Microglia-mediated neuroinflammation plays a crucial role in ischemic stroke;consequently,understanding its regulation could facilitate the development of therapies for ischemic stroke.Chemerin 15,a 15-amino acid pept...Microglia-mediated neuroinflammation plays a crucial role in ischemic stroke;consequently,understanding its regulation could facilitate the development of therapies for ischemic stroke.Chemerin 15,a 15-amino acid peptide derived from chemerin,exerts powerful anti-inflammatory effects through ChemR23,modulates macrophage polarization,and diminishes inflammatory cytokine expression in peripheral inflammation models.However,its effects on microglia and stroke remain unclear.In this study,we used an in vitro oxygen/glucose deprivation BV2 cell model and a mouse model of ischemia-reperfusion injury to investigate the role of chemerin 15 in stroke and the underlying mechanisms.We co-cultured BV2 microglial cells with HT-22 hippocampal neurons and observed that chemerin 15 reduced apoptosis in HT-22 cells.Furthermore,we found that chemerin 15 binds to the ChemR23 receptor on the cell surface,inducing its internalization.This process regulated the activity of adenosine 5ʹ-monophosphate-activated protein kinase and inhibited its downstream target nuclear factor kappa B.These effects could be reversed by treatment withα-NETA,a ChemR23 inhibitor.In mice with ischemia-reperfusion injury,chemerin 15 modulated microglial polarization,reduced infarct volume and neuronal apoptosis,and facilitated cognitive and neurological function recovery.Our findings suggest that chemerin 15 suppresses the microglia-mediated inflammatory response,decreases neuronal apoptosis,and enhances long-term neurological function recovery by inducing ChemR23 internalization and regulating the adenosine 5ʹ-monophosphate-activated protein kinase/nuclear factor kappa B signaling pathway.展开更多
目的探讨杞菊地黄汤联合维生素D_(3)对妊娠糖尿病血清趋化素(Chemerin)、锌α2糖蛋白(zinc-α2-glycoprotein,ZAG)及妊娠结局的影响。方法选取2022年10月至2024年2月杭州市富阳区第一人民医院收治的77例妊娠糖尿病患者,根据随机数字表...目的探讨杞菊地黄汤联合维生素D_(3)对妊娠糖尿病血清趋化素(Chemerin)、锌α2糖蛋白(zinc-α2-glycoprotein,ZAG)及妊娠结局的影响。方法选取2022年10月至2024年2月杭州市富阳区第一人民医院收治的77例妊娠糖尿病患者,根据随机数字表法将其分为对照组(n=38)和观察组(n=39)。对照组应用常规治疗,观察组应用杞菊地黄汤联合维生素D_(3)治疗,均持续治疗4周。比较两组患者的疗效、血糖、Chemerin、ZAG、1,25-二羟维生素D_(3)[1,25-dihydroxy vitamin D_(3),1,25(OH)_(2)D_(3)]水平、胰岛β细胞功能指数(homeostasis model assessment forβcell function,HOMA-β)、稳态模型评估的胰岛素抵抗(homeostasis model assessment of insulin resistance,HOMA-IR)指数、中医证候积分和不良妊娠结局。结果观察组患者的总有效率显著高于对照组(χ^(2)=5.845,P=0.016)。治疗后,观察组患者的空腹血糖、餐后2h血糖水平、HOMA-IR指数、Chemerin水平、中医证候积分均显著低于对照组,HOMA-β、ZAG、1,25(OH)_(2)D_(3)水平显著高于对照组(P<0.05)。观察组患者的不良妊娠结局发生率显著低于对照组(χ^(2)=4.073,P=0.044)。结论杞菊地黄汤联合维生素D_(3)可显著改善妊娠糖尿病患者的血糖水平、胰岛素抵抗,降低血清Chemerin水平,增高血清ZAG水平,且能减少不良妊娠结局。展开更多
多囊卵巢综合征(PCOS)作为育龄期女性常见的内分泌疾病,其人群中脂肪因子被不断研究,如脂联素等在正常女性中的循环水平与PCOS人群具有差异性。随着PCOS的相关研究越来越深入,Chemerin作为一种脂肪因子与PCOS有复杂的联系。本文就Cheme...多囊卵巢综合征(PCOS)作为育龄期女性常见的内分泌疾病,其人群中脂肪因子被不断研究,如脂联素等在正常女性中的循环水平与PCOS人群具有差异性。随着PCOS的相关研究越来越深入,Chemerin作为一种脂肪因子与PCOS有复杂的联系。本文就Chemerin在PCOS中的研究进展进行综述,旨在为Chemerin在PCOS的进一步研究中提出新的见解,为了解和治疗PCOS提供新的思路。Polycystic ovary syndrome (PCOS) is a prevalent endocrine disorder among women of reproductive age and has been extensively studied in the context of adipokine dysregulation. Among these adipokines, lipocalin demonstrates distinct circulating levels in PCOS patients compared to non-PCOS individuals. As research on PCOS advances, Chemerin, a multifunctional adipokine, has gained increasing attention due to its potential role in the pathophysiology of PCOS. This article reviews the current research progress on Chemerin in PCOS, aiming to provide novel insights for future investigations and to explore potential therapeutic strategies for PCOS management.展开更多
