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基于QRF-SA-ConvLSTM模型的网约车需求预测方法
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作者 李晓梅 吕斌 《交通信息与安全》 北大核心 2025年第2期127-135,共9页
网约车需求预测中考虑时空特征和不确定性影响对于提升交通系统的效率和稳定性至关重要。研究了融合分位数回归森林(quantile regression forest, QRF)和自注意力卷积长短期记忆网络(self-attention convolutional long short-term memo... 网约车需求预测中考虑时空特征和不确定性影响对于提升交通系统的效率和稳定性至关重要。研究了融合分位数回归森林(quantile regression forest, QRF)和自注意力卷积长短期记忆网络(self-attention convolutional long short-term memory, SA-ConvLSTM)的预测模型,通过协同优化时空特征学习与概率分布建模,实现高精度需求预测与不确定性估计。首先,模型利用分位数回归森林生成指定概率区间的需求预测分布,并结合注意力机制对原始数据加权融合,构建时空概率矩阵;随后将该矩阵输入SA-ConvLSTM模块,由卷积结构提取局部空间模式,自注意力机制聚焦关键时空节点,LSTM捕捉长期时间依赖;最终通过多任务输出层同步优化点预测与区间估计。该方法同时结合了分位数回归的分布建模能力与深度学习的时空特征提取优势,进而提升预测精度和不确定性量化能力。研究采用滴滴开源的西安市二环内网约车轨迹数据集验证模型的有效性和准确性。结果表明:均值预测中,提出模型的平均绝对误差和均方误差分别较SA-ConvLSTM模型降低了21%和17.2%;不确定性估计中,提出模型在95%置信度下的区间覆盖率相对于线性分位数回归(linear quantile regression,LQR)、QRF和SA-ConvLSTM模型分别提高了5.3%、2.5%、0.9%,同时区间平均宽度分别减少了41.5%、30%、18.5%。以上结论验证了模型的预测精度和可靠性。 展开更多
关键词 智能交通 网约车需求预测 QRF-SA-convLSTM 不确定性估计
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The ConMan Trial: Coagulase Negative Staphylococcal Infections: To Treat or Not to Treat
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作者 David Levine Henry Spratt +5 位作者 Caitlin Crews-Stowe Ryan Mart Jarle Stone Lani Gao Michael Davis Lindsey Brunton 《Open Journal of Blood Diseases》 2024年第4期91-100,共10页
Background: Coagulase negative Staphylococci (CoNS) positive blood cultures represent a complex and common challenge in clinical medicine. CoNS are common in skin flora and are often mistaken as contamination. Convers... Background: Coagulase negative Staphylococci (CoNS) positive blood cultures represent a complex and common challenge in clinical medicine. CoNS are common in skin flora and are often mistaken as contamination. Conversely, CoNS can also be implicated in severe and life-threatening bacteremia requiring prompt treatment. A gray area in terms of treatment approach exists for health care providers. The primary aim of this study was to examine predictive factors in patients who have positive CoNS blood cultures that met the CDC case definition of a laboratory confirmed bloodstream infection (LCBI-II) that were subsequently found to have clinically significant true bacteremia. Methods: A retrospective cohort of 288 patients that had at least two separate blood cultures positive for a coagulase negative Staphylococci between November 1st, 2017, and November 1st, 2018, were examined to determine if there were any patient specific factors that would indicate a true bacteremia with CoNS. Results: Retrospective regression analysis demonstrated that those subjected to antibiotics for two days or more (OR = 3.01), those subjected to antibiotics for seven days or more (OR = 2.86), and patients with central access (OR = 4.06) were more likely to have a true infection. Although not statistically significant, an association was also found in immunosuppressed patients (OR = 1.65), and in patients with implanted hardware (OR = 1.16). Conclusion: Patients receiving antibiotics for greater than two days and patients with a central line were more likely to have a true bloodstream infection with coagulase negative Staphylococci. 展开更多
关键词 Coagulase Negative Staphylococci (conS) Positive Blood Culture True Bacterium Central Access IMMUNOSUPPRESSED Infectious Disease
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Indirect Output Voltage Control in Negative Output Elementary Super Lift Luo Converter Using PIC plus FLC in Discontinuous Conduction Mode
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作者 S. Muthukaruppasamy A. Abudhahir 《Circuits and Systems》 2016年第11期3685-3704,共20页
In this paper, the design of a proportional integral controller (PIC) plus fuzzy logic controller (FLC) for the negative output elementary super lift Luo converter (NOESLLC) operated in discontinuous conduction mode (... In this paper, the design of a proportional integral controller (PIC) plus fuzzy logic controller (FLC) for the negative output elementary super lift Luo converter (NOESLLC) operated in discontinuous conduction mode (DCM) is presented. In spite of the many benefits viz. the high voltage transfer gain, the high efficiency, and the reduced inductor current and the capacitor voltage ripples, it natured with non-minimum phase. This characteristic makes the control of NOESLLC cumbersome. Any attempt of direct controlling the output voltage may erupt to instability. To overcome this problem, indirect regulation of the output voltage based on the two-loop controller is devised. The savvy in the inductor current control improves the dynamic response of the output voltage. The FLC is designed for the outer (voltage) loop while the inner (current) loop is controlled by the PIC. For the developed ?19.6 V NOESLLC, the dynamic performances for different perturbations (line, load and component variations) are obtained for PIC plus FLC and compared with PIC plus PIC. The study of two cases is performed at various operating regions by developing the MATLAB/Simulink model. 展开更多
