While most mammals show birth hour peaks at times of the 24-h cycle when they are less active,there are exceptions to this general pattern.Such exceptions have been little explored,but may clarify evolutionary reasons...While most mammals show birth hour peaks at times of the 24-h cycle when they are less active,there are exceptions to this general pattern.Such exceptions have been little explored,but may clarify evolutionary reasons for the diel timing of births.We investigated intraspecific variation in birth hour in wild blue monkeys Cercopithecus mitis stuhlmanni,a diurnal primate,to identify factors that differentiated daytime versus nighttime births.Behavioral and life history data from 14 groups over 14 years revealed that 4%of 484 births occurred during the day.Probability of daytime birth varied with mother’s age,peaking at 15.7 years.Births whose annual timing deviated most from the population’s peak birth months were 5 times more likely to occur during daytime than those that deviated less.There was no evidence that mother’s rank or infant sex influenced birth hour,and mixed evidence that daytime births were more probable in larger groups.Survivorship did not differ significantly for infants born during the day versus night.Prime-aged mothers may be able to handle the consequences of an unusual birth hour more successfully than mothers with less experience or those weakened by age.Daytime birth may be more advantageous in the off-season because nights are colder at that time of year.These findings are consistent with hypotheses relating birth hour to the risk of losing social protection in group-living animals,but are not consistent with those emphasizing risk of conspecific harassment.Patterns of within-species variation can help in evaluating evolutionary hypotheses for non-random birth hour.展开更多
OBJECTIVE: To evaluate the effectiveness of small interfering RNA (siRNA) on inhibiting severe acute respiratory syndrome (SARS)-associated coronavirus replication, and to lay bases for the future clinical application...OBJECTIVE: To evaluate the effectiveness of small interfering RNA (siRNA) on inhibiting severe acute respiratory syndrome (SARS)-associated coronavirus replication, and to lay bases for the future clinical application of siRNA for the treatment of viral infectious diseases. METHODS: Vero-E6 cells was transfected with siRNA before SARS virus infection, and the effectiveness of siRNA interference was evaluated by observing the cytopathic effect (CPE) on Vero-E6 cells. RESULTS: Five pairs of siRNA showed ability to reduce CPE dose dependently, and two of them had the best effect. CONCLUSION: siRNA may be effective in inhibiting SARS-associated coronavirus replication.展开更多
基金the U.S.National Science Foundation(SBE 9523623,BCS 9808273,DGE 0333415,BCS 0554747,DGE 0966166,BCS 1028471),Ford,Leakey,Wenner-Gren and H.F.Guggenheim Foundations,American Association for the Advancement of Science-Women's International Scientific Cooperation Project,and Columbia University,all to M.C.
文摘While most mammals show birth hour peaks at times of the 24-h cycle when they are less active,there are exceptions to this general pattern.Such exceptions have been little explored,but may clarify evolutionary reasons for the diel timing of births.We investigated intraspecific variation in birth hour in wild blue monkeys Cercopithecus mitis stuhlmanni,a diurnal primate,to identify factors that differentiated daytime versus nighttime births.Behavioral and life history data from 14 groups over 14 years revealed that 4%of 484 births occurred during the day.Probability of daytime birth varied with mother’s age,peaking at 15.7 years.Births whose annual timing deviated most from the population’s peak birth months were 5 times more likely to occur during daytime than those that deviated less.There was no evidence that mother’s rank or infant sex influenced birth hour,and mixed evidence that daytime births were more probable in larger groups.Survivorship did not differ significantly for infants born during the day versus night.Prime-aged mothers may be able to handle the consequences of an unusual birth hour more successfully than mothers with less experience or those weakened by age.Daytime birth may be more advantageous in the off-season because nights are colder at that time of year.These findings are consistent with hypotheses relating birth hour to the risk of losing social protection in group-living animals,but are not consistent with those emphasizing risk of conspecific harassment.Patterns of within-species variation can help in evaluating evolutionary hypotheses for non-random birth hour.
文摘OBJECTIVE: To evaluate the effectiveness of small interfering RNA (siRNA) on inhibiting severe acute respiratory syndrome (SARS)-associated coronavirus replication, and to lay bases for the future clinical application of siRNA for the treatment of viral infectious diseases. METHODS: Vero-E6 cells was transfected with siRNA before SARS virus infection, and the effectiveness of siRNA interference was evaluated by observing the cytopathic effect (CPE) on Vero-E6 cells. RESULTS: Five pairs of siRNA showed ability to reduce CPE dose dependently, and two of them had the best effect. CONCLUSION: siRNA may be effective in inhibiting SARS-associated coronavirus replication.
