Obective:To evaluate the larvieidal.ovieidal and repellent properties of solvent extracts of marine sponge Cliona celala(C.celata)(Grant) against the malarial vector Anopheles stephensi(An.stephensi) Listen.Methods:Ma...Obective:To evaluate the larvieidal.ovieidal and repellent properties of solvent extracts of marine sponge Cliona celala(C.celata)(Grant) against the malarial vector Anopheles stephensi(An.stephensi) Listen.Methods:Marine sponge C.celala was thoroughly washed with distilled water and shade dried for 48 h.Then the sponges were homogenized and extracted sequentially with hexane,ethyl acetate and methanol,Lanvicidal and ovicidal activities were tested at four different concentrations viz.,62.5.125.0.250.0 and 500.0 ppm.for repellent study extracts were taken in three different concentrations viz..5.0.2.5.1.0 mg/cm at.Results:Among the three solvent extracts of C.celata,methanol extract showed thc highest lanvieidal activity at 500 ppm against the fourth inslar larvae of An.stephensi.The LC_(50)and LC_(90) values of C.celata methanol extract were recorded as 80.61 and 220.81 ppm against An.stephensi larvae respectively.High ovieidal activity of 91.2%was recorded al 500 ppm concentration of methanol extract.The haxane extract was found to be the most effective protectant against the adult female mosquitoes of An.stephensi.The mean protection time recorded in hexane extract was up to 245 min at 5 mg/cm^2 dosage against An.Stephensi adults.Conclusions:The screening results suggest that the hexane and methanol extracts of C.celata arc promising in mosquito control.Considering these bioaclivitics.C.celata could the probed further to obtain some novel pesticidal molecules.展开更多
Objective:To test three marine sponges Halichondria glabrata Keller,1891;Spirastrellapachyspira(S.pachyspira)Levi,1958 and Cliona lobata Hancock,1849 for the presence of the acetylcholinesterase(AChE)in both young and...Objective:To test three marine sponges Halichondria glabrata Keller,1891;Spirastrellapachyspira(S.pachyspira)Levi,1958 and Cliona lobata Hancock,1849 for the presence of the acetylcholinesterase(AChE)in both young and developed samples from western coastal area of India.S.pachyspira methanolic extract was selected for anti/pro angiogenic activity.Methods:They were evaluated for AChE activity using Ellman’s assay based on production of yellow colored 5-thio-2-nitrobenzoate.Purification of the enzyme was planned using ammonium sulphate precipitation and characterization by sodium dodecyl sulfate polyacrylamide gel electrophoresis.Chorioallantoic membrane(ChAM)assay model was used for angiogenic/antiangiogenic testing.Results:All the three sponges showed good specific enzyme activity and S.pachyspira contained maximum specific enzyme activity.Sixty percent of ammonium sulphate precipitation of crude protein sample gave single band at 66 kDa corresponding to the true AChE.ChAM assay was performed at 62.5,125.0 and 250.0μg/mL.Dosage beyond 250μg/mL extract showed toxic response with anti angiogenic activity at all the concentrations.Conclusions:AChE activity was detected in all samples.Extract showed good anti-angiogenic response at 62.5μg/mL.Extract was highly toxic affecting microvasculature of ChAM as well as normal growth and development of the embryo at 500μg/mL.With further characterization of bioactive compounds from the extract of S.pachyspira,the compounds can be developed for anti tumor activity.展开更多
基金Entomology Research Institute,Loyola College for financial assistance
文摘Obective:To evaluate the larvieidal.ovieidal and repellent properties of solvent extracts of marine sponge Cliona celala(C.celata)(Grant) against the malarial vector Anopheles stephensi(An.stephensi) Listen.Methods:Marine sponge C.celala was thoroughly washed with distilled water and shade dried for 48 h.Then the sponges were homogenized and extracted sequentially with hexane,ethyl acetate and methanol,Lanvicidal and ovicidal activities were tested at four different concentrations viz.,62.5.125.0.250.0 and 500.0 ppm.for repellent study extracts were taken in three different concentrations viz..5.0.2.5.1.0 mg/cm at.Results:Among the three solvent extracts of C.celata,methanol extract showed thc highest lanvieidal activity at 500 ppm against the fourth inslar larvae of An.stephensi.The LC_(50)and LC_(90) values of C.celata methanol extract were recorded as 80.61 and 220.81 ppm against An.stephensi larvae respectively.High ovieidal activity of 91.2%was recorded al 500 ppm concentration of methanol extract.The haxane extract was found to be the most effective protectant against the adult female mosquitoes of An.stephensi.The mean protection time recorded in hexane extract was up to 245 min at 5 mg/cm^2 dosage against An.Stephensi adults.Conclusions:The screening results suggest that the hexane and methanol extracts of C.celata arc promising in mosquito control.Considering these bioaclivitics.C.celata could the probed further to obtain some novel pesticidal molecules.
文摘Objective:To test three marine sponges Halichondria glabrata Keller,1891;Spirastrellapachyspira(S.pachyspira)Levi,1958 and Cliona lobata Hancock,1849 for the presence of the acetylcholinesterase(AChE)in both young and developed samples from western coastal area of India.S.pachyspira methanolic extract was selected for anti/pro angiogenic activity.Methods:They were evaluated for AChE activity using Ellman’s assay based on production of yellow colored 5-thio-2-nitrobenzoate.Purification of the enzyme was planned using ammonium sulphate precipitation and characterization by sodium dodecyl sulfate polyacrylamide gel electrophoresis.Chorioallantoic membrane(ChAM)assay model was used for angiogenic/antiangiogenic testing.Results:All the three sponges showed good specific enzyme activity and S.pachyspira contained maximum specific enzyme activity.Sixty percent of ammonium sulphate precipitation of crude protein sample gave single band at 66 kDa corresponding to the true AChE.ChAM assay was performed at 62.5,125.0 and 250.0μg/mL.Dosage beyond 250μg/mL extract showed toxic response with anti angiogenic activity at all the concentrations.Conclusions:AChE activity was detected in all samples.Extract showed good anti-angiogenic response at 62.5μg/mL.Extract was highly toxic affecting microvasculature of ChAM as well as normal growth and development of the embryo at 500μg/mL.With further characterization of bioactive compounds from the extract of S.pachyspira,the compounds can be developed for anti tumor activity.
