Objective:Chrysanthemi Indici Flos(CIF),the inflorescence of Chrysanthemum indicum L.,is widely used in traditional Chinese medicine.Traditionally,the content of active components in Chinese medicinal materials often ...Objective:Chrysanthemi Indici Flos(CIF),the inflorescence of Chrysanthemum indicum L.,is widely used in traditional Chinese medicine.Traditionally,the content of active components in Chinese medicinal materials often changes during processing.However,the variation patterns of main active components in CIF during drying remain largely unknown.This study aims to investigate the contents of multiple chemical components and in vitro bioactivity of CIF with different drying degrees,so as to provide an optimized scheme for its origin processing.Methods:Fresh CIF were dried at 60℃ to obtain samples with different water contents(80%WC,60%WC,40%WC,20%WC and DS).HPLC was used to determine eight chemical components(chlorogenic acid,3,5-O-dicaffeoylquinic acid,galuteolin,linarin,luteolin,naringenin,kaempferol and isorhamnetin).In vitro assays evaluated antioxidant activity(radical scavenging),anti-inflammatory activity(enzyme inhibition)and antibacterial activity(disk diffusion,MIC)against several strains.Results:Among eight chemical components,chlorogenic acid and linarin decreased significantly with drying,luteolin and kaempferol first increased then decreased(peaking at 40%WC),and naringenin was highest in 80%WC.HPLC fingerprint similarity was lowest(0.559)between 80%WC and DS samples.Antioxidant(Total reducing capacity/DPPH/ABTS/OH IC50:80%WC>DS)and anti-inflammatory(XOD/LOX IC50:80%WC>DS)activities strengthened with drying.Extracts inhibited Staphylococcus aureus(MIC 1.560-3.125 mg/m L),Listeria monocytogenes(DS group inhibition zone larger)and Escherichia coli(MIC 3.125 mg/m L),but not Pseudomonas aeruginosa or Salmonella paratyphoid.Conclusion:Drying degree significantly affects CIF's chemical profiles and in vitro bioactivities,with dry samples showing superior antioxidant/anti-inflammatory properties and specific antibacterial effects.These findings offer targeted guidance for optimizing primary processing parameters of CIF.展开更多
Objective:To explore the molecular mechanism of Mimenghua Granules in treating dry eye based on network pharmacology and bioinformatics methods.Methods:Screening and prediction of possible blood-inducing active ingred...Objective:To explore the molecular mechanism of Mimenghua Granules in treating dry eye based on network pharmacology and bioinformatics methods.Methods:Screening and prediction of possible blood-inducing active ingredients and action target of Mimenghua Granules through Traditional Chinese Medicine Systems Pharmacology database and analysis platform;mining dry eye-related diseases through disease gene database,gene target;use the functional protein combined network database STRING to draw the component-target and disease-target PPI networks,and extract the intersection of these two networks;use DAVID database analysis to screen key targets and analyze the mechanism of action.Results:A total of 593 active ingredients related to Mimenghua Granules were retrieved from the Traditional Chinese Medicine Systems Pharmacology database,and 59 blood active ingredients were obtained by screening based on pharmacokinetic parameters,and 680 targets related to these ingredients were retrieved;from disease genes,the database searches for 47 genes directly related to dry eye;3 key genes(ICAM1,IFNG,and IL-6)were obtained after the intersection of the component target and disease target PPI network;these genes are mainly involved in natural killer cell-mediated cytotoxicity,Jak-STAT signaling pathway,and Cytokine-cytokine receptor interaction.Conclusion:The mechanism of Mimenghua Granules in treating dry eye is related to the interaction of cytotoxic pathway,Jak-STAT signal pathway,and cytokines.The key gene targets are ICAM1,IFNG,and IL-6.展开更多
