In order to study the difference between cauliflower varieties with ball well protected and unprotected in terms of pesticide residue and its causes, Jinpin 70, a new male sterile cauliflower variety with ball well pr...In order to study the difference between cauliflower varieties with ball well protected and unprotected in terms of pesticide residue and its causes, Jinpin 70, a new male sterile cauliflower variety with ball well protected, and Xuebao with ball unprotected were sprayed with high-toxic pesticide phorate and efficient bio-pesticide emamectin benzoate at a regular dose to investigate the impact of different cauliflower varieties on the pesticide residue. The results showed that from the 3rd to the 5th day after spraying, phorate and emamectin benzoate residues in Jinpin 70 with ball well protected reduced by 76.47%-87.65% and 67.86%-80.82% re- spectively compared with Xubao, and decreased by 9.23%-81.82% and 57.14%- 79.84% respectively compared with Jinpin 70 with ball unprotected. The results above demonstrates that inner leaves of cauliflower with ball well protected can prevent the ball from being polluted by phorate and emamectin benzoate, thereby improving its edible safety.展开更多
Cauliflower is an important variety of Brassica oleracea and is planted worldwide.Here,the high-quality genome sequence of cauliflower was reported.The assembled cauliflower genome was 584.60 Mb in size,with a contig ...Cauliflower is an important variety of Brassica oleracea and is planted worldwide.Here,the high-quality genome sequence of cauliflower was reported.The assembled cauliflower genome was 584.60 Mb in size,with a contig N50 of 2.11 Mb,and contained 47,772 genes;56.65%of the genome was composed of repetitive sequences.Among these sequences,long terminal repeats(LTRs)were the most abundant(32.71%of the genome),followed by transposable elements(TEs)(12.62%).Comparative genomic analysis confirmed that after an ancient paleohexaploidy(γ)event,cauliflower underwent two whole-genome duplication(WGD)events shared with Arabidopsis and an additional whole-genome triplication(WGT)event shared with other Brassica species.The present cultivated cauliflower diverged from the ancestral B.oleracea species~3.0 million years ago(Mya).The speciation of cauliflower(~2.0 Mya)was later than that of B.oleracea L.var.capitata(approximately 2.6 Mya)and other Brassica species(over 2.0 Mya).Chromosome no.03 of cauliflower shared the most syntenic blocks with the A,B,and C genomes of Brassica species and its eight other chromosomes,implying that chromosome no.03 might be the most ancient one in the cauliflower genome,which was consistent with the chromosome being inherited from the common ancestor of Brassica species.In addition,2,718 specific genes,228 expanded genes,2 contracted genes,and 1,065 positively selected genes in cauliflower were identified and functionally annotated.These findings provide new insights into the genomic diversity of Brassica species and serve as a valuable reference for molecular breeding of cauliflower.展开更多
Cowpea Trypsin Inhibitor (CpTI) gene was transferred into the cotyle dons and hypocotyls of cauliflower by Agrobacterium-mediated transformation met hod. The best selective concentration of kanamycin (kan) was 15 mg L...Cowpea Trypsin Inhibitor (CpTI) gene was transferred into the cotyle dons and hypocotyls of cauliflower by Agrobacterium-mediated transformation met hod. The best selective concentration of kanamycin (kan) was 15 mg L-1. The con centration of carbencillin (carb) was 500 mg L-1. 14 transgenic cauliflower pla nts were obtained. The putative transformants were assayed by PCR and Southern b lotting analysis. The results indicated that CpTI gene was transferred into caul iflower successfully.展开更多
Cauliflower is popular vegetable in Pakistan and it is severely attacked by Pieris brassicae (L). There are different cauliflower cultivars available in this area. The aim of this study was to determine the developmen...Cauliflower is popular vegetable in Pakistan and it is severely attacked by Pieris brassicae (L). There are different cauliflower cultivars available in this area. The aim of this study was to determine the developmental response of Pieris brassicae on ten cauliflower cultivars. An experiment was conducted during 2012-13 at the Entomology section of the Agriculture Research Institute Tarnab Peshawar under laboratory conditions of 20°C ± 2°C, 50% ± 5% RH and 12:12h photoperiod. Pieris brassicae eggs were collected from a cauliflower field ARI Tarnab and the larvae placed after hatching on fresh leaves often cauliflower cultivars. The results showed that the larval development of P. brassicae was short (35 days), longer larval length (32.41 mm), larval mortality was low (6.6%) and pupal weight was high (0.50 g) on Clima cultivar. On the other hand, a longer larval developmental period (37 days), higher larval mortality (86.66%), shorter larval length (24.55 mm) and lower pupal weight (0.42 g) were recorded on cultivar AX-2034.展开更多
