Background:Traditional Chinese medicine(TCM)syndrome,also named syndrome,are comprehensive and integral analyses of clinical information which helps to guide different individualized treatment prescriptions.Methods:Th...Background:Traditional Chinese medicine(TCM)syndrome,also named syndrome,are comprehensive and integral analyses of clinical information which helps to guide different individualized treatment prescriptions.Methods:Thirty healthy controls and 80 colorectal cancer(CRC)patients(including 33 Spleen Qi Deficiency syndrome,23 Dampness Heat syndrome,17 Blood Stasis syndrome and 7 other syndrome)were enrolled into this study.Human mRNAs were extracted from peripheral blood mononuclear cells.The gene expression for CRC patients with different TCM syndrome was determined by microarray and qRT-PCR.Results:Spleen Qi Deficiency,Dampness Heat and Blood Stasis were the most common syndromes in CRC patients.There is a significant difference was found in mRNA expression levels(especially for PIK3CA,STAT3,SOX9 and KDM5C)among Spleen Qi Deficiency,Dampness Heat and Blood Stasis syndrome groups.The higher mRNA levels of JNK1,TP53,MLH1,MSH6,PMS2,SOCS3,TCF7L2,FAM123B,PSAP,FBXW7,SALL4 and the lower expression of inflammatory cytokine IL-6 were found in Spleen Qi Deficiency group but not other syndrome types.The higher mRNA levels of KRAS,MUC16,EGFR,GRASP65,PIK3CA,MAPK7,CD24,STAT3,SLC11A1,Bcl-2,TXNDC17 and some inflammatory cytokines(IL-6,IL-23,TNF-a,CXCR4)were found in Dampness Heat group but not other syndrome types.Blood Stasis syndrome showed higher expression of SOX9,MLH1,MSH6,KDM5C,PCDH11X,PSAP and SALL4,and lower mRNA levels of PIK3CA,CD24,STAT3,CXCR4,TXNDC17 and TP53.The CRC patients with Dampness Heat syndrome might have a poor prognosis than other syndrome types.Conclusion:The identification of syndrome conditions had different impacts on CRC prognosis,and which might be related with different mRNA expression levels.Some oncogenes and pro-inflammatory cytokines were highly expressed in Dampness Heat group but not other syndrome types,suggesting that the CRC patients with Dampness Heat syndrome might have a poor prognosis.Our results prelimitarily uncovered the molecular basis of syndrome differences in CRC prognosis,a better understanding for TCM treatment of CRC.展开更多
Objective: to analyze the clinical distribution and drug resistance of Staphylococcus aureus (SAU) and Methicillin Resistant Staphylococcus aureus (MRSA). Methods: we retrospectively analyzed the clinically separated ...Objective: to analyze the clinical distribution and drug resistance of Staphylococcus aureus (SAU) and Methicillin Resistant Staphylococcus aureus (MRSA). Methods: we retrospectively analyzed the clinically separated SAU and clinical and drug susceptibility data in our hospital from 2016 to 2020, and used the WHONET5.6 software for statistical analysis. Results: a total of 1138 SAU strains were isolated in 5 years, of which 25.6% (291/1138) were MRSA. The positive rates of MRSA from 2016 to 2020 were 32.0%, 23.8%, 21.0%, 31.6%, and 21.4%. Wound pus (46.9%) was the most common source of SAU specimens, followed by respiratory tract (29.3%) and blood (11.1%), and the departments were mainly orthopedics (15.2%), ICU (9.2%) and general surgery (8.5%), among them, MRSA mainly isolated from respiratory tract (41.9%), wound pus (39.2%) and blood (9.6%) specimens. The departments with high MRSA positive rates are cardiothoracic surgery (59.1%), respiratory medicine (52.9%) and neurosurgery (49.6%). The resistance rates of SAU to gentamicin, rifampicin, levofloxacin, trimethoprim-sulfamethoxazole and tetracycline showed a certain downward trend, and no strains insensitive to vancomycin, teicoplanin and linezolid were found. The resistance rate of MRSA to most antibacterial drugs is higher than that of Methicillin Sensitive Staphylococcus aureus (MSSA), and the resistance rate to penicillin, erythromycin and clindamycin is high (> 50%). The resistance rate to gentamicin, levofloxacin and tetracycline is high (> 30%). Conclusion: the resistance of SAU to most antibacterial drugs and the positive rate of MRSA show a downward trend. glycopeptides and linezolid are still the best antibacterial drugs, which should be considered for severe infections.展开更多
基金This research was supported by grants from National Natural Science Foundation of China(grant No.81874380,81672932,81730108 and 81973635)Zhejiang Provincial Natural Science Foundation of China for Distinguished Young Scholars(grant No.LR18H160001)+6 种基金Zhejiang Province Science and Technology Project of TCM(grant No.2019ZZ016)Zhejiang Province Medical Science and Technology Project(grant No.2017RC007)Talent Project of Zhejiang Association for Science and Technology(grant No.2017YCGC002)Key Project of Hangzhou Ministry of Science and Technology(grant No.20162013A07)Zhejiang Provincial Project for the Key Discipline of Traditional Chinese Medicine(grant No.2017-XK-A09)the Open Project Program of Jiangsu Key Laboratory for Pharmacology and Safety Evaluation of Chinese Materia Medica(No.JKLPSE201807)the Project of the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD).
