Alzheimer's disease,a progressively degenerative neurological disorder,is the most common cause of dementia in the elderly.While its precise etiology remains unclear,researchers have identified diverse pathologica...Alzheimer's disease,a progressively degenerative neurological disorder,is the most common cause of dementia in the elderly.While its precise etiology remains unclear,researchers have identified diverse pathological characteristics and molecular pathways associated with its progression.Advances in scientific research have increasingly highlighted the crucial role of non-coding RNAs in the progression of Alzheimer's disease.These non-coding RNAs regulate several biological processes critical to the advancement of the disease,offering promising potential as therapeutic targets and diagnostic biomarkers.Therefore,this review aims to investigate the underlying mechanisms of Alzheimer's disease onset,with a particular focus on microRNAs,long non-coding RNAs,and circular RNAs associated with the disease.The review elucidates the potential pathogenic processes of Alzheimer's disease and provides a detailed description of the synthesis mechanisms of the three aforementioned non-coding RNAs.It comprehensively summarizes the various non-coding RNAs that have been identified to play key regulatory roles in Alzheimer's disease,as well as how these noncoding RNAs influence the disease's progression by regulating gene expression and protein functions.For example,miR-9 targets the UBE4B gene,promoting autophagy-mediated degradation of Tau protein,thereby reducing Tau accumulation and delaying Alzheimer's disease progression.Conversely,the long non-coding RNA BACE1-AS stabilizes BACE1 mRNA,promoting the generation of amyloid-βand accelerating Alzheimer's disease development.Additionally,circular RNAs play significant roles in regulating neuroinflammatory responses.By integrating insights from these regulatory mechanisms,there is potential to discover new therapeutic targets and potential biomarkers for early detection and management of Alzheimer's disease.This review aims to enhance the understanding of the relationship between Alzheimer's disease and non-coding RNAs,potentially paving the way for early detection and novel treatment strategies.展开更多
Graphite,encompassing both natural graphite and synthetic graphite,and graphene,have been extensively utilized and investigated as anode materials and additives in lithium-ion batteries(LIBs).In the pursuit of carbon ...Graphite,encompassing both natural graphite and synthetic graphite,and graphene,have been extensively utilized and investigated as anode materials and additives in lithium-ion batteries(LIBs).In the pursuit of carbon neutrality,LIBs are expected to play a pivotal role in reducing CO_(2)emissions by decreasing reliance on fossil fuels and enabling the integration of renewable energy sources.Owing to their technological maturity and exceptional electrochemical performance,the global production of graphite and graphene for LIBs is projected to continue expanding.Over the past decades,numerous researchers have concentrated on reducing the material and energy input whilst optimising the electrochemical performance of graphite and graphene,through novel synthesis methods and various modifications at the laboratory scale.This review provides a comprehensive examination of the manufacturing methods,environmental impact,research progress,and challenges associated with graphite and graphene in LIBs from an industrial perspective,with a particular focus on the carbon footprint of production processes.Additionally,it considers emerging challenges and future development directions of graphite and graphene,offering significant insights for ongoing and future research in the field of green LIBs.展开更多
Brazil maintains a leading position in agricultural exports and stands as the world's foremost producer and user of bioinputs in agriculture.These bioinputs generate annual savings of billions of dollars that woul...Brazil maintains a leading position in agricultural exports and stands as the world's foremost producer and user of bioinputs in agriculture.These bioinputs generate annual savings of billions of dollars that would otherwise be allocated to chemical fertilizers and pesticides.The nation's regulatory framework enables bioinput agriculture and serves as a model for countries transitioning toward regenerative agriculture.Brazilian legislation categorizes bioinputs into:1)biofertilizers(extracts);2)biostimulants(plant growth-promoting and biocontrol agents);and 3)inoculants(active ingredient comprises one or more living microorganisms).The inoculation of soybeans with Bradyrhizobium strains provides approximately 90%of the nitrogen accumulated by this crop.Brazil has registered over six hundred inoculants,with at least 60%specifically designated for soybean cultivation.The annual sales of inoculants in Brazil reach approximately 120 million doses.Although beans(Phaseolus vulgaris and Vigna unguiculata)represent an essential food crop in Brazil's staple diet and benefit from inoculation,inoculant supply remains insufficient.Regarding biocontrol,soy,corn,sugarcane,and coffee rank among the most protected crops,employing biocontrol agents against bacteria,fungi,nematodes,and insects.Bacillus,Pseudomonas,Streptomyces,Rhizobium,Azotobacter,and Paenibacillus strains were predominantly cited in the 5,000+bioproduct patents filed between 2022 and 2024.Among fungal genera,Trichoderma,and Penicillium received the most citations.EMBRAPA's biobanks maintain over 10,000 strains of bacteria,fungi,and viruses for biocontrol,and 14,000 strains of nutrient-fixing and plant-growth promoters.Production challenges include quality control,particularly as on-farm production of inoculants becomes prevalent on larger farms,alongside product availability and supply limitations.Brazilian farmers maintain global competitiveness partly through reduced chemical fertilizer and pesticide costs enabled by bioinput usage.As components of regenerative agriculture,bioinputs enhance soil quality,decrease carbon footprints,and support Sustainable Development Goals.Brazil's leadership in microbial bioinput utilization stems from its extensive agricultural sector,rich microbial biodiversity,and progressive regulatory framework.展开更多
The recovery of precious metals(PMs)from secondary resources is critical for addressing global supply-chain vulnerabilities and sustainable resource utilization.This review systematically examines the transformative p...The recovery of precious metals(PMs)from secondary resources is critical for addressing global supply-chain vulnerabilities and sustainable resource utilization.This review systematically examines the transformative potential of metal-organic frameworks(MOFs)as next-generation adsorbents for PM recovery,focusing on their synthesis,functionalization,and multiscale adsorption mechanisms.We critically analyze conventional pyrometallurgical and hydrometallurgical methods and highlight their limitations in terms of selectivity,energy consumption,and secondary pollution.In contrast,MOFs offer tunable porosity,abundant active sites,and tunable surface chemistry,enabling efficient PM capture via synergistic physical and chemical adsorption.Advanced modification techniques,including direct synthesis and post-synthetic modification,are reviewed to propose strategies for enhancing the adsorption kinetics and selectivity for Au,Ag,Pt,and Pd.Key structure-property relationships are established through multiscale characterization and thermodynamic models,revealing the critical roles of hierarchical porosity,soft donor atoms,and framework stability.Industrial challenges,such as aqueous stability and scalability,are addressed via Zr-O bond strengthening,hydrophobic functionalization,and support immobilization.This study consolidates the experimental and theoretical advances in MOF-based PM recovery and provides a roadmap for translating laboratory innovations into practical applications within the circular-economy framework.展开更多
