Objective:To search for the most active antimicrobial and antioxidant sub-fractions related to traditional use of Ziziphus oxyphylla(Z.oxyphylla)and Cedrela serrata(C.serrata)in Pakistan against infectious and liver d...Objective:To search for the most active antimicrobial and antioxidant sub-fractions related to traditional use of Ziziphus oxyphylla(Z.oxyphylla)and Cedrela serrata(C.serrata)in Pakistan against infectious and liver diseases.Methods:Factions of different polarity were tested in vitro for their antiprotozoal,antimalarial,antibacterial and antifungal activity against different pathogens.Cytotoxicity on MRC-5 cell lines(human lung fibroblasts)as well as,in vitro radical scavenging activity was evaluated using the 1,1-diphenyl-2-picrylhydrazyl radical assay.Results:The highest antiprotozoal activity was observed for the CHCl_(3)fractions of Z.oxyphylla roots and leaves,and C.serrata bark.The CHCl_(3)and Et OAc fractions of Z.oxyphylla roots,the CHCl_(3)fraction of Z.oxyphylla leaves,the Et OAc and the residual Me OH:H_(2)O fraction of C.serrata bark showed antibacterial activity against Staphylococcus aureus.The same residual Me OH:H_(2)O fraction of C.serrata bark was active against Candida albicans.The highest antioxidant activity was observed for the more hydrophilic Et OAc fractions of Z.oxyphylla leaves,C.serrata bark and leaves,and the residual Me OH:H_(2)O fraction of C.serrata bark.Conclusions:This study supports at least in part the traditional uses of these plants for antimicrobial purposes and against liver diseases.展开更多
The association between the growth and water-use efficiency in three populations of C. odorata in Veracruz Mexico （La Antigua, Misantla and Catemaco） from two agroforestry system sites was studied, one in associatio...The association between the growth and water-use efficiency in three populations of C. odorata in Veracruz Mexico （La Antigua, Misantla and Catemaco） from two agroforestry system sites was studied, one in association with corn and another with pipiain. TRP （transpiration）, gs （stomatal conductivity）, CO2A （CO2 assimilation）, WUE （water use efficiency）, height, coverage and stem diameter were measured. The populafon of La Antigua associated with pipian presented the highest WUE （7.13 μmol CO2 m2-sl/mmol H20 m2.sl）, while the population of Catemaco reached high values （10 μmol CO2 m2 sl/mmol H20 m2-s1） in the site associated with corn, the populations established with corn presented a great growth in height and stem diameter. Based on the physiological responses and growth of the studied populations, the towns of La Antigua and Catemaco may be recommended for the establishment of commercial plantations.展开更多
A study was conducted to examine the germination requirements of Cedrela odorata, Guaiacum sanctum and Calycophyllum candidissimum seeds, and the effect of light intensity on survival and growth of C. odorata and G. s...A study was conducted to examine the germination requirements of Cedrela odorata, Guaiacum sanctum and Calycophyllum candidissimum seeds, and the effect of light intensity on survival and growth of C. odorata and G. sanctum seedlings planted on open, partially-open and beneath the canopy of a dry forest in Nicaragua. The results show that germination of C. candidissimum seeds was signifi- cantly higher in light than darkness at constant temperatures ranging from 20-35℃. Both C. odorata and G. sanctum seeds germinated in light and darkness to a varying extent, and the optimal germination temperature was around 20-25℃. Alternating temperature regimes did not improve germination. Survival of G. sanctum seedlings was 70% beneath the canopy and 80% in the open, and its relative growth rate in collar diameter was 1.88 mm/month in the open and 1.42 mm/month in the understory. Survival was generally poor for C. odorata, particularly in the understory. We concluded that light is an absolute requirement for the germination of C. candidissimum seeds while germination of C. odorata and G. sanctum seeds are more sensitive to temperature. Owing to its high survival rate on open site, G. sanctum could serve as a candidate species for reforestation of degraded sites.展开更多
Meliaceae plants are distinguished by the attack of the shootborer Hypsipyla grandella and also for the occurrence of limonoids, alkaloids and phenolic compounds. Such compounds extracted from leaves of Meliaceae spec...Meliaceae plants are distinguished by the attack of the shootborer Hypsipyla grandella and also for the occurrence of limonoids, alkaloids and phenolic compounds. Such compounds extracted from leaves of Meliaceae species Cedrela odorata L., Swietenia macrophylla King, Khaya senegalensis, Toona ciliata, and C. odorata grafted onto T. ciliata plants, were tested on C. odorata leaf disks to determine their effects on survival and performance of H. grandella larvae. Larval survival was assessed 2, 10 and 25 days after starting the bioassays. Leaf consumption and weight gain per larva, days to pupa and to adult stages, pupal weight and length, and moth wing appearance were assessed for larval performance. The three compunds from the four Meliaceae species and the grafted combination affected (P ≤ 0.02) larval leaf consumption, weight gain, time to pupa and to adult stages, wing development and larval survival of H. grandella. Pupa weight (P = 0.78, F = 0.72, d.f. = 18, 160) and length (P = 0.48, F = 0.98, d.f. = 18, 160) were similar regardless of the coumpound used. Limonoid reduced larval survival on the three dates of evaluation. Alkaloids decreased leaf consumption, weight gain of larvae and time needed to reach pupa and adult stages. Alkaloids from T. ciliata and phenols from C. odorata were the best coumpounds to reduce leaf consumption and weight gain. Alkaloids from the grafted plants caused 20% of H. grandella adults to form abnormal wings.展开更多