关键词 Negative Output Elementary Super Lift Luo converter (NOESLLC) Discontinuous conduction Mode (DCM) Fuzzy Logic controller (FLC) Proportional-Integral con-troller (PIC)
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Assessment of Several Moist Adiabatic Processes Associated with Convective Energy Calculation 被引量:7
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作者 李耀东 高守亭 刘健文 《Advances in Atmospheric Sciences》 SCIE CAS CSCD 2004年第6期941-950,共10页
Several methods dealing with the moist adiabatic process are described in this paper. They are based on static energy conservation, pseudo-equivalent potential temperature conservation, the strict pseudo- adiabati... Several methods dealing with the moist adiabatic process are described in this paper. They are based on static energy conservation, pseudo-equivalent potential temperature conservation, the strict pseudo- adiabatic equation, and the reversible moist adiabatic process, respectively. Convective energy parame- ters, which are closely related to the moist adiabatic process and which re?ect the gravitational e?ects of condensed liquid water, are reintroduced or de?ned, including MCAPE [Modi?ed-CAPE (convective avail- able potential energy)], DCAPE (Downdraft-CAPE), and MDCAPE (Modi?ed-Downdraft-CAPE). Two real case analyses with special attention given to condensed liquid water show that the selection of moist adiabatic process does a?ect the calculated results of CAPE and the gravitational e?ects of condensed liq- uid water are not negligible in severe storms. Intercomparisons of these methods show that static energy conservation is consistent with pseudo-equivalent potential temperature conservation not only in physical properties but also in calculated results, and both are good approximations to the strict pseudo-adiabatic equation. The lapse rate linked with the reversible moist adiabatic process is relatively smaller than that linked with other moist adiabatic processes, especially when considering solidi?cation of liquid water in the reversible adiabatic process. 展开更多
关键词 moist adiabatic processes modified convective available potential energy downdraft con-vective available potential energy modified downdraft convective available potential energy reversible moist adiabatic process liquid water
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EXACT CONTROLLABILITY FOR FIRST ORDER QUASILINEAR HYPERBOLIC SYSTEMS WITH VERTICAL CHARACTERISTICS 被引量:1
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作者 李大潜 饶伯鹏 《Acta Mathematica Scientia》 SCIE CSCD 2009年第4期980-990,共11页
We consider first order quasilinear hyperbolic systems with vertical characteristics. It was shown in [4] that such systems can be exactly controllable with the help of internal controls applied to the equations corr... We consider first order quasilinear hyperbolic systems with vertical characteristics. It was shown in [4] that such systems can be exactly controllable with the help of internal controls applied to the equations corresponding to zero eigenvalues. However, it is possible that, for physical or engineering reasons, we can not put any control on the equations corresponding to zero eigenvalues. In this paper, we will establish the exact controllability only by means of physically meaningfnl internal controls applied to the equations corresponding to non-zero eigenvalues. We also show the exact controllability for a very simplified model by means of switching controls. 展开更多
关键词 quasilinear hyperbolic systems exact controllability local distributed con-trol switching controls
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Constrained cooperative control design for distributed morphing wing systems
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作者 Zhen He Yuping Lu 《Journal of Systems Engineering and Electronics》 SCIE EI CSCD 2012年第4期588-595,共8页
An efficient design method is proposed for the cooperative control problem of morphing wing systems with distributed structures and bounded control inputs. The multi-agent model of the distributed morphing wing system... An efficient design method is proposed for the cooperative control problem of morphing wing systems with distributed structures and bounded control inputs. The multi-agent model of the distributed morphing wing system is established. The cooperative controllers with saturation constraints are presented. By introducing the concepts in consensus algorithms, the cooperative information links in the controllers are described by graphs, and the corresponding Laplacian matrix is defined. The design conditions of the cooperative controllers are proposed, in the form of linear matrix inequalities. For the case of undirected information links, the controller design conditions are simplified as algebraic inequalities, which highly reduce the computation cost. The designed controllers are implemented on a distributed morphing wing platform, and experiments are carried out. Simulation and experiment results show that the controllers can make all the actuating units in the morphing wing system cooperatively achieve the desired positions, which demonstrates the effectiveness of the proposed theory. 展开更多
关键词 morphing wing cooperative control saturation con-straints consensus algorithm.
