Deep-sea cold seeps form a benthic hypoxic biome characterized by low temperatures,methane venting,and metalliferous fluid emissions.This extreme environment was dominated by high-biomass species such as clams.While m...Deep-sea cold seeps form a benthic hypoxic biome characterized by low temperatures,methane venting,and metalliferous fluid emissions.This extreme environment was dominated by high-biomass species such as clams.While most studies have focused on the symbiotic function of cold seep clams,research on the roles of their non-symbiotic functions remains limited.In this study,we conducted transcriptome analysis to examine gene expression pattern in the testis,adductor muscle(hereinafter“muscle”),and foot of Phreagena okutanii,a clam species collected from the S11 site on the western slope of the Okinawa Trough.Principal component analysis and differential expression analysis revealed that the gene expression patterns of the muscle and foot tissues were shared more similar gene expression patterns with each other than with the testis.A total of 564 co-expressed genes with transcripts per million(TPM)>10 were identified as co-expressed genes in the testis,muscle,and foot.The gene expression patterns of hemoglobin Ⅰ and Ⅱ(hb1,hb2),heat shock proteins 70 and 90(hsp70,hsp90),glutathione peroxidase(GPx),thioredoxin-2(Trx2),and ferritin(fer)for oxygen transportation,stress response and antioxidation were identified in the three tissues.In particular,4853 and 6194 differentially expressed genes(DEGs)were identified in the testis compared to the muscle and foot,respectively,significantly exceeding the 854 DEGs observed between the muscle and foot.Furthermore,DEG intersection enabled the identification of shared tissue-specific expression patterns,including genes that were upregulated in the testis relative to both the muscle and foot;those upregulated in the muscle relative to both the testis and foot,and those upregulated in the foot relative to both the testis and muscle.Antioxidant genes inhibited the production of reactive oxygen species and catalyzed their removal.The immune response gene played a key role in pathogen recognition and elimination.Energy metabolism genes enhanced energy production and accumulation,supporting adaptation to the high-sulfur and low-oxygen environments.Our study provides further insights into the gene expression pattern of bivalves in specialized deep-sea cold seep environments.展开更多
液态金属锂铅包层是最具发展潜力的聚变堆包层之一,其首选结构材料为低活化铁素体/马氏体钢,而它与液态锂铅的相容性是聚变堆材料研究领域的关键问题之一。本文介绍中国低活化马氏体钢CLAM在液态金属锂铅回路DRAGON-1热对流工况下的实...液态金属锂铅包层是最具发展潜力的聚变堆包层之一,其首选结构材料为低活化铁素体/马氏体钢,而它与液态锂铅的相容性是聚变堆材料研究领域的关键问题之一。本文介绍中国低活化马氏体钢CLAM在液态金属锂铅回路DRAGON-1热对流工况下的实验情况及500 h 480℃下初步腐蚀实验结果,并与同样工况下316L奥氏体钢腐蚀结果进行了对比分析。结果显示CLAM钢与液态锂铅的相容性优于316L钢。展开更多
基金The National Natural Science Foundation of China under contract Nos 91858208 and 92358301the Laoshan Laboratory under contract No.LSKJ202203500+2 种基金the China Postdoctoral Science Foundation of China under contract No.2019M663209the Fundamental Research Funds for the Central Universities in China under contract No.19lGPY100the Innovation and Entrepreneurship Training Program for College Students under contract No.202210558122.
文摘Deep-sea cold seeps form a benthic hypoxic biome characterized by low temperatures,methane venting,and metalliferous fluid emissions.This extreme environment was dominated by high-biomass species such as clams.While most studies have focused on the symbiotic function of cold seep clams,research on the roles of their non-symbiotic functions remains limited.In this study,we conducted transcriptome analysis to examine gene expression pattern in the testis,adductor muscle(hereinafter“muscle”),and foot of Phreagena okutanii,a clam species collected from the S11 site on the western slope of the Okinawa Trough.Principal component analysis and differential expression analysis revealed that the gene expression patterns of the muscle and foot tissues were shared more similar gene expression patterns with each other than with the testis.A total of 564 co-expressed genes with transcripts per million(TPM)>10 were identified as co-expressed genes in the testis,muscle,and foot.The gene expression patterns of hemoglobin Ⅰ and Ⅱ(hb1,hb2),heat shock proteins 70 and 90(hsp70,hsp90),glutathione peroxidase(GPx),thioredoxin-2(Trx2),and ferritin(fer)for oxygen transportation,stress response and antioxidation were identified in the three tissues.In particular,4853 and 6194 differentially expressed genes(DEGs)were identified in the testis compared to the muscle and foot,respectively,significantly exceeding the 854 DEGs observed between the muscle and foot.Furthermore,DEG intersection enabled the identification of shared tissue-specific expression patterns,including genes that were upregulated in the testis relative to both the muscle and foot;those upregulated in the muscle relative to both the testis and foot,and those upregulated in the foot relative to both the testis and muscle.Antioxidant genes inhibited the production of reactive oxygen species and catalyzed their removal.The immune response gene played a key role in pathogen recognition and elimination.Energy metabolism genes enhanced energy production and accumulation,supporting adaptation to the high-sulfur and low-oxygen environments.Our study provides further insights into the gene expression pattern of bivalves in specialized deep-sea cold seep environments.
文摘液态金属锂铅包层是最具发展潜力的聚变堆包层之一,其首选结构材料为低活化铁素体/马氏体钢,而它与液态锂铅的相容性是聚变堆材料研究领域的关键问题之一。本文介绍中国低活化马氏体钢CLAM在液态金属锂铅回路DRAGON-1热对流工况下的实验情况及500 h 480℃下初步腐蚀实验结果,并与同样工况下316L奥氏体钢腐蚀结果进行了对比分析。结果显示CLAM钢与液态锂铅的相容性优于316L钢。
