Background: The allo-immune response following organ transplantation constitutes one of the main determinants concerning both short- and long- term outcomes in renal graft recipients. Chemokines and their receptors pl...Background: The allo-immune response following organ transplantation constitutes one of the main determinants concerning both short- and long- term outcomes in renal graft recipients. Chemokines and their receptors play a diversified and important role, either homeostatic or inflammatory and direct different immune-competent cell types to the allograft. While deeply studied in the last two decades, controversy persists as a result of chemokines’ pleiotropic actions. We report our analysis of CCR1, CCR3, CCR7, CCL5 and CX3CL1 expression or synthesis by graft-infiltrating cells in human kidney transplants (KTx). At the same time, we tested their robustness in diagnosing acute rejection. Methods: Fine-needle aspiration biopsies (Fnab) were performed either on days 7 or 14 post-transplantation among stable KTx and on the day of acute rejection (AR) diagnosis. Fnab cytopreparations were studied by the enzymatic avidin-biotin complex staining for CCR1, CCR3, CCR7 and CX3CL1. From another subgroup of cases, Fnab samples were cultured for 48 hours and the supernatants were analysed for CCL5 by ELISA. Results: The group of AR cases showed a significantly up-regulated expression of CCR1, CCR3, CCR7 and CX3CL1 and a significantly higher synthesis of CCL5. The positive predictive values were respectively 92%, 97%, 85%, 76% and 78% and negative predictive values were by the same order, 100%, 73%, 100%, 98% and 83%. Conclusions: Our study permits us to advance that CCR1 and CCR3 play a significant and non-redundant role in acute rejection, and it is the first report of CCR3 association with rejection, probably related to CCL5. The presence inside the graft of significant up-regulation for CCR7 surmises that part of antigen presentation may be performed there without being restricted to secondary lymphoid sites. Our results with CX3CL1 confirm other reports.展开更多
Chemokines can be divided into four categories: α, β, γ, and δ. Chemokine α is related to neutrophil chemotaxis. Chemokine β is correlated with adsorption of monocytes,basophils, and eosinophils. Chemokine γ is...Chemokines can be divided into four categories: α, β, γ, and δ. Chemokine α is related to neutrophil chemotaxis. Chemokine β is correlated with adsorption of monocytes,basophils, and eosinophils. Chemokine γ is mainly a lymphocyte chemokine. Function of chemokine δ remains unclear. Chemokines α and β are primarily related to occurrence and development of autoimmune thyroid disease. This study reviews chemokines and their receptors that are related to Graves’ disease and Hashimoto’s thyroiditis.展开更多
目的:探究血清CC趋化因子配体2(CCL2)、血管内皮生长因子(VEGFA)与非酒精性脂肪性肝病(NAFLD)患者肝纤维化和代谢综合征(MS)的关系。方法:选取2022年1月至2024年1月在本院就诊的NAFLD患者116例(NAFLD组)作为研究对象,根据患者否并发MS分...目的:探究血清CC趋化因子配体2(CCL2)、血管内皮生长因子(VEGFA)与非酒精性脂肪性肝病(NAFLD)患者肝纤维化和代谢综合征(MS)的关系。方法:选取2022年1月至2024年1月在本院就诊的NAFLD患者116例(NAFLD组)作为研究对象,根据患者否并发MS分为MS组(42例)和非MS组(74例),另取同期体检健康者66例作为对照组。收集所有受试者的临床资料;采用ELISA法检测血清中CCL2和VEGFA表达量;Pearson法分析血清CCL2、VEGFA水平与肝纤维化及MS相关指标的相关性;Logistic多因素分析影响NAFLD患者并发MS的因素;受试者工作特征曲线分析血清CCL2和VEGFA水平对NAFLD患者并发MS的预测价值。结果:NAFLD患者血清CCL2、VEGFA水平以及肝纤维化指标层黏连蛋白(LN)、透明质酸(HA)、Ⅲ型前胶原(PCⅢ)、Ⅳ型胶原(ⅣC)均显著高于对照组(P<0.05)。MS组的收缩压、舒张压、空腹血糖(FPG)、餐后2 h血糖(2 h PG)、甘油三椡(TG)、CCL2、VEGFA水平显著高于非MS组,丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)水平显著低于非MS组(P<0.05)。血清中CCL2和VEGFA水平与LN、HA、PCⅢ、ⅣC、收缩压、舒张压、FPG、2 h PG、TG呈正相关,与ALT、AST呈负相关(P<0.05)。收缩压、舒张压、FPG、2 h PG、TG、CCL2、VEGFA是影响NAFLD患者并发MS的危险因素,ALT、AST是影响NAFLD患者并发MS的保护因素(P<0.05)。血清CCL2和VEGFA水平以及联合预测NAFLD患者并发MS情况的曲线下面积分别为0.842、0.884和0.938,联合预测优于各自单独预测(Z_(联合-CCL2)=2.959、Z_(联合-VEGFA)=2.731,P=0.003、0.006)。结论:NAFLD患者血清CCL2和VEGFA水平升高,且二者与NAFLD患者肝纤维化和MS密切相关,二者联合对NAFLD患者并发MS具有较高的预测价值。展开更多