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3D reconstruction method based on contour features
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作者 HAN Bao-ling ZHU Ying +2 位作者 LUO Qing-sheng XU Bo ZHANG Tian 《Journal of Beijing Institute of Technology》 EI CAS 2016年第3期301-308,共8页
To guarantee the accuracy and real-time of the 3D reconstruction method for outdoor scene,an algorithm based on region segmentation and matching was proposed.Firstly,on the basis of morphological gradient information,... To guarantee the accuracy and real-time of the 3D reconstruction method for outdoor scene,an algorithm based on region segmentation and matching was proposed.Firstly,on the basis of morphological gradient information,obtained by comparing color weight gradient images and proposing a multi-threshold segmentation,scene contour features were extracted by a watershed algorithm and a fuzzy c-means clustering algorithm.Secondly,to reduce the search area,increase the correct matching ratio and accelerate the matching speed,the region constraint was established according to a region's local position,area and gray characteristics,the edge pixel constraint was established according to the epipolar constraint and the continuity constraint.Finally,by using the stereo matching edge pixel pairs,their 3D coordinates were estimated according to the binocular stereo vision imaging model.Experimental results show that the proposed method can yield a high stereo matching ratio and reconstruct a 3D scene quickly and efficiently. 展开更多
关键词 gradient map watershed algorithm fuzzy c-means clustering algorithm region con-straint contour matching 3D reconstruction
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青蒿琥酯对Con A诱导小鼠自身免疫性肝损伤的保护作用研究 被引量:12
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作者 曹婕 赵昕 +2 位作者 刘明江 郑卉 李金贵 《中国中药杂志》 CAS CSCD 北大核心 2018年第10期2123-2128,共6页
