The esterifications of 9-（hydroxyimino）-4-methyl-8,9-dihydrofuro[2,3-h]chromen-2-one （4） with acid chlorides afforded normal oxime-esters 3a-e in 35-78% yields in presence of excessive 4-dimethylaminopyridine as t...The esterifications of 9-（hydroxyimino）-4-methyl-8,9-dihydrofuro[2,3-h]chromen-2-one （4） with acid chlorides afforded normal oxime-esters 3a-e in 35-78% yields in presence of excessive 4-dimethylaminopyridine as the acid scavenger, whereas the reactions gave unexpected 8-substituted products N-（8-chloro-4-methyl-2-oxo-2H-furo-[2,3-h]chromen-9-yl）amides （5a-c） and 4-methyl-2,9-dioxo-8,9-dihydro-2H-furo[2,3-h]chromen-8-ylcarboxyloates （6d-e） by using excessive acid chlorides. The structures of 10 new compounds were determined by 1H NMR, 13C NMR, MS and HRMS, and the possible mechanism for the formation of unexpected products 5a--c and 6d-e was also proposed.展开更多
Chromosome microarray analysis(CMA) is a cost-effective molecular cytogenetic technique that has been used as a first-line diagnostic test in neurodevelopmental disorders in the USA since 2011. The impact of CMA resul...Chromosome microarray analysis(CMA) is a cost-effective molecular cytogenetic technique that has been used as a first-line diagnostic test in neurodevelopmental disorders in the USA since 2011. The impact of CMA results on clinical practice in China is not yet well studied, so we aimed to better evaluate this phenomenon.We analyzed the CMA results from 434 patients in our clinic, and characterized their molecular diagnoses, clinical features, and follow-up clinical actions based on these results. The overall diagnostic yield for our patients was 13.6%(59 out of 434). This gave a detection rate of 14.7%for developmental delay/intellectual disability(DD/ID,38/259) and 12% for autism spectrum disorders(ASDs,21/175). Thirty-three recurrent(n≥2) variants were found, distributed at six chromosomal loci involving known chromosome syndromes(such as DiGeorge, Williams Beuren, and Angelman/Prader-Willi syndromes).The spectrum of positive copy number variants in our study was comparable to that reported in Caucasian populations, but with specific characteristics. Parental origin tests indicated an effect involving a significant maternal transmission bias to sons. The majority of patients with positive results(94.9%) had benefits, allowing earlier diagnosis(36/59), prioritized full clinical management(28/59), medication changes(7/59), a changed prognosis(30/59), and prenatal genetic counseling(15/59). Our results provide information on de novo mutations in Chinese children with DD/ID and/or ASDs. Our data showed that microarray testing provides immediate clinical utility for patients. It is expected that the personalized medical care of children with developmental disabilities will lead to improved outcomes in long-term developmental potential.We advocate using the diagnostic yield of clinically actionable results to evaluate CMA as it provides information of both clinical validity and clinical utility.展开更多
ABSTRACT A quantitative method for the assay of free cholesterol has been described in this paper. The experimental conditions for the determination of cholesterol in serum by Thin-layer chromatography were disscused....ABSTRACT A quantitative method for the assay of free cholesterol has been described in this paper. The experimental conditions for the determination of cholesterol in serum by Thin-layer chromatography were disscused. The solvent System was petroleum ether-ethyl acetate-glacial acetic acid (8o:20:1) and the spra-ying reagent was a solution of sulphuric acid and vanillin. Under the selected con-ditions, the peak area was linearly related to the cholesterol amount for the range between 80～700 ng per spot. The intraplate and interplate coefficients were 2.4% and 7.4% respectively. The recovery of cholesterol was 101.6%. The method presented was simple, rapid and accurate. The results of experi-mental investigation and clinical application were satisfactory.展开更多
The optimum conditions for the extraction-chromatography by using 2-ethylhexyl-2-ethylhexyl- phosphonate resin to separate scandium(Ⅲ)from large amounts of other metal ions were reported.A me- thod for the separation...The optimum conditions for the extraction-chromatography by using 2-ethylhexyl-2-ethylhexyl- phosphonate resin to separate scandium(Ⅲ)from large amounts of other metal ions were reported.A me- thod for the separation and determination of microamount of scandium has been developed.This method gives higher accuracy and reproducibility than solvent extraction with PMBP,especially,for the samples containing high content of titanium.Many shortcomings of other methods ever reported can be overcome by the present method.展开更多
The chromosomes 1, 3, 5, 6, 7,10 and 12 of rice field eel (Monopterus albus Zuiew) have been microdissected successfully from meiosis I diakinesis spreads by using glass microneedlc under an inverted microscope. And t...The chromosomes 1, 3, 5, 6, 7,10 and 12 of rice field eel (Monopterus albus Zuiew) have been microdissected successfully from meiosis I diakinesis spreads by using glass microneedlc under an inverted microscope. And the DOP-PCR products of the single chromosome dotted on the nylon membrane as 'specific chromosomal DNA pool', have been hybridized with 6 probes to map these genes. The mapping results show that Zfa has been mapped to chromosome 1, rDNA to chromosomes 3 and 7, both Gh and Pdegγto chromosome 10, Hsl to chromosome 5 and Hox genes have been detected on chromosomes 1, 3, 6 and 10 meantime. It has initiatively been suggested that chromosome 10 of rice field eel might possess the commom conserved synteny to that on chromosome 17 of human, chromosome 11 of mouse, chromosome 12 of pig and chromosome 19 of bovine. And so chromosome 3 of rice field eel might also contain the commom conserved synteny to that on chromosome 2 of zebraf-ish. Our study is an attempt to establish a new and feasible展开更多
基金grants from the National Natural Science Foundation of China (No.20272010 and 20672022)
文摘The esterifications of 9-（hydroxyimino）-4-methyl-8,9-dihydrofuro[2,3-h]chromen-2-one （4） with acid chlorides afforded normal oxime-esters 3a-e in 35-78% yields in presence of excessive 4-dimethylaminopyridine as the acid scavenger, whereas the reactions gave unexpected 8-substituted products N-（8-chloro-4-methyl-2-oxo-2H-furo-[2,3-h]chromen-9-yl）amides （5a-c） and 4-methyl-2,9-dioxo-8,9-dihydro-2H-furo[2,3-h]chromen-8-ylcarboxyloates （6d-e） by using excessive acid chlorides. The structures of 10 new compounds were determined by 1H NMR, 13C NMR, MS and HRMS, and the possible mechanism for the formation of unexpected products 5a--c and 6d-e was also proposed.