Objective To establish a RP-HPLC fingerprinting analysis method for quality evaluation and control of the four variants of Chrysanthemi Morifoli Flos.Methods RP-HPLC was used to establish the fingerprinting method.Res...Objective To establish a RP-HPLC fingerprinting analysis method for quality evaluation and control of the four variants of Chrysanthemi Morifoli Flos.Methods RP-HPLC was used to establish the fingerprinting method.Results Despite of the similarity in terms of holistic HPLC chromatograms,the four variants of Chrysanthemi Morifoli Flos exhibit characteristic fingerprints and can be readily recognized by similarity clusters.Conclusion A simple and reliable HPLC fingerprinting method has been developed and validated to authenticate the four variants of Chrysanthemi Morifoli Flos,providing a scientific basis for quality control of Chrysanthemi Morifoli Flos.展开更多
Objective:The purpose of this study is to investigate the anti-diabetic effects of linarin,a flavonoid extracted from Chrysanthemi Indici Flos(CIF),and its potential mechanisms.Methods:The effects of linarin on cell v...Objective:The purpose of this study is to investigate the anti-diabetic effects of linarin,a flavonoid extracted from Chrysanthemi Indici Flos(CIF),and its potential mechanisms.Methods:The effects of linarin on cell viability and glucose consumption in Hep G2 cells were measured.Meanwhile,monosodium glutamate(MSG) mouse model was constructed to monitor the changes of insulin tolerance,glucose tolerance,triglyceride and cholesterol.The protein expression levels of pAMPK,p-ACC,PEPCK and p-GS were detected by Western blot.Results:Linarin could increase the relative glucose consumption of Hep G2 cells,improve insulin tolerance and glucose tolerance,and decrease the levels of triglyceride and cholesterol of MSG mice.Simultaneously,the expression levels of p-AMPK and p-ACC in Hep G2 cells and the liver tissue of MSG mice were increased,while the expression levels of PEPCK and p-GS were decreased after treatment with linarin.Conclusion:Insulin resistance could be ameliorated by linarin in type 2 diabetes,and its mechanism may be related to AMPK signaling pathway.展开更多
基金financially supported by the Upgrade Project of Chinese Pharmacopoeia Committee Pharmaceutical Standard"Chrysanthemum indicum"(No.2021Z013)Opening Project of NMPA Key Laboratory for Quality Research and Evaluation of Traditional Chinese Medicine(No.AHYJ-KFKT-202101)+1 种基金the earmarked fund for Jiangsu Agricultural Industry Technology System(No.JATS[2022]461)the National Natural Science Foundation of China Youth Fund Project(No.82304684)。
文摘Objective:Chrysanthemi Indici Flos(CIF),the inflorescence of Chrysanthemum indicum L.,is widely used in traditional Chinese medicine.Traditionally,the content of active components in Chinese medicinal materials often changes during processing.However,the variation patterns of main active components in CIF during drying remain largely unknown.This study aims to investigate the contents of multiple chemical components and in vitro bioactivity of CIF with different drying degrees,so as to provide an optimized scheme for its origin processing.Methods:Fresh CIF were dried at 60℃ to obtain samples with different water contents(80%WC,60%WC,40%WC,20%WC and DS).HPLC was used to determine eight chemical components(chlorogenic acid,3,5-O-dicaffeoylquinic acid,galuteolin,linarin,luteolin,naringenin,kaempferol and isorhamnetin).In vitro assays evaluated antioxidant activity(radical scavenging),anti-inflammatory activity(enzyme inhibition)and antibacterial activity(disk diffusion,MIC)against several strains.Results:Among eight chemical components,chlorogenic acid and linarin decreased significantly with drying,luteolin and kaempferol first increased then decreased(peaking at 40%WC),and naringenin was highest in 80%WC.HPLC fingerprint similarity was lowest(0.559)between 80%WC and DS samples.Antioxidant(Total reducing capacity/DPPH/ABTS/OH IC50:80%WC>DS)and anti-inflammatory(XOD/LOX IC50:80%WC>DS)activities strengthened with drying.Extracts inhibited Staphylococcus aureus(MIC 1.560-3.125 mg/m L),Listeria monocytogenes(DS group inhibition zone larger)and Escherichia coli(MIC 3.125 mg/m L),but not Pseudomonas aeruginosa or Salmonella paratyphoid.Conclusion:Drying degree significantly affects CIF's chemical profiles and in vitro bioactivities,with dry samples showing superior antioxidant/anti-inflammatory properties and specific antibacterial effects.These findings offer targeted guidance for optimizing primary processing parameters of CIF.
