One hundred and five patients with primary unipolar depression were randomly divided into three groups： drug group （Seroxat administration）, acupuncture group （Seroxat plus acupunc- ture）, and electroacupuncture ...One hundred and five patients with primary unipolar depression were randomly divided into three groups： drug group （Seroxat administration）, acupuncture group （Seroxat plus acupunc- ture）, and electroacupuncture group （Seroxat plus acupuncture plus electroacupuncture）. Patients＇ symptoms were evaluated using a psychometric questionnaire, the Symptom Check- list-90, before intervention and after 2, 4, 6 and 10 weeks of treatment. The individual factor scores and the total score from the Symptom Checldist-90 reduced in all three groups as treat- ment progressed. In the acupuncture and electroacupuncture groups, the total score and the factor scores for obsessive-compulsive symptoms, depression, and anxiety were significantly lower than those in the drug group. There was no significant difference in the factor scores or total scores between the acupuncture and electroacupuncture groups. Some factor scores in the electroacupuncture group, such as somatization, depression, hostility, and phobic anxiety, were increased at 10 weeks compared with the respective score immediately after the course of electroacupuncture at 6 weeks. Our findings indicate that administration of Seroxat alone or in combination with acupuncture/electroacupuncture can produce a significant effect in patients with primary unipolar depression. Furthermore, acupuncture/electroacupuncture has a rapid onset of therapeutic effect and produces a noticeable improvement in obsessive-compulsive, de- pressive and anxiety symptoms.展开更多
【目的】探究黑龙江林蛙(Rana amurensis)热休克蛋白90α家族B类成员1(heat shock protein 90 alpha class B member 1,HSP90AB1)基因的序列特征,了解其组织表达特性和脂多糖(LPS)诱导下的表达变化。【方法】以黑龙江林蛙脾脏组织cDNA...【目的】探究黑龙江林蛙(Rana amurensis)热休克蛋白90α家族B类成员1(heat shock protein 90 alpha class B member 1,HSP90AB1)基因的序列特征,了解其组织表达特性和脂多糖(LPS)诱导下的表达变化。【方法】以黑龙江林蛙脾脏组织cDNA为模板,通过PCR扩增HSP90AB1基因完整编码区(CDS)序列,使用在线软件对其编码蛋白进行生物信息学分析;利用实时荧光定量PCR和Western blotting检测生理状态下HSP90AB1基因在黑龙江林蛙皮肤、肌肉、心脏、肝脏、脾脏、肺脏、肾脏和胃等组织器官中的表达量,同时对LPS诱导下不同时间点HSP90AB1基因在不同组织器官的转录水平进行检测。【结果】黑龙江林蛙HSP90AB1基因CDS长2178 bp,共编码725个氨基酸。生物信息学分析显示,HSP90AB1蛋白不存在信号肽且无跨膜信号,属于非跨膜蛋白,存在62个潜在磷酸化位点(含33个丝氨酸、19个苏氨酸及10个酪氨酸位点),其二级结构以α-螺旋为主(占47.03%),其次为无规则卷曲(占39.59%)和延伸链(占13.38%)。系统进化树结果显示,黑龙江林蛙与欧洲林蛙亲缘关系最近。在生理状态下,HSP90AB1基因在黑龙江林蛙胃中表达量最高,且显著高于其他组织(P<0.05);在肌肉组织中表达量最低,且显著低于其他组织(P<0.05)。Western blotting结果显示,HSP90AB1蛋白在黑龙江林蛙胃中表达量最高;在心脏中表达量最低,且显著低于其他组织(P<0.05)。LPS诱导后,黑龙江林蛙各组织中HSP90AB1基因表达量随诱导时间延长而呈降低趋势,其中诱导72和96 h表达量均显著低于其他时间点(P<0.05);诱导12、72 h时脾脏中HSP90AB1基因表达量显著高于其他组织(P<0.05);24 h时肌肉和脾脏中HSP90AB1基因表达量显著高于其他组织(P<0.05)。【结论】本研究成功克隆了黑龙江林蛙HSP90AB1基因CDS区序列,并明确了其在不同组织中的表达规律,其中在胃中表达量最高,肌肉组织中表达量最低。LPS诱导可明显抑制HSP90AB1基因在黑龙江林蛙不同组织中的转录水平。本试验结果为深入探究HSP90AB1基因在林蛙生理及应激过程中的功能机制提供了理论依据。展开更多