[Objective]The paper was to screen out effective insecticides against main lepidopteran pests of cauliflower.[Method]Field trials were carried out in 2018,and 11 treatments were designed in the test.The population dec...[Objective]The paper was to screen out effective insecticides against main lepidopteran pests of cauliflower.[Method]Field trials were carried out in 2018,and 11 treatments were designed in the test.The population decline rate and corrected control effect were calculated.[Result]16%Indoxacarb+tebufenozide EC and 35%methoxyfenozide+indoxacarb SC had quick bioactivity and persistence,and the control effects at 1 and 10 d post spraying were greater than 85%.11%Emamectin benzoate+lufenuron ME,1 billion PIB/mL of Autographa californica NPV SC and 10%alpha-cypermethrin+lufenuron EC had good persistence,and the control effect at 10 d post spraying was greater than 90%.[Conclusion]Compound preparations of indoxacarb and tebufenozide or methoxyfenozide are recommended for controlling lepidopteran pests of cauliflower.展开更多
The promoter region of cauliflower mosaic virus (CaMV) 35s RNA was employed to construct an intermediate expression vector which can be used in Ti plasmid system of Agro-bacterium tumefaciens. The original plasmid, wh...The promoter region of cauliflower mosaic virus (CaMV) 35s RNA was employed to construct an intermediate expression vector which can be used in Ti plasmid system of Agro-bacterium tumefaciens. The original plasmid, which contains a polylinker between CaMV 35s RNA and its 3' termination signal in pUC18 was modified to have another antibiotic resistance marker (kanamycin resistance gene Kmr) to facilitate the selection of recombinant with Ti plasmid. Octopine synthase (ocs) structural gene was inserted into this vector downstream of CaMV 35s RNA promoter. This chimaeric gene was introduced into integrative Ti plasmid vector pGV3850, and then transformed into Nicotiana tobaccum cells. A binary plasmid vector was also used to introduce the chimaeric gene into tobacco cells. In both cases, the expression of ocs gene was demonstrated. The amount of oc-topine was much more than the nopaline synthesized by no-paline synthase (nos) gene transferred at the same time with Ti plasmid vector. This demonstrated that CaMV 35s RNA promoter is stronger in transcriptional function than the promoter of nos in tobacco cells.展开更多
The present study described the enhancement of the nutritional values of wheat and rice crackers by adding cauliflowers. The results showed that 25% - 75% cauliflower replaced in crackers increased the consumer accept...The present study described the enhancement of the nutritional values of wheat and rice crackers by adding cauliflowers. The results showed that 25% - 75% cauliflower replaced in crackers increased the consumer acceptability, nutritional value, antioxidant activity, minerals, and anti-carcinogenic effect. Moreover, it also improved the functional properties and sensory quality. The results revealed that the raw samples of cauliflower recorded the highest value of moisture, ash, protein and fiber 89.29%, 7.68%, 19.04% and 10.18% on dry weight, respectively. The crackers formula of cauliflower: wheat 75%:25% and cauliflower: rice 75%:25% recorded the highest values in ash, protein, fat, and fiber. The data showed that increasing cauliflower percentage in crackers formula causing a parallel increase in all determined chemical composition. Cauliflower samples exhibited the highest antioxidant activity (92.94%). Also, crackers samples of cauliflower: wheat 75%:25% crackers, recorded 57.6% for antioxidant activity. The highest values of phenols and flavonoids for cauliflower cracker samples (cauliflower: wheat 75%:25% and cauliflower: rice 50%:50%) have increased up to (5.75 and 5.49) respectively. The results showed that the higher mineral content is the sulfur (635.96 mg/100g) in raw cauliflower, while (1.00 mg/100g, and 0.99 mg/100g sulfur content) in wheat and rice respectively. Moreover, the result found that as the cauliflower percentage increases, the minerals contents especially sulfur increase. Furthermore, the cauliflower displayed moderate activity for human hepatocellular carcinoma, colon cancer and intestinal epithelial barrier treatment, (IC</span><sub><span style="font-family:Verdana;">50</span></sub></span><span style="font-family:""> </span><span style="font-family:Verdana;">= 183.4 ± 6.8, 175.8 ± 7.1 and 207.2 ± 8.5 μg/mL, respectively). There were significant differences in the taste property;the highest degree of acceptance of the taste was obtained to crackers samples which were replaced with cauliflower more than the control.展开更多