基金Projects supported by the National Natural Science Foundation of China(30772824,81574031)TCM Key Laboratory Construction Project of Hunan Province for Prevention and Treatment of ENT(2017TP1018)+3 种基金Central financial support for local university construction projects(2018-2019)State Administration of Traditional Chinese Medicine Ophthalmology Key Discipline Construction Project(ZK1801YK015)Key Discipline Construction Project of TCM Five Sense Organs in Hunan ProvinceHunan Graduate Research Innovation Project(CX20200783).
文摘Objective:To explore the molecular mechanism of Mimenghua Granules in treating dry eye based on network pharmacology and bioinformatics methods.Methods:Screening and prediction of possible blood-inducing active ingredients and action target of Mimenghua Granules through Traditional Chinese Medicine Systems Pharmacology database and analysis platform;mining dry eye-related diseases through disease gene database,gene target;use the functional protein combined network database STRING to draw the component-target and disease-target PPI networks,and extract the intersection of these two networks;use DAVID database analysis to screen key targets and analyze the mechanism of action.Results:A total of 593 active ingredients related to Mimenghua Granules were retrieved from the Traditional Chinese Medicine Systems Pharmacology database,and 59 blood active ingredients were obtained by screening based on pharmacokinetic parameters,and 680 targets related to these ingredients were retrieved;from disease genes,the database searches for 47 genes directly related to dry eye;3 key genes(ICAM1,IFNG,and IL-6)were obtained after the intersection of the component target and disease target PPI network;these genes are mainly involved in natural killer cell-mediated cytotoxicity,Jak-STAT signaling pathway,and Cytokine-cytokine receptor interaction.Conclusion:The mechanism of Mimenghua Granules in treating dry eye is related to the interaction of cytotoxic pathway,Jak-STAT signal pathway,and cytokines.The key gene targets are ICAM1,IFNG,and IL-6.
文摘Objective To establish a RP-HPLC fingerprinting analysis method for quality evaluation and control of the four variants of Chrysanthemi Morifoli Flos.Methods RP-HPLC was used to establish the fingerprinting method.Results Despite of the similarity in terms of holistic HPLC chromatograms,the four variants of Chrysanthemi Morifoli Flos exhibit characteristic fingerprints and can be readily recognized by similarity clusters.Conclusion A simple and reliable HPLC fingerprinting method has been developed and validated to authenticate the four variants of Chrysanthemi Morifoli Flos,providing a scientific basis for quality control of Chrysanthemi Morifoli Flos.
基金supported by the National Natural Science Foundation of China (grant numbers 81273392)the Natural Science Foundation of Henan Province (grant numbers 182300410348)
文摘Objective:The purpose of this study is to investigate the anti-diabetic effects of linarin,a flavonoid extracted from Chrysanthemi Indici Flos(CIF),and its potential mechanisms.Methods:The effects of linarin on cell viability and glucose consumption in Hep G2 cells were measured.Meanwhile,monosodium glutamate(MSG) mouse model was constructed to monitor the changes of insulin tolerance,glucose tolerance,triglyceride and cholesterol.The protein expression levels of pAMPK,p-ACC,PEPCK and p-GS were detected by Western blot.Results:Linarin could increase the relative glucose consumption of Hep G2 cells,improve insulin tolerance and glucose tolerance,and decrease the levels of triglyceride and cholesterol of MSG mice.Simultaneously,the expression levels of p-AMPK and p-ACC in Hep G2 cells and the liver tissue of MSG mice were increased,while the expression levels of PEPCK and p-GS were decreased after treatment with linarin.Conclusion:Insulin resistance could be ameliorated by linarin in type 2 diabetes,and its mechanism may be related to AMPK signaling pathway.
