varied than was previously recognized.
