Peter Coad是我喜欢的一位面向对象专家和软件创业者。他在上世纪九十年代与人合著了6本关于面向对象软件的分析、设计和编程的书籍，以《面向对象分析》一书中和Yourdon共创的Coad／Yourdon方法而成名。1999年，他创建了TogetherSoft...Peter Coad是我喜欢的一位面向对象专家和软件创业者。他在上世纪九十年代与人合著了6本关于面向对象软件的分析、设计和编程的书籍，以《面向对象分析》一书中和Yourdon共创的Coad／Yourdon方法而成名。1999年，他创建了TogetherSoft公司。2003年，TogetherSoft卖给了Borland公司，他成为了Borland公司的副总裁。展开更多
Background:Colorectal adenocarcinoma(COAD)is one of the most common gastrointestinal malignancies.There is a pressing need to recognize reliable biomarkers that can improve diagnostic accuracy,predict prognosis,and se...Background:Colorectal adenocarcinoma(COAD)is one of the most common gastrointestinal malignancies.There is a pressing need to recognize reliable biomarkers that can improve diagnostic accuracy,predict prognosis,and serve as effective molecular targets.Glutathione peroxidase 4(GPX4)is an important antioxidant protein.Evidence demonstrates that abnormal expression of GPX4 is related to cancer initiation and progression.However,the role of GPX4 in COAD remains unclear.Methods:We employed bioinformatics analysis and conducted subsequent validation of biological processes,including cell counting kit-8 assay(CCK-8),colony formation assay,reverse transcription-quantitative polymerase chain reaction(RT-qPCR),5-ethynyl-2′-deoxyuridine assay(EdU),western blot,immunohistochemistry,senescence associatedβ-galactosidase(SA-β-gal)staining and immunofluorescence to explore the expression status,prognostic value and biological function of GPX4 in COAD.Results:Our data revealed that GPX4 mRNA expression was upregulated in COAD tissues and could predict the prognosis in patients with COAD.High GPX4 expression was associated with increased infiltration of malignant cells.We also performed a series of cell experiments confirming that GPX4 knockdown inhibited proliferation and induced cellular senescence,as determined by using CCK-8,colony formation,and EdU assay.In addition,SA-β-gal staining and senescence-associated secretory phenotype(SASP)components,such as P21 and Interleukin-6(IL-6),were increased in GPX4 knockdown cells,while Lamin B1 was decreased.Moreover,we predicted that high expression of GPX4 was related to low immune cell infiltration.Conclusion:This study demonstrates that GPX4 is a potential prognostic biomarker and target gene for COAD.展开更多
以高抗黄曲霉品种J11和高感品种金花1012黄曲霉处理的三个不同时期为材料,利用荧光定量PCR对种皮PnLOX2基因进行相对定量。结果表明:Real time PCR作为一种简单有效的定量方法,可快速检测待测基因的表达量差异;高抗黄曲霉品种J11在黄曲...以高抗黄曲霉品种J11和高感品种金花1012黄曲霉处理的三个不同时期为材料,利用荧光定量PCR对种皮PnLOX2基因进行相对定量。结果表明:Real time PCR作为一种简单有效的定量方法,可快速检测待测基因的表达量差异;高抗黄曲霉品种J11在黄曲霉侵染过程中PnLOX2基因表达量变化显著,而金花1012的表达量变化较小,表明PnLOX2基因在花生种皮中存在并且可能与抗黄曲霉相关。展开更多
Background:Colon adenocarcinoma(COAD)is the second leading cause of cancer death worldwide thus,identification of COAD biomarkers is critical.Mitotic Arrest Deficient 2 Like 2(MAD2L2)is a key factor in mammalian DNA d...Background:Colon adenocarcinoma(COAD)is the second leading cause of cancer death worldwide thus,identification of COAD biomarkers is critical.Mitotic Arrest Deficient 2 Like 2(MAD2L2)is a key factor in mammalian DNA damage repair and is highly expressed in many malignant tumors.This is a comprehensive study of MAD2L2 expression,its diagnostic value,prognostic analysis,potential biological function,and impact on the immune system of patients with COAD.Methods:Gene expression,clinical relevance,prognostic analysis,diagnostic value,GO/KEGG cluster analysis,data obtained from TCGA,and bioinformatics statistical analysis were performed using the R package.Immune responses to MAD2L2 expression in COAD were analyzed using TIMER.The expression of MAD2L2 in HCT116 cells induced by the inflammatory factor TNF-αwas detected using Western blot.Results:Our results underscore the clinical diagnostic value and potential biological significance of MAD2L2 in patients with COAD.A high level of MAD2L2 expression has been found in COAD and correlated with tumor status and colon polyps.ROC curve analysis showed that MAD2L2 expression has high diagnostic value in COAD.Analysis of immune infiltration results showed that MAD2L2 expression was positively correlated with neutrophil levels.The western blot results demonstrated that MAD2L2 was dose-dependently present with TNF-α.GO/KEGG revealed that MAD2L2 overexpressed and coexpressed genes were mostly involved in biological functions,including hypoxia response,response to reduced oxygen levels,mitochondrial translation elongation,and other processes.Conclusion:MAD2L2 as a new COAD biomarker contributes to our understanding of how alterations in gene expression and the immunological environment contribute to the development of colon cancer.Following further investigation,MAD2L2 may prove to be a viable target factor for clinical diagnosis and therapy of COAD.展开更多