A study was conducted to examine the germination requirements of Cedrela odorata, Guaiacum sanctum and Calycophyllum candidissimum seeds, and the effect of light intensity on survival and growth of C.odorata and G.san...A study was conducted to examine the germination requirements of Cedrela odorata, Guaiacum sanctum and Calycophyllum candidissimum seeds, and the effect of light intensity on survival and growth of C.odorata and G.sanctum seedlings planted on open, partially-open and beneath the canopy of a dry forest in Nicaragua.The results show that germination of C.candidissimum seeds was significantly higher in light than darkness at constant temperatures ranging from 20-35oC.Both C.odorata and G.sanctum seeds germinated in light and darkness to a varying extent, and the optimal germination temperature was around 20-25oC.Alternating temperature regimes did not improve germination.Survival of G.sanctum seedlings was 70% beneath the canopy and 80% in the open, and its relative growth rate in collar diameter was 1.88 mm/month in the open and 1.42 mm/month in the understory.Survival was generally poor for C.odorata, particularly in the understory.We concluded that light is an absolute requirement for the germination of C.candidissimum seeds while germination of C.odorata and G.sanctum seeds are more sensitive to temperature.Owing to its high survival rate on open site, G.sanctum could serve as a candidate species for reforestation of degraded sites.展开更多
Water availability is a limiting factor for the establishment and development of forest species.To understand the appropriate conditions for the initial post-transplanting phase,it is necessary to understand the speci...Water availability is a limiting factor for the establishment and development of forest species.To understand the appropriate conditions for the initial post-transplanting phase,it is necessary to understand the specific morphophysiological characteristics of the species,such as the leaf water potential and the efficiency of photosystemⅡ.We aimed to identify the influence of different water regimes on the morphophysiological aspects of young plants of two forest species(Cedrela.fissilis Vellozo and Eucalyptus saligna Sm.).Two greenhouse experiments were conducted for 28 days;one for each species.The design was completely randomized,and the treatments consisted of six different water regimes.Leaf water potential(Ψw)and chlorophyll a fluorescence were evaluated every 7 days.At the end of the experiment,morphological attributes(height,collection diameter,root volume,and dry matter)were measured and histological blades were made.The water demand of E.saligna was higher than that of C.fissilis and required greater replacement within a shorter period.The rehydration fromΨw=-2 Mpa allowed for a fast recovery of the young C.fissilis plants(Ψw=-1.5,Fv/Fm=0.796),which indicated good physiological plasticity of this species when submitted to water stress at a level that is not severe.The total dry matter allocation was different among species.Seedlings of E.saligna presented the best responses when submitted to a continuous water supply regime,while C.fissilis seedlings presented the best response under intermittent irrigation conditions.展开更多
Aims Non-native invasive plants can alter soil chemistry through litter production and decomposition to facilitate their invasion.However,the important roles of these underlying processes in plant invasion remain poor...Aims Non-native invasive plants can alter soil chemistry through litter production and decomposition to facilitate their invasion.However,the important roles of these underlying processes in plant invasion remain poorly understood,particularly in tropical forest ecosys-tems.Here,we compared litter production,quality and decompos-ition of two invasive species(Broussonetia papyrifera and Cedrela odorata)and two co-occurring native species(Celtis mildbraedii and Funtumia elastica),and soil properties under them to elucidate their roles in the invasion of a tropical forest in Ghana.Methods Leaf litter production rates were determined using 36 mesh traps installed in the study area,while litter quality and soil physico-chemical properties were determined using standard protocols.A 6-month decomposition experiment using the litterbag technique was conducted to compare the decomposition rates of the species.Important Findings Litter production varied among the species and over time,with B.papyrifera producing 0.35-4.27 tons ha−1 y−1 from October to January;the other species produced 0.03^(-1).74 tons ha−1 y−1 over the same period.In the litterbag experiment,B.papyrifera recorded the lowest mass remaining(11-36%),followed by C.odorata(17-51%),F.elastica(31-55%)and C.mildbraedii(48-62%)in that order.Broussonetia papyrifera had the highest nitrogen(3.91%)and phosphorus(0.24%)but lowest lignin(12.20%)concentrations and the lowest C:N(10.87)ratio,indicating higher litter quality com-pared to the other species.Soil under B.paprifera was richest in phosphorus and nitrogen compared to the other species.Overall,our results indicate that the production of more nutrient-rich and rapidly decomposing leaf litter by B.papyrifera may constitute an important positive feedback mechanism driving its invasion and impacts in this tropical forest.展开更多