文摘Background:Traditional Chinese medicine(TCM)syndrome,also named syndrome,are comprehensive and integral analyses of clinical information which helps to guide different individualized treatment prescriptions.Methods:Thirty healthy controls and 80 colorectal cancer(CRC)patients(including 33 Spleen Qi Deficiency syndrome,23 Dampness Heat syndrome,17 Blood Stasis syndrome and 7 other syndrome)were enrolled into this study.Human mRNAs were extracted from peripheral blood mononuclear cells.The gene expression for CRC patients with different TCM syndrome was determined by microarray and qRT-PCR.Results:Spleen Qi Deficiency,Dampness Heat and Blood Stasis were the most common syndromes in CRC patients.There is a significant difference was found in mRNA expression levels(especially for PIK3CA,STAT3,SOX9 and KDM5C)among Spleen Qi Deficiency,Dampness Heat and Blood Stasis syndrome groups.The higher mRNA levels of JNK1,TP53,MLH1,MSH6,PMS2,SOCS3,TCF7L2,FAM123B,PSAP,FBXW7,SALL4 and the lower expression of inflammatory cytokine IL-6 were found in Spleen Qi Deficiency group but not other syndrome types.The higher mRNA levels of KRAS,MUC16,EGFR,GRASP65,PIK3CA,MAPK7,CD24,STAT3,SLC11A1,Bcl-2,TXNDC17 and some inflammatory cytokines(IL-6,IL-23,TNF-a,CXCR4)were found in Dampness Heat group but not other syndrome types.Blood Stasis syndrome showed higher expression of SOX9,MLH1,MSH6,KDM5C,PCDH11X,PSAP and SALL4,and lower mRNA levels of PIK3CA,CD24,STAT3,CXCR4,TXNDC17 and TP53.The CRC patients with Dampness Heat syndrome might have a poor prognosis than other syndrome types.Conclusion:The identification of syndrome conditions had different impacts on CRC prognosis,and which might be related with different mRNA expression levels.Some oncogenes and pro-inflammatory cytokines were highly expressed in Dampness Heat group but not other syndrome types,suggesting that the CRC patients with Dampness Heat syndrome might have a poor prognosis.Our results prelimitarily uncovered the molecular basis of syndrome differences in CRC prognosis,a better understanding for TCM treatment of CRC.
文摘Objective: to analyze the clinical distribution and drug resistance of Staphylococcus aureus (SAU) and Methicillin Resistant Staphylococcus aureus (MRSA). Methods: we retrospectively analyzed the clinically separated SAU and clinical and drug susceptibility data in our hospital from 2016 to 2020, and used the WHONET5.6 software for statistical analysis. Results: a total of 1138 SAU strains were isolated in 5 years, of which 25.6% (291/1138) were MRSA. The positive rates of MRSA from 2016 to 2020 were 32.0%, 23.8%, 21.0%, 31.6%, and 21.4%. Wound pus (46.9%) was the most common source of SAU specimens, followed by respiratory tract (29.3%) and blood (11.1%), and the departments were mainly orthopedics (15.2%), ICU (9.2%) and general surgery (8.5%), among them, MRSA mainly isolated from respiratory tract (41.9%), wound pus (39.2%) and blood (9.6%) specimens. The departments with high MRSA positive rates are cardiothoracic surgery (59.1%), respiratory medicine (52.9%) and neurosurgery (49.6%). The resistance rates of SAU to gentamicin, rifampicin, levofloxacin, trimethoprim-sulfamethoxazole and tetracycline showed a certain downward trend, and no strains insensitive to vancomycin, teicoplanin and linezolid were found. The resistance rate of MRSA to most antibacterial drugs is higher than that of Methicillin Sensitive Staphylococcus aureus (MSSA), and the resistance rate to penicillin, erythromycin and clindamycin is high (> 50%). The resistance rate to gentamicin, levofloxacin and tetracycline is high (> 30%). Conclusion: the resistance of SAU to most antibacterial drugs and the positive rate of MRSA show a downward trend. glycopeptides and linezolid are still the best antibacterial drugs, which should be considered for severe infections.
