Classical Chinese gardens are purposely designed so that no matter where you stand,you can never see the whole thing at once.The mystery and the discovery have helped the classical gardens of China,especially those in...Classical Chinese gardens are purposely designed so that no matter where you stand,you can never see the whole thing at once.The mystery and the discovery have helped the classical gardens of China,especially those in Suzhou,remain popular attractions after all these years.Put simply,the aim of a classical Chinese garden is to deepen one?s admiration of nature.Besides just relaxing in nature,the classical Chinese gardens have another purpose-to inspire artistic expression like poetry,literature or painting.Nearly all Chinese gardens feature the things in the list below.展开更多
A Receiver Operating Characteristic(ROC)analysis of a power is important and useful in clinical trials.A Classical Conditional Power(CCP)is a probability of a classical rejection region given values of true treatment ...A Receiver Operating Characteristic(ROC)analysis of a power is important and useful in clinical trials.A Classical Conditional Power(CCP)is a probability of a classical rejection region given values of true treatment effect and interim result.For hypotheses and reversed hypotheses under normal models,we obtain analytical expressions of the ROC curves of the CCP,find optimal ROC curves of the CCP,investigate the superiority of the ROC curves of the CCP,calculate critical values of the False Positive Rate(FPR),True Positive Rate(TPR),and cutoff of the optimal CCP,and give go/no go decisions at the interim of the optimal CCP.In addition,extensive numerical experiments are carried out to exemplify our theoretical results.Finally,a real data example is performed to illustrate the go/no go decisions of the optimal CCP.展开更多
Explorations into new electrolytes have highlighted the critical impact of solvation structure on battery performance,Classical molecular dynamics(CMD)using semi-empirical force fields has become an essential tool for...Explorations into new electrolytes have highlighted the critical impact of solvation structure on battery performance,Classical molecular dynamics(CMD)using semi-empirical force fields has become an essential tool for simulating solvation structures.However,mainstream force fields often lack accuracy in describing strong ion-solvent interactions,causing disparities between CMD simulations and experimental observations.Although some empirical methods have been employed in some of the studies to address this issue,their effectiveness has been limited.Our CMD research,supported by quantum chemical calculations and experimental data,reveals that the solvation structure is influenced not only by the charge model but also by the polarization description.Previous empirical approaches that focused solely on adjusting ion-solvent interaction strengths overlooked the importance of polarization effects.Building on this insight,we propose integrating the Drude polarization model into mainstream force fields and verify its feasibility in carbonate,ether,and nitrile electrolytes.Our experimental results demonstrate that this approach significantly enhances the accuracy of CMD-simulated solvation structures.This work is expected to provide a more reliable CMD method for electrolyte design,shielding researchers from the pitfalls of erroneous simulation outcomes.展开更多
This paper concerns the Cauchy problem of 3D compressible micropolar fluids in the whole space R^(3). For regular initial data with m0E0 is suitable small, where m0 and E0 represent the upper bound of initial density ...This paper concerns the Cauchy problem of 3D compressible micropolar fluids in the whole space R^(3). For regular initial data with m0E0 is suitable small, where m0 and E0 represent the upper bound of initial density and initial energy, we prove that if ρ0 ∈ Lγ ∩ H3 with γ ∈ (1, 6), then the problem possesses a unique global classical solution on R^(3) × [0, T] with any T ∈ (0, ∞). It’s worth noting that both the vacuum states and possible random largeness of initial energy are allowed.展开更多
This article examines the development of geomorpho-logical and hydrological research on karst landscapes in Central and Southeast Europe in the period before 1914.It provides novel insights into the introduction of th...This article examines the development of geomorpho-logical and hydrological research on karst landscapes in Central and Southeast Europe in the period before 1914.It provides novel insights into the introduction of the term“karst,”originally the name of a cavernous limestone plateau east of the Adriatic city of Trieste,as a universal model for comparing and understanding dissolutional features in soluble rocks.Based on a critical reappraisal of the sem-inal work“The Karst Phenomenon”(1893)by the Serbian geoscientist Jovan Cvijić(1865-1928),submitted as a Ph.D.thesis at the University of Vienna,my essay argues for a reassessment of the beginnings of karst sciences.It gives more attention to research as a cooperative enterprise and to the interplay between the internationalization of geosci-entific knowledge and the emerging national claims to the Balkan Peninsula and its inhabitants.Approaching the topic from a history of science perspective,I will analyze the epistemic,political,and social dimensions of early karst research at three different stages,namely,its emergence as an imperial undertaking in the Habsburg Monarchy,its synthesis and systematization through Cvijić’s“Karst Phenomenon,”and the final establishment of the Dinarides’northwestern part as the“Classical”Karst.展开更多
