Rapid and accessible detection of E.coli remains a critical challenge in ensuring food and water safety,particularly in resource-limited settings.This study presents the design,fabrication,and evaluation of an analyti...Rapid and accessible detection of E.coli remains a critical challenge in ensuring food and water safety,particularly in resource-limited settings.This study presents the design,fabrication,and evaluation of an analytical device for the enzymatic colorimetric detection of E.coli in milk and water,without requiring sample pretreatment.The device,constructed using a 3D-printed PET-G cartridge housing a cotton pad substrate,employs X-Gal as a chromogenic substrate,APTES as a surface modifier,and SB3-12 as a lysis agent,optimized via an orthogonal experimental design.Performance assessment revealed a linear detection range of 10^(2)-10^(5)CFU/mL,a limit of detection(LOD)of 9.6×10^(1)CFU/mL,and a total assay time of 7 h,with color intensity quantifiable visually or via mobile phone imaging and MATLAB analysis.The analytical device demonstrated high selectivity against non-target bacteria(Salmonella enterica,Enterococcus faecalis,Bacillus subtilis)and chemical contaminants,alongside stability exceeding 90%accuracy over three months under controlled storage.These results underscore the device’s potential as a cost-effective,user-friendly tool for on-site E.coli monitoring,advancing pointof-care diagnostics for public health and food safety applications.Future improvements could target shortening assay duration,increasing precision and sensitivity,and broadening the range of detectable pathogens.展开更多
基金supported by Shiraz University and Shahid Sadoughi University of Medical Sciences.
文摘Rapid and accessible detection of E.coli remains a critical challenge in ensuring food and water safety,particularly in resource-limited settings.This study presents the design,fabrication,and evaluation of an analytical device for the enzymatic colorimetric detection of E.coli in milk and water,without requiring sample pretreatment.The device,constructed using a 3D-printed PET-G cartridge housing a cotton pad substrate,employs X-Gal as a chromogenic substrate,APTES as a surface modifier,and SB3-12 as a lysis agent,optimized via an orthogonal experimental design.Performance assessment revealed a linear detection range of 10^(2)-10^(5)CFU/mL,a limit of detection(LOD)of 9.6×10^(1)CFU/mL,and a total assay time of 7 h,with color intensity quantifiable visually or via mobile phone imaging and MATLAB analysis.The analytical device demonstrated high selectivity against non-target bacteria(Salmonella enterica,Enterococcus faecalis,Bacillus subtilis)and chemical contaminants,alongside stability exceeding 90%accuracy over three months under controlled storage.These results underscore the device’s potential as a cost-effective,user-friendly tool for on-site E.coli monitoring,advancing pointof-care diagnostics for public health and food safety applications.Future improvements could target shortening assay duration,increasing precision and sensitivity,and broadening the range of detectable pathogens.
