针对巴西龟(Trachemys scripta elegans)在广东养殖过程中发生慢性死亡的现象,对病龟肠道病理组织学和病原学进行了研究,并对病龟开展了防治实验。结果表明:病龟肠道前中段上皮细胞有虫体寄生,分离到圆形至椭圆形的卵囊,平均大小为11.7...针对巴西龟(Trachemys scripta elegans)在广东养殖过程中发生慢性死亡的现象,对病龟肠道病理组织学和病原学进行了研究,并对病龟开展了防治实验。结果表明:病龟肠道前中段上皮细胞有虫体寄生,分离到圆形至椭圆形的卵囊,平均大小为11.7μm×13.8μm。每个孢子化卵囊含4个孢子囊,每个孢子囊内有2个子孢子;经口人工感染的幼龟肠道中段粘膜层有病原侵入,并出现纤维素性炎症。通过分析确认此病症为艾美耳球虫(Eimeria coccidium)感染所致。对病龟采用抗球虫药磺胺氯吡嗪进行治疗,有一定的治疗效果。展开更多
The experiments were carried out to investigate the optimum immune age and immune dosage of GC02 coccidian vaccine.Ninety healthy cross broilers were chosen and randomly divided intoⅠ,Ⅱ,Ⅲ,Ⅳ,Ⅴ,Ⅵ groups（n=15） to...The experiments were carried out to investigate the optimum immune age and immune dosage of GC02 coccidian vaccine.Ninety healthy cross broilers were chosen and randomly divided intoⅠ,Ⅱ,Ⅲ,Ⅳ,Ⅴ,Ⅵ groups（n=15） to study the immune effect of different immune dosage at the same immune times.Each chicken in Ⅰ-Ⅳ groups were orally inoculated with 4 000,8 000,16 000,20 000 sporulated oocysts of GC02 coccidian vaccine,respectively.Chickens in Ⅴ group were not infected with sporulated oocysts and chickens in Ⅵ group were not inoculated.On the 7th day after the second immunity,Ⅰ-Ⅳgroups were（orally） challenged with 1.0×105 sporulated oocysts for each chicken.On the 8th day after challenging with the sporulated oocysts,all chicken were slaughtered.According to lesion scoring and faces scoring,OPG,ACI,relative growth rate,feed conversion,etc.,the effects of different immune dosage of GC02 coccidian vaccine were evaluated.The results suggested that optimum immunization method was inoculating 8 000 sporulated oocysts per bird two times.One-hundred and eighty healthy chickens were randomly divided into A,B,C,D,E,F,G1,G2,G3 groups（n=20） to study the immune effects of different immune age with the same immune dosage.Chickens in A,B,C groups were immunized with GC02 coccidian vaccine at the different immune age;D,E,F and G1,G2,G3 were positive and negative control groups respectively,the immune and challenge is the same as experiment one.The results concluded that GC02 coccidian vaccine is safe and effectual in preventing coccidiosis,immunize with 8 000 sporalated oocysts per bird at the age of 7 days and 14 days can obtain the ideal effects.展开更多
文摘针对巴西龟(Trachemys scripta elegans)在广东养殖过程中发生慢性死亡的现象,对病龟肠道病理组织学和病原学进行了研究,并对病龟开展了防治实验。结果表明:病龟肠道前中段上皮细胞有虫体寄生,分离到圆形至椭圆形的卵囊,平均大小为11.7μm×13.8μm。每个孢子化卵囊含4个孢子囊,每个孢子囊内有2个子孢子;经口人工感染的幼龟肠道中段粘膜层有病原侵入,并出现纤维素性炎症。通过分析确认此病症为艾美耳球虫(Eimeria coccidium)感染所致。对病龟采用抗球虫药磺胺氯吡嗪进行治疗,有一定的治疗效果。
文摘The experiments were carried out to investigate the optimum immune age and immune dosage of GC02 coccidian vaccine.Ninety healthy cross broilers were chosen and randomly divided intoⅠ,Ⅱ,Ⅲ,Ⅳ,Ⅴ,Ⅵ groups（n=15） to study the immune effect of different immune dosage at the same immune times.Each chicken in Ⅰ-Ⅳ groups were orally inoculated with 4 000,8 000,16 000,20 000 sporulated oocysts of GC02 coccidian vaccine,respectively.Chickens in Ⅴ group were not infected with sporulated oocysts and chickens in Ⅵ group were not inoculated.On the 7th day after the second immunity,Ⅰ-Ⅳgroups were（orally） challenged with 1.0×105 sporulated oocysts for each chicken.On the 8th day after challenging with the sporulated oocysts,all chicken were slaughtered.According to lesion scoring and faces scoring,OPG,ACI,relative growth rate,feed conversion,etc.,the effects of different immune dosage of GC02 coccidian vaccine were evaluated.The results suggested that optimum immunization method was inoculating 8 000 sporulated oocysts per bird two times.One-hundred and eighty healthy chickens were randomly divided into A,B,C,D,E,F,G1,G2,G3 groups（n=20） to study the immune effects of different immune age with the same immune dosage.Chickens in A,B,C groups were immunized with GC02 coccidian vaccine at the different immune age;D,E,F and G1,G2,G3 were positive and negative control groups respectively,the immune and challenge is the same as experiment one.The results concluded that GC02 coccidian vaccine is safe and effectual in preventing coccidiosis,immunize with 8 000 sporalated oocysts per bird at the age of 7 days and 14 days can obtain the ideal effects.