Grass constitutes a vital poetic imagery in classical Chinese poetry,embodying multifaceted symbolic connotations ranging from the tenacity of life to sentiments of separation and nostalgic longing.The translation of ...Grass constitutes a vital poetic imagery in classical Chinese poetry,embodying multifaceted symbolic connotations ranging from the tenacity of life to sentiments of separation and nostalgic longing.The translation of this botanical motif necessitates not merely lexical equivalence,but more importantly,the transmission of its profound cultural resonance and aesthetic essence.This study posits that effective rendition of grass imagery should adopt an integrative approach synthesizing the objectives of cultural translation with the intrinsic aesthetic characteristics of classical poetry.Through systematic analysis of the cultural semiotics embedded in grass symbolism,the research investigates practical translation techniques at lexical,syntactic,and stylistic dimensions.The findings aim to contribute to the theoretical framework of cultural image translation in Chinese poetic tradition while providing methodological references for cross-cultural interpretation of classical verse.By bridging cultural semiotics with translation praxis,this investigation seeks to advance the intercultural communication of Chinese poetic heritage through nuanced treatment of its botanical symbolism.展开更多
More than a simple concert pianist,Wilson Chu is an Indonesian musical diplomacy force.As a distinguished performer,composer,and educator,Wilson has left an indelible mark on the international stage by seamlessly blen...More than a simple concert pianist,Wilson Chu is an Indonesian musical diplomacy force.As a distinguished performer,composer,and educator,Wilson has left an indelible mark on the international stage by seamlessly blending Western classical music with Southeast Asian tradition.Since 2019,he has been the youngest Associate Professor of Piano at the College of Chinese and ASEAN Arts(CCAA)at Chengdu University,where he has dedicated himself to shaping the next generation of musicians.展开更多
The presence of background classical sources affects quantum field theory significantly in different ways.Neutrino oscillation is a phenomenon that confirms that neutrinos are massive fermions in nature,a celebrated r...The presence of background classical sources affects quantum field theory significantly in different ways.Neutrino oscillation is a phenomenon that confirms that neutrinos are massive fermions in nature,a celebrated result in modern physics.Neutrino oscillation plays an important role in many astrophysical observations.However,the interactions between the background classical sources with neutrinos are not often considered.In the present article,we show the effect of some classical sources,namely matter currents,electromagnetic waves,torsion,and gravitational waves on neutrino oscillation.It is shown explicitly that the above sources can change the helicity state of neutrinos during neutrino oscillation.展开更多
We investigate the mixed-state entanglement between two spins embedded in the XXZ Heisenberg chain under thermal equilibrium.By deriving an analytical expression for the entanglement of two-spin thermal states and ext...We investigate the mixed-state entanglement between two spins embedded in the XXZ Heisenberg chain under thermal equilibrium.By deriving an analytical expression for the entanglement of two-spin thermal states and extending this analysis to larger spin chains,we demonstrate that mixed-state entanglement is profoundly shaped by both disorder and temperature.Our results reveal a sharp distinction between many-body localized and ergodic phases,with entanglement vanishing above diferent fnite temperature thresholds.Furthermore,by analyzing non-adjacent spins,we uncover an approximate exponential decay of entanglement with separation.This work advances the understanding of the quantum-to-classical transition by linking the entanglement properties of small subsystems to the broader thermal environment,ofering an explanation for the absence of entanglement in macroscopic systems.These fndings provide critical insights into quantum many-body physics,bridging concepts from thermalization,localization,and quantum information theory.展开更多