热激蛋白90(heat shock protein 90,HSP90)是一类结构保守且在免疫过程中具有重要功能的蛋白质。此前已从厚壳贻贝(Mytilus coruscus)血清中鉴定到一种分泌型HSP90(Mc-HSP90-S)。为了解该蛋白质在贻贝先天免疫系统中的生物学功能,对Mc-H...热激蛋白90(heat shock protein 90,HSP90)是一类结构保守且在免疫过程中具有重要功能的蛋白质。此前已从厚壳贻贝(Mytilus coruscus)血清中鉴定到一种分泌型HSP90(Mc-HSP90-S)。为了解该蛋白质在贻贝先天免疫系统中的生物学功能,对Mc-HSP90-S开展了序列特征、表达模式、原核重组表达以及表达产物体外活性分析。结果表明,Mc-HSP90-S由797个氨基酸残基组成,理论分子量为91.68 kD,理论pI为4.89。Mc-HSP90-S在贻贝免疫相关组织中具有相对高表达,热胁迫和微生物胁迫均可显著上调Mc-HSP90-S的基因表达量(P<0.05)。重组Mc-HSP90-S蛋白在体外表现出明显的抑菌活性和选择性微生物凝集活性,其机制可能在于通过消耗ATP而对细菌生长产生抑制。此外,重组Mc-HSP90-S可显著上调贻贝免疫信号相关基因TLR4在鳃组织的相对表达量(P<0.05)。上述结果为深入了解分泌型HSP90在贻贝中的免疫和分子功能奠定了基础。展开更多
肝细胞癌(hepatocellular carcinoma,HCC)是全球常见的恶性肿瘤,其治疗策略多样,其中钇-90微球选择性内放射治疗(Yttrium-90 microsphere selective internal radiation therapy,^(90)Y-SIRT)作为一种重要的局部治疗方式,在HCC的综合管...肝细胞癌(hepatocellular carcinoma,HCC)是全球常见的恶性肿瘤,其治疗策略多样,其中钇-90微球选择性内放射治疗(Yttrium-90 microsphere selective internal radiation therapy,^(90)Y-SIRT)作为一种重要的局部治疗方式,在HCC的综合管理中扮演着日益重要的角色。近年来,随着临床应用的深入,^(90)Y-SIRT在HCC治疗中积累了丰富的循证医学证据。在此背景下,美国国家综合癌症网络(National Comprehensive Cancer Network,NCCN)、欧洲肝脏学会(European Association for the Study of the Liver,EASL)、欧洲肿瘤内科学会(European Society for Medical Oncology,ESMO)、英国胃肠病学会(British Society of Gastroenterology,BSG)相继更新了HCC诊疗指南,并将^(90)Y-SIRT纳入治疗推荐体系。本文将以这些国际指南的推荐意见为核心,结合最新临床研究与临床实践经验,系统阐述^(90)Y-SIRT在HCC治疗中的应用价值。展开更多
基金supported by the National Science and Technology Support Program of China,No.2006BAI12B05-2the National Natural Science Foundation of China,No.30672768Key Discipline Construction Project of"211 Project"of Guangdong Province in China
文摘One hundred and five patients with primary unipolar depression were randomly divided into three groups： drug group （Seroxat administration）, acupuncture group （Seroxat plus acupunc- ture）, and electroacupuncture group （Seroxat plus acupuncture plus electroacupuncture）. Patients＇ symptoms were evaluated using a psychometric questionnaire, the Symptom Check- list-90, before intervention and after 2, 4, 6 and 10 weeks of treatment. The individual factor scores and the total score from the Symptom Checldist-90 reduced in all three groups as treat- ment progressed. In the acupuncture and electroacupuncture groups, the total score and the factor scores for obsessive-compulsive symptoms, depression, and anxiety were significantly lower than those in the drug group. There was no significant difference in the factor scores or total scores between the acupuncture and electroacupuncture groups. Some factor scores in the electroacupuncture group, such as somatization, depression, hostility, and phobic anxiety, were increased at 10 weeks compared with the respective score immediately after the course of electroacupuncture at 6 weeks. Our findings indicate that administration of Seroxat alone or in combination with acupuncture/electroacupuncture can produce a significant effect in patients with primary unipolar depression. Furthermore, acupuncture/electroacupuncture has a rapid onset of therapeutic effect and produces a noticeable improvement in obsessive-compulsive, de- pressive and anxiety symptoms.
