目的检测多囊卵巢综合征(PCOS)合并代谢综合征(MS)患者血清miR-222-3p的表达水平,分析其与糖脂代谢指标的相关性。方法选取西安国际医学中心医院2020年1月至2021年12月收治的160例PCOS患者作为研究对象,根据是否合并MS分为单纯PCOS组(10...目的检测多囊卵巢综合征(PCOS)合并代谢综合征(MS)患者血清miR-222-3p的表达水平,分析其与糖脂代谢指标的相关性。方法选取西安国际医学中心医院2020年1月至2021年12月收治的160例PCOS患者作为研究对象,根据是否合并MS分为单纯PCOS组(105例)和PCOS+MS组(55例),比较2组空腹胰岛素(FINS)、空腹血糖(FPG)、餐后2 h血糖(2 h PG)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)水平,计算胰岛素抵抗指数(HOMA-IR)。采用滚环扩增(RCA)技术检测2组患者血清miR-222-3p表达水平。采用Pearson相关分析miR-222-3p表达水平与糖脂代谢指标的相关性。采用受试者工作特征(ROC)曲线分析miR-222-3p诊断PCOS患者发生MS的价值。结果PCOS+MS组血清FPG、2 h PG、FINS、TC、TG、LDL-C水平及HOMA-IR明显高于单纯PCOS组(P<0.05),HDL-C水平明显低于单纯PCOS组(P<0.05)。与单纯PCOS组比较,PCOS+MS组血清miR-222-3p表达水平明显升高(P<0.05)。PCOS患者miR-222-3p表达水平与FPG、2 h PG、FINS、HOMA-IR、TC、TG、LDL-C均呈正相关(P<0.05),与HDL-C呈负相关(P<0.05)。ROC曲线分析结果显示,miR-222-3p诊断PCOS发生MS的曲线下面积为0.806(95%CI:0.729~0.883),当截断值为2.32时,约登指数最大(0.526),此时的灵敏度为87.62%,特异度为65.00%。结论miR-222-3p在PCOS合并MS患者中表达水平较高,且与患者糖脂代谢紊乱密切相关。展开更多
目的研究222 nm紫外线消毒的有效性与安全性,为其实际应用提供科学依据。方法采用病毒定量灭活试验、载体定量杀菌试验、分层细胞片细胞安全性和一次性皮肤刺激试验,评价222 nm紫外线的病毒杀灭效果、杀菌效果和使用安全性。结果15 m J/...目的研究222 nm紫外线消毒的有效性与安全性,为其实际应用提供科学依据。方法采用病毒定量灭活试验、载体定量杀菌试验、分层细胞片细胞安全性和一次性皮肤刺激试验,评价222 nm紫外线的病毒杀灭效果、杀菌效果和使用安全性。结果15 m J/cm^(2)辐射剂量条件下,有机干扰物为0.3%BSA时,222 nm紫外线对玻片上大肠埃希菌和金黄色葡萄球菌的杀灭率分别为99.52%和99.97%;有机干扰物为3%BSA时,其对上述2种细菌的杀灭率分别为85.05%和99.49%。辐射剂量为30 m J/cm^(2)时,222 nm紫外线对玻片上甲型H1N1流感病毒、肠道病毒71型和脊髓灰质炎病毒Ⅰ型疫苗株的灭活率均可达99.9%;相同剂量照射分层细胞模型,上层与下层细胞的存活率分别约为70%和80%。辐射剂量为479 m J/cm^(2)时,222 nm紫外线照射家兔正常皮肤48 h,未观察到明显刺激性反应。结论222 nm紫外线对细菌繁殖体和病毒有良好的杀灭作用,其对下层细胞穿透性有限,无明显皮肤刺激性。展开更多
BACKGROUND Empathetic psychological care improves mood and enhances the quality of life in critically ill patients.AIM To study the impact of combining 222-nm ultraviolet(UV)disinfection with empathetic psychological ...BACKGROUND Empathetic psychological care improves mood and enhances the quality of life in critically ill patients.AIM To study the impact of combining 222-nm ultraviolet(UV)disinfection with empathetic psychological care on emotional states,nosocomial infection rates,and quality of life in critically ill patients.METHODS A total of 202 critically ill patients admitted to Beijing Ditan Hospital(December 2023 to May 2024)were randomly assigned to control(Ctrl,n=101)or observation groups(Obs,n=101).The Ctrl group received 222-nm UV disinfection and routine care,while the Obs group received 222-nm UV disinfection with empathetic psychological care.Emotional states[Self-Rating Anxiety Scale(SAS),Self-Rating Depression Scale(SDS)],hospital infection rates,quality of life(36-Item Short Form Health Survey),and patient satisfaction were evaluated.RESULTS At baseline,there were no significant differences in SAS and SDS scores between the groups(P>0.05).Following care,both groups demonstrated reductions in SAS and SDS scores,with the Obs group exhibiting a significantly greater reduction(P<0.05).The Obs group also experienced a significantly lower overall hospital infection rate(P<0.05).Similarly,while baseline 36-Item Short Form Health Survey scores did not differ significantly between the groups(P>0.05),post-care scores improved in both groups,with a greater improvement observed in the Obs group(P<0.05).Additionally,the Obs group reported higher patient satisfaction ratings(P<0.05).CONCLUSION The combination of 222-nm UV disinfection and empathetic psychological care improves emotional states,reduces hospital infection rates,enhances the quality of life,and increases patient satisfaction among critically ill patients.展开更多