Stroke-induced alterations in cerebral blood flow trigger neurovascular remodeling,as manifested by the blood-brain barrier dysfunction and subs equent neurovascular repair activities such as angiogenesis.This process...Stroke-induced alterations in cerebral blood flow trigger neurovascular remodeling,as manifested by the blood-brain barrier dysfunction and subs equent neurovascular repair activities such as angiogenesis.This process involves neurovascular communication that facilitates the transport of mediators among cerebrovascular endothelial cells,pericytes,glial cells,and neurons,thereby transmitting signals from donor to recipient cells to elicit a collaborative response.展开更多
Owing to their intricate molecular frameworks and copious chiral centers,the structural identification and configurational assignment of natural products are challenging tasks.Comprehensive spectral data analysis is c...Owing to their intricate molecular frameworks and copious chiral centers,the structural identification and configurational assignment of natural products are challenging tasks.Comprehensive spectral data analysis is crucial for the confirmation of absolute configurations.Ignoring critical parameters will lead to false structure,which may confuse the total synthesis and drug development.Herein,the configurations of seven heterogeneous Pallavicinia diterpenoids(PDs) isolated from Pallavicinia liverworts are revised using a combination of single-crystal X-ray diffraction and electronic circular dichroism(ECD) calculations.Meanwhile,identification of five unprecedented PD heterodimers PD-dimers A-E(18-22) along with eleven previously undescribed PDs(5-9,13-17,23) obtained by the reinvestigation of the Chinese liverwort Pallavicinia subciliata have resulted in corrections and support the revised conclusions.展开更多
Laser welding is a highly promising joining method for Al alloys.However,certain limitations such as elevated thermal input and keyhole instability are associated with its application in medium-thickness aluminium all...Laser welding is a highly promising joining method for Al alloys.However,certain limitations such as elevated thermal input and keyhole instability are associated with its application in medium-thickness aluminium alloy plates.To address these issues,circular oscillating laser welding combined with post-weld heat treatment was employed to improve the formation quality and mechanical properties of the welds.The effects of the frequency of circular oscillating laser on the forming quality,microstructure,and properties of the welds were analyzed.At an oscillation frequency of 200 Hz,the grain size in the weld zone was reduced compared to single laser welding,and the maximum tensile strength of the weld was observed to reach(264.96±1.33)MPa,representing approximately 61.19%of the base metal.Following the post-weld heat treatment of"solid solution and artificial aging",the grain boundary segregation was diminished.Nanoscale precipitated phases are present in the weld zone.Furthermore,the tensile strength was augmented to(386.35±5.65)MPa,representing approximately 89.23%of the strength of the base metal.The results of this study can provide a theoretical basis and technological reference for the circular oscillating laser welding of medium-thickness 2219-T 6 aluminium alloy plates.展开更多
Acute ischemic stroke is a clinical emergency and a condition with high morbidity,mortality,and disability.Accurate predictive,diagnostic,and prognostic biomarkers and effective therapeutic targets for acute ischemic ...Acute ischemic stroke is a clinical emergency and a condition with high morbidity,mortality,and disability.Accurate predictive,diagnostic,and prognostic biomarkers and effective therapeutic targets for acute ischemic stroke remain undetermined.With innovations in high-throughput gene sequencing analysis,many aberrantly expressed non-coding RNAs(ncRNAs)in the brain and peripheral blood after acute ischemic stroke have been found in clinical samples and experimental models.Differentially expressed ncRNAs in the post-stroke brain were demonstrated to play vital roles in pathological processes,leading to neuroprotection or deterioration,thus ncRNAs can serve as therapeutic targets in acute ischemic stroke.Moreover,distinctly expressed ncRNAs in the peripheral blood can be used as biomarkers for acute ischemic stroke prediction,diagnosis,and prognosis.In particular,ncRNAs in peripheral immune cells were recently shown to be involved in the peripheral and brain immune response after acute ischemic stroke.In this review,we consolidate the latest progress of research into the roles of ncRNAs(microRNAs,long ncRNAs,and circular RNAs)in the pathological processes of acute ischemic stroke–induced brain damage,as well as the potential of these ncRNAs to act as biomarkers for acute ischemic stroke prediction,diagnosis,and prognosis.Findings from this review will provide novel ideas for the clinical application of ncRNAs in acute ischemic stroke.展开更多
A tunable oxidization and reduction strategy was proposed to directly regenerate spent LiFePO_(4)/C cathode materials by oxidizing excessive carbon powders with the addition of FePO_(4).Experimental results indicate t...A tunable oxidization and reduction strategy was proposed to directly regenerate spent LiFePO_(4)/C cathode materials by oxidizing excessive carbon powders with the addition of FePO_(4).Experimental results indicate that spent LiFePO_(4)/C cathode materials with good performance can be regenerated by roasting at 650℃ for 11 h with the addition ofLi_(2)CO_(3),FePO_(4),V_(2)O_(5),and glucose.V_(2)O_(5) is added to improve the cycle performance of regenerated cathode materials.Glucose is used to revitalize the carbon layers on the surface of spent LiFePO_(4)/C particles for improving their conductivity.The regenerated V-doped LiFePO_(4)/C shows an excellent electrochemical performance with the discharge specific capacity of 161.36 mA·h/g at 0.2C,under which the capacity retention is 97.85%after 100 cycles.展开更多
The rapid growth of the global population,coupled with increasing pollution levels,highlights the urgent need for sustainable and eco-friendly construction materials,such as unfired clay bricks.However,their widesprea...The rapid growth of the global population,coupled with increasing pollution levels,highlights the urgent need for sustainable and eco-friendly construction materials,such as unfired clay bricks.However,their widespread adoption remains limited due to certain performance drawbacks,particularly in thermal insulation,a critical factor in addressing climate change challenges.In this study,a plant-based waste-derived biopolymer was incorporated into unfired clay bricks to enhance their physicochemical and thermomechanical properties.The biopolymer was added at six different weight fractions(0%,1%,3%,7%,15%,and 20%)to systematically evaluate its impact on bulk density,porosity,capillary water absorption,thermal conductivity,specific heat capacity,and compressive strength.The results revealed a gradual decrease in porosity as the biopolymer content increased,leading to a 41%improvement in thermal conductivity at 20 wt%.However,the optimal balance between thermal efficiency and compressive strength was achieved at 7 wt%biopolymer;this result has been verified through a combination of experimental methods and modeling.Additionally,TRNSYS simulations confirmed the enhanced thermal performance,demonstrating a 9.74%increase in time lag and a 16%reduction in decrement factor,both of which contribute to optimizing building energy efficiency.Overall,this approach not only helps reduce environmental pollution but also enhances insulation capacity while lowering heating and cooling demands,thereby improving overall building performance.Biopolymer-reinforced unfired clay bricks thus represent a promising solution for advancing a low-carbon and sustainable construction industry,aligning with the United Nations Sustainable Development Goals(SDGs)for climate change mitigation and responsible resource management.展开更多