Molecular cloning remains a cornerstone technique in genetic engineering and synthetic biology.In this study,we conducted a systematic comparative analysis between the classical cloning method and the Golden Gate asse...Molecular cloning remains a cornerstone technique in genetic engineering and synthetic biology.In this study,we conducted a systematic comparative analysis between the classical cloning method and the Golden Gate assembly technique,utilizing Escherichia coli as the model organism.Through polymerase chain reaction(PCR)amplification,restriction enzyme digestion,ligation,transformation,and Sanger sequencing,we assessed the operational efficiency and cloning fidelity of both strategies.Our results demonstrated that Golden Gate assembly,leveraging type IIS restriction enzymes and simultaneous ligation,significantly enhanced cloning efficiency and precision,particularly for seamless multi-fragment assembly.In contrast,the classical cloning approach maintained certain advantages in simplicity and robustness for specific experimental conditions.Challenges encountered during transformation and sequencing highlighted the critical impact of technical accuracy on experimental outcomes.This study underscores the importance of selecting appropriate cloning methodologies tailored to experimental objectives and laboratory capabilities,providing a foundation for optimized molecular cloning workflows in future synthetic biology and biotechnology applications.展开更多
Human rights are both a hallmark of progress in human civilization and a shared achievement of all civilizations.Throughout history,civilizations,including the Chinese civilization,have continuously explored fundament...Human rights are both a hallmark of progress in human civilization and a shared achievement of all civilizations.Throughout history,civilizations,including the Chinese civilization,have continuously explored fundamental questions about human life,value,and dignity,collectively shaping the foundation of global human rights civilization.On November 20,2024,the International Academic Conference on the Ideas of Human Rights in Ancient Chinese Classics was held at the Yuelu Academy of Hunan University.Attendants explored the historical origin and creative transformation of China’s human rights civilization from four dimensions,namely comparative studies of human rights civilizations in China and abroad,human rights concepts in Confucian classics and historical records,human rights concepts in Chinese philosophical and miscellaneous works,and human rights concepts in legal texts.The conference examined the historical connection between human rights concepts in ancient Chinese classics and the shared values of humanity,aiming to promote global human rights dialogue and mutual learning through civilizational exchanges.By bridging“ancient and modern,East and West,”it illuminated the civilizational foundations and Chinese wisdom underlying global human rights governance.展开更多
Understanding the thermodynamic behavior of complex fluids in confined environments is critical for various industrial and natural processes including but not limited to polymer flooding enhanced oil recovery(EOR).In ...Understanding the thermodynamic behavior of complex fluids in confined environments is critical for various industrial and natural processes including but not limited to polymer flooding enhanced oil recovery(EOR).In this work,we develop Atif-V2.0,an extended classical density functional theory(cDFT)framework that integrates the interfacial statistical associating fluid theory(iSAFT)to model multicomponent associating fluids composed of water-soluble polymers,alkanes,and water.Building on the original theoretical framework of Atif for modeling nanoconfined inhomogeneous fluids,Atif-V2.0 embeds explicit solvent and captures additional physical interactions-hydrogen bonding,which are critical in associating fluid systems.The other key feature of Atif-V2.0 is its ability to account for polymer topology.We demonstrate its capability by predicting the equilibrium structure and thermodynamic behavior of branched hydrolyzed polyacrylamide solutions near hard walls with various branching topologies,which provides a robust theoretical tool for the rational design of EOR polymers.展开更多
Objective Randomized controlled trials(RCTs)of Chinese patent medicines and classic tra-ditional Chinese medicine prescriptions were systematically reviewed from both Chinese and English journals published in 2023.A p...Objective Randomized controlled trials(RCTs)of Chinese patent medicines and classic tra-ditional Chinese medicine prescriptions were systematically reviewed from both Chinese and English journals published in 2023.A preliminary summary and evaluation were conducted on the generation and translation of clinical evidence for these treatments.This analysis aims to inform future research on clinical efficacy evaluation and guide the rational application of evidence.Methods RCTs of Chinese patent medicines and classic traditional Chinese prescriptions published in 2023 were comprehensively retrieved from the Artificial Intelligence Clinical Evi-dence Database for Chinese Patent Medicine(AICED-CPM),with supplementary searches conducted in China National Knowledge Infrastructure(CNKI),Wanfang Data,Chinese Sci-ence and Technology Journal Database(VIP),Chinese Biomedical Literature Database(SinoMed),Cochrane Library,PubMed,Embase,and Web of Science.The study characteris-tics and methodological quality of these RCTs were systematically analyzed and evaluated.Results A total of 1443 RCTs