Obesity has become a significant global public health issue.Previous studies have found that the Chenpi has the anti-obesity activity.However,the anti-obesity phytochemicals and their mechanisms are still unclear.This...Obesity has become a significant global public health issue.Previous studies have found that the Chenpi has the anti-obesity activity.However,the anti-obesity phytochemicals and their mechanisms are still unclear.This study investigated the anti-obesity phytochemicals and molecular mechanisms involved in treating obesity by Chenpi through network pharmacology and molecular docking.A total of 17 bioactive phytochemicals from Chenpi and its 475 related anti-obesity targets have been identified.The KEGG pathway analysis showed that the PI3K/Akt signaling pathway,MAPK signaling pathway,AMPK signaling pathway,and nuclear factor kappa B signaling pathway are the main signaling pathways involved in the anti-obesity effect of Chenpi.According to molecular docking analysis,the phytochemicals of Chenpi can bind to central anti-obesity targets.Based on the ADMET analysis and network pharmacology results,tangeretin exhibited the lowest predicted toxicity and potential for anti-obesity effects.In the in vitro lipid accumulation model,tangeretin effectively suppressed the free fatty acid-induced lipid in Hep G2 cells by upregulating the PI3K/Akt/GSK3βsignaling pathway based on the result of q-PCR and Western blotting.The outcomes of this research give insights for future research on the anti-obesity phytochemicals and molecular mechanisms derived from Chenpi,also providing the theoretical basis for developing anti-obesity functional foods based on Chenpi.展开更多
To ensure the safety of the commercially available chenpi,a convenient and fast analytical method was developed for the determination of 133 pesticide residues in chenpi using gas chromatography-tandem mass spectromet...To ensure the safety of the commercially available chenpi,a convenient and fast analytical method was developed for the determination of 133 pesticide residues in chenpi using gas chromatography-tandem mass spectrometry(GC-MS/MS).In this study,different extraction solvents,redissolution solvents and adsorbents were tested according to the recovery and purification effect to obtain a modified QuEChERS method.The samples were extracted with acetonitrile.During the clean-up step,octadecyl-modified silica(C18)and graphitized carbon black(GCB)were selected,and aminopropyl(NH2)was used instead of primary secondary amine(PSA)because of its weaker ion exchange capacity which had little effect on the recovery of ditalimfos.Samples were quantified by matrix-matched calibration with internal standards.All pesticides showed good linearity in the respective range,both with values of r2>0.99.The average recoveries of the pesticides spiked samples ranged from 70.0% to 112.2% with the RSDs of 0.2%–14.4%.The modified QuEChERS method was validated and applied to twenty real samples.Five pesticides were found in eight batches,but no pesticide exceeded the maximum residue limits(MRL,MRL reference to European commission).展开更多
Biomass-derived carbon dots(C-dots)are considered a very important carbon material in metal ion detection of their small environmental impact,simple preparation process,and relatively low cost.A green approach for syn...Biomass-derived carbon dots(C-dots)are considered a very important carbon material in metal ion detection of their small environmental impact,simple preparation process,and relatively low cost.A green approach for synthesizing biomass-derived C-dots from Chenpi using a hydrothermal method without further processing is proposed in the present study.The as-synthesized C-dots show excellent fluorescence properties,superior resistance to UV irradiation photobleaching,and high photostability in salt-containing solutions.The C-dots were used in the form of label-free fluorescent probes for sensitively detecting Hg^(2+)selectively.The outcome relationship behaved linearly and was established based on a given range between 10–300 nM concentration,with a detection limit of 7.0 nM.This green strategy obtains a high C-dot quantum yield of 10.8%and satisfactory results in detecting Hg^(2+)in actual water samples.展开更多
