The relatively conserved 18S is often used in the phylogenetic analysis of microalgae. However, whether it can really help in barcoding microalgae needs to be evaluated. In this study the multiple approaches of coales...The relatively conserved 18S is often used in the phylogenetic analysis of microalgae. However, whether it can really help in barcoding microalgae needs to be evaluated. In this study the multiple approaches of coalescent, distance and character-based barcoding are first employed in C hlorella and Scenedesmus to test the efficiency of 18S sequences for barcoding green microalgae. We show that most Chlorella and Scenedesmus species, including the cryptic species, can be distinguished by 18S sequences with all coalescent General Mixed Yule-coalescent(GMYC), poisson tree process(PTP), and P ID, distance(ABGD) and character-based approaches. Both GMYC and PTP analyses produce more genetic groups. The P ID and ABGD analyses only cluster some species. All species(apart from a few of lineages) can be separated in character-based barcoding analysis with more than three character attributes. In comparison with previous barcoding results with r bcL, tufA, ITS and 16 S, 18S produces good resolution in identifying Chlorella and Scenedesmus. Our results reveal that 18S is highly efficient in identifying taxa of green microalgae at species level, based on a combination of multiple barcoding approaches. Combining 18S with other gene markers may be useful in barcoding microalgae.展开更多
The Department of Education(DepEd)remains unwavering in its commitment to guiding and nurturing Filipinos who hold a deep love for their country and actively participate in nation-building.It steadfastly pursues a lea...The Department of Education(DepEd)remains unwavering in its commitment to guiding and nurturing Filipinos who hold a deep love for their country and actively participate in nation-building.It steadfastly pursues a learner-centered approach,instilling values that inspire youth to be God-fearing,humane,environmentally conscious,and patriotic.In line with its mission,DepEd CALABARZON aligns with this vision and emphasizes the importance of values education in the Philippine Curriculum.Recognizing the significance of values,DepEd consistently integrates core values-MakaDiyos,Makatao,Makakalikasan,and Makabansa-into its educational framework.These values are assessed quarterly alongside academic achievements,shaping learners into well-rounded individuals.Edukasyon sa Pagpapakatao and the Homeroom Guidance Program further foster commendable values and attitudes,motivating students to excel in both education and employment.However,the current assessment and recognition system fails to emphasize the importance of value formation,leading students to undervalue these essential qualities as they complete basic education.A cultural shift is necessary to promote positive values that foster a highly innovative and trust-based society.DepEd CALABARZON conducts a qualitative-quantitative study to identify factors contributing to learners’values,characters,and attitudes and the challenges they face in evolving these traits.The study aims to propose an innovative character education program rooted in DepEd’s core values,Edukasyon sa Pagpapakatao,and Homeroom Guidance competencies.This program seeks to recognize excellence in shaping fully developed CALABARZON learners,thereby strengthening DepEd’s core values and producing notable graduates in the future.展开更多
基金Supported by the China Post-doctoral Science Foundation(Nos.2014M561661,2015T80558)the Natural Science Foundation of Jiangsu Province(No.BK20150680)+1 种基金the National Natural Science Foundation of China(No.31600294)the Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization and Fundamental Research Funds for the Central Universities of Ministry of Education of China(No.Y0201600141)
文摘The relatively conserved 18S is often used in the phylogenetic analysis of microalgae. However, whether it can really help in barcoding microalgae needs to be evaluated. In this study the multiple approaches of coalescent, distance and character-based barcoding are first employed in C hlorella and Scenedesmus to test the efficiency of 18S sequences for barcoding green microalgae. We show that most Chlorella and Scenedesmus species, including the cryptic species, can be distinguished by 18S sequences with all coalescent General Mixed Yule-coalescent(GMYC), poisson tree process(PTP), and P ID, distance(ABGD) and character-based approaches. Both GMYC and PTP analyses produce more genetic groups. The P ID and ABGD analyses only cluster some species. All species(apart from a few of lineages) can be separated in character-based barcoding analysis with more than three character attributes. In comparison with previous barcoding results with r bcL, tufA, ITS and 16 S, 18S produces good resolution in identifying Chlorella and Scenedesmus. Our results reveal that 18S is highly efficient in identifying taxa of green microalgae at species level, based on a combination of multiple barcoding approaches. Combining 18S with other gene markers may be useful in barcoding microalgae.
基金funded by the Basic Education Research Fund(BERF)。
文摘The Department of Education(DepEd)remains unwavering in its commitment to guiding and nurturing Filipinos who hold a deep love for their country and actively participate in nation-building.It steadfastly pursues a learner-centered approach,instilling values that inspire youth to be God-fearing,humane,environmentally conscious,and patriotic.In line with its mission,DepEd CALABARZON aligns with this vision and emphasizes the importance of values education in the Philippine Curriculum.Recognizing the significance of values,DepEd consistently integrates core values-MakaDiyos,Makatao,Makakalikasan,and Makabansa-into its educational framework.These values are assessed quarterly alongside academic achievements,shaping learners into well-rounded individuals.Edukasyon sa Pagpapakatao and the Homeroom Guidance Program further foster commendable values and attitudes,motivating students to excel in both education and employment.However,the current assessment and recognition system fails to emphasize the importance of value formation,leading students to undervalue these essential qualities as they complete basic education.A cultural shift is necessary to promote positive values that foster a highly innovative and trust-based society.DepEd CALABARZON conducts a qualitative-quantitative study to identify factors contributing to learners’values,characters,and attitudes and the challenges they face in evolving these traits.The study aims to propose an innovative character education program rooted in DepEd’s core values,Edukasyon sa Pagpapakatao,and Homeroom Guidance competencies.This program seeks to recognize excellence in shaping fully developed CALABARZON learners,thereby strengthening DepEd’s core values and producing notable graduates in the future.