免疫性肝病是临床上难治性疾病,目前没有特效性药物,因此,研发新的有效干预药物,对防治免疫性肝病有重要的临床价值。为探讨青蒿琥酯(artesunate,Art)对免疫性肝损伤的保护作用,该研究采用不同质量浓度(27,54,108 mg·kg-1)Art... 免疫性肝病是临床上难治性疾病,目前没有特效性药物,因此,研发新的有效干预药物,对防治免疫性肝病有重要的临床价值。为探讨青蒿琥酯(artesunate,Art)对免疫性肝损伤的保护作用,该研究采用不同质量浓度(27,54,108 mg·kg-1)Art灌胃小鼠连续7 d,然后尾静脉注射伴刀豆球蛋白A(concanavalin A,Con A)诱导小鼠肝损伤模型。于造模后8 h采血液和肝组织进行血清转氨酶(ALT,AST)、组织病变、炎性细胞因子以及NF-κB关键蛋白表达水平的检测。结果表明,与模型组相比,Art高浓度组显著降低了模型小鼠的肝指数、ALT和AST水平(P〈0.01),组织病变也明显减轻,同时降低了促炎细胞因子TNF-α,IFN-γ,IL-6,IL-17的水平而增加了炎性抑制因子IL-10的表达(P〈0.05),并呈现明显的剂量-效应关系。Western blot法检测结果显示108 mg·kg-1Art可显著抑制NF-κB关键蛋白p-p65和p-IκBα的表达(P〈0.01)。NF-κB特异阻断剂吡咯烷二硫代氨基甲酸酯(PDTC)也可显著抑制p-p65和p-IκBα表达并减轻肝损伤。以上结果说明Art主要通过抑制NF-κB信号通路而对Con A诱导的小鼠免疫性肝损伤发挥保护作用。该研究为青蒿琥酯用于自身免疫性肝损伤的防治提供了参考。 展开更多
关键词 青蒿琥酯 conA诱导小鼠免疫性肝损伤 细胞因子 NF-ΚB
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Proton Conductivity of Ni, Y Co-Doped BaZrO<sub>3</sub>
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作者 Hiroya Morishita Yumiko Ikebe Eriko Ban 《Journal of Materials Science and Chemical Engineering》 2018年第6期19-27,共9页
Dense sintered bodies of proton conducting BaZrO3 (BZ) and Y-doped BaZrO3 (BZ-Y) were obtained at 1600℃ for a short sintering time of 5 hours, by the addition of NiO as a sintering promotion agent. The relative densi... Dense sintered bodies of proton conducting BaZrO3 (BZ) and Y-doped BaZrO3 (BZ-Y) were obtained at 1600℃ for a short sintering time of 5 hours, by the addition of NiO as a sintering promotion agent. The relative density and grain growth of samples, Ni-doped BaZrO3 (BZ-N) and Ni, Y co-doped BaZrO3 (BZ-NY), were increased with increasing Ni addition. The sinterability of BZ-NY was greatly improved just to add only 0.6 mol% Ni and the relative density of this sample was more than 98%, in contrast to that of 60% at most for BZ-Y without Ni addition. Electrical conductivity of BZ-NY added Ni 1.0 mol%, BaZr0.91Ni0.01Y0.08O3-α, was more than 10-3 S.cm-2 at 900℃?in a wet 1% hydrogen atmosphere, which value was 10 times higher than that of BZ-Y. In addition, the kind of electrical conduction carrier and an ionic transport number were also examined by employing various concentration cells. It was found that the proton conduction was dominant for both BZ-N and BZ-NY samples, although BZ-NY showed scarcely oxygenion conduction approximately 10% in a high temperature range higher than 800℃. From these results, as mall amount of Ni addition found to be effective for improvement of both the sinterability and the electrical conductivity. 展开更多