of Chinese patent medicines were included,comprising 1399 Chinese articles and 44 English articles.Additionally,334 RCTs of classic traditional Chinese medicine prescriptions were found,with 331 published in Chinese and 3 in English.196567 participants were included,covering 585 types of Chinese patent medicines(487 oral,61 in-jectable,and 37 topical)and 179 classic traditional Chinese medicine prescriptions.The in-volved studies encompassed 22 types of diseases,with research primarily focusing on dis-eases of the circulatory system,the respiratory system,and the genitourinary system.The sample sizes ranged from 18 to 3777 participants,and most studies were conducted at a sin-gle center.Methodologically,the implementation of allocation concealment and blinding re-mained insufficiently emphasized.Conclusion Overall,compared with 2022,both the number of RCT publications and their methodological quality have improved in 2023,with heightened attention to research on diseases of the genitourinary system.However,quality control and standardized management in the design and implementation processes still require enhancement to produce more high-quality clinical evidence and accelerate the translation and application of this evidence.展开更多
BACKGROUND Surgically created arterio-venous fistulas(AVFs)are the gold standard for haemodialysis access for patients with end-stage renal disease.Standard practice of AVF creation involves selecting the non-dominant...BACKGROUND Surgically created arterio-venous fistulas(AVFs)are the gold standard for haemodialysis access for patients with end-stage renal disease.Standard practice of AVF creation involves selecting the non-dominant upper limb and starting with most distally with radio-cephalic arterio-venous fistula.The primary patency rate of radio-cephalic arterio-venous fistula varies from 20%-25%.It has been suggested the neointimal hyperplasia at the mobilized venous segment causes stenosis of the anastomosis.Therefore,the radial artery deviation and reimplantation(RADAR)technique,in which the vein is minimally mobilized,should result in a higher success rate.AIM To compare the RADAR technique with classical technique in creation of AVF including:(1)Success rate;(2)Time to maturation;(3)Duration of surgery;and(4)Complication rate.METHODS In our study we recruited 94 patients in two randomized groups and performed the AVF by the classical method or the RADAR method.RESULTS The RADAR group had higher primary success rate(P=0.007),less rate of complications(P=0.04),shorter duration of surgery(P=0.00)and early time to maturation(0.001)when compared with the classical group.The RADAR procedure is a safe and a more efficient alternative to the current classical method of AVF creation.Longer duration of follow-up is required to assess the long-term outcomes in the future.CONCLUSION The RADAR procedure is a safe and more efficient alternative to the current classical method of AVF creation.Longer duration of follow-up is required to assess the long-term outcomes in the future.展开更多
The basic human rights concepts in Chinese classics mainly encompass three categories:the concept of order and freedom influenced by the worldview of the harmony between humanity and nature,the concept of human subjec...The basic human rights concepts in Chinese classics mainly encompass three categories:the concept of order and freedom influenced by the worldview of the harmony between humanity and nature,the concept of human subjectivity and the source of human rights influenced by the people-oriented or benevolence-oriented values,as well as the concept of the relationship between human rights and state power guided by the outlook on life of the great unity of all under heaven(tianxia).Among them,the concept of order and freedom of the harmony between humanity and nature and modeling itself on nature is more beneficial to the stability and development of human society than the Western concept of order and freedom under“rational choice.”The norm prototype of“Do not do to others what you do not want others to do to you”formed the Article 4 of the French Declaration of the Rights of Man and of the Citizen in 1789,and the concepts of“benevolence”and“human superiority”were written into Article 1 of the Universal Declaration of Human Rights and the preamble of the Declaration of the United Nations Conference on the Human Environment,respectively,which have become the common spiritual wealth of all humankind.The ancient Chinese imperial examination system for selecting virtuous and capable people was basically consistent with the provisions on the political rights of citizens to hold public office stipulated in contemporary human rights documents,and was advanced in the world at that time.The contemporary Chinese system of“the state respects and protects human rights”is a system rooted in the Chinese historical and cultural traditions and is with the superiority of socialism.展开更多
