The extensive use of cobalt nanoparticles(CoNPs)in industrial and biomedical applications has raised envi-ronmental concerns,necessitating effective mitigation strategies.This study examines the phytotoxicity and phyt...The extensive use of cobalt nanoparticles(CoNPs)in industrial and biomedical applications has raised envi-ronmental concerns,necessitating effective mitigation strategies.This study examines the phytotoxicity and phytoremediation potential of CoNPs using Lemna minor L.,a recognized Co-hyperaccumulator.CoNPs exhibited significant sublethal toxicity at concentrations≥1000μg Co^(2+)/L,leading to oxidative stress and impaired growth,photosynthesis,and respiration.Despite these challenges,L.minor effectively removed over 99%of CoNPs from the medium,even at high concentrations(up to 20.000μg Co^(2+)/L),with gravimetric cobalt con-centrations reaching 1771μg Co^(2+)/g dry weight in plant tissues.Physiological responses to CoNPs were similar to those induced by equivalent concentrations of CoCl_(2),suggesting that CoNPs dissolve into Co^(2+)ions upon interaction with plants.However,ultrastructural analysis revealed distinct intracellular cobalt localization with CoNPs causing more severe chloroplast damage than ionic Co^(2+).These findings suggest two toxicity mechanisms:ionic effects from dissolved Co^(2+)and direct physical damage from intact nanoparticles.The resilience and hyperaccumulation capacity of L.minor makes it a promising candidate for the phytoremediation of cobalt-contaminated waters.The study underscores the importance of further research to elucidate CoNP environ-mental behavior and optimize phytoremediation approaches.展开更多
基金financial support from several institutions,and we express our gratitude for their contributionsWe acknowledge the Coordenaçao de Aperfeiçoamento de Pessoal de Nível Superior of Brazil(CAPES,Finance Code 001)+1 种基金the Fundaçao Araucaria de Apoio ao Desenvolvimento Científico e Tecnologico do Parana,mainly through the Programa Paranaense de Pesquisas em Saneamento Ambiental(PPPSA/Sanepar,Finance Code SAN2021251000002)the Natural Science and Engineering Research Council of Canada(NSERC)(Finance Code RGPIN-2023-05681).
文摘The extensive use of cobalt nanoparticles(CoNPs)in industrial and biomedical applications has raised envi-ronmental concerns,necessitating effective mitigation strategies.This study examines the phytotoxicity and phytoremediation potential of CoNPs using Lemna minor L.,a recognized Co-hyperaccumulator.CoNPs exhibited significant sublethal toxicity at concentrations≥1000μg Co^(2+)/L,leading to oxidative stress and impaired growth,photosynthesis,and respiration.Despite these challenges,L.minor effectively removed over 99%of CoNPs from the medium,even at high concentrations(up to 20.000μg Co^(2+)/L),with gravimetric cobalt con-centrations reaching 1771μg Co^(2+)/g dry weight in plant tissues.Physiological responses to CoNPs were similar to those induced by equivalent concentrations of CoCl_(2),suggesting that CoNPs dissolve into Co^(2+)ions upon interaction with plants.However,ultrastructural analysis revealed distinct intracellular cobalt localization with CoNPs causing more severe chloroplast damage than ionic Co^(2+).These findings suggest two toxicity mechanisms:ionic effects from dissolved Co^(2+)and direct physical damage from intact nanoparticles.The resilience and hyperaccumulation capacity of L.minor makes it a promising candidate for the phytoremediation of cobalt-contaminated waters.The study underscores the importance of further research to elucidate CoNP environ-mental behavior and optimize phytoremediation approaches.