文摘Background:Colorectal adenocarcinoma(COAD)is one of the most common gastrointestinal malignancies.There is a pressing need to recognize reliable biomarkers that can improve diagnostic accuracy,predict prognosis,and serve as effective molecular targets.Glutathione peroxidase 4(GPX4)is an important antioxidant protein.Evidence demonstrates that abnormal expression of GPX4 is related to cancer initiation and progression.However,the role of GPX4 in COAD remains unclear.Methods:We employed bioinformatics analysis and conducted subsequent validation of biological processes,including cell counting kit-8 assay(CCK-8),colony formation assay,reverse transcription-quantitative polymerase chain reaction(RT-qPCR),5-ethynyl-2′-deoxyuridine assay(EdU),western blot,immunohistochemistry,senescence associatedβ-galactosidase(SA-β-gal)staining and immunofluorescence to explore the expression status,prognostic value and biological function of GPX4 in COAD.Results:Our data revealed that GPX4 mRNA expression was upregulated in COAD tissues and could predict the prognosis in patients with COAD.High GPX4 expression was associated with increased infiltration of malignant cells.We also performed a series of cell experiments confirming that GPX4 knockdown inhibited proliferation and induced cellular senescence,as determined by using CCK-8,colony formation,and EdU assay.In addition,SA-β-gal staining and senescence-associated secretory phenotype(SASP)components,such as P21 and Interleukin-6(IL-6),were increased in GPX4 knockdown cells,while Lamin B1 was decreased.Moreover,we predicted that high expression of GPX4 was related to low immune cell infiltration.Conclusion:This study demonstrates that GPX4 is a potential prognostic biomarker and target gene for COAD.
文摘以高抗黄曲霉品种J11和高感品种金花1012黄曲霉处理的三个不同时期为材料,利用荧光定量PCR对种皮PnLOX2基因进行相对定量。结果表明:Real time PCR作为一种简单有效的定量方法,可快速检测待测基因的表达量差异;高抗黄曲霉品种J11在黄曲霉侵染过程中PnLOX2基因表达量变化显著,而金花1012的表达量变化较小,表明PnLOX2基因在花生种皮中存在并且可能与抗黄曲霉相关。
基金supported by the Ningxia Hui Autonomous Region Key Research and Development Program(Grant No.2021BEG03084).
文摘Background:Colon adenocarcinoma(COAD)is the second leading cause of cancer death worldwide thus,identification of COAD biomarkers is critical.Mitotic Arrest Deficient 2 Like 2(MAD2L2)is a key factor in mammalian DNA damage repair and is highly expressed in many malignant tumors.This is a comprehensive study of MAD2L2 expression,its diagnostic value,prognostic analysis,potential biological function,and impact on the immune system of patients with COAD.Methods:Gene expression,clinical relevance,prognostic analysis,diagnostic value,GO/KEGG cluster analysis,data obtained from TCGA,and bioinformatics statistical analysis were performed using the R package.Immune responses to MAD2L2 expression in COAD were analyzed using TIMER.The expression of MAD2L2 in HCT116 cells induced by the inflammatory factor TNF-αwas detected using Western blot.Results:Our results underscore the clinical diagnostic value and potential biological significance of MAD2L2 in patients with COAD.A high level of MAD2L2 expression has been found in COAD and correlated with tumor status and colon polyps.ROC curve analysis showed that MAD2L2 expression has high diagnostic value in COAD.Analysis of immune infiltration results showed that MAD2L2 expression was positively correlated with neutrophil levels.The western blot results demonstrated that MAD2L2 was dose-dependently present with TNF-α.GO/KEGG revealed that MAD2L2 overexpressed and coexpressed genes were mostly involved in biological functions,including hypoxia response,response to reduced oxygen levels,mitochondrial translation elongation,and other processes.Conclusion:MAD2L2 as a new COAD biomarker contributes to our understanding of how alterations in gene expression and the immunological environment contribute to the development of colon cancer.Following further investigation,MAD2L2 may prove to be a viable target factor for clinical diagnosis and therapy of COAD.