With the continuous increase in the disposal volume of spent lithium-ion batteries(LIBs),properly recycling spent LIBs has become essential for the advancement of the circular economy.This study presents a systematic ...With the continuous increase in the disposal volume of spent lithium-ion batteries(LIBs),properly recycling spent LIBs has become essential for the advancement of the circular economy.This study presents a systematic analysis of the chlorination roasting kinetics and proposes a new two-step chlorination roasting process that integrates thermodynamics for the recycling of LIB cathode materials.The activation energy for the chloride reaction was 88.41 kJ/mol according to thermogravimetric analysis–derivative thermogravimetry data obtained by using model-free,model-fitting,and Z(α)function(αis conversion rate).Results indicated that the reaction was dominated by the first-order(F1)model when the conversion rate was less than or equal to 0.5 and shifted to the second-order(F2)model when the conversion rate exceeded 0.5.Optimal conditions were determined by thoroughly investigating the effects of roasting temperature,roasting time,and the mass ratio of NH_(4)Cl to LiCoO_(2).Under the optimal conditions,namely 400℃,20 min,and NH_(4)Cl/LiCoO_(2)mass ratio of 3:1,the leaching efficiency of Li and Co reached 99.43% and 99.05%,respectively.Analysis of the roasted products revealed that valuable metals in LiCoO_(2)transformed into CoCl_(2) and LiCl.Furthermore,the reaction mechanism was elucidated,providing insights for the establishment of a novel low-temperature chlorination roasting technology based on a crystal structure perspective.This technology can guide the development of LIB recycling processes with low energy consumption,low secondary pollution,high recovery efficiency,and high added value.展开更多
Multiple functional metasurfaces with high information capacity have attracted considerable attention from researchers.This study proposes a 2-bit tunable spin-decoupled coded metasurface designed for the terahertz ba...Multiple functional metasurfaces with high information capacity have attracted considerable attention from researchers.This study proposes a 2-bit tunable spin-decoupled coded metasurface designed for the terahertz band,which utilizes the tunable properties of Dirac semimetals(DSM)to create a novel multilayer structure.By incorporating both geometric and propagating phases into the metasurface design,we can effectively control the electromagnetic wave.When the Fermi level(EF)of the DSM is set at 6 meV,the electromagnetic wave is manipulated by the gold patch embedded in the DSM film,operating at a frequency of 1.3 THz.When the EF of the DSM is set at 80 meV,the electromagnetic wave is manipulated by the DSM patch,operating at a frequency of 1.4 THz.Both modes enable independent control of beam splitting under left-rotating circularly polarized(LCP)and rightrotating circularly polarized(RCP)wave excitation,resulting in the generation of vortex beams with distinct orbital angular momentum(OAM)modes.The findings of this study hold significant potential for enhancing information capacity and polarization multiplexing techniques in wireless communications.展开更多
The development of sustainable sludge management systems requires looking at them with a new vision in which the concepts of SD(Sustainable Development)must integrate those of CE(Circular Economy),both concepts subjec...The development of sustainable sludge management systems requires looking at them with a new vision in which the concepts of SD(Sustainable Development)must integrate those of CE(Circular Economy),both concepts subject to the principles of TD(Thermodynamics),thus allowing the adoption of actions that are all the more effective the more complete the evaluation of the social dimension has been.This involves a new“Way of thinking”which sees the sludge system as the“Locomotive”of the entire wastewater/sludge treatment train and is developed through“Ways of acting”which includes both“Technical”actions to maximize recoveries of useful materials and/or or energy,and“Socio/Institutional”actions to overcome barriers linked to local cultures and traditions,also considering that the specific local context heavily influences the choices capable of satisfying the concepts of CE.It follows the need of issuing realistic and applicable regulations and overcoming social barriers,such as lack of infrastructure and/or qualified personnel,to achieve an effective integration of the concepts of CE with the more general ones of sustainability.展开更多
LEADSFON(XIAMEN)TEXTILE TECH CO.,LTD.is a manufacturer of knitting circular machines.Since 2002,the company has served as an ODM and supporting partner for the Italian brand"PILOTELLI".In 2014,LEADSFON offic...LEADSFON(XIAMEN)TEXTILE TECH CO.,LTD.is a manufacturer of knitting circular machines.Since 2002,the company has served as an ODM and supporting partner for the Italian brand"PILOTELLI".In 2014,LEADSFON officially acquired PILOTELLI,integrating advanced Italian technology into its core operations.展开更多
BACKGROUND:Circulating biomarkers for sepsis are lacking,and research on circular RNAs(circR NAs)as potential biomarkers of pneumonia-induced sepsis is limited.This study aims to investigate the diagnostic and prognos...BACKGROUND:Circulating biomarkers for sepsis are lacking,and research on circular RNAs(circR NAs)as potential biomarkers of pneumonia-induced sepsis is limited.This study aims to investigate the diagnostic and prognostic potential of circRNAs in patients with pneumonia-induced sepsis.METHODS:This prospective cohort study included 40 healthy individuals,60 patients with pneumonia,and 80 patients with pneumonia-induced sepsis.CircRNAs identified through RNA-sequencing were validated using quantitative real-time polymerase chain reaction(qRT-PCR).Spearman correlation analysis was used to evaluate the associations between circRNAs,inflammatory markers,Sequential Organ Failure Assessment(SOFA)scores,and Acute Physiology and Chronic Health EvaluationⅡ(APACHEⅡ)scores.Receiver operating characteristic(ROC)curves analysis were used to assess the diagnostic performance of circRNAs,while ROC curves and Kaplan-Meier survival analysis were used to evaluate their prognostic value of 28-day mortality.RESULTS:qRT-PCR confirmed the significant upregulation of Circ-CTD-2281E23.2 and downregulation of Circ-0075723 and Circ-0008679 in sepsis patients.Spearman correlation analysis showed that Circ-CTD-2281E23.2 was positively correlated with inflammatory markers and severity scores,whereas Circ-0075723 and Circ-0008679 were negatively correlated with these parameters.The area under the curve(AUC)values for Circ-CTD-2281E23.2,Circ-0075723,and Circ-0008679 in diagnosing pneumonia-induced sepsis were 0.728,0.706,and 0.793,respectively.The combination of these circRNAs(AUC=0.846)and the combination with other clinical indicators(AUC=0.990)demostrated enhanced AUC values.The AUC values for Circ-CTD-2281E23.2 and Circ-0075723 in predicting 28-day mortality were 0.664 and 0.765,respectively.CONCLUSION:This study suggest the additional diagnostic and prognostic value of circRNAs in pneumonia-induced sepsis.Circ-CTD-2281E23.2,Circ-0075723,and Circ-0008679 exhibit diagnostic potential,with Circ-CTD-2281E23.2 and Circ-0075723 showing positive prognostic value for 28-day mortality in sepsis patients.展开更多
