Vacuolar protein sorting 36(VPS36),a protein primarily known for its role in the Endosomal Sorting Complex Required for Transport pathway,has recently been shown to be linked to chicken reproduction.Previous research ...Vacuolar protein sorting 36(VPS36),a protein primarily known for its role in the Endosomal Sorting Complex Required for Transport pathway,has recently been shown to be linked to chicken reproduction.Previous research showed that Vps36 is significantly downregulated in sexually mature chicken ovaries compared to immature ones.In this study,using real-time quantitative RT-PCR,we investigated the expression pattern of Vps36 and its head-to-head gene Ckap2 mRNA in chicken follicles.Small white follicles were found to have significantly higher expression of Vps36 and Ckap2 mRNA than any other sized follicles(P<0.05).The expression of Vps36 and Ckap2 mRNA were detected in both granulosa and theca layers of pre-ovulatory follicles,the expression of Ckap2 in theca layers was slightly higher than in granulosa cells.Treatment of small yellow follicles with folliclestimulating hormone and estradiol resulted in a marked decrease of both Vps36 and Ckap2 mRNA(P<0.05);however,progesterone,transforming growth factor-β1 and luteinizing hormone induced no significant changes in Vps36 and Ckap2 mRNA expression in these follicles.These results indicate that the head-to-head genes of Vps36 and Ckap2 exhibit similar expression in chicken follicles and are involved in chicken follicle development.展开更多
背景与目的 细胞骨架相关蛋白2L(cytoskeleton associated protein 2 like protein,CKAP2L)是一种对神经干细胞或祖细胞很重要的有丝分裂纺锤体蛋白,参与多种恶性肿瘤的生物学行为。本研究探讨CKAP2L基因在不同肿瘤中的表达情况,及与患...背景与目的 细胞骨架相关蛋白2L(cytoskeleton associated protein 2 like protein,CKAP2L)是一种对神经干细胞或祖细胞很重要的有丝分裂纺锤体蛋白,参与多种恶性肿瘤的生物学行为。本研究探讨CKAP2L基因在不同肿瘤中的表达情况,及与患者预后和免疫细胞浸润的相关性。方法 通过TIMER、TCGA及GTEx数据库分析CKAP2L在33种肿瘤及正常组织的差异表达情况。利用Kaplan-Meier生存分析研究在不同肿瘤中CKAP2L表达水平与患者预后的相关性。利用Wilcoxon秩和检验分析不同肿瘤中CKAP2L表达与临床病理分期的相关性。采用Spearman相关性分析研究CKAP2L表达与肿瘤突变负荷(tumor mutation burden,TMB)、微卫星不稳定性(microsatelliteinstability,MSI)、免疫细胞浸润及免疫检查点之间的相关性。结果 TIMER数据库显示,CKAP2L在20种肿瘤中高表达。在肾上腺皮质癌、肾乳头状透明细胞癌、脑低级别胶质瘤、肝细胞肝癌、肺腺癌、间皮瘤、胰腺癌、黑色素瘤和子宫内膜癌中,CP2L高表达的患者预后较差;而在胸腺癌中,CP2L高表达的患者预后较好。在肾上腺皮质癌、肾透明细胞癌、肾乳头状透明细胞癌、肝细胞肝癌和肺腺癌中,CP2L表达与不同病理分期相关。在肺鳞状细胞癌、结肠癌、胃腺癌和肉瘤中,CP2L表达TMB和MSI呈正相关。CP2L表达与一些肿瘤中的免疫细胞浸润和免疫检查点也密切相关。结论 CP2L可能成为预测肿瘤预后的潜在生物标志物,为临床诊疗及精准化治疗提供参考。展开更多
基金This work was funded by the National Natural Science Foundation of China(30871777)Platform Construction of Genetic Resources of Livestock and Poultry Breeds in China(2005DKA21101)Agricultural Elite Breeds(Poultry)Project of Shandong Province(2009LZ09-03).
文摘Vacuolar protein sorting 36(VPS36),a protein primarily known for its role in the Endosomal Sorting Complex Required for Transport pathway,has recently been shown to be linked to chicken reproduction.Previous research showed that Vps36 is significantly downregulated in sexually mature chicken ovaries compared to immature ones.In this study,using real-time quantitative RT-PCR,we investigated the expression pattern of Vps36 and its head-to-head gene Ckap2 mRNA in chicken follicles.Small white follicles were found to have significantly higher expression of Vps36 and Ckap2 mRNA than any other sized follicles(P<0.05).The expression of Vps36 and Ckap2 mRNA were detected in both granulosa and theca layers of pre-ovulatory follicles,the expression of Ckap2 in theca layers was slightly higher than in granulosa cells.Treatment of small yellow follicles with folliclestimulating hormone and estradiol resulted in a marked decrease of both Vps36 and Ckap2 mRNA(P<0.05);however,progesterone,transforming growth factor-β1 and luteinizing hormone induced no significant changes in Vps36 and Ckap2 mRNA expression in these follicles.These results indicate that the head-to-head genes of Vps36 and Ckap2 exhibit similar expression in chicken follicles and are involved in chicken follicle development.