文摘【目的】探究黑龙江林蛙(Rana amurensis)热休克蛋白90α家族B类成员1(heat shock protein 90 alpha class B member 1,HSP90AB1)基因的序列特征,了解其组织表达特性和脂多糖(LPS)诱导下的表达变化。【方法】以黑龙江林蛙脾脏组织cDNA为模板,通过PCR扩增HSP90AB1基因完整编码区(CDS)序列,使用在线软件对其编码蛋白进行生物信息学分析;利用实时荧光定量PCR和Western blotting检测生理状态下HSP90AB1基因在黑龙江林蛙皮肤、肌肉、心脏、肝脏、脾脏、肺脏、肾脏和胃等组织器官中的表达量,同时对LPS诱导下不同时间点HSP90AB1基因在不同组织器官的转录水平进行检测。【结果】黑龙江林蛙HSP90AB1基因CDS长2178 bp,共编码725个氨基酸。生物信息学分析显示,HSP90AB1蛋白不存在信号肽且无跨膜信号,属于非跨膜蛋白,存在62个潜在磷酸化位点(含33个丝氨酸、19个苏氨酸及10个酪氨酸位点),其二级结构以α-螺旋为主(占47.03%),其次为无规则卷曲(占39.59%)和延伸链(占13.38%)。系统进化树结果显示,黑龙江林蛙与欧洲林蛙亲缘关系最近。在生理状态下,HSP90AB1基因在黑龙江林蛙胃中表达量最高,且显著高于其他组织(P<0.05);在肌肉组织中表达量最低,且显著低于其他组织(P<0.05)。Western blotting结果显示,HSP90AB1蛋白在黑龙江林蛙胃中表达量最高;在心脏中表达量最低,且显著低于其他组织(P<0.05)。LPS诱导后,黑龙江林蛙各组织中HSP90AB1基因表达量随诱导时间延长而呈降低趋势,其中诱导72和96 h表达量均显著低于其他时间点(P<0.05);诱导12、72 h时脾脏中HSP90AB1基因表达量显著高于其他组织(P<0.05);24 h时肌肉和脾脏中HSP90AB1基因表达量显著高于其他组织(P<0.05)。【结论】本研究成功克隆了黑龙江林蛙HSP90AB1基因CDS区序列,并明确了其在不同组织中的表达规律,其中在胃中表达量最高,肌肉组织中表达量最低。LPS诱导可明显抑制HSP90AB1基因在黑龙江林蛙不同组织中的转录水平。本试验结果为深入探究HSP90AB1基因在林蛙生理及应激过程中的功能机制提供了理论依据。
文摘肝细胞癌(hepatocellular carcinoma,HCC)是全球常见的恶性肿瘤,其治疗策略多样,其中钇-90微球选择性内放射治疗(Yttrium-90 microsphere selective internal radiation therapy,^(90)Y-SIRT)作为一种重要的局部治疗方式,在HCC的综合管理中扮演着日益重要的角色。近年来,随着临床应用的深入,^(90)Y-SIRT在HCC治疗中积累了丰富的循证医学证据。在此背景下,美国国家综合癌症网络(National Comprehensive Cancer Network,NCCN)、欧洲肝脏学会(European Association for the Study of the Liver,EASL)、欧洲肿瘤内科学会(European Society for Medical Oncology,ESMO)、英国胃肠病学会(British Society of Gastroenterology,BSG)相继更新了HCC诊疗指南,并将^(90)Y-SIRT纳入治疗推荐体系。本文将以这些国际指南的推荐意见为核心,结合最新临床研究与临床实践经验,系统阐述^(90)Y-SIRT在HCC治疗中的应用价值。
