Background:Terpinen-4-ol(T4O),a key constituent of tea tree essential oil and various aromatic plants,has shown promising antiproliferative and pro-apoptotic effects in melanoma and other cancer types.However,its effi...Background:Terpinen-4-ol(T4O),a key constituent of tea tree essential oil and various aromatic plants,has shown promising antiproliferative and pro-apoptotic effects in melanoma and other cancer types.However,its efficacy against cutaneous squamous cell carcinoma(cSCC)remains unclear.Thus,in this study,we investigated the in vivo and in vitro effects of T4O on cSCC cell lines and preliminarily explored its impacting pathways.Methods:Using CCK8 and assay colony formation,we assessed the viability of cSCC A431,SCL-1,and COLO-16 cells treated with T40 at varying concentrations(0,1,2,and 4μM).Flow cytometry was employed to evaluate T4O’s effect on cSCC cell’s cycle progression and apoptosis induction.Additionally,western blotting was utilized to examine the expression intensities of N-cadherin and E-cadherin,two indicative markers of the epithelial-mesenchymal transition(EMT)pathway.T4O’s in vivo effect on inhibiting tumor progression was evaluated on an established xenograft tumor model.Then,the molecular mechanisms of T4O’s antitumor effect were explored by an integrated genome-wide transcriptomics and proteomics study on cSCC A431c cells.Finally,calpain-2’s potential mediator role in T4O’s anti-tumor mechanism was investigated in calpain-2 knockdown cell lines prepared via siRNA transfection.Result:It’s demonstrated that T4O treatment inhibited cSCC proliferation,clonogenicity,migration,and invasion while inducing apoptosis and suppressing the EMT pathway.T4O administration also inhibited cSCC tumorigenesis in the xenograft tumor model.RNA-sequencing and iTRAQ analysis detected significant upregulation of calpain-2 expression in T4O-treated cSCC cells.Western blotting confirmed that T4O significantly increased calpain-2 expression and promoted proteolytic cleavage ofβ-catenin and caspase-12,two calpain-2 target proteins.Importantly,siRNA-mediated calpain-2 knockdown relieved T4O’s suppressive effect on cSCC cell proliferation and motility.Mechanistically,T4O upregulates calpain-2 expression and promotes the cleavage ofβ-catenin and caspase-12,with siRNA-mediated calpain-2 knockdown mitigating T4O’s suppressive effects.Conclusion:These findings suggest that T4O’s antitumor activity in cSCC is mediated through the upregulation of calpain-2 expression and subsequent modulation ofβ-catenin and caspase-12.展开更多
为评估晒田期不同水分管理对水稻田温室气体排放影响,2023−2024年在辽宁省沈阳市开展田间试验,在水稻分蘖末期晒田时设置3种水分管理处理,其中,S1处理为晒田期正常灌溉,S2处理为晒田期不灌溉,S3处理为晒田期间隔灌溉。结果表明:①CH_(4...为评估晒田期不同水分管理对水稻田温室气体排放影响,2023−2024年在辽宁省沈阳市开展田间试验,在水稻分蘖末期晒田时设置3种水分管理处理,其中,S1处理为晒田期正常灌溉,S2处理为晒田期不灌溉,S3处理为晒田期间隔灌溉。结果表明:①CH_(4)排放通量呈明显的季节性变化特征,主要集中在分蘖至拔节孕穗期;N_(2)O排放通量则呈现多峰特征,排放高峰多出现在灌浆期。②不同水分管理下CH_(4)和N_(2)O排放通量与土壤理化性质的相关性呈明显差异。S1处理维持强还原环境,增加了CH_(4)排放但抑制N_(2)O排放;S2处理降低了土壤含水量,降低CH_(4)排放的同时增加了N_(2)O排放;S3处理因干湿交替频繁N_(2)O排放通呈现剧烈波动。在2023年S1处理中,CH_(4)排放通量与土壤温度呈显著正相关(P<0.01);S2处理CH_(4)排放通量与土壤孔隙空气相对湿度呈显著正相关(P<0.01),与土壤温度的相关性(P<0.05)次之。S1与S3处理的N_(2)O排放通量均与土壤温度呈显著相关(P均小于0.05),2024年二者相关性发生明显变化,其中S1处理中CH_(4)排放通量与土壤温度相关性减弱,但与pH呈显著负相关(P<0.01)。③不同处理间温室气体综合排放强度(greenhouse gas intensity,GHGI)存在显著差异(P均小于0.05),S2处理的GHGI在2023年与2024年试验中均最低,在维持产量的同时具有减排优势。研究显示,稻田CH_(4)与N_(2)O排放呈此消彼长关系,水分管理为核心调控因素,优化水分调控策略(如精准干湿交替)可实现协同减排。展开更多
基金supported by the Basic Research Program of the Guizhou Science Cooperation Foundation Project(Grant Number:ZK[2021]466)Guizhou Provincial Health Commission(Grant Number:gzwkj2022-062).