基金the Higher Education Commission(HEC)of Pakistan for obtaining a fellowship with grant number(1-8/HEC/HRD/2011/2162)
文摘Objective:To search for the most active antimicrobial and antioxidant sub-fractions related to traditional use of Ziziphus oxyphylla(Z.oxyphylla)and Cedrela serrata(C.serrata)in Pakistan against infectious and liver diseases.Methods:Factions of different polarity were tested in vitro for their antiprotozoal,antimalarial,antibacterial and antifungal activity against different pathogens.Cytotoxicity on MRC-5 cell lines(human lung fibroblasts)as well as,in vitro radical scavenging activity was evaluated using the 1,1-diphenyl-2-picrylhydrazyl radical assay.Results:The highest antiprotozoal activity was observed for the CHCl_(3)fractions of Z.oxyphylla roots and leaves,and C.serrata bark.The CHCl_(3)and Et OAc fractions of Z.oxyphylla roots,the CHCl_(3)fraction of Z.oxyphylla leaves,the Et OAc and the residual Me OH:H_(2)O fraction of C.serrata bark showed antibacterial activity against Staphylococcus aureus.The same residual Me OH:H_(2)O fraction of C.serrata bark was active against Candida albicans.The highest antioxidant activity was observed for the more hydrophilic Et OAc fractions of Z.oxyphylla leaves,C.serrata bark and leaves,and the residual Me OH:H_(2)O fraction of C.serrata bark.Conclusions:This study supports at least in part the traditional uses of these plants for antimicrobial purposes and against liver diseases.
文摘The association between the growth and water-use efficiency in three populations of C. odorata in Veracruz Mexico （La Antigua, Misantla and Catemaco） from two agroforestry system sites was studied, one in association with corn and another with pipiain. TRP （transpiration）, gs （stomatal conductivity）, CO2A （CO2 assimilation）, WUE （water use efficiency）, height, coverage and stem diameter were measured. The populafon of La Antigua associated with pipian presented the highest WUE （7.13 μmol CO2 m2-sl/mmol H20 m2.sl）, while the population of Catemaco reached high values （10 μmol CO2 m2 sl/mmol H20 m2-s1） in the site associated with corn, the populations established with corn presented a great growth in height and stem diameter. Based on the physiological responses and growth of the studied populations, the towns of La Antigua and Catemaco may be recommended for the establishment of commercial plantations.
文摘A study was conducted to examine the germination requirements of Cedrela odorata, Guaiacum sanctum and Calycophyllum candidissimum seeds, and the effect of light intensity on survival and growth of C. odorata and G. sanctum seedlings planted on open, partially-open and beneath the canopy of a dry forest in Nicaragua. The results show that germination of C. candidissimum seeds was signifi- cantly higher in light than darkness at constant temperatures ranging from 20-35℃. Both C. odorata and G. sanctum seeds germinated in light and darkness to a varying extent, and the optimal germination temperature was around 20-25℃. Alternating temperature regimes did not improve germination. Survival of G. sanctum seedlings was 70% beneath the canopy and 80% in the open, and its relative growth rate in collar diameter was 1.88 mm/month in the open and 1.42 mm/month in the understory. Survival was generally poor for C. odorata, particularly in the understory. We concluded that light is an absolute requirement for the germination of C. candidissimum seeds while germination of C. odorata and G. sanctum seeds are more sensitive to temperature. Owing to its high survival rate on open site, G. sanctum could serve as a candidate species for reforestation of degraded sites.
文摘Meliaceae plants are distinguished by the attack of the shootborer Hypsipyla grandella and also for the occurrence of limonoids, alkaloids and phenolic compounds. Such compounds extracted from leaves of Meliaceae species Cedrela odorata L., Swietenia macrophylla King, Khaya senegalensis, Toona ciliata, and C. odorata grafted onto T. ciliata plants, were tested on C. odorata leaf disks to determine their effects on survival and performance of H. grandella larvae. Larval survival was assessed 2, 10 and 25 days after starting the bioassays. Leaf consumption and weight gain per larva, days to pupa and to adult stages, pupal weight and length, and moth wing appearance were assessed for larval performance. The three compunds from the four Meliaceae species and the grafted combination affected (P ≤ 0.02) larval leaf consumption, weight gain, time to pupa and to adult stages, wing development and larval survival of H. grandella. Pupa weight (P = 0.78, F = 0.72, d.f. = 18, 160) and length (P = 0.48, F = 0.98, d.f. = 18, 160) were similar regardless of the coumpound used. Limonoid reduced larval survival on the three dates of evaluation. Alkaloids decreased leaf consumption, weight gain of larvae and time needed to reach pupa and adult stages. Alkaloids from T. ciliata and phenols from C. odorata were the best coumpounds to reduce leaf consumption and weight gain. Alkaloids from the grafted plants caused 20% of H. grandella adults to form abnormal wings.