The paper analyzes the English retranslation of The Classic on Tea from the perspective of cultural awareness and finds some misinterpretations and omissions of Chinese culture appeared in the translations of foreign ...The paper analyzes the English retranslation of The Classic on Tea from the perspective of cultural awareness and finds some misinterpretations and omissions of Chinese culture appeared in the translations of foreign scholars.Domestic translators have a deeper understanding of Chinese culture and their retranslations embody the increasing cultural awareness of the source language culture,mainly in seeking conceptual equivalence rather than lexical equivalence,choosing the most suitable translation according to the textual and cultural context,and focusing on cultural facsimile and readers’awareness.This study will help better spread the Chinese tea culture and help Chinese culture go global.展开更多
With the pilings of rockeries,bridge trails,doors and windows,pavilions,towers,walls,halls and studios,corridors and courtyards as the research subject,illustrated with a large number of pictures,the relationship betw...With the pilings of rockeries,bridge trails,doors and windows,pavilions,towers,walls,halls and studios,corridors and courtyards as the research subject,illustrated with a large number of pictures,the relationship between architecture and rockery in classical gardens was elaborated.In classical gardens,their harmonious,complementary and positive aspect was emphasized,and their opposite,repulsive and passive aspect was restricted,and even the passiveness could be transferred into positivity,so as to make the beauty of architecture and the beauty of nature coexist,coming to the state that the artificiality and the nature blend into one whole.The research was to provide reference for the design and construction of modern gardens.展开更多
文摘Classical Chinese gardens are purposely designed so that no matter where you stand,you can never see the whole thing at once.The mystery and the discovery have helped the classical gardens of China,especially those in Suzhou,remain popular attractions after all these years.Put simply,the aim of a classical Chinese garden is to deepen one?s admiration of nature.Besides just relaxing in nature,the classical Chinese gardens have another purpose-to inspire artistic expression like poetry,literature or painting.Nearly all Chinese gardens feature the things in the list below.
基金supported by the National Social Science Fund of China(Grand No.21XTJ001).
文摘A Receiver Operating Characteristic(ROC)analysis of a power is important and useful in clinical trials.A Classical Conditional Power(CCP)is a probability of a classical rejection region given values of true treatment effect and interim result.For hypotheses and reversed hypotheses under normal models,we obtain analytical expressions of the ROC curves of the CCP,find optimal ROC curves of the CCP,investigate the superiority of the ROC curves of the CCP,calculate critical values of the False Positive Rate(FPR),True Positive Rate(TPR),and cutoff of the optimal CCP,and give go/no go decisions at the interim of the optimal CCP.In addition,extensive numerical experiments are carried out to exemplify our theoretical results.Finally,a real data example is performed to illustrate the go/no go decisions of the optimal CCP.
基金supported by the Science and Technology Project of State Grid Corporation of China(5419-202199552A-0-5-ZN).
文摘Explorations into new electrolytes have highlighted the critical impact of solvation structure on battery performance,Classical molecular dynamics(CMD)using semi-empirical force fields has become an essential tool for simulating solvation structures.However,mainstream force fields often lack accuracy in describing strong ion-solvent interactions,causing disparities between CMD simulations and experimental observations.Although some empirical methods have been employed in some of the studies to address this issue,their effectiveness has been limited.Our CMD research,supported by quantum chemical calculations and experimental data,reveals that the solvation structure is influenced not only by the charge model but also by the polarization description.Previous empirical approaches that focused solely on adjusting ion-solvent interaction strengths overlooked the importance of polarization effects.Building on this insight,we propose integrating the Drude polarization model into mainstream force fields and verify its feasibility in carbonate,ether,and nitrile electrolytes.Our experimental results demonstrate that this approach significantly enhances the accuracy of CMD-simulated solvation structures.This work is expected to provide a more reliable CMD method for electrolyte design,shielding researchers from the pitfalls of erroneous simulation outcomes.
基金supported by the Natural Science Foundation of Shandong Province of China(ZR2024MA033ZR2021QA049).
文摘This paper concerns the Cauchy problem of 3D compressible micropolar fluids in the whole space R^(3). For regular initial data with m0E0 is suitable small, where m0 and E0 represent the upper bound of initial density and initial energy, we prove that if ρ0 ∈ Lγ ∩ H3 with γ ∈ (1, 6), then the problem possesses a unique global classical solution on R^(3) × [0, T] with any T ∈ (0, ∞). It’s worth noting that both the vacuum states and possible random largeness of initial energy are allowed.