文摘Background: The allo-immune response following organ transplantation constitutes one of the main determinants concerning both short- and long- term outcomes in renal graft recipients. Chemokines and their receptors play a diversified and important role, either homeostatic or inflammatory and direct different immune-competent cell types to the allograft. While deeply studied in the last two decades, controversy persists as a result of chemokines’ pleiotropic actions. We report our analysis of CCR1, CCR3, CCR7, CCL5 and CX3CL1 expression or synthesis by graft-infiltrating cells in human kidney transplants (KTx). At the same time, we tested their robustness in diagnosing acute rejection. Methods: Fine-needle aspiration biopsies (Fnab) were performed either on days 7 or 14 post-transplantation among stable KTx and on the day of acute rejection (AR) diagnosis. Fnab cytopreparations were studied by the enzymatic avidin-biotin complex staining for CCR1, CCR3, CCR7 and CX3CL1. From another subgroup of cases, Fnab samples were cultured for 48 hours and the supernatants were analysed for CCL5 by ELISA. Results: The group of AR cases showed a significantly up-regulated expression of CCR1, CCR3, CCR7 and CX3CL1 and a significantly higher synthesis of CCL5. The positive predictive values were respectively 92%, 97%, 85%, 76% and 78% and negative predictive values were by the same order, 100%, 73%, 100%, 98% and 83%. Conclusions: Our study permits us to advance that CCR1 and CCR3 play a significant and non-redundant role in acute rejection, and it is the first report of CCR3 association with rejection, probably related to CCL5. The presence inside the graft of significant up-regulation for CCR7 surmises that part of antigen presentation may be performed there without being restricted to secondary lymphoid sites. Our results with CX3CL1 confirm other reports.
文摘Chemokines can be divided into four categories: α, β, γ, and δ. Chemokine α is related to neutrophil chemotaxis. Chemokine β is correlated with adsorption of monocytes,basophils, and eosinophils. Chemokine γ is mainly a lymphocyte chemokine. Function of chemokine δ remains unclear. Chemokines α and β are primarily related to occurrence and development of autoimmune thyroid disease. This study reviews chemokines and their receptors that are related to Graves’ disease and Hashimoto’s thyroiditis.
文摘目的:探究血清CC趋化因子配体2(CCL2)、血管内皮生长因子(VEGFA)与非酒精性脂肪性肝病(NAFLD)患者肝纤维化和代谢综合征(MS)的关系。方法:选取2022年1月至2024年1月在本院就诊的NAFLD患者116例(NAFLD组)作为研究对象,根据患者否并发MS分为MS组(42例)和非MS组(74例),另取同期体检健康者66例作为对照组。收集所有受试者的临床资料;采用ELISA法检测血清中CCL2和VEGFA表达量;Pearson法分析血清CCL2、VEGFA水平与肝纤维化及MS相关指标的相关性;Logistic多因素分析影响NAFLD患者并发MS的因素;受试者工作特征曲线分析血清CCL2和VEGFA水平对NAFLD患者并发MS的预测价值。结果:NAFLD患者血清CCL2、VEGFA水平以及肝纤维化指标层黏连蛋白(LN)、透明质酸(HA)、Ⅲ型前胶原(PCⅢ)、Ⅳ型胶原(ⅣC)均显著高于对照组(P<0.05)。MS组的收缩压、舒张压、空腹血糖(FPG)、餐后2 h血糖(2 h PG)、甘油三椡(TG)、CCL2、VEGFA水平显著高于非MS组,丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)水平显著低于非MS组(P<0.05)。血清中CCL2和VEGFA水平与LN、HA、PCⅢ、ⅣC、收缩压、舒张压、FPG、2 h PG、TG呈正相关,与ALT、AST呈负相关(P<0.05)。收缩压、舒张压、FPG、2 h PG、TG、CCL2、VEGFA是影响NAFLD患者并发MS的危险因素,ALT、AST是影响NAFLD患者并发MS的保护因素(P<0.05)。血清CCL2和VEGFA水平以及联合预测NAFLD患者并发MS情况的曲线下面积分别为0.842、0.884和0.938,联合预测优于各自单独预测(Z_(联合-CCL2)=2.959、Z_(联合-VEGFA)=2.731,P=0.003、0.006)。结论:NAFLD患者血清CCL2和VEGFA水平升高,且二者与NAFLD患者肝纤维化和MS密切相关,二者联合对NAFLD患者并发MS具有较高的预测价值。