关键词 Proton conducting Oxide Barium ZIRconATE NI Y CO-DOPING Electrical con-ductivity Ionic Transport Number
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Rhn、Con(n=3~56)团簇的结构特性研究 被引量:9
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作者 张蓓 段海明 张军 《原子与分子物理学报》 CAS CSCD 北大核心 2007年第1期63-68,共6页
采用半经验的Gupta多体势结合遗传算法对Rhn、Con(n=3~56)团簇的基态结构特性进行了系统的研究.除在n=18~40尺寸范围内有少数团簇的构型不同,两种团簇具有相似的几何结构.在Rhn、Con(n=3~56)团簇的生长中,存在类Ih构型与类fcc构... 采用半经验的Gupta多体势结合遗传算法对Rhn、Con(n=3~56)团簇的基态结构特性进行了系统的研究.除在n=18~40尺寸范围内有少数团簇的构型不同,两种团簇具有相似的几何结构.在Rhn、Con(n=3~56)团簇的生长中,存在类Ih构型与类fcc构型之间的竞争,对于n≤24,两种团簇都从紧致密堆积结构过渡为类二十面体构型,Rh38及Co38为具有Oh对称性的类fcc构型,从n=39开始,铑团簇和钴团簇都呈现出明显的Ih生长模式.两种团簇的平均束缚能随原子数目的增加而增大,且在所研究的尺寸范围内铑团簇的平均结合能高于钴团簇.Rhn、Con(n=3~56)团簇具有相同的幻数序列:n=13,19,23,38,55. 展开更多
关键词 Rhn、con团簇 遗传算法 Gupta多体势
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白藜芦醇对小鼠ConA肝脏损伤的保护作用 被引量:4
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作者 夏玉敬 林春蕾 +3 位作者 周莹群 卢洁 戴维奇 郭传勇 《世界临床药物》 CAS 2014年第5期300-304,共5页
目的探讨白藜芦醇(resveratrol)对肝脏损伤的保护作用。方法 50只小鼠随机分成5组,每组10只,分别为:正常对照组、模型组、白藜芦醇低剂量组、白藜芦醇中剂量组、白藜芦醇高剂量组。实验前两周,白藜芦醇组各灌服相应的药物一日0.1 ml/10... 目的探讨白藜芦醇(resveratrol)对肝脏损伤的保护作用。方法 50只小鼠随机分成5组,每组10只,分别为:正常对照组、模型组、白藜芦醇低剂量组、白藜芦醇中剂量组、白藜芦醇高剂量组。实验前两周,白藜芦醇组各灌服相应的药物一日0.1 ml/10gBW。正常组和模型组则灌服0.5%羧甲基纤维素溶液一日0.1 ml/10gBW。第14天灌服结束后1小时,除正常组外各组动物均从尾静脉注射刀豆蛋白溶液一日0.05 ml/10gBW,正常对照组动物则尾静脉注射生理盐水一日0.05 ml/10gBW。测定ALT、AST;取肝右叶置于4%多聚甲醛4℃下固定,用于HE染色。结果肝脏肉眼观察及HE染色后病理观察均提示模型组肝脏损伤明显重于白藜芦醇组,且模型组血清ALT、AST水平与对照组相比升高,差别具有显著统计学意义(P<0.01)。白藜芦醇高、中、低剂量组血清ALT、AST水平均较模型组下降,差别具有显著统计学意义(P<0.01)。白藜芦醇各剂量组间的差异也无统计学意义。与正常组相比,不同浓度白藜芦醇治疗组中炎症因子IL-2、IL-6、TNF-α表达量增加,各浓度间无明显差异。与模型组相比,各浓度组中炎症因子表达量明显下降。结论白藜芦醇对刀豆蛋白诱导的急性肝损伤有明显的保护作用。 展开更多
关键词 白藜芦醇 肝损伤 保护作用 刀豆蛋白(con A)
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小鼠精子表面Con A结合糖复合物的形成与变化 被引量:5
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作者 王家鑫 孙秀华 +1 位作者 周占祥 邓泽沛 《中国组织化学与细胞化学杂志》 CAS CSCD 1996年第3期259-263,374,共6页
用辣根过氧化物酶标记的ConA(伴刀豆素A)对小鼠睾丸与附睾切片,以及对取自附睾和子宫(交配后)内的精子涂片进行了标记,旨在认识精子在发生、成熟和获能过程中表面糖复合物的形成与变化。本研究表明,睾丸内的生精细胞和支持... 用辣根过氧化物酶标记的ConA(伴刀豆素A)对小鼠睾丸与附睾切片,以及对取自附睾和子宫(交配后)内的精子涂片进行了标记,旨在认识精子在发生、成熟和获能过程中表面糖复合物的形成与变化。本研究表明,睾丸内的生精细胞和支持细胞均呈ConA标记阳性。附睾的输出小管和附睾管上皮细胞,ConA标记呈中度至强阳性,有部位的差别。附睾头和附睾尾内精子表面的标记无明显差别,标记位置均主要在顶体区和尾部。精子在子宫内存留1.5小时后,顶体后区出现中度阳性标记,但存留3小时和6小时后,顶体和顶体后区的标记均减弱或消失。这些结果提示,(1)精子发生期即可合成ConA结合糖复合物,(2)精子在附睾成熟过程中表面的ConA结合糖复合物无明显变化,(3)精子获能后顶体后区出现的ConA结合糖复合物可能与受精能力有关。 展开更多
