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Development of Sequence Characterized Amplified Region (SCAR) Primers for the Detection of Resistance to Sporisorium reiliana in Maize 被引量:4
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作者 SHI Hong-liang LI Xin-hai +5 位作者 ZHANG De-gui XIE Chuan-xiao HAO Zhuan-fang LI Ming-shun PAN Guang-tang ZHANG Sbi-huang 《Agricultural Sciences in China》 CAS CSCD 2009年第8期910-919,共10页
Head smut of maize (Zea mays L.), which was caused by Sporisorium reiliana, occurred in most of the maize growing areas of the world. The purpose of this study was to develop SCAR markers for map-based cloning of re... Head smut of maize (Zea mays L.), which was caused by Sporisorium reiliana, occurred in most of the maize growing areas of the world. The purpose of this study was to develop SCAR markers for map-based cloning of resistance genes and MAS. Two sets of BC3 progenies, one (BC3Q) derived from the cross Qi319 (resistance)×Huangzao 4 (susceptible), the other (BC3M) from Mol7 (resistance)× Huangzao 4 (susceptible), were generated. Huangzao 4 was the recurrent parent in both progenies. A combination of BSA (bulked segregant analysis) with AFLP (amplified fragment length polymorphism) method was applied to map the genes involving the resistance to S. reiliana, and corresponding resistant and susceptible bulks and their parental lines were used for screening polymorphic AFLP primer pairs. One fragment of PI3M61-152 was converted into SCAR (sequence charactered amplified fragment) marker S130. The marker was mapped at chromosome bin 2.09, the interval of a major QTL region previously reported to contribute to S. reiliana resistance. Furthermore, S130 was highly and facilitate map-based cloni associated with resistance to S. reiliana, and could be useful for marker-assisted selection ng of resistance genes. 展开更多
关键词 maize (Zea mays L.) Sporisorium reiliana bulked segregant analysis amplified fragment length polymorphism sequence characterized amplified region
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Rapid detection of self-biting disease of mink by specific sequence-characterized amplified regions 被引量:2
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作者 LIU Zong-yue NING Fang-yong YANG Hong-yan ~ WEI Lai BAI Xiu-juan 《Journal of Forestry Research》 SCIE CAS CSCD 2011年第1期123-126,共4页
Self-biting disease occurred in most farmed fur animals in the world. The mechanism and rapid detection method of this disease has not been reported. We applied bulked sergeant analysis (BSA) in combination with RAP... Self-biting disease occurred in most farmed fur animals in the world. The mechanism and rapid detection method of this disease has not been reported. We applied bulked sergeant analysis (BSA) in combination with RAPD method to analyze a molecular genetic marker linked with self-biting trait in mink group. The molecular marker was converted into sequence-characterized amplified regions (SCAR) marker for rapid detection of this disease. A single RAPD marker A8 amplified a specific band of 263bp in self-biting minks, which was designated as SRA8-250, and non-specific band of 315bp in both self-biting and healthy minks. The sequences of the bands exhibited 75% and 88% similarity to Canis familiarizes major histocompatibility complex (MHC) class II region and Macaca mulatta MHC class I region, respectively. A SCAR marker SCAR-A8 was designed for the specific fragment SRA8-250 and validated in 30 self-biting minks and 30 healthy minks. Positive amplification of SCAR-A8 was detected in 24 self-biting minks and 12 healthy minks. χ2 test showed significant difference (p〈0.01) in the detection rate between the two groups. This indicated that SRA8-250 can be used as a positive marker to detect self-biting disease in minks. Furthermore, the finding that self-biting disease links with MHC genes has significant implications for the mechanism of the disease. 展开更多
关键词 MINK random amplified polymorphic DNA self-biting sequence characterized amplified region
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Panax ginseng-specific sequence characterized amplified region (SCAR) marker for testing medicinal products 被引量:1
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作者 蒋秋桃 刘丽 +6 位作者 肖炳燚 李文莉 罗晖明 聂平 丁野 李洁 李文章 《Journal of Central South University》 SCIE EI CAS CSCD 2018年第5期1052-1062,共11页
To screen genetic polymorphisms of Panax ginseng, as well as those of Panax quinquefolium and Panax notoginseng, analysis of random amplified polymorphic DNA (RAPD) was performed using 120 random primers. Of the suc... To screen genetic polymorphisms of Panax ginseng, as well as those of Panax quinquefolium and Panax notoginseng, analysis of random amplified polymorphic DNA (RAPD) was performed using 120 random primers. Of the successful amplicons obtained, the Panax ginseng-specific RAPD marker C-12 was cloned into a TA vector and sequenced (Genl3ank access number KU553472). Based on the sequence analysis results, a pair of primers specific to C-12 was designed. Finally, a SCAR marker-based identification system for Panax ginseng was developed after optimization of the reaction conditions. Using this method, two positive bands were stably observed at 300 bp and 130 bp in 33 batches of Panax ginseng samples tested, while negative results were obtained for another 101 batches of samples, including Panax quinquefolium, Panax notoginseng, adulterants, and other medicinal herbs. Thus, we successfully developed a PCR-based method for rapid and effective identification of Panax ginseng, which can be effectively used for the protection and utilization of germplasm resources and identification of the origins of Panax ginseng samples. 展开更多