文摘This article examines the development of geomorpho-logical and hydrological research on karst landscapes in Central and Southeast Europe in the period before 1914.It provides novel insights into the introduction of the term“karst,”originally the name of a cavernous limestone plateau east of the Adriatic city of Trieste,as a universal model for comparing and understanding dissolutional features in soluble rocks.Based on a critical reappraisal of the sem-inal work“The Karst Phenomenon”(1893)by the Serbian geoscientist Jovan Cvijić(1865-1928),submitted as a Ph.D.thesis at the University of Vienna,my essay argues for a reassessment of the beginnings of karst sciences.It gives more attention to research as a cooperative enterprise and to the interplay between the internationalization of geosci-entific knowledge and the emerging national claims to the Balkan Peninsula and its inhabitants.Approaching the topic from a history of science perspective,I will analyze the epistemic,political,and social dimensions of early karst research at three different stages,namely,its emergence as an imperial undertaking in the Habsburg Monarchy,its synthesis and systematization through Cvijić’s“Karst Phenomenon,”and the final establishment of the Dinarides’northwestern part as the“Classical”Karst.
文摘Grass constitutes a vital poetic imagery in classical Chinese poetry,embodying multifaceted symbolic connotations ranging from the tenacity of life to sentiments of separation and nostalgic longing.The translation of this botanical motif necessitates not merely lexical equivalence,but more importantly,the transmission of its profound cultural resonance and aesthetic essence.This study posits that effective rendition of grass imagery should adopt an integrative approach synthesizing the objectives of cultural translation with the intrinsic aesthetic characteristics of classical poetry.Through systematic analysis of the cultural semiotics embedded in grass symbolism,the research investigates practical translation techniques at lexical,syntactic,and stylistic dimensions.The findings aim to contribute to the theoretical framework of cultural image translation in Chinese poetic tradition while providing methodological references for cross-cultural interpretation of classical verse.By bridging cultural semiotics with translation praxis,this investigation seeks to advance the intercultural communication of Chinese poetic heritage through nuanced treatment of its botanical symbolism.
文摘More than a simple concert pianist,Wilson Chu is an Indonesian musical diplomacy force.As a distinguished performer,composer,and educator,Wilson has left an indelible mark on the international stage by seamlessly blending Western classical music with Southeast Asian tradition.Since 2019,he has been the youngest Associate Professor of Piano at the College of Chinese and ASEAN Arts(CCAA)at Chengdu University,where he has dedicated himself to shaping the next generation of musicians.
基金supported by the SERB-Core Research Grant(Project RD/0122-SERB000-044)。
文摘The presence of background classical sources affects quantum field theory significantly in different ways.Neutrino oscillation is a phenomenon that confirms that neutrinos are massive fermions in nature,a celebrated result in modern physics.Neutrino oscillation plays an important role in many astrophysical observations.However,the interactions between the background classical sources with neutrinos are not often considered.In the present article,we show the effect of some classical sources,namely matter currents,electromagnetic waves,torsion,and gravitational waves on neutrino oscillation.It is shown explicitly that the above sources can change the helicity state of neutrinos during neutrino oscillation.
基金supported by the National Natural Science Foundation of China(Grant Nos.92365202,12475011,and 11921005)the National Key R&D Program of China(Grant No.2024YFA1409002)Shanghai Municipal Science and Technology Major Project(Grant No.2019SHZDZX01)。
文摘We investigate the mixed-state entanglement between two spins embedded in the XXZ Heisenberg chain under thermal equilibrium.By deriving an analytical expression for the entanglement of two-spin thermal states and extending this analysis to larger spin chains,we demonstrate that mixed-state entanglement is profoundly shaped by both disorder and temperature.Our results reveal a sharp distinction between many-body localized and ergodic phases,with entanglement vanishing above diferent fnite temperature thresholds.Furthermore,by analyzing non-adjacent spins,we uncover an approximate exponential decay of entanglement with separation.This work advances the understanding of the quantum-to-classical transition by linking the entanglement properties of small subsystems to the broader thermal environment,ofering an explanation for the absence of entanglement in macroscopic systems.These fndings provide critical insights into quantum many-body physics,bridging concepts from thermalization,localization,and quantum information theory.
文摘Molecular cloning remains a cornerstone technique in genetic engineering and synthetic biology.In this study,we conducted a systematic comparative analysis between the classical cloning method and the Golden Gate assembly technique,utilizing Escherichia coli as the model organism.Through polymerase chain reaction(PCR)amplification,restriction enzyme digestion,ligation,transformation,and Sanger sequencing,we assessed the operational efficiency and cloning fidelity of both strategies.Our results demonstrated that Golden Gate assembly,leveraging type IIS restriction enzymes and simultaneous ligation,significantly enhanced cloning efficiency and precision,particularly for seamless multi-fragment assembly.In contrast,the classical cloning approach maintained certain advantages in simplicity and robustness for specific experimental conditions.Challenges encountered during transformation and sequencing highlighted the critical impact of technical accuracy on experimental outcomes.This study underscores the importance of selecting appropriate cloning methodologies tailored to experimental objectives and laboratory capabilities,providing a foundation for optimized molecular cloning workflows in future synthetic biology and biotechnology applications.