The biological half-life and final residue levels of indoxacarb and pyridalyl were determined in cauliflower over a 10-day cultivation period following applications of a standard (100 g a.i.ha–1 and 200 g a.i.·h...The biological half-life and final residue levels of indoxacarb and pyridalyl were determined in cauliflower over a 10-day cultivation period following applications of a standard (100 g a.i.ha–1 and 200 g a.i.·ha–1, respectively) and double dose (200 g a.i.·ha–1 and 400 g a.i.·ha–1, respectively). The residue levels were analyzed by gas chromatography with recovery ranging from 92.1% to 109.7%. The biological half-lives of indoxacarb and pyridalyl were 6.33 and 7.74 days for the standard dose, and 6.26 and 7.44 days for the double dose, respectively. The initial and persisting concentrations of indoxacarb and pyridalyl were all below the Korean maximum residue limits for broccoli of 1.0 mgkg–1 and 3.0 mgkg–1, respectively.展开更多
A rapid, simple and an efficient method for the determination of indoxacarb in cauliflower and soil samples was developed and validated using QuEChERS technique (Quick, Easy, Cheap, Effective, Rugged and Safe). Recove...A rapid, simple and an efficient method for the determination of indoxacarb in cauliflower and soil samples was developed and validated using QuEChERS technique (Quick, Easy, Cheap, Effective, Rugged and Safe). Recoveries at four different spiking concentrations of 0.01, 0.05, 0.1 and 0.2 mg kg–1 ranged from 87 to 96% were achieved with good repeatability and RSD of 1% - 6%. The average initial deposits of 0.23 and 0.45 mg kg–1 were observed after last application of indoxacarb @ 52.2 and 104.4 g. a.i. ha–1 at recommended and double the recommended dosages, respectively. The residues in cauliflower dissipated below its LOQ of 0.01 mg kg–1 after 7 days and its half-life periods were observed to be 1.12 and 1.31 days, respectively, at single and double the dosages. Keeping in view 80 g consumption of cauliflower curds per day for a 55 kg person, theoretical maximum residue contribution (TMRC) of indoxacarb when calculated from maximum residues observed on 0 day samples at recommended and double the recommended dosages, respectively, were found to be 20.8 and 36.8 μg in comparison to its acceptable daily intake (ADI) of 550 μg, which is quite safe.展开更多
The results of researches aimed to establish the optimum growing space and density of standing of plants at cultivation of cauliflower seeds in Uzbekistan are stated in the article. Researches were spent in the suburb...The results of researches aimed to establish the optimum growing space and density of standing of plants at cultivation of cauliflower seeds in Uzbekistan are stated in the article. Researches were spent in the suburb of Tashkent with a mid-early grade ＂Domestic＂. In experiences were compared 5 densities of standing （31746, 35714, 40806, 47620 and 57142 pieces/hectare） received at planting of sprouts with row-spacing of 70 sm and distance between plants in a row 25, 30, 35, 40 and 45 sm. Experiences were pawned in quadruple frequency with the area of an allotment 10 M2. 60-65 day sprouts were planted under film tunnel shelters on February 11-12. Film shelters were kept over plants till the end of March. Seed plants were grown up without formation. Experiences were accompanied by phonological control, calculation of leaves quantity, an establishment of average weight of a head, definition of quantity of the plants, which have given seeds, the account of a crop of seeds from a bush and of a unit of area. It has been defined that reception of seeds of a cauliflower at cultivation under film tunnels is quite possible in Uzbekistan. It is established that with representation of the big growing space the plants form more leaves, earlier and larger form the heads, develop the testicles acceleration and increase their seed efficiency. It has been revealed that the more plants thickness is, the bigger share of them does not give seeds. In this connection the greatest crop of seeds has been received at growing space 70x45 sm at which 31.7 thousand Pl/ha had been planted. It is proved that in the conditions of Uzbekistan it is possible to cultivate high-grade seeds of cauliflower with productivity of 127-147 kg/ha.展开更多
In the present study,self-propelled cabbage/cauliflower harvester was designed,developed and evaluated.The machine consisted of different components like engine,frame,shearing(cutting)unit and power transmission unit....In the present study,self-propelled cabbage/cauliflower harvester was designed,developed and evaluated.The machine consisted of different components like engine,frame,shearing(cutting)unit and power transmission unit.The power transmission unit consisted of main clutch,shearing blade operating clutch,belt drive unit,chain and sprocket drive,universal joint and cutter blade assembly.The main working principle of harvester is based on shearing of crop stem against high-speed rotating blade.The power from the engine is transmitted by belt-pulley drive unit to transmission shaft on which chain and sprocket is mounted on one side and then power is transmitted to shearing blade coupling with the help of a stationary pulley and fixed socket.Average mean head diameter of the selected cabbage and cauliflower was 89.5±15.24 