目的·探讨中国汉族人群中chemerin及其受体chemerin R与肥胖及2型糖尿病的关系。方法·收集24名腹部择期手术患者作为研究对象,按照体质量指数(BMI)分为正常糖调节正常体重组(NGR-NW)、正常糖调节超重/肥胖组(NGR-OW/OB)和2型...目的·探讨中国汉族人群中chemerin及其受体chemerin R与肥胖及2型糖尿病的关系。方法·收集24名腹部择期手术患者作为研究对象,按照体质量指数(BMI)分为正常糖调节正常体重组(NGR-NW)、正常糖调节超重/肥胖组(NGR-OW/OB)和2型糖尿病超重/肥胖组(T2DM-OW/OB)。采用RT-PCR法测定腹部皮下脂肪组织和腹内网膜脂肪组织中chemerin、chemerin R m RNA水平。结果·NGR-OW/OB组和T2DM-OW/OB组腹部皮下脂肪组织和腹内网膜脂肪组织中chemerin m RNA水平均显著高于NGR-NW组(P<0.05)。相关分析显示,网膜脂肪组织chemerin m RNA水平与BMI、空腹胰岛素(FINS)、三酰甘油和血清chemerin浓度呈正相关(r分别为0.577、0.561、0.472、0.623,P均<0.05);腹部皮下脂肪组织中chemerin m RNA水平与BMI、FINS、血清chemerin浓度呈正相关(r分别为0.692、0.513、0.497,P均<0.05)。结论·网膜脂肪组织、皮下脂肪组织的chemerin m RNA的表达量均与BMI、FINS、血清chemerin浓度呈正相关,提示chemerin基因可能在肥胖及2型糖尿病的病理生理机制中发挥一定作用。展开更多
基金Gulin-Southwest Medical University Hospital Strategic Cooperation Project,No.2022 GLXNYDFY10(to JL)Suining First People’s Hospital-Southwest Medical University Cooperation Project,No.2021SNXNYD06(to JL)+2 种基金Postdoctoral Research Foundation of China,No.2021M692285(to LW)Key Research and Development Project of the Science and Technology Department of Sichuan Province,No.2022YFS0318(to LW)Post-Doctor Research Project of West China Hospital,Sichuan University,No.2021HXBH083(to LW).
文摘Microglia-mediated neuroinflammation plays a crucial role in ischemic stroke;consequently,understanding its regulation could facilitate the development of therapies for ischemic stroke.Chemerin 15,a 15-amino acid peptide derived from chemerin,exerts powerful anti-inflammatory effects through ChemR23,modulates macrophage polarization,and diminishes inflammatory cytokine expression in peripheral inflammation models.However,its effects on microglia and stroke remain unclear.In this study,we used an in vitro oxygen/glucose deprivation BV2 cell model and a mouse model of ischemia-reperfusion injury to investigate the role of chemerin 15 in stroke and the underlying mechanisms.We co-cultured BV2 microglial cells with HT-22 hippocampal neurons and observed that chemerin 15 reduced apoptosis in HT-22 cells.Furthermore,we found that chemerin 15 binds to the ChemR23 receptor on the cell surface,inducing its internalization.This process regulated the activity of adenosine 5ʹ-monophosphate-activated protein kinase and inhibited its downstream target nuclear factor kappa B.These effects could be reversed by treatment withα-NETA,a ChemR23 inhibitor.In mice with ischemia-reperfusion injury,chemerin 15 modulated microglial polarization,reduced infarct volume and neuronal apoptosis,and facilitated cognitive and neurological function recovery.Our findings suggest that chemerin 15 suppresses the microglia-mediated inflammatory response,decreases neuronal apoptosis,and enhances long-term neurological function recovery by inducing ChemR23 internalization and regulating the adenosine 5ʹ-monophosphate-activated protein kinase/nuclear factor kappa B signaling pathway.