文摘Human rights are both a hallmark of progress in human civilization and a shared achievement of all civilizations.Throughout history,civilizations,including the Chinese civilization,have continuously explored fundamental questions about human life,value,and dignity,collectively shaping the foundation of global human rights civilization.On November 20,2024,the International Academic Conference on the Ideas of Human Rights in Ancient Chinese Classics was held at the Yuelu Academy of Hunan University.Attendants explored the historical origin and creative transformation of China’s human rights civilization from four dimensions,namely comparative studies of human rights civilizations in China and abroad,human rights concepts in Confucian classics and historical records,human rights concepts in Chinese philosophical and miscellaneous works,and human rights concepts in legal texts.The conference examined the historical connection between human rights concepts in ancient Chinese classics and the shared values of humanity,aiming to promote global human rights dialogue and mutual learning through civilizational exchanges.By bridging“ancient and modern,East and West,”it illuminated the civilizational foundations and Chinese wisdom underlying global human rights governance.
基金financially supported by the Key Technologies R&D Program of China National Offshore Oil Corporation(No.KJGG2021-0504)。
文摘Understanding the thermodynamic behavior of complex fluids in confined environments is critical for various industrial and natural processes including but not limited to polymer flooding enhanced oil recovery(EOR).In this work,we develop Atif-V2.0,an extended classical density functional theory(cDFT)framework that integrates the interfacial statistical associating fluid theory(iSAFT)to model multicomponent associating fluids composed of water-soluble polymers,alkanes,and water.Building on the original theoretical framework of Atif for modeling nanoconfined inhomogeneous fluids,Atif-V2.0 embeds explicit solvent and captures additional physical interactions-hydrogen bonding,which are critical in associating fluid systems.The other key feature of Atif-V2.0 is its ability to account for polymer topology.We demonstrate its capability by predicting the equilibrium structure and thermodynamic behavior of branched hydrolyzed polyacrylamide solutions near hard walls with various branching topologies,which provides a robust theoretical tool for the rational design of EOR polymers.
基金National Multidisciplinary Innovation Team of Traditional Chinese Medicine (ZYYCXTD-D-202204)Science and Technology Project of Haihe Laboratory of Modern Chinese Medicine (22HHZYSS00013)。
文摘Objective Randomized controlled trials(RCTs)of Chinese patent medicines and classic tra-ditional Chinese medicine prescriptions were systematically reviewed from both Chinese and English journals published in 2023.A preliminary summary and evaluation were conducted on the generation and translation of clinical evidence for these treatments.This analysis aims to inform future research on clinical efficacy evaluation and guide the rational application of evidence.Methods RCTs of Chinese patent medicines and classic traditional Chinese prescriptions published in 2023 were comprehensively retrieved from the Artificial Intelligence Clinical Evi-dence Database for Chinese Patent Medicine(AICED-CPM),with supplementary searches conducted in China National Knowledge Infrastructure(CNKI),Wanfang Data,Chinese Sci-ence and Technology Journal Database(VIP),Chinese Biomedical Literature Database(SinoMed),Cochrane Library,PubMed,Embase,and Web of Science.The study characteris-tics and methodological quality of these RCTs were systematically analyzed and evaluated.Results A total of 1443 RCTs of Chinese patent medicines were included,comprising 1399 Chinese articles and 44 English articles.Additionally,334 RCTs of classic traditional Chinese medicine prescriptions were found,with 331 published in Chinese and 3 in English.196567 participants were included,covering 585 types of Chinese patent medicines(487 oral,61 in-jectable,and 37 topical)and 179 classic traditional Chinese medicine prescriptions.The in-volved studies encompassed 22 types of diseases,with research primarily focusing on dis-eases of the circulatory system,the respiratory system,and the genitourinary system.The sample sizes ranged from 18 to 3777 participants,and most studies were conducted at a sin-gle center.Methodologically,the implementation of allocation concealment and blinding re-mained insufficiently emphasized.Conclusion Overall,compared with 2022,both the number of RCT publications and their methodological quality have improved in 2023,with heightened attention to research on diseases of the genitourinary system.However,quality control and standardized management in the design and implementation processes still require enhancement to produce more high-quality clinical evidence and accelerate the translation and application of this evidence.