mm and 107.5±15.24 mm,respectively.Average mean stem(plant)diameter of the selected cabbage and cauliflower was 18±4.85 mm and 21.5±3.08 mm,respectively.The shearing force increased with increase in diameter of stem.The optimum performance of the machine was achieved when it was operated at 1.5 km/h forward speed and the shearing blade moving at speed of 147 rpm.The mean field capacity for developed prototype was observed as 0.063 ha/h and 0.053 in case of cabbage and cauliflower,respectively with field efficiency of 91.97 and 90.48%.The average head damage was negligible(0.15%)for both the crops.The average untrimmed percentage with developed harvester was 3.2 and 3.0%in case of cabbage and cauliflower crop,respectively.The developed machine helps to increase the field capacity in cabbage/cauliflower harvesting due to 7-times more capacity and 50%cheaper compared to traditional method of cabbage/cauliflower harvesting.At the operating condition of forward speed(1.5 km/h)and shearing blade speed(147 rpm),the machine could harvest 0.5 ha of cabbage and 0.42 ha of cauliflower farm per day of 8-h.This same task would have required between 15 labour per day if entirely done manually.展开更多
This research explored a novel multimodal approach for disease management in cauliflower crops.With the rising challenges in sustainable agriculture,the research focused on a patch spraying method to control disease a...This research explored a novel multimodal approach for disease management in cauliflower crops.With the rising challenges in sustainable agriculture,the research focused on a patch spraying method to control disease and reduce crop losses and environmental impact.For non-destructive disease assessment,a spectral sensor was used to collect spectral information from diseased and healthy cauliflower parts.The spectral data sets were analyzed using decision tree and support vector machine(SVM)algorithms to identify the most accurate model for distinguishing diseased and healthy plants.The chosen model was integrated with a low-volume sprayer(50-150 L·ha^(-1)),equipped with an electronic control unit for targeted spraying based on sensor-detected regions.The decision tree model achieved 89.9% testing accuracy,while the SVM model achieved 96.7% accuracy using hyperparameters:cost of 10.0 and tolerance of 0.001.The research successfully demonstrated the integration of spectral sensors,machine learning,and targeted spraying technology for precise input application.Additionally,the optimized sprayer achieved a 72.5% reduction in chemical usage and a significant time-saving of 21.0% compared to a standard sprayer for black rot-infested crops.These findings highlight the potential efficiency and resource conservation benefits of innovative sprayer technology in precision agriculture and disease management.展开更多
I. INTRODUCTION Cauliflower mosaic virus (CaMV)is a double stranded DNA virus. It attracted attention in the pasf few years because of its special replication process and its possible use as a vector in plant genetic ...I. INTRODUCTION Cauliflower mosaic virus (CaMV)is a double stranded DNA virus. It attracted attention in the pasf few years because of its special replication process and its possible use as a vector in plant genetic engineering. For a further study it is important to get the infectious clone of the CaMV genome at first. Although several infectious clones have been展开更多
Cauliflower(Brassica oleracea var.botrytis L.),a cruciferous vegetable belonging to the Brassicaceae family,is a vital component of global vegetable production systems.Renowned for its high nutritional value,which inc...Cauliflower(Brassica oleracea var.botrytis L.),a cruciferous vegetable belonging to the Brassicaceae family,is a vital component of global vegetable production systems.Renowned for its high nutritional value,which includes substantial levels of protein,lipids,carbohydrates,dietary fiber,vitamins,and minerals,it provides significant health benefits.Taste components,particularly the aroma,influence the culinary and consumption experience,thereby affecting human food preferences(Lu and Zhu 2022).Current breeding initiatives prioritize the development of novel cultivars with enhanced organoleptic traits while maintaining yield stability,addressing the growing demand for premium horticultural commodities in global fresh produce markets.展开更多
In this study,we used the modified CRISPR/Cas9 system to produce targeted point mutations in cauliflower.Acetolactate synthase(ALS)and Centromere-specific histone H3 variant(CENH3)genes were selected as the base-editi...In this study,we used the modified CRISPR/Cas9 system to produce targeted point mutations in cauliflower.Acetolactate synthase(ALS)and Centromere-specific histone H3 variant(CENH3)genes were selected as the base-editing targets and hypocotyls of cauliflower were used as explants.For ALS gene,a C-to-T conversion in the Pro182 codon(CCT)can alter the encoded amino acid,likely resulting in herbicide resistance,and a C-to-T mutation in the Leu133 codon(CTT)in the CENH3 gene may produce a haploid inducer.Results indicated that the transformation efficiency was 1.8%–4.5%and the mutation efficiencies for the ALS and CENH3 genes were approximately 22%and 87%,respectively.The ALS mutant cauliflower showed strong herbicide resistance,with possible immediate implications for broadleaf weed control in cauliflower fields.展开更多
