AIM: To validate accuracy of a novel chair-side test for matrix metalloproteinase(MMP)-8 as compared to enzyme-linked immunosorbent assay(ELISA) in Periodontal health and disease.METHODS: Gingival crevicular fluid was...AIM: To validate accuracy of a novel chair-side test for matrix metalloproteinase(MMP)-8 as compared to enzyme-linked immunosorbent assay(ELISA) in Periodontal health and disease.METHODS: Gingival crevicular fluid was collected from 150 subjects, Group 1(healthy)- 50 subjects, Group 2(gingivitis)- 50 subjects and Group 3(chronic periodontitis)- 50 subjects. A chair-side test strip was indigenously prepared using polyclonal antibodies(principle of immunochromatography) to detect the MMP-8 levels. The detection accuracy(sensitivity and specificity) of the MMP-8 levels by chair-side test kit were compared with ELISA at baseline and 3 mo after scaling and root planing among the study population.RESULTS: The novel chair side test detected MMP-8 levels in accordance with ELISA which at baseline were higher in Group 2 and Group 3 as compared to controls(P < 0.05), and these enzyme levels decreased after therapy(P < 0.05). The chair-side test could differentiate healthy, gingivitis and periodontitis. The detection accuracy of the chair-side test strip were on par with ELISA(sensitivity 92.9% and specificity of 100%) which were statistically significant(P < 0.05). A desire to arouse interest about periodontal health and maintenance in the Indian population provided a strong rationale for us to develop our chair-side test strips to suit our economy. Moreover, this was the first ever effort to develop and validate a chair-side test strip to detect MMP-8 levels in the Indian population. This test can be used on a large scale in private dental practice for the early detection of disease, tapping the sites at risk for disease, alongside helps in patient education and motivation for maintenance.CONCLUSION: This study shows that the novel chair side test kit detects MMP-8 levels a biomarker of periodontal disease progression accurately making it a good chair side diagnostic tool. Further, it is cost effective and time saving which can make it applicable in private dental practice on a large scale for the early detection of periodontal disease.展开更多
Introduction: Several techniques have been described by which a pair of dentures may be replicated. Case Report: The method described in this paper used materials readily available in a private practice to generate a ...Introduction: Several techniques have been described by which a pair of dentures may be replicated. Case Report: The method described in this paper used materials readily available in a private practice to generate a pair of duplicate dentures for a 74-year-old Black male, which captured all of the features of the patients’ existing pair. These were modified chair-side and used to capture vital information on fit, occlusion and aesthetics, forming a template which was transferred to the laboratory and used to generate new dentures via the traditional laboratory process. The same template was also used to generate a pair of “AvaDent Digital Dentures” by digital scanning and production of a digital denture template for try and then fabrication of the digital denture. Conclusion: The results of this pilot study show that this simple chair-side method of fabricating duplicate dentures can be incorporated successfully into the AvaDent Digital Denture fabrication process to generate a pair of dentures with comparability if not superior fit and aesthetics to a pair fabricated from the same template via the traditional laboratory process.展开更多
目的评价椅旁全瓷局部瓷贴面在年轻恒前牙简单冠折中的修复效果。方法选取中山市人民医院口腔分院儿童口腔科就诊的46例52颗外伤冠折年轻恒前牙为研究对象,为牙釉质折断或牙釉质和牙本质折断,未露髓,随机分为椅旁局部瓷贴面组和树脂直...目的评价椅旁全瓷局部瓷贴面在年轻恒前牙简单冠折中的修复效果。方法选取中山市人民医院口腔分院儿童口腔科就诊的46例52颗外伤冠折年轻恒前牙为研究对象,为牙釉质折断或牙釉质和牙本质折断,未露髓,随机分为椅旁局部瓷贴面组和树脂直接修复组,按改良U S P H S评价标准于修复后即刻、1年及3年对修复体边缘密合度、解剖形态、表面光滑度、色彩匹配度及固位情况进行评价;在修复后3年进行患者满意度评价。结果椅旁局部瓷贴面组各评价指标随访期间基本达到良好等级。树脂直接修复组有24例(85.7%)在解剖形态上认为良好,边缘密合性评为良好的由即刻完成时的28例(100%)降至随访3年时的20例(76.9%),色彩匹配方面评为良好的由即刻的22例(78.6%)降至随访3年时的18例(69.2%),随访期间,有6例(23.1%)树脂修复体出现脱落而重新修复。结论椅旁局部瓷贴面在年轻恒前牙简单冠折中的3年临床修复效果满意,在边缘密合度、解剖形态、表面光滑度、色彩匹配度及固位情况等方面的表现均优于树脂修复。展开更多