基金supported by grants from the National Natural Science Foundation of China (81761128035 and 81781220701)the Shanghai Municipal Science and Technology Committee (17XD1403200 and 18dz2313505)+2 种基金the Research Physician Project of Shanghai Municipal Education Commission (20152234)the Shanghai Municipal Health and Family Planning Commission (GDEK201709, 2017ZZ02026, and 2017EKHWYX02)the Scientific Program of Shanghai Shenkang Hospital Development Center (16CR2025B) of China
文摘Chromosome microarray analysis(CMA) is a cost-effective molecular cytogenetic technique that has been used as a first-line diagnostic test in neurodevelopmental disorders in the USA since 2011. The impact of CMA results on clinical practice in China is not yet well studied, so we aimed to better evaluate this phenomenon.We analyzed the CMA results from 434 patients in our clinic, and characterized their molecular diagnoses, clinical features, and follow-up clinical actions based on these results. The overall diagnostic yield for our patients was 13.6%(59 out of 434). This gave a detection rate of 14.7%for developmental delay/intellectual disability(DD/ID,38/259) and 12% for autism spectrum disorders(ASDs,21/175). Thirty-three recurrent(n≥2) variants were found, distributed at six chromosomal loci involving known chromosome syndromes(such as DiGeorge, Williams Beuren, and Angelman/Prader-Willi syndromes).The spectrum of positive copy number variants in our study was comparable to that reported in Caucasian populations, but with specific characteristics. Parental origin tests indicated an effect involving a significant maternal transmission bias to sons. The majority of patients with positive results(94.9%) had benefits, allowing earlier diagnosis(36/59), prioritized full clinical management(28/59), medication changes(7/59), a changed prognosis(30/59), and prenatal genetic counseling(15/59). Our results provide information on de novo mutations in Chinese children with DD/ID and/or ASDs. Our data showed that microarray testing provides immediate clinical utility for patients. It is expected that the personalized medical care of children with developmental disabilities will lead to improved outcomes in long-term developmental potential.We advocate using the diagnostic yield of clinically actionable results to evaluate CMA as it provides information of both clinical validity and clinical utility.
文摘ABSTRACT A quantitative method for the assay of free cholesterol has been described in this paper. The experimental conditions for the determination of cholesterol in serum by Thin-layer chromatography were disscused. The solvent System was petroleum ether-ethyl acetate-glacial acetic acid (8o:20:1) and the spra-ying reagent was a solution of sulphuric acid and vanillin. Under the selected con-ditions, the peak area was linearly related to the cholesterol amount for the range between 80～700 ng per spot. The intraplate and interplate coefficients were 2.4% and 7.4% respectively. The recovery of cholesterol was 101.6%. The method presented was simple, rapid and accurate. The results of experi-mental investigation and clinical application were satisfactory.
文摘The optimum conditions for the extraction-chromatography by using 2-ethylhexyl-2-ethylhexyl- phosphonate resin to separate scandium(Ⅲ)from large amounts of other metal ions were reported.A me- thod for the separation and determination of microamount of scandium has been developed.This method gives higher accuracy and reproducibility than solvent extraction with PMBP,especially,for the samples containing high content of titanium.Many shortcomings of other methods ever reported can be overcome by the present method.
基金This work was supported by the National Natural Science Foundation of China (Grant No. 39970406)the Research Fund for the Doctoral Program of Higher Education of China (Grant No. 98048628).
文摘The chromosomes 1, 3, 5, 6, 7,10 and 12 of rice field eel (Monopterus albus Zuiew) have been microdissected successfully from meiosis I diakinesis spreads by using glass microneedlc under an inverted microscope. And the DOP-PCR products of the single chromosome dotted on the nylon membrane as 'specific chromosomal DNA pool', have been hybridized with 6 probes to map these genes. The mapping results show that Zfa has been mapped to chromosome 1, rDNA to chromosomes 3 and 7, both Gh and Pdegγto chromosome 10, Hsl to chromosome 5 and Hox genes have been detected on chromosomes 1, 3, 6 and 10 meantime. It has initiatively been suggested that chromosome 10 of rice field eel might possess the commom conserved synteny to that on chromosome 17 of human, chromosome 11 of mouse, chromosome 12 of pig and chromosome 19 of bovine. And so chromosome 3 of rice field eel might also contain the commom conserved synteny to that on chromosome 2 of zebraf-ish. Our study is an attempt to establish a new and feasible