Objective:By the method of network pharmacology to study the main active compounds,main target genes,critical path and mechanism of the two classical Chinese herbs Chenpi-Banxia in the treatment of Coronary Microvascu...Objective:By the method of network pharmacology to study the main active compounds,main target genes,critical path and mechanism of the two classical Chinese herbs Chenpi-Banxia in the treatment of Coronary Microvascular Dysfunction(CMD).Methods:Obtaining potential active compounds of Chenpi-Banxia from TCMSP,while the targets for CMD were obtained from DrugBank and OMIM databases.Using UniProt database to query the corresponding gene name.The key target of Chenpi-Banxia in the treatment of Coronary Microvascular Dysfunction can be obtained by using the intersection of VENNY.The PPI network was screened for the major targets by String and Cytoscape3.7.1.The GO enrichment analysis and KEGG Pathway analyses of major targets were performed by using the DAVID database and use Binformatics to draw bubble map.Finally,the ingredient-major arget-key pathway network was constructed by Cytoscape3.7.1.Results:There were 16 compounds such as naringenin,nobiletin,baicalein.beta-sitosterol etc,and 56 predictive target genes such as AKT1、VEGFA、BCL2、BAX、JUN etc,as well as 20 key pathways including inflammation-related pathway(TNF signaling pathway),pathways related to cardiovascular system(PI3K-Akt signaling pathway),Vascular endothelial growth factor signaling pathway(VEGF signaling pathway),Apoptosis related pathways(Apoptosis)and Hypoxia cell stress signaling pathway(HIF-1 signaling pathway)in the Compounds-Target-Pathway network.Conclusion:The study verified the characteristics of multi-components,multi-targets and integral regulation for Chenpi-Banxia with the application of network pharmacology.It predicted that Chenpi-Banxia couldtreat Coronary Microvascular Dysfunction mainly by protecting endothelial cell function of coronary microcirculation,inhibiting cell apoptosis and affecting inflammatory reaction.展开更多
目的:探究不同陈化年限衢陈皮的差异代谢物,为衢陈皮的陈化机制和开发利用提供基础和科学依据。方法:采用超高液相色谱-串联质谱(Ultra Performance Liquid Chromatography Tandem Mass Spectrometry,UPLCMS/MS)广泛靶向代谢组学技术对...目的:探究不同陈化年限衢陈皮的差异代谢物,为衢陈皮的陈化机制和开发利用提供基础和科学依据。方法:采用超高液相色谱-串联质谱(Ultra Performance Liquid Chromatography Tandem Mass Spectrometry,UPLCMS/MS)广泛靶向代谢组学技术对4种陈化年限(Y1、Y3、Y5、Y7)衢陈皮的代谢物进行检测和分析;使用主成分分析(Principal Component Analysis,PCA)和正交偏最小二乘法-判别分析(Orthogonal Projections to Latent Structures-Discriminant Analysis,OPLS-DA)统计分析方法鉴定差异代谢物。结果:共鉴定出2019个代谢物,主要为酚酸类、黄酮类、萜类和生物碱类化合物。根据变量投影重要度(Variable Importance Projection,VIP)值大于1且P-value小于0.05,筛选出显著差异代谢物280个,利用KEGG数据库对显著差异代谢物进行KEGG通路富集分析,发现显著差异代谢物主要涉及单萜类生物合成、色氨酸代谢、黄酮和黄酮醇生物合成、花青素的生物合成及托烷、哌啶和吡啶生物碱的生物合成等23条代谢途径。进一步对显著差异代谢物的嗅、味特性进行分析,发现苯乙醇、5-羟甲基糠醛、间甲苯酚、柚皮素、3-甲基苯甲醛、二氢猕猴桃内酯、香兰素等13种呈味代谢物质含量的显著变化可能是衢陈皮陈化过程中颜色、气味、滋味改变的主要物质基础。结论:广泛靶向代谢组学技术可以高效地对衢陈皮化学成分进行组分鉴别和分析,为衢陈皮陈化机制研究和开发利用奠定了重要的技术基础。展开更多
基金supported by the Guangdong Provincial Key Laboratory IRADS(2022B1212010006,R0400001-22)。
文摘Obesity has become a significant global public health issue.Previous studies have found that the Chenpi has the anti-obesity activity.However,the anti-obesity phytochemicals and their mechanisms are still unclear.This study investigated the anti-obesity phytochemicals and molecular mechanisms involved in treating obesity by Chenpi through network pharmacology and molecular docking.A total of 17 bioactive phytochemicals from Chenpi and its 475 related anti-obesity targets have been identified.The KEGG pathway analysis showed that the PI3K/Akt signaling pathway,MAPK signaling pathway,AMPK signaling pathway,and nuclear factor kappa B signaling pathway are the main signaling pathways involved in the anti-obesity effect of Chenpi.According to molecular docking analysis,the phytochemicals of Chenpi can bind to central anti-obesity targets.Based on the ADMET analysis and network pharmacology results,tangeretin exhibited the lowest predicted toxicity and potential for anti-obesity effects.In the in vitro lipid accumulation model,tangeretin effectively suppressed the free fatty acid-induced lipid in Hep G2 cells by upregulating the PI3K/Akt/GSK3βsignaling pathway based on the result of q-PCR and Western blotting.The outcomes of this research give insights for future research on the anti-obesity phytochemicals and molecular mechanisms derived from Chenpi,also providing the theoretical basis for developing anti-obesity functional foods based on Chenpi.
