Flower blight on anthurium(Anthurium andraeanum)was observed during August 2018 on an anthurium cultivation farm in the Songkhla Province of southern Thailand.The fungal isolate was identified as Neopestalotiopsis cla...Flower blight on anthurium(Anthurium andraeanum)was observed during August 2018 on an anthurium cultivation farm in the Songkhla Province of southern Thailand.The fungal isolate was identified as Neopestalotiopsis clavispora based on the morphology and DNA sequence of the internal transcribed spacer(ITS),translation elongation factor 1-α(tef1-α),andβ-tubulin(tub)genes.The phylogenetic tree,based on the combined sequences of ITS,tef1-α,and tub,confirmed this pathogen as N.clavispora.Pathogenicity of the species was confirmed according to Koch’s postulate:N.clavispora could infect anthurium.To the best of our knowledge,this is the first report of N.clavispora as a pathogen of anthurium.展开更多
[Objectives]The study was to confirm the etiological agent of leaf spot on banana in Yunnan Province of China.[Methods]Fungus isolates were isolated from the diseased tissues,and cultured to observe the morphological ...[Objectives]The study was to confirm the etiological agent of leaf spot on banana in Yunnan Province of China.[Methods]Fungus isolates were isolated from the diseased tissues,and cultured to observe the morphological characteristics of colony and spore.Furthermore,phylogenetic analyses and pathogenicity test were also conducted to confirm the pathogen.[Results]The fungus isolated from the diseased tissues was identified as Neopestalotiopsis clavispora.[Conclusions]N.clavispora is a new pathogen causing leaf spot of banana.This research provides the first description of N.clavispora as a causal agent on banana in China,and adds new insights related to the host range of N.clavispora.展开更多
Microbial production of chiralγ-decalactone has received considerable attention due to its high market value.However,the inhibition of substrates and products on microorganisms has significantly limited the productio...Microbial production of chiralγ-decalactone has received considerable attention due to its high market value.However,the inhibition of substrates and products on microorganisms has significantly limited the production of chiralγ-decalactone.In this study,a green biphasic fermentation system containing 1-butyl-3-methylimidazo-lium tetrafluoroborate([C_(4)MIM]PF6)was developed to increase the synthesis of(R)-γ-decalactone from rici-noleic acid catalyzed by Clavispora lusitaniae YJ26.Under optimal conditions,the titer ofγ-decalactone obtained using the biphasic system reached 2.04 g/L,which is 2.4 times that of the traditional aqueous fermentation system,and showed high enantioselectivity(e.e.>99.0%).Fluorescence microscopy and flow cytometry results demonstrated that the addition of[C_(4)MIM]PF6 significantly alleviated the damage of substrate and product to cell membrane and improved cell viability,thus increasing the titer ofγ-decalactone.Furthermore,comparative transcriptomic analysis results suggested that the improved mechanism may be attributed to the up-regulation of genes associated with cell membrane component synthesis or cell tolerance enhancement.Overall,the experi-mental results provide valuable insights into the efficient microbial production of chiral decalactone in an ionic liquids-containing biphasic fermentation system.展开更多
基金supported by Prince of Songkla Universitythe Center of Excellence in Agricultural and Natural Resources Biotechnology(Grant No.CoE-ANRB)phase 3。
文摘Flower blight on anthurium(Anthurium andraeanum)was observed during August 2018 on an anthurium cultivation farm in the Songkhla Province of southern Thailand.The fungal isolate was identified as Neopestalotiopsis clavispora based on the morphology and DNA sequence of the internal transcribed spacer(ITS),translation elongation factor 1-α(tef1-α),andβ-tubulin(tub)genes.The phylogenetic tree,based on the combined sequences of ITS,tef1-α,and tub,confirmed this pathogen as N.clavispora.Pathogenicity of the species was confirmed according to Koch’s postulate:N.clavispora could infect anthurium.To the best of our knowledge,this is the first report of N.clavispora as a pathogen of anthurium.
基金Supported by High level Talents Project of Hainan Provincial Natural Science Foundation of China(2019RC278)The Earmarked Fund for CARS(CARS-31).
文摘[Objectives]The study was to confirm the etiological agent of leaf spot on banana in Yunnan Province of China.[Methods]Fungus isolates were isolated from the diseased tissues,and cultured to observe the morphological characteristics of colony and spore.Furthermore,phylogenetic analyses and pathogenicity test were also conducted to confirm the pathogen.[Results]The fungus isolated from the diseased tissues was identified as Neopestalotiopsis clavispora.[Conclusions]N.clavispora is a new pathogen causing leaf spot of banana.This research provides the first description of N.clavispora as a causal agent on banana in China,and adds new insights related to the host range of N.clavispora.
基金supported by the Key Realm R&D Program of Guang-Dong Province(2022B0202050003)Guangdong Basic and Applied Basic Research Foundation(2022A1515110710)+1 种基金Science and Technology Projects in Guangzhou(2023A04J0745)Guangdong Provincial Key Laboratory of Lingnan Specialty Food Science and Technology(2021B1212040013).
文摘Microbial production of chiralγ-decalactone has received considerable attention due to its high market value.However,the inhibition of substrates and products on microorganisms has significantly limited the production of chiralγ-decalactone.In this study,a green biphasic fermentation system containing 1-butyl-3-methylimidazo-lium tetrafluoroborate([C_(4)MIM]PF6)was developed to increase the synthesis of(R)-γ-decalactone from rici-noleic acid catalyzed by Clavispora lusitaniae YJ26.Under optimal conditions,the titer ofγ-decalactone obtained using the biphasic system reached 2.04 g/L,which is 2.4 times that of the traditional aqueous fermentation system,and showed high enantioselectivity(e.e.>99.0%).Fluorescence microscopy and flow cytometry results demonstrated that the addition of[C_(4)MIM]PF6 significantly alleviated the damage of substrate and product to cell membrane and improved cell viability,thus increasing the titer ofγ-decalactone.Furthermore,comparative transcriptomic analysis results suggested that the improved mechanism may be attributed to the up-regulation of genes associated with cell membrane component synthesis or cell tolerance enhancement.Overall,the experi-mental results provide valuable insights into the efficient microbial production of chiral decalactone in an ionic liquids-containing biphasic fermentation system.