关键词 Panax ginseng random amplified polymorphic DNA (RAPD) sequence characterized amplified regions(SCAR) molecular identification
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A New Type of Multielements-Doped,Carbon-based Materials Characterized by High-thermoconductiv-ity,Low Chemical Sputtering,Low RES Yield and Exposure to Plasma
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作者 许增裕 刘翔 +4 位作者 谌继明 王明旭 宋进仁 翟更太 李承新 《Plasma Science and Technology》 SCIE EI CAS CSCD 2002年第3期1311-1317,共7页
Low-Z materials, such as carbon-based materials and Be, are major plasma-facing material (PFM) for current, even in future fusion devices. In this paper, a new type of multielement-doped carbon-based materials develop... Low-Z materials, such as carbon-based materials and Be, are major plasma-facing material (PFM) for current, even in future fusion devices. In this paper, a new type of multielement-doped carbon-based materials developed are presented along with experimental re-sults of their properties. The results indicate a decrease in chemical sputtering yield by one order of magnitude, a decrease in both thermal shock resistance and radiation-enhanced sublimation, an evidently lower temperature desorption spectrum, and combined properties of exposing to plasma. 展开更多
关键词 than GBS RES A New Type of Multielements-Doped Carbon-based Materials characterized by High-thermoconductiv-ity Low Chemical Sputtering Low RES Yield and Exposure to Plasma
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Evaluation of Growth Performance of a Genetically Characterized F1 Rainbow Trout,Oncorhynchus mykiss in a Recirculating Aquaculture System
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作者 Jose Luis Arredondo-Figueroa Nayeli Ismene Armendáriz-Sáenz +2 位作者 Laura Georgina Núnez-García Jesús Trinidad Ponce-Palafox Irene De Los Angeles Barriga-Sosa 《Agricultural Sciences》 2017年第7期716-728,共13页
The growth performance of a selected F1 rainbow trout genetically characterized as polymorphic with locus Omy207UoG (NA = 13, PIC = 0.891, HE = 0.9003) was evaluated in a recirculating aquaculture system (RAS). A set ... The growth performance of a selected F1 rainbow trout genetically characterized as polymorphic with locus Omy207UoG (NA = 13, PIC = 0.891, HE = 0.9003) was evaluated in a recirculating aquaculture system (RAS). A set of 157 rainbow trout of 37.8 g of mean body weight and 14.6 cm mean total length per tank, were introduced in each of six fish tank of 5 m3 connected to a recirculating aquaculture system (RAS). The trout was rearing during 317 days. Growth performance indicators and water quality were evaluated during the experiment. The genetically selected F1 rainbow trout showed a tendency towards homogeneity in growth performance along the culture period, reaching a mean total weight of 552.2 g. The length-weight relationship indicated an isometric growth (>3.0). This study presents the basis to establish a long-term marker-assisted selection program for rainbow trout culture in a subtropical region. 展开更多
关键词 Rainbow Trout Oncorhynchus mykiss Genetically characterized GROWTH PERFORMANCE Water Quality
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Research Characterized the Physical Properties of Recycled Aggregate of Civil Construction Wastes
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作者 Ronaldo Alves de Medeiros Junior Stela Paulino Fucale Alexandre Duarte Gusmao Yeda Vieira Povoas 《Journal of Civil Engineering and Architecture》 2010年第10期66-70,共5页
One of the big problems of the urban centres of the cities in Brazil is the growth of the generation of the Civil Construction Waste (CCW). A disturbing concern for the public and private sectors is to find proper d... One of the big problems of the urban centres of the cities in Brazil is the growth of the generation of the Civil Construction Waste (CCW). A disturbing concern for the public and private sectors is to find proper disposal of Urban Solid Waste (USW) in large cities, since suitable dumping sites for this waste are increasingly scarce due to the spread and development of large urban centres. In general, recycling is currently one of the procedures most studied by researchers for disposal of such waste. In this context and aiming a new application for recycled CCW aggregates, the research characterized the physical properties of the material to use in fill compaction piles in soil improvement. The analysis shows results from laboratorial tests executed in CCW recycled samples, which came from works in different construction stages from Recife-PE, and natural aggregate, adopted as a reference. Characterization tests were performed with samples CCW recycled CLue aggregates and samples of natural fine aggregate (stone powder). The results of characterization tests showed similarities between the CCW samples tested and the reference samples, indicating that the civil construction wastes has potcntial use as material for consolidation piles in foundation works. 展开更多
关键词 Civil Construction Waste (CCW) recycled aggregate characterization tests.