文摘目的探讨杞菊地黄汤联合维生素D_(3)对妊娠糖尿病血清趋化素(Chemerin)、锌α2糖蛋白(zinc-α2-glycoprotein,ZAG)及妊娠结局的影响。方法选取2022年10月至2024年2月杭州市富阳区第一人民医院收治的77例妊娠糖尿病患者,根据随机数字表法将其分为对照组(n=38)和观察组(n=39)。对照组应用常规治疗,观察组应用杞菊地黄汤联合维生素D_(3)治疗,均持续治疗4周。比较两组患者的疗效、血糖、Chemerin、ZAG、1,25-二羟维生素D_(3)[1,25-dihydroxy vitamin D_(3),1,25(OH)_(2)D_(3)]水平、胰岛β细胞功能指数(homeostasis model assessment forβcell function,HOMA-β)、稳态模型评估的胰岛素抵抗(homeostasis model assessment of insulin resistance,HOMA-IR)指数、中医证候积分和不良妊娠结局。结果观察组患者的总有效率显著高于对照组(χ^(2)=5.845,P=0.016)。治疗后,观察组患者的空腹血糖、餐后2h血糖水平、HOMA-IR指数、Chemerin水平、中医证候积分均显著低于对照组,HOMA-β、ZAG、1,25(OH)_(2)D_(3)水平显著高于对照组(P<0.05)。观察组患者的不良妊娠结局发生率显著低于对照组(χ^(2)=4.073,P=0.044)。结论杞菊地黄汤联合维生素D_(3)可显著改善妊娠糖尿病患者的血糖水平、胰岛素抵抗,降低血清Chemerin水平,增高血清ZAG水平,且能减少不良妊娠结局。
文摘多囊卵巢综合征(PCOS)作为育龄期女性常见的内分泌疾病,其人群中脂肪因子被不断研究,如脂联素等在正常女性中的循环水平与PCOS人群具有差异性。随着PCOS的相关研究越来越深入,Chemerin作为一种脂肪因子与PCOS有复杂的联系。本文就Chemerin在PCOS中的研究进展进行综述,旨在为Chemerin在PCOS的进一步研究中提出新的见解,为了解和治疗PCOS提供新的思路。Polycystic ovary syndrome (PCOS) is a prevalent endocrine disorder among women of reproductive age and has been extensively studied in the context of adipokine dysregulation. Among these adipokines, lipocalin demonstrates distinct circulating levels in PCOS patients compared to non-PCOS individuals. As research on PCOS advances, Chemerin, a multifunctional adipokine, has gained increasing attention due to its potential role in the pathophysiology of PCOS. This article reviews the current research progress on Chemerin in PCOS, aiming to provide novel insights for future investigations and to explore potential therapeutic strategies for PCOS management.
文摘目的·探讨中国汉族人群中chemerin及其受体chemerin R与肥胖及2型糖尿病的关系。方法·收集24名腹部择期手术患者作为研究对象,按照体质量指数(BMI)分为正常糖调节正常体重组(NGR-NW)、正常糖调节超重/肥胖组(NGR-OW/OB)和2型糖尿病超重/肥胖组(T2DM-OW/OB)。采用RT-PCR法测定腹部皮下脂肪组织和腹内网膜脂肪组织中chemerin、chemerin R m RNA水平。结果·NGR-OW/OB组和T2DM-OW/OB组腹部皮下脂肪组织和腹内网膜脂肪组织中chemerin m RNA水平均显著高于NGR-NW组(P<0.05)。相关分析显示,网膜脂肪组织chemerin m RNA水平与BMI、空腹胰岛素(FINS)、三酰甘油和血清chemerin浓度呈正相关(r分别为0.577、0.561、0.472、0.623,P均<0.05);腹部皮下脂肪组织中chemerin m RNA水平与BMI、FINS、血清chemerin浓度呈正相关(r分别为0.692、0.513、0.497,P均<0.05)。结论·网膜脂肪组织、皮下脂肪组织的chemerin m RNA的表达量均与BMI、FINS、血清chemerin浓度呈正相关,提示chemerin基因可能在肥胖及2型糖尿病的病理生理机制中发挥一定作用。