关键词 conA标记 睾丸 附睾 精子成熟与获能 小鼠
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B7-H4对ConA诱导小鼠肝损伤保护作用的实验研究 被引量:2
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作者 肖欢 潘卫民 +2 位作者 王超群 陈鸿颜 徐军发 《四川大学学报(医学版)》 CAS CSCD 北大核心 2015年第6期842-845,共4页
目的探讨B7-H4对免疫性损伤肝脏的保护作用及其机制。方法将KM小鼠分为4组(每组15只):生理盐水组(A组)、pcDNA3.1-mB7-H4-Fc组(B组)、pcDNA3.1组(C组)和刀豆球蛋白A(Con A)组(D组)。B组、C组和D组动物均通过尾静脉注射Con A 25mg/kg制... 目的探讨B7-H4对免疫性损伤肝脏的保护作用及其机制。方法将KM小鼠分为4组(每组15只):生理盐水组(A组)、pcDNA3.1-mB7-H4-Fc组(B组)、pcDNA3.1组(C组)和刀豆球蛋白A(Con A)组(D组)。B组、C组和D组动物均通过尾静脉注射Con A 25mg/kg制备肝损伤模型。在Con A注射前1d,分别给予B组每只小鼠注射pcDNA3.1-mB7-H4-Fc 100μg和C组每只小鼠注射pcDNA3.1 100μg。并于注射Con A后12h、24h和48h,各组取5只小鼠,摘眼球取血,分离血清行白介素-4(IL-4)、γ-干扰素(IFN-γ)、谷丙转氨酶(ALT)和谷草转氨酶(AST)浓度的测定;引颈处死小鼠后取肝脏,用于组织病理学检测。结果小鼠血清IL-4、IFN-γ、ALT和AST水平在注射Con A后各时点,B、C和D三组较A组均有升高(P<0.05),但B组较C组和D组减低,B组与C组间差异有统计学意义(P<0.01)。小鼠肝组织切片经HE染色,光学显微镜下可见,B组小鼠肝脏损伤均比C组和D组有不同程度的减轻。结论初步证实了B7-H4对Con A诱导的肝脏免疫性损伤具有保护作用。B7-H4可能是通过抑制IL-4和IFN-γ的产生和/或分泌而发挥对肝脏保护的作用。 展开更多
关键词 B7-H4 con A-诱导性肝损伤 保护作用 机制
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加味当归芍药散对Con A诱导的免疫性肝损伤小鼠保护作用 被引量:7
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作者 张驰 魏琳 +1 位作者 王军 宋伍 《中药药理与临床》 CAS CSCD 北大核心 2019年第6期11-15,共5页
目的:考察加味当归芍药散对刀豆蛋白A(Con A)诱导的免疫性肝损伤小鼠的保护作用,并探讨其作用机制。方法:将30只BALB/c雄性小鼠随机分为5组:空白对照组、Con A模型对照组、联苯双酯0.2 g/kg组、当归芍药散5 g/kg、15 g/kg组。每组6只。... 目的:考察加味当归芍药散对刀豆蛋白A(Con A)诱导的免疫性肝损伤小鼠的保护作用,并探讨其作用机制。方法:将30只BALB/c雄性小鼠随机分为5组:空白对照组、Con A模型对照组、联苯双酯0.2 g/kg组、当归芍药散5 g/kg、15 g/kg组。每组6只。灌胃给予相应药物或生理盐水,连续7日,末次药后1 h,采用Con A制备急性肝损伤模型,造模8 h后取材。采用试剂盒检测肝脏组织匀浆液SOD及血清ALT、AST的活力;ELISA法检测肝脏匀浆液MDA及血清NO、TNF-α、IFN-γ、IL-4及IL-6的含量;HE染色法观察肝脏组织病理学的改变;应用Western Blot法检测肝脏组织中Nrf2、Keap1蛋白的表达情况。结果:与空白对照组比较,Con A模型对照组小鼠肝脏匀浆液中MDA含量升高,而SOD活性降低,血清中ALT、AST、NO、TNF-α、IFN-γ、IL-4及IL-6水平显著升高。加味当归芍药散15 g/kg能显著降低肝脏匀浆液MDA含量及增强SOD活性,加味当归芍药散5、15 g/kg均可抑制血清中ALT、AST、NO、TNF-α、IFN-γ、IL-4及IL-6含量的升高,明显上调Nrf2、Keap1蛋白的表达。结论:加味当归芍药散对Con A诱导急性小鼠肝损伤具有明显的保护作用,其机制可能与上调Nrf2信号通路有关。 展开更多
关键词 con A 加味当归芍药散 免疫性肝损伤 氧化损伤 Nrf2 KEAP1
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卵母细胞骨架与染色体行为及质膜表面ConA结合位点关系的研究 被引量:3