文摘To ensure the safety of the commercially available chenpi,a convenient and fast analytical method was developed for the determination of 133 pesticide residues in chenpi using gas chromatography-tandem mass spectrometry(GC-MS/MS).In this study,different extraction solvents,redissolution solvents and adsorbents were tested according to the recovery and purification effect to obtain a modified QuEChERS method.The samples were extracted with acetonitrile.During the clean-up step,octadecyl-modified silica(C18)and graphitized carbon black(GCB)were selected,and aminopropyl(NH2)was used instead of primary secondary amine(PSA)because of its weaker ion exchange capacity which had little effect on the recovery of ditalimfos.Samples were quantified by matrix-matched calibration with internal standards.All pesticides showed good linearity in the respective range,both with values of r2>0.99.The average recoveries of the pesticides spiked samples ranged from 70.0% to 112.2% with the RSDs of 0.2%–14.4%.The modified QuEChERS method was validated and applied to twenty real samples.Five pesticides were found in eight batches,but no pesticide exceeded the maximum residue limits(MRL,MRL reference to European commission).
基金financially supported by the Development Program of Hunan Province (No.2020SK2128).
文摘Biomass-derived carbon dots(C-dots)are considered a very important carbon material in metal ion detection of their small environmental impact,simple preparation process,and relatively low cost.A green approach for synthesizing biomass-derived C-dots from Chenpi using a hydrothermal method without further processing is proposed in the present study.The as-synthesized C-dots show excellent fluorescence properties,superior resistance to UV irradiation photobleaching,and high photostability in salt-containing solutions.The C-dots were used in the form of label-free fluorescent probes for sensitively detecting Hg^(2+)selectively.The outcome relationship behaved linearly and was established based on a given range between 10–300 nM concentration,with a detection limit of 7.0 nM.This green strategy obtains a high C-dot quantum yield of 10.8%and satisfactory results in detecting Hg^(2+)in actual water samples.
基金National TCM Clinical Research Base Business Construction Research Project of National Administration of Traditional Chinese Medicine(No.JDZX2015033)Liaoning Provincial Science and Technology Fund Project(No.20180530016)Liaoning Provincial Distinguished Professor Program[No.(2017)3]。
文摘Objective:By the method of network pharmacology to study the main active compounds,main target genes,critical path and mechanism of the two classical Chinese herbs Chenpi-Banxia in the treatment of Coronary Microvascular Dysfunction(CMD).Methods:Obtaining potential active compounds of Chenpi-Banxia from TCMSP,while the targets for CMD were obtained from DrugBank and OMIM databases.Using UniProt database to query the corresponding gene name.The key target of Chenpi-Banxia in the treatment of Coronary Microvascular Dysfunction can be obtained by using the intersection of VENNY.The PPI network was screened for the major targets by String and Cytoscape3.7.1.The GO enrichment analysis and KEGG Pathway analyses of major targets were performed by using the DAVID database and use Binformatics to draw bubble map.Finally,the ingredient-major arget-key pathway network was constructed by Cytoscape3.7.1.Results:There were 16 compounds such as naringenin,nobiletin,baicalein.beta-sitosterol etc,and 56 predictive target genes such as AKT1、VEGFA、BCL2、BAX、JUN etc,as well as 20 key pathways including inflammation-related pathway(TNF signaling pathway),pathways related to cardiovascular system(PI3K-Akt signaling pathway),Vascular endothelial growth factor signaling pathway(VEGF signaling pathway),Apoptosis related pathways(Apoptosis)and Hypoxia cell stress signaling pathway(HIF-1 signaling pathway)in the Compounds-Target-Pathway network.Conclusion:The study verified the characteristics of multi-components,multi-targets and integral regulation for Chenpi-Banxia with the application of network pharmacology.It predicted that Chenpi-Banxia couldtreat Coronary Microvascular Dysfunction mainly by protecting endothelial cell function of coronary microcirculation,inhibiting cell apoptosis and affecting inflammatory reaction.