Investigation was made to confirm the stability of drought and salt stress tolerance in cauliflower (Brassica oleracea var.botrytis) mutants after regeneration and micropropagation. The N-nitroso-N-ethyleurea (NEU) an...Investigation was made to confirm the stability of drought and salt stress tolerance in cauliflower (Brassica oleracea var.botrytis) mutants after regeneration and micropropagation. The N-nitroso-N-ethyleurea (NEU) and N-nitroso-N-methylurea (NMU) induced mutants of cauliflower were created and screened for drought and salt stress tolerance. The highly tolerant mutants were selected, regenerated by tissue culture techniques, screened again for drought and salt tolerance under in-vitro and in-vivo conditions, correlated the response of in-vitro and in-vivo plants within a clone. Free proline levels in clones were correlated with stress tolerance. Results confirmed the persistence of mutations in clones with enhanced resistance levels to stresses over control plants. The regenerated in-vitro and in-vivo plants within a clone showed a positive significant correlation for drought (R2 = 0.663) and salt (R2 = 0.647) resistance that confirms the stability of mutation in clones after generations. Proline showed a positive and significant correlation with drought (R2 = 0.524) and salt (R2 = 0.786) tolerance. Conclusively, drought and salt resistance can be successfully enhanced in cauliflower by chemical mutagenesis. Further molecular analysis is recommended to study these mutants.展开更多
<strong>Introduction:</strong> Buschke Lowenstein Tumor (BLT) is a tumor, belonging to the group of verrucous carcinomas. It is endowed with an unpredictable potential of recurrence and degeneration. The a...<strong>Introduction:</strong> Buschke Lowenstein Tumor (BLT) is a tumor, belonging to the group of verrucous carcinomas. It is endowed with an unpredictable potential of recurrence and degeneration. The aim of the work is to present the anatomical and therapeutic characteristics of BLT at the University Hospital of National Reference of N’Djamena in Chad. <strong>Patients and Method:</strong> Prospective descriptive study of 13 patients with BLT, from May 2009 to April 2019 in the department of urology was included. The patients more than 15, carriers of TBL located at: external genitalia, perineum and anal margin whose clinical, paraclinical assessment allowed the diagnosis of BLT, management and follow-up at the said service. Respect for the patient’s identity and consent, the patient’s consent is obtained and the anonymity of the photographs is observed. <strong>Result:</strong> A total of 13 patients had BLT, 11 males and 2 females. The average age of the patients was 32.5 years. The lesions were cauliflower-like and located on the vulva (n = 2), the penis, the perineum, the anorectal region in men (n = 11). Serology was positive for: HIV (n = 3), chlamydia (n = 4). The management was surgical by cauterization and excision. Cystoscopy and rectoscope were normal. The cure was obtained in all patients. Two patients had a recurrence at 5 months requiring a second successful cure. <strong>Conclusion:</strong> BLT is a frequent pathology in the urology department of the University Hospital of National Reference of N’Djamena in Chad. The surgical treatment by “cauterization exeresis” gives a better treatment.展开更多
基金Supported by the Technology System Project of National Vegetable Industry(CARS-25-A-13)Science and Technology Planning Project of Tianjin City(12ZCDZNC03600)Major Science and Technology Engineering Project of Tianjin City(12ZCDZNC03500)~~
文摘In order to study the difference between cauliflower varieties with ball well protected and unprotected in terms of pesticide residue and its causes, Jinpin 70, a new male sterile cauliflower variety with ball well protected, and Xuebao with ball unprotected were sprayed with high-toxic pesticide phorate and efficient bio-pesticide emamectin benzoate at a regular dose to investigate the impact of different cauliflower varieties on the pesticide residue. The results showed that from the 3rd to the 5th day after spraying, phorate and emamectin benzoate residues in Jinpin 70 with ball well protected reduced by 76.47%-87.65% and 67.86%-80.82% re- spectively compared with Xubao, and decreased by 9.23%-81.82% and 57.14%- 79.84% respectively compared with Jinpin 70 with ball unprotected. The results above demonstrates that inner leaves of cauliflower with ball well protected can prevent the ball from being polluted by phorate and emamectin benzoate, thereby improving its edible safety.