文摘Background:Terpinen-4-ol(T4O),a key constituent of tea tree essential oil and various aromatic plants,has shown promising antiproliferative and pro-apoptotic effects in melanoma and other cancer types.However,its efficacy against cutaneous squamous cell carcinoma(cSCC)remains unclear.Thus,in this study,we investigated the in vivo and in vitro effects of T4O on cSCC cell lines and preliminarily explored its impacting pathways.Methods:Using CCK8 and assay colony formation,we assessed the viability of cSCC A431,SCL-1,and COLO-16 cells treated with T40 at varying concentrations(0,1,2,and 4μM).Flow cytometry was employed to evaluate T4O’s effect on cSCC cell’s cycle progression and apoptosis induction.Additionally,western blotting was utilized to examine the expression intensities of N-cadherin and E-cadherin,two indicative markers of the epithelial-mesenchymal transition(EMT)pathway.T4O’s in vivo effect on inhibiting tumor progression was evaluated on an established xenograft tumor model.Then,the molecular mechanisms of T4O’s antitumor effect were explored by an integrated genome-wide transcriptomics and proteomics study on cSCC A431c cells.Finally,calpain-2’s potential mediator role in T4O’s anti-tumor mechanism was investigated in calpain-2 knockdown cell lines prepared via siRNA transfection.Result:It’s demonstrated that T4O treatment inhibited cSCC proliferation,clonogenicity,migration,and invasion while inducing apoptosis and suppressing the EMT pathway.T4O administration also inhibited cSCC tumorigenesis in the xenograft tumor model.RNA-sequencing and iTRAQ analysis detected significant upregulation of calpain-2 expression in T4O-treated cSCC cells.Western blotting confirmed that T4O significantly increased calpain-2 expression and promoted proteolytic cleavage ofβ-catenin and caspase-12,two calpain-2 target proteins.Importantly,siRNA-mediated calpain-2 knockdown relieved T4O’s suppressive effect on cSCC cell proliferation and motility.Mechanistically,T4O upregulates calpain-2 expression and promotes the cleavage ofβ-catenin and caspase-12,with siRNA-mediated calpain-2 knockdown mitigating T4O’s suppressive effects.Conclusion:These findings suggest that T4O’s antitumor activity in cSCC is mediated through the upregulation of calpain-2 expression and subsequent modulation ofβ-catenin and caspase-12.
文摘为评估晒田期不同水分管理对水稻田温室气体排放影响,2023−2024年在辽宁省沈阳市开展田间试验,在水稻分蘖末期晒田时设置3种水分管理处理,其中,S1处理为晒田期正常灌溉,S2处理为晒田期不灌溉,S3处理为晒田期间隔灌溉。结果表明:①CH_(4)排放通量呈明显的季节性变化特征,主要集中在分蘖至拔节孕穗期;N_(2)O排放通量则呈现多峰特征,排放高峰多出现在灌浆期。②不同水分管理下CH_(4)和N_(2)O排放通量与土壤理化性质的相关性呈明显差异。S1处理维持强还原环境,增加了CH_(4)排放但抑制N_(2)O排放;S2处理降低了土壤含水量,降低CH_(4)排放的同时增加了N_(2)O排放;S3处理因干湿交替频繁N_(2)O排放通呈现剧烈波动。在2023年S1处理中,CH_(4)排放通量与土壤温度呈显著正相关(P<0.01);S2处理CH_(4)排放通量与土壤孔隙空气相对湿度呈显著正相关(P<0.01),与土壤温度的相关性(P<0.05)次之。S1与S3处理的N_(2)O排放通量均与土壤温度呈显著相关(P均小于0.05),2024年二者相关性发生明显变化,其中S1处理中CH_(4)排放通量与土壤温度相关性减弱,但与pH呈显著负相关(P<0.01)。③不同处理间温室气体综合排放强度(greenhouse gas intensity,GHGI)存在显著差异(P均小于0.05),S2处理的GHGI在2023年与2024年试验中均最低,在维持产量的同时具有减排优势。研究显示,稻田CH_(4)与N_(2)O排放呈此消彼长关系,水分管理为核心调控因素,优化水分调控策略(如精准干湿交替)可实现协同减排。