In this comprehensive review,the evolution and progress of bioplastics are examined,with an emphasis on their types,production methods,environmental impact,and biodegradability.In light of the increasing global effort...In this comprehensive review,the evolution and progress of bioplastics are examined,with an emphasis on their types,production methods,environmental impact,and biodegradability.In light of the increasing global efforts to address environmental degradation,bioplastics have emerged as a highly potential substitute for conventional petroleum-based plastics.This review classifies various categories of bioplastics,encompassing both biodegradable and bio-based variations,and assesses their environmental consequences using life cycle evaluations and biodegradability calculations.This paper analyzes the technological advancements that have enhanced the mechanical and thermal characteristics of bioplastics,hence increasing their feasibility for extensive commercial applications in diverse sectors.This review critically examines the possible uses of bioplastics in important industries including packaging,aerospace,and healthcare,emphasizing both achievements and current obstacles.In addition,the assessment addresses the economic and technical obstacles to expanding bioplastic manufacturing,namely concerns about cost,material efficiency,and waste disposal.Moreover,the article forecasts the future potential of bioplastics in furthering a sustainable circular economy and suggests methods to address existing constraints,such as improvements in recycling technology and the establishment of more economically efficient manufacturing methods.The findings are intended to educate policymakers,industry stakeholders,and researchers on the crucial contribution of bioplastics in attaining sustainability objectives and promoting innovation in the field of material science.展开更多
Layered rock formations are frequently encountered during the excavation of underground structures. The stability of such structures is influenced not only by the stress concentration caused by the cavities in the str...Layered rock formations are frequently encountered during the excavation of underground structures. The stability of such structures is influenced not only by the stress concentration caused by the cavities in the strata but also by the anisotropy of the layered rock mass. The interaction between them can lead to critical structural failure, such as rupture, collapse, or significant deformation within the adjacent rock mass, thereby jeopardizing operational safety. However, the coupling law and mechanism between the stress concentration resulting from the cavities and the anisotropy of a layered rock mass remain unclear. In this study, a uniaxial compression test was performed on shale specimens containing a circular hole to investigate the effects of layer inclination and circular holes on the mechanical properties, elastic energy storage, and failure behaviors of these specimens. The failure mechanism of the rock surrounding the hole was analyzed on the basis of the single plane of weakness theory and the Kirsch solution. The test results indicated pronounced anisotropy in the compressive strength, elastic modulus, and elastic strain energy of the specimens, with distinct “V”, “M” and “U”-shaped patterns correlated with varying layer inclination angles. In addition, the combined effect of stress concentration and layer inclination resulted in different failure types, which were classified into four groups according to their failure behavior. Theoretical analysis revealed that failure around circular holes in layered rock is affected by a range of variables, such as layer inclination, layer strength, lateral pressure coefficient, azimuth, and loading stress.展开更多
BACKGROUND Myocardial ischemia/reperfusion(I/R)injury,which is associated with high morbidity and mortality,is a main cause of unexpected myocardial injury after acute myocardial infarction.However,the underlying mech...BACKGROUND Myocardial ischemia/reperfusion(I/R)injury,which is associated with high morbidity and mortality,is a main cause of unexpected myocardial injury after acute myocardial infarction.However,the underlying mechanism remains unclear.Circular RNAs(circRNAs),which are formed from protein-coding genes,can sequester microRNAs or proteins,modulate transcription and interfere with splicing.Authoritative studies suggest that circRNAs may play an important role in myocardial I/R injury.AIM To explore the role and mechanism of circRNAs in myocardial I/R injury.METHODS We constructed a myocardial I/R injury model using ligation of the left anterior descending coronary artery,and evaluated the success of the validated model using triphenyltetrazolium chloride and hematoxylin-eosin staining.Then,left ventricular samples from different groups were selected for mRNA-sequence,and differential gene screening was performed on the obtained results.The differentially obtained mRNAs were divided into up-regulated and down-regulated according to their expression levels,and Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)functional enrichment analysis were performed,respectively.Then,the obtained circRNA and microRNA(miRNA)were paired for analysis,and the binding sites of miRNA and mRNA were virtual screened.Finally,the obtained circRNA,miRNA and mRNA were constructed by ceRNA mutual most useful network.RESULTS We used an RNA sequencing array to investigate the expression signatures of circRNAs in myocardial I/R injury using three samples from the I/R group and three samples from the sham group.A total of 142 upregulated and 121 downregulated circRNAs were found to be differentially expressed(fold change≥2,P<0.05).GO and KEGG functional analyses of these circRNAs were performed.GO analysis revealed that these circRNAs were involved mainly in cellular and intracellular processes.KEGG analysis demonstrated that 6 of the top 20 pathways were correlated with cell apoptosis.Furthermore,a circRNA-miRNA coexpression network and ceRNA network based on these genes were constructed,revealing that mmu-circ-0001452,mmu-circ-0001637,and mmu-circ-0000870 might be key regulators of myocardial I/R injury.CONCLUSION This research provides new insights into the mechanism of myocardial I/R,which mmu-circ-0001452,mmu-circ-0001637,and mmu-circ-0000870 are expected to be new therapeutic targets for myocardial I/R injury.展开更多
文摘Alzheimer's disease,a progressively degenerative neurological disorder,is the most common cause of dementia in the elderly.While its precise etiology remains unclear,researchers have identified diverse pathological characteristics and molecular pathways associated with its progression.Advances in scientific research have increasingly highlighted the crucial role of non-coding RNAs in the progression of Alzheimer's disease.These non-coding RNAs regulate several biological processes critical to the advancement of the disease,offering promising potential as therapeutic targets and diagnostic biomarkers.Therefore,this review aims to investigate the underlying mechanisms of Alzheimer's disease onset,with a particular focus on microRNAs,long non-coding RNAs,and circular RNAs associated with the disease.The review elucidates the potential pathogenic processes of Alzheimer's disease and provides a detailed description of the synthesis mechanisms of the three aforementioned non-coding RNAs.It comprehensively summarizes the various non-coding RNAs that have been identified to play key regulatory roles in Alzheimer's disease,as well as how these noncoding RNAs influence the disease's progression by regulating gene expression and protein functions.For example,miR-9 targets the UBE4B gene,promoting autophagy-mediated degradation of Tau protein,thereby reducing Tau accumulation and delaying Alzheimer's disease progression.Conversely,the long non-coding RNA BACE1-AS stabilizes BACE1 mRNA,promoting the generation of amyloid-βand accelerating Alzheimer's disease development.Additionally,circular RNAs play significant roles in regulating neuroinflammatory responses.By integrating insights from these regulatory mechanisms,there is potential to discover new therapeutic targets and potential biomarkers for early detection and management of Alzheimer's disease.This review aims to enhance the understanding of the relationship between Alzheimer's disease and non-coding RNAs,potentially paving the way for early detection and novel treatment strategies.
