As sessile organisms, plants display a high capacity for phenotypic adaptations allowing them to meet the constraints of a changing local environment. This plasticity notably originates from the aptitude of plants to ...As sessile organisms, plants display a high capacity for phenotypic adaptations allowing them to meet the constraints of a changing local environment. This plasticity notably originates from the aptitude of plants to adjust their development and physiology throughout their lifetime to reach an optimal trade-off between growth and fitness. As in all eukaryotes, dynamic control of plant gene expression in different cell types is dependent on multiple regulatory layers that often converge onto chromatin-based mechanisms. Dynamic control of chromatin composition and organization has a strong impact on the cellular transcriptional program, notably contributing to selective modulation of gene expression in a reversible manner. Chromatin state changes generally involve remodeling of nu cleosome positio ning, incorporatio n of nu cleosomal and lin ker histone variants, and DNA and histone modifications recognized by cognate readers. Together, these processes modulate the access and activity of trans-acting factors such as transcription factors and RNA polymerases. Temporary shifts between transcriptionally permissive and repressive chromatin states rely on various mechanisms, among which is the rapid reversion of a “responsive” chromatin status through active erasure of newly established features.展开更多
染色质转座酶可及性测序(assay for transposase-accessible chromatin with high-throughput sequencing,ATAC-seq)是利用Tn5转座酶研究染色质可及性的高通量测序技术。ATAC-seq可以在全基因组范围内绘制染色质可及性图谱,揭示转录因...染色质转座酶可及性测序(assay for transposase-accessible chromatin with high-throughput sequencing,ATAC-seq)是利用Tn5转座酶研究染色质可及性的高通量测序技术。ATAC-seq可以在全基因组范围内绘制染色质可及性图谱,揭示转录因子结合位点以及核小体的位置。在医学领域,ATAC-seq技术是研究重大疾病发病机制、药物作用机制、新药研发和生物标志物功能等的新一代有力工具。本文对ATAC-seq技术的优势及其在复杂疾病研究中的应用和前景进行了综述,以期为人类复杂疾病基因表达调控机制等相关研究的开展提供借鉴与参考。展开更多
文摘As sessile organisms, plants display a high capacity for phenotypic adaptations allowing them to meet the constraints of a changing local environment. This plasticity notably originates from the aptitude of plants to adjust their development and physiology throughout their lifetime to reach an optimal trade-off between growth and fitness. As in all eukaryotes, dynamic control of plant gene expression in different cell types is dependent on multiple regulatory layers that often converge onto chromatin-based mechanisms. Dynamic control of chromatin composition and organization has a strong impact on the cellular transcriptional program, notably contributing to selective modulation of gene expression in a reversible manner. Chromatin state changes generally involve remodeling of nu cleosome positio ning, incorporatio n of nu cleosomal and lin ker histone variants, and DNA and histone modifications recognized by cognate readers. Together, these processes modulate the access and activity of trans-acting factors such as transcription factors and RNA polymerases. Temporary shifts between transcriptionally permissive and repressive chromatin states rely on various mechanisms, among which is the rapid reversion of a “responsive” chromatin status through active erasure of newly established features.
文摘染色质转座酶可及性测序(assay for transposase-accessible chromatin with high-throughput sequencing,ATAC-seq)是利用Tn5转座酶研究染色质可及性的高通量测序技术。ATAC-seq可以在全基因组范围内绘制染色质可及性图谱,揭示转录因子结合位点以及核小体的位置。在医学领域,ATAC-seq技术是研究重大疾病发病机制、药物作用机制、新药研发和生物标志物功能等的新一代有力工具。本文对ATAC-seq技术的优势及其在复杂疾病研究中的应用和前景进行了综述,以期为人类复杂疾病基因表达调控机制等相关研究的开展提供借鉴与参考。