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Dynamic behavior of micro-diaphragms and its characterized description 被引量:2
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作者 GE LiFeng 《Science China(Physics,Mechanics & Astronomy)》 SCIE EI CAS 2009年第9期1345-1356,共12页
Diaphragm structures with micron scale play a significant role in microtransducers and micro-nano devices,and the performance of these devices depends mainly on the dynamic behaviour of diaphragms.Micro-diaphragms are... Diaphragm structures with micron scale play a significant role in microtransducers and micro-nano devices,and the performance of these devices depends mainly on the dynamic behaviour of diaphragms.Micro-diaphragms are treated commonly as membranes and in some cases as plates or plates in tension(called TD plates for short),but they also show in many cases the behaviour of plates in tension and supported by air spring(called TDK plates for short).Therefore,it is necessary to perform systematic research on the dynamic behaviour of micro-diaphragms,and establish a characterized mathematical description.This paper focuses on the TDK plates since they possess universality,gives the corresponding basic equations,and then derives analytical solutions of TDK circular plates under clamped and simply supported boundary conditions.This paper also gives a 3D plot representation of characteristic curved surfaces,revealing the transition from the TDK and TD plate to the pure plate or pure membrane behaviour;and further uses the valueφto determine the property of diaphragms.Its two extreme cases,i.e.φ=0 andφ=∞,correspond to pure plate or pure membrane,respectively.Thus,membrane,plate and TD plate can be treated as special cases of TDK plate.In addition,this paper reveals that the presence of air-spring not only enhances the restoring force of diaphragm such that increases its natural frequencies,but also results in the resonance of a dynamic system consisting of diaphragm and air-spring.These analytical and computational results are significant for the understanding of the operation mechanism of capacitive microtransducers and their optimized design. 展开更多
关键词 micro-diaphragm membrane and plate TD plate and TDK plate dynamic behaviour characterized description
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Discovery of a Novel Ginseng Polysaccharide:Structure Characterization,in vitro Fermentability and Anti-oxidative Mechanism of Fermented Product via the Nrf2/HO-1 Pathway on Aβ-induced-PC 12 Cells
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作者 DONG Binbin HOU Zong +3 位作者 ZHENG Zhong XING Junpeng LIU Zhiqiang LIU Shu 《高等学校化学学报》 北大核心 2026年第1期173-189,共17页
In this study,a novel polysaccharide GPA-G 2-H was derived from ginseng.Furthermore,the coherent study of its structural characteristics,fermented characteristics in vitro,as well as antioxidant mechanism of fermented... In this study,a novel polysaccharide GPA-G 2-H was derived from ginseng.Furthermore,the coherent study of its structural characteristics,fermented characteristics in vitro,as well as antioxidant mechanism of fermented product FGPA-G 2-H on Aβ25-35-induced PC 12 cells were explored.The structure of GPA-G 2-H was determined by means of zeta potential analysis,FTIR,HPLC,XRD,GC-MS and NMR.The backbone of GPA-G 2-H was mainly composed of→4)-α-D-Glcp-(1→with branches substituted at O-3.Notably,GPA-G 2-H was degraded by intestinal microbiota in vitro with total sugar content and pH value decreasing,and short-chain fatty acids(SCFAs)increasing.Moreover,GPA-G 2-H significantly promoted the proliferation of Lactobacillus,Muribaculaceae and Weissella,thereby making positive alterations in intestinal microbiota composition.Additionally,FGPA-G 2-H activated the Nrf 2/HO-1 signaling pathway,enhanced HO-1,NQO 1,SOD and GSH-Px,while inhabited Keap 1,MDA and LDH,which alleviated Aβ-induced oxidative stress in PC 12 cells.These provide a solid theoretical basis for the further development of ginseng polysaccharides as functional food and antioxidant drugs. 展开更多
关键词 Ginseng polysaccharide Structural characterization Intestinal microbiota FERMENTABILITY Oxidative stress
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Effect of Development Stage on the Artemisinin Content and the Sequence Characterized Amplified Region (SCAR) Marker of High-Artemisinin Yielding Strains of Artemisia annua L.