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作者 刘辉 陈大元 《解剖学报》 CAS CSCD 北大核心 1996年第1期42-47,共6页
用体外培养和荧光双重标记技术研究小鼠卵母细胞骨架、染色体及细胞质膜表面(微绒毛,ConA结合位点)三者间相互关系。结果表明,不同的细胞骨架成分对染色体行为及质膜表面变化影响不同,中期微管主要聚合成纺锤体,构成减数分裂... 用体外培养和荧光双重标记技术研究小鼠卵母细胞骨架、染色体及细胞质膜表面(微绒毛,ConA结合位点)三者间相互关系。结果表明,不同的细胞骨架成分对染色体行为及质膜表面变化影响不同,中期微管主要聚合成纺锤体,构成减数分裂器的支架,影响染色体在赤道板上有序地排列和分离;微丝在卵母细胞皮质区呈极性分布,它是牵引减数分裂器(或染色体团)从细胞中央移到细胞皮质区的关键成分,同时微丝也参与后期和末期成对染色体的分离和向减数分裂器两极迁移过程。靠近减数分裂器的细胞质膜表面变化(微绒毛及ConA结合位点缺失)与染色体团和微丝相关,与微管无关。另外,后期和末期靠近减数分裂器上中体的质膜表面微绒毛和ConA结合位点增加,同时皮质区微丝聚集;中体和质膜间的相互作用依赖微丝介导。 展开更多
关键词 染色体 conA 卵母细胞 细胞骨架 质膜
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注射用胡黄连总苷对ConA引起小鼠急性免疫性肝损伤的保护作用研究 被引量:4
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作者 王淑娟 贾志丹 +3 位作者 魏怀玲 苗露阳 张丹 孙华 《中国药物警戒》 2013年第12期705-708,共4页
目的利用Concanavalin A(ConA)引起的免疫性肝损伤模型,对注射用胡黄连总苷的保肝活性和量效关系进行研究。方法注射用胡黄连总苷静脉给予ICR小鼠(0.5~8mg·kg-1×5),于末次给药后2h,动物尾静脉ConA 20mg·kg-1,建立急性免... 目的利用Concanavalin A(ConA)引起的免疫性肝损伤模型,对注射用胡黄连总苷的保肝活性和量效关系进行研究。方法注射用胡黄连总苷静脉给予ICR小鼠(0.5~8mg·kg-1×5),于末次给药后2h,动物尾静脉ConA 20mg·kg-1,建立急性免疫性肝损伤模型,16h后处理动物,制备血清,全自动生化分析仪检测血清ALT,AST及LDH水平,H.E.染色考察肝脏病理状态。结果 ConA 20mg·kg-1能引起小鼠显著的急性免疫性肝损伤,血清ALT,AST及LDH含量均显著升高,肝组织出现以炎症细胞浸润、肝细胞坏死为主要特征的病理改变。注射用胡黄连总苷0.5~4mg·kg-1剂量对ConA引起的肝损伤有显著保护作用,明显降低血清转氨酶水平,改善肝脏病理状态,其中1mg·kg-1剂量药效最佳,8mg·kg-1剂量药效有所下降,但此剂量未显示明显毒性。结论注射用胡黄连总苷对ConA引起的免疫性肝损伤有明确的保护作用,其起效剂量低,高于8mg·kg-1活性下降,在临床实验时需注意剂量的选择问题。 展开更多
关键词 胡黄连 总苷 注射液 conA 肝损伤
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Con A、α-Galcer与LPS/D-Gal N诱导免疫性肝损伤小鼠NKT细胞功能改变的比较研究 被引量:1
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作者 游佳 陈薇 +2 位作者 郑琦 陈靖 朱月永 《中国应用生理学杂志》 CAS CSCD 北大核心 2018年第6期489-491,523,583,共5页
目的:通过比较三种免疫性肝损伤模型小鼠NKT细胞功能改变情况,寻找一种在病理生理机制上更接近临床特点的免疫性肝损伤动物模型。方法:40只小鼠随机分为空白对照组、Con A模型组、α-Galcer模型组、LPS/D-Gal N模型组,每组10只。Con A... 目的:通过比较三种免疫性肝损伤模型小鼠NKT细胞功能改变情况,寻找一种在病理生理机制上更接近临床特点的免疫性肝损伤动物模型。方法:40只小鼠随机分为空白对照组、Con A模型组、α-Galcer模型组、LPS/D-Gal N模型组,每组10只。Con A模型组尾静脉注射ConA溶液(18 mg/kg),α-Galcer模型组腹腔注射α-Galcer溶液(40μg/kg),LPS/D-Gal N模型组腹腔注射LPS溶液(10μg/kg)和D-Gal N溶液(700 mg/kg)。造模完成后8 h,检测小鼠血清转氨酶ALT、AST水平,计算肝指数,采用HE染色法观察肝组织病理改变情况,流式细胞仪分析肝组织NKT细胞含量,Western blot法检测肝组织中TNF-α、IFN-γ、IL-6蛋白表达情况。结果:与空白对照组比较,各模型组小鼠血清ALT、AST水平均显著升高(P<0.01),肝指数增加(P<0.01或P<0.05),肝组织病理损伤明显,NKT细胞含量显著增加(P<0.01或P<0.05),TNF-α、IFN-γ、IL-6表达均明显上调(P<0.01);各模型组之间比较,α-Galcer模型组血清ALT、AST含量显著低于Con A组(P<0.05),病理损伤也相对较轻,LPS/D-Gal N模型组NKT细胞含量明显低于其余两组(P<0.05)。结论:Con A、α-Galcer和LPS/D-Gal N诱导的免疫性肝损伤模型小鼠NKT细胞明显激活,介导炎症紊乱;其中,以Con A诱导的动物模型肝脏病理损伤及免疫紊乱最为明显,更符合疾病临床特点,可作为免疫性肝损伤的首选模型。 展开更多