基金supported by grants from the National Key Research and Development Program of China(2017YFD0101805)the Modern Agro-Industry Technology Research System,China(CARS-23-A-07)+1 种基金the Vegetable Modern Agro-Industry Technology Research System,Tianjin(ITTVRS2017004)the Natural Science Foundation of China(No.31872115).
文摘Cauliflower is an important variety of Brassica oleracea and is planted worldwide.Here,the high-quality genome sequence of cauliflower was reported.The assembled cauliflower genome was 584.60 Mb in size,with a contig N50 of 2.11 Mb,and contained 47,772 genes;56.65%of the genome was composed of repetitive sequences.Among these sequences,long terminal repeats(LTRs)were the most abundant(32.71%of the genome),followed by transposable elements(TEs)(12.62%).Comparative genomic analysis confirmed that after an ancient paleohexaploidy(γ)event,cauliflower underwent two whole-genome duplication(WGD)events shared with Arabidopsis and an additional whole-genome triplication(WGT)event shared with other Brassica species.The present cultivated cauliflower diverged from the ancestral B.oleracea species~3.0 million years ago(Mya).The speciation of cauliflower(~2.0 Mya)was later than that of B.oleracea L.var.capitata(approximately 2.6 Mya)and other Brassica species(over 2.0 Mya).Chromosome no.03 of cauliflower shared the most syntenic blocks with the A,B,and C genomes of Brassica species and its eight other chromosomes,implying that chromosome no.03 might be the most ancient one in the cauliflower genome,which was consistent with the chromosome being inherited from the common ancestor of Brassica species.In addition,2,718 specific genes,228 expanded genes,2 contracted genes,and 1,065 positively selected genes in cauliflower were identified and functionally annotated.These findings provide new insights into the genomic diversity of Brassica species and serve as a valuable reference for molecular breeding of cauliflower.
文摘Cowpea Trypsin Inhibitor (CpTI) gene was transferred into the cotyle dons and hypocotyls of cauliflower by Agrobacterium-mediated transformation met hod. The best selective concentration of kanamycin (kan) was 15 mg L-1. The con centration of carbencillin (carb) was 500 mg L-1. 14 transgenic cauliflower pla nts were obtained. The putative transformants were assayed by PCR and Southern b lotting analysis. The results indicated that CpTI gene was transferred into caul iflower successfully.
文摘Cauliflower is popular vegetable in Pakistan and it is severely attacked by Pieris brassicae (L). There are different cauliflower cultivars available in this area. The aim of this study was to determine the developmental response of Pieris brassicae on ten cauliflower cultivars. An experiment was conducted during 2012-13 at the Entomology section of the Agriculture Research Institute Tarnab Peshawar under laboratory conditions of 20°C ± 2°C, 50% ± 5% RH and 12:12h photoperiod. Pieris brassicae eggs were collected from a cauliflower field ARI Tarnab and the larvae placed after hatching on fresh leaves often cauliflower cultivars. The results showed that the larval development of P. brassicae was short (35 days), longer larval length (32.41 mm), larval mortality was low (6.6%) and pupal weight was high (0.50 g) on Clima cultivar. On the other hand, a longer larval developmental period (37 days), higher larval mortality (86.66%), shorter larval length (24.55 mm) and lower pupal weight (0.42 g) were recorded on cultivar AX-2034.