文摘目的检测多囊卵巢综合征(PCOS)合并代谢综合征(MS)患者血清miR-222-3p的表达水平,分析其与糖脂代谢指标的相关性。方法选取西安国际医学中心医院2020年1月至2021年12月收治的160例PCOS患者作为研究对象,根据是否合并MS分为单纯PCOS组(105例)和PCOS+MS组(55例),比较2组空腹胰岛素(FINS)、空腹血糖(FPG)、餐后2 h血糖(2 h PG)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)水平,计算胰岛素抵抗指数(HOMA-IR)。采用滚环扩增(RCA)技术检测2组患者血清miR-222-3p表达水平。采用Pearson相关分析miR-222-3p表达水平与糖脂代谢指标的相关性。采用受试者工作特征(ROC)曲线分析miR-222-3p诊断PCOS患者发生MS的价值。结果PCOS+MS组血清FPG、2 h PG、FINS、TC、TG、LDL-C水平及HOMA-IR明显高于单纯PCOS组(P<0.05),HDL-C水平明显低于单纯PCOS组(P<0.05)。与单纯PCOS组比较,PCOS+MS组血清miR-222-3p表达水平明显升高(P<0.05)。PCOS患者miR-222-3p表达水平与FPG、2 h PG、FINS、HOMA-IR、TC、TG、LDL-C均呈正相关(P<0.05),与HDL-C呈负相关(P<0.05)。ROC曲线分析结果显示,miR-222-3p诊断PCOS发生MS的曲线下面积为0.806(95%CI:0.729~0.883),当截断值为2.32时,约登指数最大(0.526),此时的灵敏度为87.62%,特异度为65.00%。结论miR-222-3p在PCOS合并MS患者中表达水平较高,且与患者糖脂代谢紊乱密切相关。
文摘目的研究222 nm紫外线消毒的有效性与安全性,为其实际应用提供科学依据。方法采用病毒定量灭活试验、载体定量杀菌试验、分层细胞片细胞安全性和一次性皮肤刺激试验,评价222 nm紫外线的病毒杀灭效果、杀菌效果和使用安全性。结果15 m J/cm^(2)辐射剂量条件下,有机干扰物为0.3%BSA时,222 nm紫外线对玻片上大肠埃希菌和金黄色葡萄球菌的杀灭率分别为99.52%和99.97%;有机干扰物为3%BSA时,其对上述2种细菌的杀灭率分别为85.05%和99.49%。辐射剂量为30 m J/cm^(2)时,222 nm紫外线对玻片上甲型H1N1流感病毒、肠道病毒71型和脊髓灰质炎病毒Ⅰ型疫苗株的灭活率均可达99.9%;相同剂量照射分层细胞模型,上层与下层细胞的存活率分别约为70%和80%。辐射剂量为479 m J/cm^(2)时,222 nm紫外线照射家兔正常皮肤48 h,未观察到明显刺激性反应。结论222 nm紫外线对细菌繁殖体和病毒有良好的杀灭作用,其对下层细胞穿透性有限,无明显皮肤刺激性。
基金Supported by Beijing Ditan Hospital Affiliated to Capital Medical University“Sailing Plan”,No.DTQH-202405.
文摘BACKGROUND Empathetic psychological care improves mood and enhances the quality of life in critically ill patients.AIM To study the impact of combining 222-nm ultraviolet(UV)disinfection with empathetic psychological care on emotional states,nosocomial infection rates,and quality of life in critically ill patients.METHODS A total of 202 critically ill patients admitted to Beijing Ditan Hospital(December 2023 to May 2024)were randomly assigned to control(Ctrl,n=101)or observation groups(Obs,n=101).The Ctrl group received 222-nm UV disinfection and routine care,while the Obs group received 222-nm UV disinfection with empathetic psychological care.Emotional states[Self-Rating Anxiety Scale(SAS),Self-Rating Depression Scale(SDS)],hospital infection rates,quality of life(36-Item Short Form Health Survey),and patient satisfaction were evaluated.RESULTS At baseline,there were no significant differences in SAS and SDS scores between the groups(P>0.05).Following care,both groups demonstrated reductions in SAS and SDS scores,with the Obs group exhibiting a significantly greater reduction(P<0.05).The Obs group also experienced a significantly lower overall hospital infection rate(P<0.05).Similarly,while baseline 36-Item Short Form Health Survey scores did not differ significantly between the groups(P>0.05),post-care scores improved in both groups,with a greater improvement observed in the Obs group(P<0.05).Additionally,the Obs group reported higher patient satisfaction ratings(P<0.05).CONCLUSION The combination of 222-nm UV disinfection and empathetic psychological care improves emotional states,reduces hospital infection rates,enhances the quality of life,and increases patient satisfaction among critically ill patients.