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作者 Long Zhang He-Chun Ye Guo-Feng Li 《Journal of Integrative Plant Biology》 SCIE CAS CSCD 2006年第9期1054-1062,共9页
The effects of development states on the artemisinin content of clone S1 of Artemisia anuua L. grown in a greenhouse were investigated in the present study. The artemisinin content increased gradually during the phase... The effects of development states on the artemisinin content of clone S1 of Artemisia anuua L. grown in a greenhouse were investigated in the present study. The artemisinin content increased gradually during the phase of vegetative growth and reached its highest level at 8-9 mg/g dry weight (DW) when the S1 was 6 months old on a long day (LD) photoperiod. Treatment with 9-18 d of short day (SD) photoperiod resulted in the artemisinin content reaching and being maintained at a higher level (2.059-2.289 mg/g DW), twofold that of control plants and plants of S1 presented at the pro-flower budding and flower-budding stages. The artemisinin content varied in different parts of the plant. The artemisinin content of leaves was higher than that of florets and branches. The artemisinin content in middle leaves was higher than that of bottom leaves, and then top leaves. Different densities of capitate glands (the storage organ of artemisinin) located on the surface of leaves, florets, and branches explained the variations in artemisinin content in these parts of the plant. The correlation coefficient between artemisinin content and density of capitate glands on the surface of different organs was 0.987. The genetic marker for artemisinin content was screened using random amplified polymorphic DNA (RAPD) and sequence characterized amplified region (SCAR) techniques. The random primer OPAl5 (5'-TTCCGAACCC-3') could amplify a specific band of approximately 1 000 bp that was present in all high-artemisinin yielding strains, but absent in all low-yielding strains in three independent replications. This specific band was cloned and its sequence was analyzed. This RAPD marker was converted into a SCAR marker to obtain a more stable marker. 展开更多
关键词 Artemisia annua ARTEMISININ capitate gland random amplified polymorphic DNA (RAPD) sequence characterized amplified region (SCAR)
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THE PRINCESS AND THE PEA
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作者 Ruth Devlin 《空中英语教室(初级版.大家说英语)》 2026年第1期12-16,51,56,共7页
Small things can show a person's true character Find lt What was the weather like when the girl came to the castle door?nce upon a time,there was a prince.He wanted to marry a real princess.He traveled all around ... Small things can show a person's true character Find lt What was the weather like when the girl came to the castle door?nce upon a time,there was a prince.He wanted to marry a real princess.He traveled all around the world looking for one. 展开更多
关键词 WEATHER PRINCE PRINCESS true character
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Controllable phase-reconstruction strategy for LiFePO_(4)homogeneous regeneration:Reaction mechanism,characterization and prospect
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作者 Zihao Zeng Yi Chen +4 位作者 Chao Zhu Hai Lei Wei Sun Yue Yang Peng Ge 《Journal of Energy Chemistry》 2026年第1期553-571,I0013,共20页
The growing volume of end-of-life lithium-ion batteries(LIBs)represents both an urgent environmental challenge and a critical resource opportunity,especially for cathode materials.Among commercial cathodes,LiFePO4(LFP... The growing volume of end-of-life lithium-ion batteries(LIBs)represents both an urgent environmental challenge and a critical resource opportunity,especially for cathode materials.Among commercial cathodes,LiFePO4(LFP)dominates the market due to its favorable properties;thus,a substantial amount of LFP cathode materials is expected to retire in the near future.The conventional hydrometallurgical method suffers from high costs and serious pollution.Direct regeneration technologies,especially solid-state sintering,provide a more efficient and environmentally benign alternative by repairing cathode structures through high-temperature solid-phase reactions without extra chemical reagents.Traditional solid-state sintering faces challenges in processing spent LFP from diverse sources,struggling to achieve the homogenization of physical–chemical properties and electrochemical performance.To address the limitations above,phase homogenization with a lattice reconstruction strategy has been investigated,which can enable effective lattice reconstruction and microstructural homogenization,demonstrating robust adaptability to spent samples from variable sources.This review systematically summarizes the mechanisms,detailed steps,characterization techniques,and advances in pre-oxidation optimization(including ion-doping and coated carbon layer modification),as well as future research directions for sustainable LFP recycling.Given this,this review is expected to offer theoretical guidance for achieving homogeneous regeneration of LFP cathode. 展开更多