基金supported by European Union's Horizon Europe,UK Research and Innovation(UKRI).
文摘Graphite,encompassing both natural graphite and synthetic graphite,and graphene,have been extensively utilized and investigated as anode materials and additives in lithium-ion batteries(LIBs).In the pursuit of carbon neutrality,LIBs are expected to play a pivotal role in reducing CO_(2)emissions by decreasing reliance on fossil fuels and enabling the integration of renewable energy sources.Owing to their technological maturity and exceptional electrochemical performance,the global production of graphite and graphene for LIBs is projected to continue expanding.Over the past decades,numerous researchers have concentrated on reducing the material and energy input whilst optimising the electrochemical performance of graphite and graphene,through novel synthesis methods and various modifications at the laboratory scale.This review provides a comprehensive examination of the manufacturing methods,environmental impact,research progress,and challenges associated with graphite and graphene in LIBs from an industrial perspective,with a particular focus on the carbon footprint of production processes.Additionally,it considers emerging challenges and future development directions of graphite and graphene,offering significant insights for ongoing and future research in the field of green LIBs.
基金funded in part by the Postgraduate Program of the Paulo de Góes Institute of Microbiology,Federal University of Rio de Janeiro(UFRJ),through the coordination of Higher Education Personnel Improvement(CAPES)(001),Brazilthe National Council for Scientific and Technological Development of Brazil(MCTI-CNPq)(309461/2019-7)+3 种基金the Rio de Janeiro State Research Support Foundation(FAPERJ),E26/200428/2023,Brazilthe support of Embrapa Agrobiology,the State Secretariat for Economic Development,Industry,Trade,and Services(SEDEICS),Brazilthe National Association for the Promotion and Innovation of the Biological Industry(ANPII-BIO)the CropLife biological products,and the Brazilian National Institute of Industrial Property(INPI)。
文摘Brazil maintains a leading position in agricultural exports and stands as the world's foremost producer and user of bioinputs in agriculture.These bioinputs generate annual savings of billions of dollars that would otherwise be allocated to chemical fertilizers and pesticides.The nation's regulatory framework enables bioinput agriculture and serves as a model for countries transitioning toward regenerative agriculture.Brazilian legislation categorizes bioinputs into:1)biofertilizers(extracts);2)biostimulants(plant growth-promoting and biocontrol agents);and 3)inoculants(active ingredient comprises one or more living microorganisms).The inoculation of soybeans with Bradyrhizobium strains provides approximately 90%of the nitrogen accumulated by this crop.Brazil has registered over six hundred inoculants,with at least 60%specifically designated for soybean cultivation.The annual sales of inoculants in Brazil reach approximately 120 million doses.Although beans(Phaseolus vulgaris and Vigna unguiculata)represent an essential food crop in Brazil's staple diet and benefit from inoculation,inoculant supply remains insufficient.Regarding biocontrol,soy,corn,sugarcane,and coffee rank among the most protected crops,employing biocontrol agents against bacteria,fungi,nematodes,and insects.Bacillus,Pseudomonas,Streptomyces,Rhizobium,Azotobacter,and Paenibacillus strains were predominantly cited in the 5,000+bioproduct patents filed between 2022 and 2024.Among fungal genera,Trichoderma,and Penicillium received the most citations.EMBRAPA's biobanks maintain over 10,000 strains of bacteria,fungi,and viruses for biocontrol,and 14,000 strains of nutrient-fixing and plant-growth promoters.Production challenges include quality control,particularly as on-farm production of inoculants becomes prevalent on larger farms,alongside product availability and supply limitations.Brazilian farmers maintain global competitiveness partly through reduced chemical fertilizer and pesticide costs enabled by bioinput usage.As components of regenerative agriculture,bioinputs enhance soil quality,decrease carbon footprints,and support Sustainable Development Goals.Brazil's leadership in microbial bioinput utilization stems from its extensive agricultural sector,rich microbial biodiversity,and progressive regulatory framework.
基金supported by the National Natural Science Foundation of China(No.52304329)the Yunnan Fundamental Research Projects(No.202201BE070001-003),Guo Lin would like to acknowledge Xing Dian talent support program of Yunnan Province.
文摘The recovery of precious metals(PMs)from secondary resources is critical for addressing global supply-chain vulnerabilities and sustainable resource utilization.This review systematically examines the transformative potential of metal-organic frameworks(MOFs)as next-generation adsorbents for PM recovery,focusing on their synthesis,functionalization,and multiscale adsorption mechanisms.We critically analyze conventional pyrometallurgical and hydrometallurgical methods and highlight their limitations in terms of selectivity,energy consumption,and secondary pollution.In contrast,MOFs offer tunable porosity,abundant active sites,and tunable surface chemistry,enabling efficient PM capture via synergistic physical and chemical adsorption.Advanced modification techniques,including direct synthesis and post-synthetic modification,are reviewed to propose strategies for enhancing the adsorption kinetics and selectivity for Au,Ag,Pt,and Pd.Key structure-property relationships are established through multiscale characterization and thermodynamic models,revealing the critical roles of hierarchical porosity,soft donor atoms,and framework stability.Industrial challenges,such as aqueous stability and scalability,are addressed via Zr-O bond strengthening,hydrophobic functionalization,and support immobilization.This study consolidates the experimental and theoretical advances in MOF-based PM recovery and provides a roadmap for translating laboratory innovations into practical applications within the circular-economy framework.
