Healthcare waste has now been increasingly studied in terms of the risks or dangers that can cause the environment and human health. Waste generated in clinical analysis laboratories (CALs) deserves attention, because...Healthcare waste has now been increasingly studied in terms of the risks or dangers that can cause the environment and human health. Waste generated in clinical analysis laboratories (CALs) deserves attention, because, due to the advent of the concept of emergent pollution, it is doubtful if the materials or reagents are disposed in the sewage by CALs, which are currently considered non-contaminated or with low risk potential, under current legislation, may actually impact the environment with actions not yet understood. This study was experimental and conducted at the Environmental Laboratory of the University of the Region of Joinville. It was used Euglena gracilis (primary trophic level) algae exposed to effluents from five sectors of a CAL: Biochemistry, Hematology, Viral Load, Tuberculosis and Immunochemistry. Samples were collected from the siphons attached to the wash sinks of the CAL materials. To verify changes in algae that denote environmental danger, behavioral changes were analyzed via NGTOX, and chlorophyll concentration was calculated by chlorophyll extraction according to Mendel’s method. Viral Load (VL) and Hematology (HT) sectors were the ones that most affected algae (Tukey test). In both sectors, there was inhibition of algae mobility and gravitaxy: in VL, due to the presence of chaotropic agents that denature organic structures;and in HT, due to the change in membrane permeability attributed to methylene blue. Also in HT, there was a search for algae adaptation by increasing the rise to the surface in order to overcome the lower luminosity due to the coloration of the environment, which also affects photosynthesis. Regarding the concentration of a-chlorophyll, the VL and HT were the most affected as well, being the first one the one that had more concentration reduction because of the presence of chaotropic agents. Considering new parameters evaluated, the discarded compounds need to be better evaluated for risk, as they affect algal photosynthesis. Procedures for removal of these compounds should be considered.展开更多
Innovative advancements in the development of high-performance,eco-friendly adhesives are critical for meeting the demands of diverse applications in various industries.This study reports a significant leap in adhesiv...Innovative advancements in the development of high-performance,eco-friendly adhesives are critical for meeting the demands of diverse applications in various industries.This study reports a significant leap in adhesive technology by enhancing the interfacial toughness and versatility of polyvinyl alcohol(PVA)through complexation with 1-nm Keggin-type polyoxotungstate clusters(POTs)carrying specific negative charges.The POT-PVA nanocomposites exhibit superior adhesion to hydrophilic surfaces,attributed to their high crosslinking densities and exceptional fracture energies surpassing 6.23 kJ·m^(-2).These adhesives,endowed with high flexibility and a wealth of surface hydroxyl groups,are uniquely suited for application on a wide array of substrates including glass,steel,aluminum,and beyond,demonstrating their broad applicability.Specifically,the reduction in PVA crystallinity due to the chaotropic effect of POTs,which significantly enhances polymer chain dynamics.This enhancement confers robust adhesive properties at extreme temperatures,from the cryogenic−196℃ to the high-temperature threshold of 100℃.By capitalizing on the chaotropic effects of charged POTs,the study achieves a notable enhancement in the adhesive capabilities of the POT-PVA nanocomposites,paving the way for the development of for eco-friendly and cost effective adhesives engineered to withstand extreme conditions.展开更多
The kosmotropicity of cations and anions in ionic liquids has a strong influence on the enzyme catalytic efficiency in aqueous environments. The kosmotropic anion CF3COO^- seemed to activate the protease, and the chao...The kosmotropicity of cations and anions in ionic liquids has a strong influence on the enzyme catalytic efficiency in aqueous environments. The kosmotropic anion CF3COO^- seemed to activate the protease, and the chaotropic anions tended to destabilize the enzyme.展开更多
文摘Healthcare waste has now been increasingly studied in terms of the risks or dangers that can cause the environment and human health. Waste generated in clinical analysis laboratories (CALs) deserves attention, because, due to the advent of the concept of emergent pollution, it is doubtful if the materials or reagents are disposed in the sewage by CALs, which are currently considered non-contaminated or with low risk potential, under current legislation, may actually impact the environment with actions not yet understood. This study was experimental and conducted at the Environmental Laboratory of the University of the Region of Joinville. It was used Euglena gracilis (primary trophic level) algae exposed to effluents from five sectors of a CAL: Biochemistry, Hematology, Viral Load, Tuberculosis and Immunochemistry. Samples were collected from the siphons attached to the wash sinks of the CAL materials. To verify changes in algae that denote environmental danger, behavioral changes were analyzed via NGTOX, and chlorophyll concentration was calculated by chlorophyll extraction according to Mendel’s method. Viral Load (VL) and Hematology (HT) sectors were the ones that most affected algae (Tukey test). In both sectors, there was inhibition of algae mobility and gravitaxy: in VL, due to the presence of chaotropic agents that denature organic structures;and in HT, due to the change in membrane permeability attributed to methylene blue. Also in HT, there was a search for algae adaptation by increasing the rise to the surface in order to overcome the lower luminosity due to the coloration of the environment, which also affects photosynthesis. Regarding the concentration of a-chlorophyll, the VL and HT were the most affected as well, being the first one the one that had more concentration reduction because of the presence of chaotropic agents. Considering new parameters evaluated, the discarded compounds need to be better evaluated for risk, as they affect algal photosynthesis. Procedures for removal of these compounds should be considered.
基金This study was financially supported by the National Natural Science Foundation of China(No.22101086)Guangdong Basic and Applied Basic Research Foundation(Nos.2023A1515140030 and 2024A1515030212).
文摘Innovative advancements in the development of high-performance,eco-friendly adhesives are critical for meeting the demands of diverse applications in various industries.This study reports a significant leap in adhesive technology by enhancing the interfacial toughness and versatility of polyvinyl alcohol(PVA)through complexation with 1-nm Keggin-type polyoxotungstate clusters(POTs)carrying specific negative charges.The POT-PVA nanocomposites exhibit superior adhesion to hydrophilic surfaces,attributed to their high crosslinking densities and exceptional fracture energies surpassing 6.23 kJ·m^(-2).These adhesives,endowed with high flexibility and a wealth of surface hydroxyl groups,are uniquely suited for application on a wide array of substrates including glass,steel,aluminum,and beyond,demonstrating their broad applicability.Specifically,the reduction in PVA crystallinity due to the chaotropic effect of POTs,which significantly enhances polymer chain dynamics.This enhancement confers robust adhesive properties at extreme temperatures,from the cryogenic−196℃ to the high-temperature threshold of 100℃.By capitalizing on the chaotropic effects of charged POTs,the study achieves a notable enhancement in the adhesive capabilities of the POT-PVA nanocomposites,paving the way for the development of for eco-friendly and cost effective adhesives engineered to withstand extreme conditions.
基金Project supported by the NIH-EARDA grant （No. 5G11HD32861-08）-and-the National Science Foundation MAGEC-STEM Summer Research Program （2005）.
文摘The kosmotropicity of cations and anions in ionic liquids has a strong influence on the enzyme catalytic efficiency in aqueous environments. The kosmotropic anion CF3COO^- seemed to activate the protease, and the chaotropic anions tended to destabilize the enzyme.