关键词 Spent LiFePO_(4) REGENERATION Phase-reconstruction Reaction mechanism CHARACTERIZATION
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Integrative identification of Aphelenchoides fragariae(Nematoda:Aphelenchoididae) parasitizing Fuchsia×hybrida in China
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作者 Qiuling Huang Yan Liao +6 位作者 Chunhui Huang Huan Peng Lingchiu Tsang Borong Lin Deliang Peng Jinling Liao Kan Zhuo 《Journal of Integrative Agriculture》 2026年第2期769-774,共6页
The strawberry crimp nematode(Aphelenchoides fragariae) is a serious pathogen of ornamental crops and a significant quarantine concern in approximately 50 countries and regions,including China.A nematode population be... The strawberry crimp nematode(Aphelenchoides fragariae) is a serious pathogen of ornamental crops and a significant quarantine concern in approximately 50 countries and regions,including China.A nematode population belonging to the genus Aphelenchoides was isolated from symptomatic leaves of fuchsia plants(Fuchsia×hybrida Hort.ex Sieb.& Voss.) in Chengdu,Sichuan Province,China.Morphological and morphometric characteristics were determined using light microscopy and scanning electron microscopy.Detailed examination revealed diagnostic features consistent with A.fragariae.Three ribosomal DNA(rDNA) regions,i.e.,partial small subunit(SSU) rRNA,D2-D3 expansion segments of the large subunit(LSU) rRNA,and the internal transcribed spacer(ITS),were amplified and sequenced.Bayesian phylogenetic analyses based on these sequences placed the isolate in a well-supported monophyletic clade with reference A.fragariae specimens,clearly separated from other Aphelenchoides species.Furthermore,host-suitability assays demonstrated that this nematode population not only infects and reproduces on Fuchsia×hybrida,but also on Fragaria ananassa and Pteris vittata,two known hosts of A.fragariae.Collectively,morphological,molecular,and host-range evidence confirm the identification of this nematode as A.fragariae.To our knowledge,this represents the first molecular and morphological confirmation of A.fragariae in China,and the first report of Fuchsia×hybrida as a natural host for this species. 展开更多
关键词 strawberry crimp nematode morphology molecular characterization host-suitability test fuchsia
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Effect of Tannin and Nanocellulose on Properties of Sustainable Cellulose Acetate Films
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作者 JoséMarcelo Faria de Queiroz Júnior Nathália Ramosde Melo +2 位作者 Ulisses Oliveira Costa Letícia Vitorazi Flavia Lega Braghiroli 《Journal of Renewable Materials》 2026年第2期144-170,共27页
The development of sustainable materials has encouraged the use of biopolymers as alternatives to synthetic polymers.Polymeric films have stood out for their high potential in environmentally sustainable applications.... The development of sustainable materials has encouraged the use of biopolymers as alternatives to synthetic polymers.Polymeric films have stood out for their high potential in environmentally sustainable applications.Conventional cellulose acetate(CA)-based films are attractive due to their biodegradability and film-forming ability.However,their functional performance often requires enhancement through the incorporation of additives.In this context,two bio-based additives were investigated:condensed tannin(0%,5%and 10%wt.),a natural polyphenol known for its antioxidant and antimicrobial properties,and nanocrystalline cellulose(CNC)(0%,0.5%and 1%wt.),which act as reinforcing agents to improve mechanical strength and barrier properties.The results showed that tannin generally enhanced mechanical strength and surface uniformity while imparting contact-based antimicrobial activity.CNC reduced water uptake and improved thermal stability,but when used alone,it tended to lower mechanical performance and increase surface roughness.The combination of CNC and tannin produced performance shifts that depended strongly on their relative concentrations,with no consistent synergistic effect across all properties.In certain balanced ratios,CNC benefited fromtannin’smatrix-stabilizing effect,leading to improved strength or reduced moisture absorption.Antimicrobial activity in acetic acid–based films was linked to residual acidity,whereas in acetonebased films,tannin alone was responsible for the antimicrobial effect by contact.These findings highlight that the physicochemical,mechanical,and functional performance of CA films(CAFs)is governed not only by additive type but also by the precise interplay between CNC and tannin,underscoring the need for formulation strategies tailored to the requirements of specific applications. 展开更多
关键词 Cellulose derivatives crystalline nanocellulose condensed tannin biobased films CHARACTERIZATION
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Research on characterization methods for track irregularities
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作者 Lifeng Xin Lei Xu +3 位作者 Jiaxin Zhang Menglin Pei Jianfeng Mao Dangxiong Wang 《Railway Engineering Science》 2026年第1期25-39,共15页