基金supported by the National Natural Science Foundation of China,Nos.82171344(to ZY),82471313(to CKT)the Guangdong Basic and Applied Basic Research Foundation,China,Nos.2023B1515120035,2024A1515012035(to CKT)The Science and Technology Projects in Guangzhou Nos.2025A03J4169(to ZY)。
文摘Stroke-induced alterations in cerebral blood flow trigger neurovascular remodeling,as manifested by the blood-brain barrier dysfunction and subs equent neurovascular repair activities such as angiogenesis.This process involves neurovascular communication that facilitates the transport of mediators among cerebrovascular endothelial cells,pericytes,glial cells,and neurons,thereby transmitting signals from donor to recipient cells to elicit a collaborative response.
基金supported by the National Natural Science Foundation of China (Nos.82293682,82293684,and 82173703)。
文摘Owing to their intricate molecular frameworks and copious chiral centers,the structural identification and configurational assignment of natural products are challenging tasks.Comprehensive spectral data analysis is crucial for the confirmation of absolute configurations.Ignoring critical parameters will lead to false structure,which may confuse the total synthesis and drug development.Herein,the configurations of seven heterogeneous Pallavicinia diterpenoids(PDs) isolated from Pallavicinia liverworts are revised using a combination of single-crystal X-ray diffraction and electronic circular dichroism(ECD) calculations.Meanwhile,identification of five unprecedented PD heterodimers PD-dimers A-E(18-22) along with eleven previously undescribed PDs(5-9,13-17,23) obtained by the reinvestigation of the Chinese liverwort Pallavicinia subciliata have resulted in corrections and support the revised conclusions.
基金Projects(E2024203001,E2024203049,E2024203071)supported by the Hebei Natural Science Foundation of ChinaProject(236Z1805G)supported by the S&T Program of Hebei,China。
文摘Laser welding is a highly promising joining method for Al alloys.However,certain limitations such as elevated thermal input and keyhole instability are associated with its application in medium-thickness aluminium alloy plates.To address these issues,circular oscillating laser welding combined with post-weld heat treatment was employed to improve the formation quality and mechanical properties of the welds.The effects of the frequency of circular oscillating laser on the forming quality,microstructure,and properties of the welds were analyzed.At an oscillation frequency of 200 Hz,the grain size in the weld zone was reduced compared to single laser welding,and the maximum tensile strength of the weld was observed to reach(264.96±1.33)MPa,representing approximately 61.19%of the base metal.Following the post-weld heat treatment of"solid solution and artificial aging",the grain boundary segregation was diminished.Nanoscale precipitated phases are present in the weld zone.Furthermore,the tensile strength was augmented to(386.35±5.65)MPa,representing approximately 89.23%of the strength of the base metal.The results of this study can provide a theoretical basis and technological reference for the circular oscillating laser welding of medium-thickness 2219-T 6 aluminium alloy plates.
基金supported by the National Natural Science Foundation of China,Nos.82301486(to SL)and 82071325(to FY)Medjaden Academy&Research Foundation for Young Scientists,No.MJR202310040(to SL)+2 种基金Nanjing Medical University Science and Technique Development,No.NMUB20220060(to SL)Medical Scientific Research Project of Jiangsu Commission of Health,No.ZDA2020019(to JZ)Health China Buchang Zhiyuan Public Welfare Project for Heart and Brain Health,No.HIGHER202102(to QD).
文摘Acute ischemic stroke is a clinical emergency and a condition with high morbidity,mortality,and disability.Accurate predictive,diagnostic,and prognostic biomarkers and effective therapeutic targets for acute ischemic stroke remain undetermined.With innovations in high-throughput gene sequencing analysis,many aberrantly expressed non-coding RNAs(ncRNAs)in the brain and peripheral blood after acute ischemic stroke have been found in clinical samples and experimental models.Differentially expressed ncRNAs in the post-stroke brain were demonstrated to play vital roles in pathological processes,leading to neuroprotection or deterioration,thus ncRNAs can serve as therapeutic targets in acute ischemic stroke.Moreover,distinctly expressed ncRNAs in the peripheral blood can be used as biomarkers for acute ischemic stroke prediction,diagnosis,and prognosis.In particular,ncRNAs in peripheral immune cells were recently shown to be involved in the peripheral and brain immune response after acute ischemic stroke.In this review,we consolidate the latest progress of research into the roles of ncRNAs(microRNAs,long ncRNAs,and circular RNAs)in the pathological processes of acute ischemic stroke–induced brain damage,as well as the potential of these ncRNAs to act as biomarkers for acute ischemic stroke prediction,diagnosis,and prognosis.Findings from this review will provide novel ideas for the clinical application of ncRNAs in acute ischemic stroke.
基金National Natural Science Foundation of China(Nos.52174269,52374293)Science and Technology Innovation Program of Hunan Province,China(Nos.2024CK1009,2022RC1123)。
文摘A tunable oxidization and reduction strategy was proposed to directly regenerate spent LiFePO_(4)/C cathode materials by oxidizing excessive carbon powders with the addition of FePO_(4).Experimental results indicate that spent LiFePO_(4)/C cathode materials with good performance can be regenerated by roasting at 650℃ for 11 h with the addition ofLi_(2)CO_(3),FePO_(4),V_(2)O_(5),and glucose.V_(2)O_(5) is added to improve the cycle performance of regenerated cathode materials.Glucose is used to revitalize the carbon layers on the surface of spent LiFePO_(4)/C particles for improving their conductivity.The regenerated V-doped LiFePO_(4)/C shows an excellent electrochemical performance with the discharge specific capacity of 161.36 mA·h/g at 0.2C,under which the capacity retention is 97.85%after 100 cycles.
文摘The rapid growth of the global population,coupled with increasing pollution levels,highlights the urgent need for sustainable and eco-friendly construction materials,such as unfired clay bricks.However,their widespread adoption remains limited due to certain performance drawbacks,particularly in thermal insulation,a critical factor in addressing climate change challenges.In this study,a plant-based waste-derived biopolymer was incorporated into unfired clay bricks to enhance their physicochemical and thermomechanical properties.The biopolymer was added at six different weight fractions(0%,1%,3%,7%,15%,and 20%)to systematically evaluate its impact on bulk density,porosity,capillary water absorption,thermal conductivity,specific heat capacity,and compressive strength.The results revealed a gradual decrease in porosity as the biopolymer content increased,leading to a 41%improvement in thermal conductivity at 20 wt%.However,the optimal balance between thermal efficiency and compressive strength was achieved at 7 wt%biopolymer;this result has been verified through a combination of experimental methods and modeling.Additionally,TRNSYS simulations confirmed the enhanced thermal performance,demonstrating a 9.74%increase in time lag and a 16%reduction in decrement factor,both of which contribute to optimizing building energy efficiency.Overall,this approach not only helps reduce environmental pollution but also enhances insulation capacity while lowering heating and cooling demands,thereby improving overall building performance.Biopolymer-reinforced unfired clay bricks thus represent a promising solution for advancing a low-carbon and sustainable construction industry,aligning with the United Nations Sustainable Development Goals(SDGs)for climate change mitigation and responsible resource management.
