多囊卵巢综合征(PCOS)作为育龄期女性常见的内分泌疾病,其人群中脂肪因子被不断研究,如脂联素等在正常女性中的循环水平与PCOS人群具有差异性。随着PCOS的相关研究越来越深入,Chemerin作为一种脂肪因子与PCOS有复杂的联系。本文就Cheme...多囊卵巢综合征(PCOS)作为育龄期女性常见的内分泌疾病,其人群中脂肪因子被不断研究,如脂联素等在正常女性中的循环水平与PCOS人群具有差异性。随着PCOS的相关研究越来越深入,Chemerin作为一种脂肪因子与PCOS有复杂的联系。本文就Chemerin在PCOS中的研究进展进行综述,旨在为Chemerin在PCOS的进一步研究中提出新的见解,为了解和治疗PCOS提供新的思路。Polycystic ovary syndrome (PCOS) is a prevalent endocrine disorder among women of reproductive age and has been extensively studied in the context of adipokine dysregulation. Among these adipokines, lipocalin demonstrates distinct circulating levels in PCOS patients compared to non-PCOS individuals. As research on PCOS advances, Chemerin, a multifunctional adipokine, has gained increasing attention due to its potential role in the pathophysiology of PCOS. This article reviews the current research progress on Chemerin in PCOS, aiming to provide novel insights for future investigations and to explore potential therapeutic strategies for PCOS management.展开更多
Introduction:Human chemerin is an adipokine that regulates chemotaxis,inflammation,and glucose metabolism.In addition,accumulating evidence suggests that chemerin promotes apoptosis,autophagy,and pyroptosis.However,th...Introduction:Human chemerin is an adipokine that regulates chemotaxis,inflammation,and glucose metabolism.In addition,accumulating evidence suggests that chemerin promotes apoptosis,autophagy,and pyroptosis.However,there are no data on its impact on eryptosis.The current study aimed to analyze the effects of human active Glu^(21)-Ser^(157) chemerin on eryptosis in vitro.Materials and Methods:Human chemerin 0-2-10-50μg/mL was incubated for 24 h with human erythrocytes(hematocrit 0.4%)obtained from eight healthy individuals.Flow cytometry-based determination of phospholipid scrambling,reactive oxygen species(ROS)production,and intracellular Ca^(2+)levels was performed.To supplement data on ROS and Ca^(2+)signaling in chemerin-mediated eryptosis,incubation in the presence or absence of antioxidants vitamin C and N-acetylcysteine and Ca^(2+)-binding agent EGTA was carried out,respectively.Confocal microscopy-based techniques were used to detect reactive nitrogen species(RNS)generation,involvement of caspase-3 and caspase-8,as well as the state of lipid order in cell membranes of erythrocytes exposed to human Glu^(21)-Ser^(157) chemerin.Results:Our observations suggest that human Glu^(21)-Ser^(157) chemerin had no impact on eryptosis parameters at 2μg/mL.However,chemerin stimulated phosphatidylserine externalization,ROS production,and Ca^(2+)accumulation at higher concentrations suggesting activation of eryptosis.Ca^(2+)uptake turned out to be at least partly required for chemerin-mediated eryptosis.Chemerin-mediated erythrotoxicity was additionally mediated by RNS,caspase-3,and caspase-8.Moreover,Glu^(21)-Ser^(157) chemerin promoted reduction in the liquid-ordered phase of cell membranes in erythrocytes.Conclusions:The present study first discloses that human chemerin can induce eryptosis via Ca^(2+)-dependent mechanisms at concentrations noticeably exceeding circulating levels.Thus,chemerin-induced eryptosis can hardly contribute to eryptosis-mediated anemia in diseases associated with enhanced levels of chemerin in blood.展开更多
文摘多囊卵巢综合征(PCOS)作为育龄期女性常见的内分泌疾病,其人群中脂肪因子被不断研究,如脂联素等在正常女性中的循环水平与PCOS人群具有差异性。随着PCOS的相关研究越来越深入,Chemerin作为一种脂肪因子与PCOS有复杂的联系。本文就Chemerin在PCOS中的研究进展进行综述,旨在为Chemerin在PCOS的进一步研究中提出新的见解,为了解和治疗PCOS提供新的思路。Polycystic ovary syndrome (PCOS) is a prevalent endocrine disorder among women of reproductive age and has been extensively studied in the context of adipokine dysregulation. Among these adipokines, lipocalin demonstrates distinct circulating levels in PCOS patients compared to non-PCOS individuals. As research on PCOS advances, Chemerin, a multifunctional adipokine, has gained increasing attention due to its potential role in the pathophysiology of PCOS. This article reviews the current research progress on Chemerin in PCOS, aiming to provide novel insights for future investigations and to explore potential therapeutic strategies for PCOS management.
基金supported by the National Natural Science Foundation of China(No.82111530101,81803493)the Outstanding Youth Foundation of Jiangxi Province,China(No.20212ACB216002)the Young Jinggang Scholars Award Program of Jiangxi Province,China(No.QNJG2019089)。
文摘Introduction:Human chemerin is an adipokine that regulates chemotaxis,inflammation,and glucose metabolism.In addition,accumulating evidence suggests that chemerin promotes apoptosis,autophagy,and pyroptosis.However,there are no data on its impact on eryptosis.The current study aimed to analyze the effects of human active Glu^(21)-Ser^(157) chemerin on eryptosis in vitro.Materials and Methods:Human chemerin 0-2-10-50μg/mL was incubated for 24 h with human erythrocytes(hematocrit 0.4%)obtained from eight healthy individuals.Flow cytometry-based determination of phospholipid scrambling,reactive oxygen species(ROS)production,and intracellular Ca^(2+)levels was performed.To supplement data on ROS and Ca^(2+)signaling in chemerin-mediated eryptosis,incubation in the presence or absence of antioxidants vitamin C and N-acetylcysteine and Ca^(2+)-binding agent EGTA was carried out,respectively.Confocal microscopy-based techniques were used to detect reactive nitrogen species(RNS)generation,involvement of caspase-3 and caspase-8,as well as the state of lipid order in cell membranes of erythrocytes exposed to human Glu^(21)-Ser^(157) chemerin.Results:Our observations suggest that human Glu^(21)-Ser^(157) chemerin had no impact on eryptosis parameters at 2μg/mL.However,chemerin stimulated phosphatidylserine externalization,ROS production,and Ca^(2+)accumulation at higher concentrations suggesting activation of eryptosis.Ca^(2+)uptake turned out to be at least partly required for chemerin-mediated eryptosis.Chemerin-mediated erythrotoxicity was additionally mediated by RNS,caspase-3,and caspase-8.Moreover,Glu^(21)-Ser^(157) chemerin promoted reduction in the liquid-ordered phase of cell membranes in erythrocytes.Conclusions:The present study first discloses that human chemerin can induce eryptosis via Ca^(2+)-dependent mechanisms at concentrations noticeably exceeding circulating levels.Thus,chemerin-induced eryptosis can hardly contribute to eryptosis-mediated anemia in diseases associated with enhanced levels of chemerin in blood.