The characterization of track irregularities is crucial in railway dynamics,as track irregularities are the primary source of internal excitation in railway systems.In this paper,three mathematical models are proposed... The characterization of track irregularities is crucial in railway dynamics,as track irregularities are the primary source of internal excitation in railway systems.In this paper,three mathematical models are proposed to characterize the track irregularities under different circumstances.The first model is a novel explicit track spectrum function,which performs better in reflecting the inherent periodic components of track irregularities than the existing track spectra.On this foundation,the second model,a parameterized track spectrum random model,is proposed to represent the vast measured track irregularities from the probabilistic perspective.Finally,the third model,an imprecise track spectrum interval model based on a neighborhood uniform sampling Bootstrap method,is presented to identify the confidential interval of the track spectra when the track irregularity data are limited.Three examples are illustrated to demonstrate the feasibility of the three track irregularity models in characterizing the track irregularities in different conditions.This research can help capture the railway deformation status and optimize track maintenance strategies. 展开更多
关键词 RAILWAY Track irregularity Power spectral density CHARACTERIZATION Random model Imprecise track spectrum
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Coupled Effects of Single-Vacancy Defect Positions on the Mechanical Properties and Electronic Structure of Aluminum Crystals
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作者 Binchang Ma Xinhai Yu Gang Huang 《Computers, Materials & Continua》 2026年第1期332-352,共21页
Vacancy defects,as fundamental disruptions in metallic lattices,play an important role in shaping the mechanical and electronic properties of aluminum crystals.However,the influence of vacancy position under coupled t... Vacancy defects,as fundamental disruptions in metallic lattices,play an important role in shaping the mechanical and electronic properties of aluminum crystals.However,the influence of vacancy position under coupled thermomechanical fields remains insufficiently understood.In this study,transmission and scanning electron microscopy were employed to observe dislocation structures and grain boundary heterogeneities in processed aluminum alloys,suggesting stress concentrations and microstructural inhomogeneities associated with vacancy accumulation.To complement these observations,first-principles calculations and molecular dynamics simulations were conducted for seven single-vacancy configurations in face-centered cubic aluminum.The stress response,total energy,density of states(DOS),and differential charge density were examined under varying compressive strain(ε=0–0.1)and temperature(0–600 K).The results indicate that face-centered vacancies tend to reduce mechanical strength and perturb electronic states near the Fermi level,whereas corner and edge vacancies appear to have weaker effects.Elevated temperatures may partially restore electronic uniformity through thermal excitation.Overall,these findings suggest that vacancy position exerts a critical but position-dependent influence on coupled structure-property relationships,offering theoretical insights and preliminary experimental support for defect-engineered aluminum alloy design. 展开更多
关键词 Aluminum crystal vacancy defect microstructural characterization stress response electronic structure thermomechanical coupling
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A comprehensive review on oxygen vacancies modified catalysts:Synthesis,characterization,and crucial role in catalytic ozonation
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作者 Fengchen Wang Yujia Xiang +9 位作者 Yuqi Zhang Xin Zhou Jing Zhang Chuanshu He Heng Zhang Zhaokun Xiong Peng Zhou Hongyu Zhou Yang Liu Bo Lai 《Chinese Chemical Letters》 2026年第2期253-262,共10页
Among various advanced oxidation processes(AOPs),heterogeneous catalytic ozonation has garnered extensive attention in wastewater treatment owing to its broad pH range applicability and the elimination of the need for... Among various advanced oxidation processes(AOPs),heterogeneous catalytic ozonation has garnered extensive attention in wastewater treatment owing to its broad pH range applicability and the elimination of the need for additional energy input.Enhancing catalyst activity by introducing oxygen vacancies has been used extensively in heterogeneous catalytic ozonation.This paper reviews prevalent methods for the construction and characterization of oxygen vacancies.Based on a thorough examination of existing research,the role of oxygen vacancies is categorized according to their primary mechanisms of action in heterogeneous catalytic ozonation.For example,modulation of the catalyst electronic structure to enhance electron transfer;participation in the reaction as an active site to generate radicals and non-radicals;and exposure of more metal sites to enhance the reaction.Lastly,the paper delineates the limitations and future research directions concerning the role of oxygen vacancies in catalytic ozonation.This review addresses the gap in existing literature concerning the role of oxygen vacancies in catalytic ozone systems,establishes a comprehensive theoretical framework to aid in the design of efficient ozone catalysts,and delves into the functionality of oxygen vacancies in heterogeneous catalytic ozone reactions. 展开更多