基金financially supported by the National Natural Science Foundation of China(No.52204310)the Guizhou Provincial Key Laboratory of Coal Clean Utilization(No.[2020]2001)+5 种基金the China Postdoctoral Science Foundation(Nos.2020TQ0059 and 2020M570967)the Natural Science Foundation of Liaoning Province(No.2021–MS–083)the Fundamental Research Funds for the Central Universities,China(No.N2125010)the Open Project Program of Key Laboratory of Metallurgical Emission Reduction&Resources Recycling(Anhui University of Technology),Ministry of Education(No.JKF22–02)the Foundation of Liupanshui Normal University(No.LPSSYZDZK202205)the Key Laboratory for Anisotropy and Texture of Materials,Ministry of Education,China。
文摘With the continuous increase in the disposal volume of spent lithium-ion batteries(LIBs),properly recycling spent LIBs has become essential for the advancement of the circular economy.This study presents a systematic analysis of the chlorination roasting kinetics and proposes a new two-step chlorination roasting process that integrates thermodynamics for the recycling of LIB cathode materials.The activation energy for the chloride reaction was 88.41 kJ/mol according to thermogravimetric analysis–derivative thermogravimetry data obtained by using model-free,model-fitting,and Z(α)function(αis conversion rate).Results indicated that the reaction was dominated by the first-order(F1)model when the conversion rate was less than or equal to 0.5 and shifted to the second-order(F2)model when the conversion rate exceeded 0.5.Optimal conditions were determined by thoroughly investigating the effects of roasting temperature,roasting time,and the mass ratio of NH_(4)Cl to LiCoO_(2).Under the optimal conditions,namely 400℃,20 min,and NH_(4)Cl/LiCoO_(2)mass ratio of 3:1,the leaching efficiency of Li and Co reached 99.43% and 99.05%,respectively.Analysis of the roasted products revealed that valuable metals in LiCoO_(2)transformed into CoCl_(2) and LiCl.Furthermore,the reaction mechanism was elucidated,providing insights for the establishment of a novel low-temperature chlorination roasting technology based on a crystal structure perspective.This technology can guide the development of LIB recycling processes with low energy consumption,low secondary pollution,high recovery efficiency,and high added value.
文摘Multiple functional metasurfaces with high information capacity have attracted considerable attention from researchers.This study proposes a 2-bit tunable spin-decoupled coded metasurface designed for the terahertz band,which utilizes the tunable properties of Dirac semimetals(DSM)to create a novel multilayer structure.By incorporating both geometric and propagating phases into the metasurface design,we can effectively control the electromagnetic wave.When the Fermi level(EF)of the DSM is set at 6 meV,the electromagnetic wave is manipulated by the gold patch embedded in the DSM film,operating at a frequency of 1.3 THz.When the EF of the DSM is set at 80 meV,the electromagnetic wave is manipulated by the DSM patch,operating at a frequency of 1.4 THz.Both modes enable independent control of beam splitting under left-rotating circularly polarized(LCP)and rightrotating circularly polarized(RCP)wave excitation,resulting in the generation of vortex beams with distinct orbital angular momentum(OAM)modes.The findings of this study hold significant potential for enhancing information capacity and polarization multiplexing techniques in wireless communications.
文摘The development of sustainable sludge management systems requires looking at them with a new vision in which the concepts of SD(Sustainable Development)must integrate those of CE(Circular Economy),both concepts subject to the principles of TD(Thermodynamics),thus allowing the adoption of actions that are all the more effective the more complete the evaluation of the social dimension has been.This involves a new“Way of thinking”which sees the sludge system as the“Locomotive”of the entire wastewater/sludge treatment train and is developed through“Ways of acting”which includes both“Technical”actions to maximize recoveries of useful materials and/or or energy,and“Socio/Institutional”actions to overcome barriers linked to local cultures and traditions,also considering that the specific local context heavily influences the choices capable of satisfying the concepts of CE.It follows the need of issuing realistic and applicable regulations and overcoming social barriers,such as lack of infrastructure and/or qualified personnel,to achieve an effective integration of the concepts of CE with the more general ones of sustainability.
文摘LEADSFON(XIAMEN)TEXTILE TECH CO.,LTD.is a manufacturer of knitting circular machines.Since 2002,the company has served as an ODM and supporting partner for the Italian brand"PILOTELLI".In 2014,LEADSFON officially acquired PILOTELLI,integrating advanced Italian technology into its core operations.
基金supported by grants from the municipal Natural Science Foundation of Shanghai Scientific Committee of China(22ZR1451000 to TL)the peak supporting clinical discipline of Shanghai Health Bureau(2023ZDFC0104 to TL)+3 种基金the key clinical discipline of Shanghai Pudong Health Bureau(PWZxk2022-17 to TL)the clinical peak discipline of Shanghai Pudong Heath Bureau(PWYgf2021-03)the top-notch innovative medical talents of Shanghai Pudong Health Bureau(2025PDWSYCBJ-03 to TL)the leading medical talent project of Shanghai Pudong Heath Bureau(PWR12020-07 to LS)。
文摘BACKGROUND:Circulating biomarkers for sepsis are lacking,and research on circular RNAs(circR NAs)as potential biomarkers of pneumonia-induced sepsis is limited.This study aims to investigate the diagnostic and prognostic potential of circRNAs in patients with pneumonia-induced sepsis.METHODS:This prospective cohort study included 40 healthy individuals,60 patients with pneumonia,and 80 patients with pneumonia-induced sepsis.CircRNAs identified through RNA-sequencing were validated using quantitative real-time polymerase chain reaction(qRT-PCR).Spearman correlation analysis was used to evaluate the associations between circRNAs,inflammatory markers,Sequential Organ Failure Assessment(SOFA)scores,and Acute Physiology and Chronic Health EvaluationⅡ(APACHEⅡ)scores.Receiver operating characteristic(ROC)curves analysis were used to assess the diagnostic performance of circRNAs,while ROC curves and Kaplan-Meier survival analysis were used to evaluate their prognostic value of 28-day mortality.RESULTS:qRT-PCR confirmed the significant upregulation of Circ-CTD-2281E23.2 and downregulation of Circ-0075723 and Circ-0008679 in sepsis patients.Spearman correlation analysis showed that Circ-CTD-2281E23.2 was positively correlated with inflammatory markers and severity scores,whereas Circ-0075723 and Circ-0008679 were negatively correlated with these parameters.The area under the curve(AUC)values for Circ-CTD-2281E23.2,Circ-0075723,and Circ-0008679 in diagnosing pneumonia-induced sepsis were 0.728,0.706,and 0.793,respectively.The combination of these circRNAs(AUC=0.846)and the combination with other clinical indicators(AUC=0.990)demostrated enhanced AUC values.The AUC values for Circ-CTD-2281E23.2 and Circ-0075723 in predicting 28-day mortality were 0.664 and 0.765,respectively.CONCLUSION:This study suggest the additional diagnostic and prognostic value of circRNAs in pneumonia-induced sepsis.Circ-CTD-2281E23.2,Circ-0075723,and Circ-0008679 exhibit diagnostic potential,with Circ-CTD-2281E23.2 and Circ-0075723 showing positive prognostic value for 28-day mortality in sepsis patients.