基金supported by the Swedish International Development Agency (Sida)
文摘A study was conducted to examine the germination requirements of Cedrela odorata, Guaiacum sanctum and Calycophyllum candidissimum seeds, and the effect of light intensity on survival and growth of C.odorata and G.sanctum seedlings planted on open, partially-open and beneath the canopy of a dry forest in Nicaragua.The results show that germination of C.candidissimum seeds was significantly higher in light than darkness at constant temperatures ranging from 20-35oC.Both C.odorata and G.sanctum seeds germinated in light and darkness to a varying extent, and the optimal germination temperature was around 20-25oC.Alternating temperature regimes did not improve germination.Survival of G.sanctum seedlings was 70% beneath the canopy and 80% in the open, and its relative growth rate in collar diameter was 1.88 mm/month in the open and 1.42 mm/month in the understory.Survival was generally poor for C.odorata, particularly in the understory.We concluded that light is an absolute requirement for the germination of C.candidissimum seeds while germination of C.odorata and G.sanctum seeds are more sensitive to temperature.Owing to its high survival rate on open site, G.sanctum could serve as a candidate species for reforestation of degraded sites.
基金supported by the Council for Scientific and Technological Development(CNPq)the Coordination for the Improvement of Education Personnel(CAPES)。
文摘Water availability is a limiting factor for the establishment and development of forest species.To understand the appropriate conditions for the initial post-transplanting phase,it is necessary to understand the specific morphophysiological characteristics of the species,such as the leaf water potential and the efficiency of photosystemⅡ.We aimed to identify the influence of different water regimes on the morphophysiological aspects of young plants of two forest species(Cedrela.fissilis Vellozo and Eucalyptus saligna Sm.).Two greenhouse experiments were conducted for 28 days;one for each species.The design was completely randomized,and the treatments consisted of six different water regimes.Leaf water potential(Ψw)and chlorophyll a fluorescence were evaluated every 7 days.At the end of the experiment,morphological attributes(height,collection diameter,root volume,and dry matter)were measured and histological blades were made.The water demand of E.saligna was higher than that of C.fissilis and required greater replacement within a shorter period.The rehydration fromΨw=-2 Mpa allowed for a fast recovery of the young C.fissilis plants(Ψw=-1.5,Fv/Fm=0.796),which indicated good physiological plasticity of this species when submitted to water stress at a level that is not severe.The total dry matter allocation was different among species.Seedlings of E.saligna presented the best responses when submitted to a continuous water supply regime,while C.fissilis seedlings presented the best response under intermittent irrigation conditions.
文摘Aims Non-native invasive plants can alter soil chemistry through litter production and decomposition to facilitate their invasion.However,the important roles of these underlying processes in plant invasion remain poorly understood,particularly in tropical forest ecosys-tems.Here,we compared litter production,quality and decompos-ition of two invasive species(Broussonetia papyrifera and Cedrela odorata)and two co-occurring native species(Celtis mildbraedii and Funtumia elastica),and soil properties under them to elucidate their roles in the invasion of a tropical forest in Ghana.Methods Leaf litter production rates were determined using 36 mesh traps installed in the study area,while litter quality and soil physico-chemical properties were determined using standard protocols.A 6-month decomposition experiment using the litterbag technique was conducted to compare the decomposition rates of the species.Important Findings Litter production varied among the species and over time,with B.papyrifera producing 0.35-4.27 tons ha−1 y−1 from October to January;the other species produced 0.03^(-1).74 tons ha−1 y−1 over the same period.In the litterbag experiment,B.papyrifera recorded the lowest mass remaining(11-36%),followed by C.odorata(17-51%),F.elastica(31-55%)and C.mildbraedii(48-62%)in that order.Broussonetia papyrifera had the highest nitrogen(3.91%)and phosphorus(0.24%)but lowest lignin(12.20%)concentrations and the lowest C:N(10.87)ratio,indicating higher litter quality com-pared to the other species.Soil under B.paprifera was richest in phosphorus and nitrogen compared to the other species.Overall,our results indicate that the production of more nutrient-rich and rapidly decomposing leaf litter by B.papyrifera may constitute an important positive feedback mechanism driving its invasion and impacts in this tropical forest.
