As fundamental components of life,biomacromolecules such as proteins,nucleic acids,and polysaccharides are constantly undergoing chemical modifications that are versatile,site-specific,and dynamic,and are often tightl...As fundamental components of life,biomacromolecules such as proteins,nucleic acids,and polysaccharides are constantly undergoing chemical modifications that are versatile,site-specific,and dynamic,and are often tightly regulated.This review summarizes recent advances in the study of dynamic modifications and chemical interventions of biomacromolecules that are supported by the major research plan(MRP)of the National Natural Science Foundation of China(NSFC).Firstly,we will introduce novel methods and techniques in chemical labeling,detection,as well as manipulation of dynamic biomacromolecular modifications.We will next discuss the molecular mechanisms and biological functions of the dynamic modifications of biomacromolecules,particularly of newly discovered modifications.Finally,we will explore chemical intervention strategies targeting these modifications and the associated disease.This review selectively highlights significant recent progress beingmade by the researchers supported byMRP,with a particular emphasis on representative chemical techniques and their biomedical applications along this exciting direction.展开更多
To evaluate in an interventional trial on knee osteoarthritis（OA） the level and change of two serum biomarkers and their correlation with imaging parameters.The previously reported interventional OA study（ClinicalT...To evaluate in an interventional trial on knee osteoarthritis（OA） the level and change of two serum biomarkers and their correlation with imaging parameters.The previously reported interventional OA study（ClinicalTrials.gov：NCT00536302） identified a positive effect of collagen hydrolysate（CH） on cartilage morphology in patients with knee OA using delayed gadolinium enhanced magnetic resonance imaging（dGEMRIC）.It was the objective in this research project to evaluate in an interventional clinical trial on knee OA the level and change of two serum biomarkers and their correlation with imaging parameters.In blood samples of study participants,we determined the concentration of procollagen type II N-terminal propeptide（PIIANP） and aggrecan chondroitin sulfate 846 epitope（CS846） at baseline（BL） and at the follow-up（FU） visits at 24 and 48 weeks.We measured the level and change of biomarker concentrations in both study groups,and the correlation of those changes with changes in dGEMRIC.For the biomarker PIIANP,we observed a significantly greater increase in the CH group（29.9%vs.1.2%at week 24,P =0.001）.For CS846,the mean concentration was lower among the CH treated participants at 24 weeks（78%vs.96%,P= 0.045）.Consistent correlations of changes in biomarkers PIIANP and CS846 with changes of the dGEMRIC score could not be observed.In this study,different changes per treatment group,CH and placebo were seen for dGEMRIC and PIIANP BL to 24 weeks FU,but only weak correlations between changes in dGEMRIC and biochemical markers.展开更多
文摘As fundamental components of life,biomacromolecules such as proteins,nucleic acids,and polysaccharides are constantly undergoing chemical modifications that are versatile,site-specific,and dynamic,and are often tightly regulated.This review summarizes recent advances in the study of dynamic modifications and chemical interventions of biomacromolecules that are supported by the major research plan(MRP)of the National Natural Science Foundation of China(NSFC).Firstly,we will introduce novel methods and techniques in chemical labeling,detection,as well as manipulation of dynamic biomacromolecular modifications.We will next discuss the molecular mechanisms and biological functions of the dynamic modifications of biomacromolecules,particularly of newly discovered modifications.Finally,we will explore chemical intervention strategies targeting these modifications and the associated disease.This review selectively highlights significant recent progress beingmade by the researchers supported byMRP,with a particular emphasis on representative chemical techniques and their biomedical applications along this exciting direction.
基金The original interventional study(NCT00536302) had been sponsored by the company Gelita and the company-specific brand of CH had been used
文摘To evaluate in an interventional trial on knee osteoarthritis（OA） the level and change of two serum biomarkers and their correlation with imaging parameters.The previously reported interventional OA study（ClinicalTrials.gov：NCT00536302） identified a positive effect of collagen hydrolysate（CH） on cartilage morphology in patients with knee OA using delayed gadolinium enhanced magnetic resonance imaging（dGEMRIC）.It was the objective in this research project to evaluate in an interventional clinical trial on knee OA the level and change of two serum biomarkers and their correlation with imaging parameters.In blood samples of study participants,we determined the concentration of procollagen type II N-terminal propeptide（PIIANP） and aggrecan chondroitin sulfate 846 epitope（CS846） at baseline（BL） and at the follow-up（FU） visits at 24 and 48 weeks.We measured the level and change of biomarker concentrations in both study groups,and the correlation of those changes with changes in dGEMRIC.For the biomarker PIIANP,we observed a significantly greater increase in the CH group（29.9%vs.1.2%at week 24,P =0.001）.For CS846,the mean concentration was lower among the CH treated participants at 24 weeks（78%vs.96%,P= 0.045）.Consistent correlations of changes in biomarkers PIIANP and CS846 with changes of the dGEMRIC score could not be observed.In this study,different changes per treatment group,CH and placebo were seen for dGEMRIC and PIIANP BL to 24 weeks FU,but only weak correlations between changes in dGEMRIC and biochemical markers.