基金the financial support given by the Ministry of Higher Education Malaysia(MOHE)under the Higher Institution Centre of Excellence(HICOE2.0/5210004)at the Institute of Tropical Forestry and Forest Products.
文摘In this comprehensive review,the evolution and progress of bioplastics are examined,with an emphasis on their types,production methods,environmental impact,and biodegradability.In light of the increasing global efforts to address environmental degradation,bioplastics have emerged as a highly potential substitute for conventional petroleum-based plastics.This review classifies various categories of bioplastics,encompassing both biodegradable and bio-based variations,and assesses their environmental consequences using life cycle evaluations and biodegradability calculations.This paper analyzes the technological advancements that have enhanced the mechanical and thermal characteristics of bioplastics,hence increasing their feasibility for extensive commercial applications in diverse sectors.This review critically examines the possible uses of bioplastics in important industries including packaging,aerospace,and healthcare,emphasizing both achievements and current obstacles.In addition,the assessment addresses the economic and technical obstacles to expanding bioplastic manufacturing,namely concerns about cost,material efficiency,and waste disposal.Moreover,the article forecasts the future potential of bioplastics in furthering a sustainable circular economy and suggests methods to address existing constraints,such as improvements in recycling technology and the establishment of more economically efficient manufacturing methods.The findings are intended to educate policymakers,industry stakeholders,and researchers on the crucial contribution of bioplastics in attaining sustainability objectives and promoting innovation in the field of material science.
基金supported by Beijing Natural Science Foundation of China(Grant No.2244099)the China Postdoctoral Science Foundation(Grant No.2023T0025)the National Natural Science Foundation of China(Grant No.52074020).
文摘Layered rock formations are frequently encountered during the excavation of underground structures. The stability of such structures is influenced not only by the stress concentration caused by the cavities in the strata but also by the anisotropy of the layered rock mass. The interaction between them can lead to critical structural failure, such as rupture, collapse, or significant deformation within the adjacent rock mass, thereby jeopardizing operational safety. However, the coupling law and mechanism between the stress concentration resulting from the cavities and the anisotropy of a layered rock mass remain unclear. In this study, a uniaxial compression test was performed on shale specimens containing a circular hole to investigate the effects of layer inclination and circular holes on the mechanical properties, elastic energy storage, and failure behaviors of these specimens. The failure mechanism of the rock surrounding the hole was analyzed on the basis of the single plane of weakness theory and the Kirsch solution. The test results indicated pronounced anisotropy in the compressive strength, elastic modulus, and elastic strain energy of the specimens, with distinct “V”, “M” and “U”-shaped patterns correlated with varying layer inclination angles. In addition, the combined effect of stress concentration and layer inclination resulted in different failure types, which were classified into four groups according to their failure behavior. Theoretical analysis revealed that failure around circular holes in layered rock is affected by a range of variables, such as layer inclination, layer strength, lateral pressure coefficient, azimuth, and loading stress.
基金Supported by Zhejiang Provincial Natural Science Foundation of China,No.LQ23H020004The Medical and Health Research Project of Zhejiang province,No.2024KY983Basic Medical Health Technology Project of Wenzhou Science and Technology Bureau,No.Y20210818 and No.Y20210140.
文摘BACKGROUND Myocardial ischemia/reperfusion(I/R)injury,which is associated with high morbidity and mortality,is a main cause of unexpected myocardial injury after acute myocardial infarction.However,the underlying mechanism remains unclear.Circular RNAs(circRNAs),which are formed from protein-coding genes,can sequester microRNAs or proteins,modulate transcription and interfere with splicing.Authoritative studies suggest that circRNAs may play an important role in myocardial I/R injury.AIM To explore the role and mechanism of circRNAs in myocardial I/R injury.METHODS We constructed a myocardial I/R injury model using ligation of the left anterior descending coronary artery,and evaluated the success of the validated model using triphenyltetrazolium chloride and hematoxylin-eosin staining.Then,left ventricular samples from different groups were selected for mRNA-sequence,and differential gene screening was performed on the obtained results.The differentially obtained mRNAs were divided into up-regulated and down-regulated according to their expression levels,and Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)functional enrichment analysis were performed,respectively.Then,the obtained circRNA and microRNA(miRNA)were paired for analysis,and the binding sites of miRNA and mRNA were virtual screened.Finally,the obtained circRNA,miRNA and mRNA were constructed by ceRNA mutual most useful network.RESULTS We used an RNA sequencing array to investigate the expression signatures of circRNAs in myocardial I/R injury using three samples from the I/R group and three samples from the sham group.A total of 142 upregulated and 121 downregulated circRNAs were found to be differentially expressed(fold change≥2,P<0.05).GO and KEGG functional analyses of these circRNAs were performed.GO analysis revealed that these circRNAs were involved mainly in cellular and intracellular processes.KEGG analysis demonstrated that 6 of the top 20 pathways were correlated with cell apoptosis.Furthermore,a circRNA-miRNA coexpression network and ceRNA network based on these genes were constructed,revealing that mmu-circ-0001452,mmu-circ-0001637,and mmu-circ-0000870 might be key regulators of myocardial I/R injury.CONCLUSION This research provides new insights into the mechanism of myocardial I/R,which mmu-circ-0001452,mmu-circ-0001637,and mmu-circ-0000870 are expected to be new therapeutic targets for myocardial I/R injury.