关键词 免疫性肝损伤 小鼠 NKT细胞 con A Α-GALCER LPS/D-Gal N
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Wistar大鼠和RCS大鼠视网膜色素上皮细胞和视杆细胞外节表面Con-A受体 被引量:2
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作者 李红艳 朱秀安 +1 位作者 唐军民 唐岩 《中国组织化学与细胞化学杂志》 CAS CSCD 1995年第3期253-257,332-333,共7页
为探讨正常大鼠与吞噬功能缺陷大鼠视网膜色素上皮(RPE)和视杆细胞外节(ROS)膜表面的甘露糖受体的存在和分布,本实验用胶体金标记物对12只Wistar大鼠和12只RCS大鼠的RPE细胞及临近其顶部的ROS表面的Co... 为探讨正常大鼠与吞噬功能缺陷大鼠视网膜色素上皮(RPE)和视杆细胞外节(ROS)膜表面的甘露糖受体的存在和分布,本实验用胶体金标记物对12只Wistar大鼠和12只RCS大鼠的RPE细胞及临近其顶部的ROS表面的Con-A受体进行标记.结果表明。两种大鼠RPE细胞及ROS膜表面均有胶体金标记物.胶体金标记物在两种RPE细胞微绒毛上分布密度较低,而在两种大鼠ROS膜表面分布密度则高。说明两种大鼠RPE细胞和ROS膜表面均有Con-A受体分布。在两种大鼠ROS被RPE细胞吞入后,均无胶体金标记物。 展开更多
关键词 视网膜色素上皮 视杆细胞外节 con-A受体 胶体金标记物
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ConA不同给药途径所致C57小鼠急、慢性肝损伤模型的建立与比较 被引量:2
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作者 于慧杰 李红 +1 位作者 郭明星 盛国光 《湖北中医药大学学报》 2014年第4期5-7,共3页
目的用ConA通过尾静脉注射、腹腔注射两种给药途径对C57小鼠分别建立急、慢性肝损伤模型,观察ConA不同给药途径造模是否存在差异,并研究ConA造成肝损伤的作用机制。方法用6mg/kg的ConA分别通过尾静脉注射、腹腔注射方法干预C57小鼠,注... 目的用ConA通过尾静脉注射、腹腔注射两种给药途径对C57小鼠分别建立急、慢性肝损伤模型,观察ConA不同给药途径造模是否存在差异,并研究ConA造成肝损伤的作用机制。方法用6mg/kg的ConA分别通过尾静脉注射、腹腔注射方法干预C57小鼠,注射8h后处死小鼠建立急性肝损伤模型;用6mg/kg的ConA每周注射1次,连续4周,建立慢性肝损伤模型,观察两种给药途径能否成功建立慢性肝损伤模型,及造成的慢性肝损伤程度有无差异。结果在ConA用量为6mg/kg时通过尾静脉注射能成功的建立急、慢肝损伤模型。而用腹腔注射给药建立急、慢性肝损伤模型存在结果不稳定性,不能成功建立肝损伤模型。结论给药途径对于ConA的药效发挥存在影响,尾静脉注射造成的肝损伤更为严重。 展开更多
关键词 conA 急性肝损伤 慢性肝损伤 C57BL/6J小鼠
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ConA、LPS对小鼠肺、脾和胸腺细胞体外分泌CSF的影响 被引量:2
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作者 张鸿来 刘树铮 《白求恩医科大学学报》 CSCD 1992年第4期309-311,共3页
现已证明,机体除骨髓基质细胞之外,其它的细胞也能分泌造血细胞集落刺激因子(CSF)。实验研究发现,体外培养的小鼠肺、脾及胸腺细胞在受到ConA或LPS的刺激作用后,CSF分泌量明显增加,在低剂量时(1.0μg/孔),即有促进CSF分泌的作用,在5.0... 现已证明,机体除骨髓基质细胞之外,其它的细胞也能分泌造血细胞集落刺激因子(CSF)。实验研究发现,体外培养的小鼠肺、脾及胸腺细胞在受到ConA或LPS的刺激作用后,CSF分泌量明显增加,在低剂量时(1.0μg/孔),即有促进CSF分泌的作用,在5.0μg/孔时,促进CSF分泌的作用达最高水平。结果表明,体内许多细胞受到丝裂原刺激时,CSF分泌量增加,有利于激活造血和免疫系统,增强机体的抵抗力。 展开更多
关键词 造血细胞集落 刺激因子 conA LPS
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