文摘[Objective]The paper was to screen out effective insecticides against main lepidopteran pests of cauliflower.[Method]Field trials were carried out in 2018,and 11 treatments were designed in the test.The population decline rate and corrected control effect were calculated.[Result]16%Indoxacarb+tebufenozide EC and 35%methoxyfenozide+indoxacarb SC had quick bioactivity and persistence,and the control effects at 1 and 10 d post spraying were greater than 85%.11%Emamectin benzoate+lufenuron ME,1 billion PIB/mL of Autographa californica NPV SC and 10%alpha-cypermethrin+lufenuron EC had good persistence,and the control effect at 10 d post spraying was greater than 90%.[Conclusion]Compound preparations of indoxacarb and tebufenozide or methoxyfenozide are recommended for controlling lepidopteran pests of cauliflower.
文摘The promoter region of cauliflower mosaic virus (CaMV) 35s RNA was employed to construct an intermediate expression vector which can be used in Ti plasmid system of Agro-bacterium tumefaciens. The original plasmid, which contains a polylinker between CaMV 35s RNA and its 3' termination signal in pUC18 was modified to have another antibiotic resistance marker (kanamycin resistance gene Kmr) to facilitate the selection of recombinant with Ti plasmid. Octopine synthase (ocs) structural gene was inserted into this vector downstream of CaMV 35s RNA promoter. This chimaeric gene was introduced into integrative Ti plasmid vector pGV3850, and then transformed into Nicotiana tobaccum cells. A binary plasmid vector was also used to introduce the chimaeric gene into tobacco cells. In both cases, the expression of ocs gene was demonstrated. The amount of oc-topine was much more than the nopaline synthesized by no-paline synthase (nos) gene transferred at the same time with Ti plasmid vector. This demonstrated that CaMV 35s RNA promoter is stronger in transcriptional function than the promoter of nos in tobacco cells.
文摘The present study described the enhancement of the nutritional values of wheat and rice crackers by adding cauliflowers. The results showed that 25% - 75% cauliflower replaced in crackers increased the consumer acceptability, nutritional value, antioxidant activity, minerals, and anti-carcinogenic effect. Moreover, it also improved the functional properties and sensory quality. The results revealed that the raw samples of cauliflower recorded the highest value of moisture, ash, protein and fiber 89.29%, 7.68%, 19.04% and 10.18% on dry weight, respectively. The crackers formula of cauliflower: wheat 75%:25% and cauliflower: rice 75%:25% recorded the highest values in ash, protein, fat, and fiber. The data showed that increasing cauliflower percentage in crackers formula causing a parallel increase in all determined chemical composition. Cauliflower samples exhibited the highest antioxidant activity (92.94%). Also, crackers samples of cauliflower: wheat 75%:25% crackers, recorded 57.6% for antioxidant activity. The highest values of phenols and flavonoids for cauliflower cracker samples (cauliflower: wheat 75%:25% and cauliflower: rice 50%:50%) have increased up to (5.75 and 5.49) respectively. The results showed that the higher mineral content is the sulfur (635.96 mg/100g) in raw cauliflower, while (1.00 mg/100g, and 0.99 mg/100g sulfur content) in wheat and rice respectively. Moreover, the result found that as the cauliflower percentage increases, the minerals contents especially sulfur increase. Furthermore, the cauliflower displayed moderate activity for human hepatocellular carcinoma, colon cancer and intestinal epithelial barrier treatment, (IC</span><sub><span style="font-family:Verdana;">50</span></sub></span><span style="font-family:""> </span><span style="font-family:Verdana;">= 183.4 ± 6.8, 175.8 ± 7.1 and 207.2 ± 8.5 μg/mL, respectively). There were significant differences in the taste property;the highest degree of acceptance of the taste was obtained to crackers samples which were replaced with cauliflower more than the control.
文摘The biological half-life and final residue levels of indoxacarb and pyridalyl were determined in cauliflower over a 10-day cultivation period following applications of a standard (100 g a.i.ha–1 and 200 g a.i.·ha–1, respectively) and double dose (200 g a.i.·ha–1 and 400 g a.i.·ha–1, respectively). The residue levels were analyzed by gas chromatography with recovery ranging from 92.1% to 109.7%. The biological half-lives of indoxacarb and pyridalyl were 6.33 and 7.74 days for the standard dose, and 6.26 and 7.44 days for the double dose, respectively. The initial and persisting concentrations of indoxacarb and pyridalyl were all below the Korean maximum residue limits for broccoli of 1.0 mgkg–1 and 3.0 mgkg–1, respectively.