文摘AIM: To validate accuracy of a novel chair-side test for matrix metalloproteinase(MMP)-8 as compared to enzyme-linked immunosorbent assay(ELISA) in Periodontal health and disease.METHODS: Gingival crevicular fluid was collected from 150 subjects, Group 1(healthy)- 50 subjects, Group 2(gingivitis)- 50 subjects and Group 3(chronic periodontitis)- 50 subjects. A chair-side test strip was indigenously prepared using polyclonal antibodies(principle of immunochromatography) to detect the MMP-8 levels. The detection accuracy(sensitivity and specificity) of the MMP-8 levels by chair-side test kit were compared with ELISA at baseline and 3 mo after scaling and root planing among the study population.RESULTS: The novel chair side test detected MMP-8 levels in accordance with ELISA which at baseline were higher in Group 2 and Group 3 as compared to controls(P < 0.05), and these enzyme levels decreased after therapy(P < 0.05). The chair-side test could differentiate healthy, gingivitis and periodontitis. The detection accuracy of the chair-side test strip were on par with ELISA(sensitivity 92.9% and specificity of 100%) which were statistically significant(P < 0.05). A desire to arouse interest about periodontal health and maintenance in the Indian population provided a strong rationale for us to develop our chair-side test strips to suit our economy. Moreover, this was the first ever effort to develop and validate a chair-side test strip to detect MMP-8 levels in the Indian population. This test can be used on a large scale in private dental practice for the early detection of disease, tapping the sites at risk for disease, alongside helps in patient education and motivation for maintenance.CONCLUSION: This study shows that the novel chair side test kit detects MMP-8 levels a biomarker of periodontal disease progression accurately making it a good chair side diagnostic tool. Further, it is cost effective and time saving which can make it applicable in private dental practice on a large scale for the early detection of periodontal disease.
文摘Introduction: Several techniques have been described by which a pair of dentures may be replicated. Case Report: The method described in this paper used materials readily available in a private practice to generate a pair of duplicate dentures for a 74-year-old Black male, which captured all of the features of the patients’ existing pair. These were modified chair-side and used to capture vital information on fit, occlusion and aesthetics, forming a template which was transferred to the laboratory and used to generate new dentures via the traditional laboratory process. The same template was also used to generate a pair of “AvaDent Digital Dentures” by digital scanning and production of a digital denture template for try and then fabrication of the digital denture. Conclusion: The results of this pilot study show that this simple chair-side method of fabricating duplicate dentures can be incorporated successfully into the AvaDent Digital Denture fabrication process to generate a pair of dentures with comparability if not superior fit and aesthetics to a pair fabricated from the same template via the traditional laboratory process.
文摘目的评价椅旁全瓷局部瓷贴面在年轻恒前牙简单冠折中的修复效果。方法选取中山市人民医院口腔分院儿童口腔科就诊的46例52颗外伤冠折年轻恒前牙为研究对象,为牙釉质折断或牙釉质和牙本质折断,未露髓,随机分为椅旁局部瓷贴面组和树脂直接修复组,按改良U S P H S评价标准于修复后即刻、1年及3年对修复体边缘密合度、解剖形态、表面光滑度、色彩匹配度及固位情况进行评价;在修复后3年进行患者满意度评价。结果椅旁局部瓷贴面组各评价指标随访期间基本达到良好等级。树脂直接修复组有24例(85.7%)在解剖形态上认为良好,边缘密合性评为良好的由即刻完成时的28例(100%)降至随访3年时的20例(76.9%),色彩匹配方面评为良好的由即刻的22例(78.6%)降至随访3年时的18例(69.2%),随访期间,有6例(23.1%)树脂修复体出现脱落而重新修复。结论椅旁局部瓷贴面在年轻恒前牙简单冠折中的3年临床修复效果满意,在边缘密合度、解剖形态、表面光滑度、色彩匹配度及固位情况等方面的表现均优于树脂修复。