关键词 Catalytic ozonation Bulk defects Surface defects Oxygen vacancies Degradation mechanism Synthesis and characterization
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In‑Operando X‑Ray Imaging for Sobering Examination of Aqueous Zinc Metal Batteries
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作者 Yuhang Dai Hongzhen He +4 位作者 Mengzheng Ouyang Jianuo Chen Jie Lin Haobo Dong Guanjie He 《Nano-Micro Letters》 2026年第3期330-337,共8页
Aqueous zinc metal batteries(AZMBs)face significant challenges in achieving reversibility and cycling stability,primarily due to hydrogen evolution reactions(HER)and zinc dendrite growth.In this study,by employing car... Aqueous zinc metal batteries(AZMBs)face significant challenges in achieving reversibility and cycling stability,primarily due to hydrogen evolution reactions(HER)and zinc dendrite growth.In this study,by employing carefully designed cells that approximate the structural characteristics of practical batteries,we revisit this widely held view through in-operando X-ray radiography to examine zinc dendrite formation and HER under nearpractical operating conditions.While conventional understanding emphasizes the severity of these processes,our findings suggest that zinc dendrites and HER are noticeably less pronounced in dense,real-operation configurations compared to modified cells,possibly due to a more uniform electric field and the suppression of triple-phase boundaries.This study indicates that other components,such as degradation at the cathode current collector interface and configuration mismatches within the full cell,may also represent important barriers to the practical application of AZMBs,particularly during the early stages of electrodeposition. 展开更多
关键词 Aqueous Zn metal batteries X-ray imaging In situ characterization Degradation mechanism
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Insights into the geranylgeranyl pyrophosphate synthase(GGPPS)gene family in Osmanthus fragrans and the role of OfGGPPS13 in the formation of floral color and aroma
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作者 Hanruo Qiu Qingyin Tian +4 位作者 Guimin Zeng Chenchen Xie Xiulian Yang Lianggui Wang Yuanzheng Yue 《Journal of Integrative Agriculture》 2026年第1期138-149,共12页
Osmanthus fragrans is most famous for its strong aroma,and different varieties have different degrees of fragrance and color.Fragrance and color are important factors affecting the ornamental quality of O.fragrans.Ter... Osmanthus fragrans is most famous for its strong aroma,and different varieties have different degrees of fragrance and color.Fragrance and color are important factors affecting the ornamental quality of O.fragrans.Terpenoids are important secondary metabolites in plants,with β-carotene(C40)being the major pigment substance and linalool(C10)being the key aromatic component in O.fragrans.The geranylgeranyl pyrophosphate synthase genes(GGPPSs)play important roles in secondary metabolism in plants.However,the functions of the GGPPS family in floral color and fragrance formation has rarely been reported in O.fragrans.In this study,24 Of GGPPS genes were identified and classified into two subfamilies.The Of GGPPSs showed tissue-specific expression and Of GGPPS13 had highest expression in flowers.The Of GGPPS13 protein was localized to chloroplasts.The transcriptome data of Of GGPPS13 was verified by q RT-PCR and the expression level in‘Wanyingui'with strong aroma was higher than that in‘Zhuangyuanhong'with deep color at different flower development stages.Transient overexpression of Of GGPPS13 in O.fragrans petals showed that Of GGPPS13 increased the β-carotene content,the main color substance of O.fragrans,but decreased the linalool content,the main volatile organic compound(VOC)in the floral aroma of O.fragrans.Of GGPPS13 was indicated as the critical gene related to terpenoid synthesis in the floral aroma and color formation in O.fragrans.Our findings provide gene resources on the GGPPS gene family for further revealing the molecular regulation mechanism of the floral color and aroma formation in O.fragrans. 展开更多
关键词 GGPPS gene family Osmanthus fragrans oral color and aroma formation functional characterization
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High-frequency emphasized neural network reconstruction method for in situ synchrotron radiation ultrafast computed tomography characterization