文摘BACKGROUND Surgically created arterio-venous fistulas(AVFs)are the gold standard for haemodialysis access for patients with end-stage renal disease.Standard practice of AVF creation involves selecting the non-dominant upper limb and starting with most distally with radio-cephalic arterio-venous fistula.The primary patency rate of radio-cephalic arterio-venous fistula varies from 20%-25%.It has been suggested the neointimal hyperplasia at the mobilized venous segment causes stenosis of the anastomosis.Therefore,the radial artery deviation and reimplantation(RADAR)technique,in which the vein is minimally mobilized,should result in a higher success rate.AIM To compare the RADAR technique with classical technique in creation of AVF including:(1)Success rate;(2)Time to maturation;(3)Duration of surgery;and(4)Complication rate.METHODS In our study we recruited 94 patients in two randomized groups and performed the AVF by the classical method or the RADAR method.RESULTS The RADAR group had higher primary success rate(P=0.007),less rate of complications(P=0.04),shorter duration of surgery(P=0.00)and early time to maturation(0.001)when compared with the classical group.The RADAR procedure is a safe and a more efficient alternative to the current classical method of AVF creation.Longer duration of follow-up is required to assess the long-term outcomes in the future.CONCLUSION The RADAR procedure is a safe and more efficient alternative to the current classical method of AVF creation.Longer duration of follow-up is required to assess the long-term outcomes in the future.
文摘The basic human rights concepts in Chinese classics mainly encompass three categories:the concept of order and freedom influenced by the worldview of the harmony between humanity and nature,the concept of human subjectivity and the source of human rights influenced by the people-oriented or benevolence-oriented values,as well as the concept of the relationship between human rights and state power guided by the outlook on life of the great unity of all under heaven(tianxia).Among them,the concept of order and freedom of the harmony between humanity and nature and modeling itself on nature is more beneficial to the stability and development of human society than the Western concept of order and freedom under“rational choice.”The norm prototype of“Do not do to others what you do not want others to do to you”formed the Article 4 of the French Declaration of the Rights of Man and of the Citizen in 1789,and the concepts of“benevolence”and“human superiority”were written into Article 1 of the Universal Declaration of Human Rights and the preamble of the Declaration of the United Nations Conference on the Human Environment,respectively,which have become the common spiritual wealth of all humankind.The ancient Chinese imperial examination system for selecting virtuous and capable people was basically consistent with the provisions on the political rights of citizens to hold public office stipulated in contemporary human rights documents,and was advanced in the world at that time.The contemporary Chinese system of“the state respects and protects human rights”is a system rooted in the Chinese historical and cultural traditions and is with the superiority of socialism.
基金USST Construction Project of English-taught Courses for International Students in 2024Key Course Construction Project in Universities of Shanghai in 2024USST Teaching Achievement Award(postgraduate)Cultivation Project in 2024.
文摘The paper analyzes the English retranslation of The Classic on Tea from the perspective of cultural awareness and finds some misinterpretations and omissions of Chinese culture appeared in the translations of foreign scholars.Domestic translators have a deeper understanding of Chinese culture and their retranslations embody the increasing cultural awareness of the source language culture,mainly in seeking conceptual equivalence rather than lexical equivalence,choosing the most suitable translation according to the textual and cultural context,and focusing on cultural facsimile and readers’awareness.This study will help better spread the Chinese tea culture and help Chinese culture go global.
基金Supported by National College Students Innovative Projects(57315430)~~
文摘With the pilings of rockeries,bridge trails,doors and windows,pavilions,towers,walls,halls and studios,corridors and courtyards as the research subject,illustrated with a large number of pictures,the relationship between architecture and rockery in classical gardens was elaborated.In classical gardens,their harmonious,complementary and positive aspect was emphasized,and their opposite,repulsive and passive aspect was restricted,and even the passiveness could be transferred into positivity,so as to make the beauty of architecture and the beauty of nature coexist,coming to the state that the artificiality and the nature blend into one whole.The research was to provide reference for the design and construction of modern gardens.