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作者 Jing-Wei Li Yu Xiao +3 位作者 Yong-Cun Li Xiao-Fang Hu Guo-Hao Du Feng Xu 《Nuclear Science and Techniques》 2026年第3期5-17,共13页
There is a contradiction between the evolution rate of materials and the time resolution of SR-CT characterization in the in situ synchrotron radiation computed tomography(SR-CT)characterization of ultrafast evolution... There is a contradiction between the evolution rate of materials and the time resolution of SR-CT characterization in the in situ synchrotron radiation computed tomography(SR-CT)characterization of ultrafast evolution process.The sampling strategy of the ultra-sparse angle is an effective method for improving time resolution.Accurate reconstruction under sparse sampling conditions has always been a bottleneck problem.In recent years,convolutional neural networks have shown outstanding advantages in sparse-angle CT reconstruction given the development of deep learning.However,existing ideas did not consider the expression of high-frequency details in neural networks,limiting their application in accurate SR-CT characterization.A novel high-frequency information-constrained deep learning network(HFIC-Net)is proposed in response to this problem.Additional high-frequency information constraints are added to improve the accuracy of the reconstruction results.Further,a series of numerical reconstruction experiments are conducted to verify this new method,and the results indicate that the reconstruction results of HFIC-Net method effectively improve reconstruction quality.This new method uses only eight-angle projections to achieve the reconstruction effect of the filtered backprojection method(FBP)method in 360 projections.The results of the HFIC-Net method demonstrate clear boundaries and accurate detailed structures,correcting the misinformation caused by using other methods.For quantitative evaluation,the SSIM used to evaluate image structure similarity is increased from 0.1951,0.9212,and 0.9308 for FBP,FBP-Conv,and DDC-Net,respectively,to 0.9620 for HFIC-Net.Finally,the results of actual SR-CT experimental data indicate that the new method can suppress artifacts and achieve accurate reconstruction,and it is suitable for the in situ SR-CT accurate characterization of ultxafast evolution process. 展开更多
关键词 Accurate SR-CT characterization CT reconstruction Sparse-angle CT reconstruction problem High-frequency information constrained Deep learning
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Hypervelocity impact response and protection for the track steels of rocket sled system via light-gas gun experiments
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作者 Siwei Zhao Yi Zeng +5 位作者 Xuewen Zhou Weixing Zhao Botao Xie Jianbin Jing Yan Chen Yilun Liu 《Defence Technology(防务技术)》 2026年第2期282-293,共12页
Hypervelocity rocket sled systems are critical for testing advanced military technologies,yet track damage at speeds exceeding Mach 5 remains a significant challenge for system reliability and performance.In this stud... Hypervelocity rocket sled systems are critical for testing advanced military technologies,yet track damage at speeds exceeding Mach 5 remains a significant challenge for system reliability and performance.In this study,we investigated the hypervelocity impact response and protection for highstrength U71 Mn or bainitic steel used in rocket sled tracks.Flyer plate impact experiments using a two-stage light-gas gun were conducted to study the hypervelocity collision response,followed by the microstructural characterization via optical microscope,scanning electron microscopy equipped with electron backscatter diffraction to reveal underlying damage mechanisms.Then,the calibrated thermalmechanical coupled finite element simulations using the Johnson-Cook constitutive model and MieGrüneisen equation of state were carried out.Results indicated that bainitic steel exhibits superior impact resistance with predominantly smooth scratch-dominated damage due to its higher ductility.In contrast,U71 Mn suffered significant material spallation and crack propagation arising from brittle fracture mechanisms.Zinc-rich epoxy primer coatings effectively mitigated stress concentration and temperature rise in the substrate at impacting velocities below 2.4 km/s,so as to suppress the microstructural damage such as adiabatic shear bands and dynamic recrystallization.However,coating protection diminished at ultra-high-speed impacts due to the coating failure.Dimensional analysis established quantitative relationships of the gouge damage size to projectile mass,impact velocity,and material yield strength.This study provides in-depth insights into damage mechanisms in hypervelocity rail systems,demonstrating that bainitic steel combined with protective coatings can significantly enhance impact resistance and system reliability,offering valuable guidance for the design and optimization of hypervelocity testing platforms. 展开更多
关键词 Hypervelocity rocket sled Gouge of the track steels Two-stage light-gas gun Microstructural characterization Coating protection
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