文摘A rapid, simple and an efficient method for the determination of indoxacarb in cauliflower and soil samples was developed and validated using QuEChERS technique (Quick, Easy, Cheap, Effective, Rugged and Safe). Recoveries at four different spiking concentrations of 0.01, 0.05, 0.1 and 0.2 mg kg–1 ranged from 87 to 96% were achieved with good repeatability and RSD of 1% - 6%. The average initial deposits of 0.23 and 0.45 mg kg–1 were observed after last application of indoxacarb @ 52.2 and 104.4 g. a.i. ha–1 at recommended and double the recommended dosages, respectively. The residues in cauliflower dissipated below its LOQ of 0.01 mg kg–1 after 7 days and its half-life periods were observed to be 1.12 and 1.31 days, respectively, at single and double the dosages. Keeping in view 80 g consumption of cauliflower curds per day for a 55 kg person, theoretical maximum residue contribution (TMRC) of indoxacarb when calculated from maximum residues observed on 0 day samples at recommended and double the recommended dosages, respectively, were found to be 20.8 and 36.8 μg in comparison to its acceptable daily intake (ADI) of 550 μg, which is quite safe.
文摘The results of researches aimed to establish the optimum growing space and density of standing of plants at cultivation of cauliflower seeds in Uzbekistan are stated in the article. Researches were spent in the suburb of Tashkent with a mid-early grade ＂Domestic＂. In experiences were compared 5 densities of standing （31746, 35714, 40806, 47620 and 57142 pieces/hectare） received at planting of sprouts with row-spacing of 70 sm and distance between plants in a row 25, 30, 35, 40 and 45 sm. Experiences were pawned in quadruple frequency with the area of an allotment 10 M2. 60-65 day sprouts were planted under film tunnel shelters on February 11-12. Film shelters were kept over plants till the end of March. Seed plants were grown up without formation. Experiences were accompanied by phonological control, calculation of leaves quantity, an establishment of average weight of a head, definition of quantity of the plants, which have given seeds, the account of a crop of seeds from a bush and of a unit of area. It has been defined that reception of seeds of a cauliflower at cultivation under film tunnels is quite possible in Uzbekistan. It is established that with representation of the big growing space the plants form more leaves, earlier and larger form the heads, develop the testicles acceleration and increase their seed efficiency. It has been revealed that the more plants thickness is, the bigger share of them does not give seeds. In this connection the greatest crop of seeds has been received at growing space 70x45 sm at which 31.7 thousand Pl/ha had been planted. It is proved that in the conditions of Uzbekistan it is possible to cultivate high-grade seeds of cauliflower with productivity of 127-147 kg/ha.
文摘In the present study,self-propelled cabbage/cauliflower harvester was designed,developed and evaluated.The machine consisted of different components like engine,frame,shearing(cutting)unit and power transmission unit.The power transmission unit consisted of main clutch,shearing blade operating clutch,belt drive unit,chain and sprocket drive,universal joint and cutter blade assembly.The main working principle of harvester is based on shearing of crop stem against high-speed rotating blade.The power from the engine is transmitted by belt-pulley drive unit to transmission shaft on which chain and sprocket is mounted on one side and then power is transmitted to shearing blade coupling with the help of a stationary pulley and fixed socket.Average mean head diameter of the selected cabbage and cauliflower was 89.5±15.24 mm and 107.5±15.24 mm,respectively.Average mean stem(plant)diameter of the selected cabbage and cauliflower was 18±4.85 mm and 21.5±3.08 mm,respectively.The shearing force increased with increase in diameter of stem.The optimum performance of the machine was achieved when it was operated at 1.5 km/h forward speed and the shearing blade moving at speed of 147 rpm.The mean field capacity for developed prototype was observed as 0.063 ha/h and 0.053 in case of cabbage and cauliflower,respectively with field efficiency of 91.97 and 90.48%.The average head damage was negligible(0.15%)for both the crops.The average untrimmed percentage with developed harvester was 3.2 and 3.0%in case of cabbage and cauliflower crop,respectively.The developed machine helps to increase the field capacity in cabbage/cauliflower harvesting due to 7-times more capacity and 50%cheaper compared to traditional method of cabbage/cauliflower harvesting.At the operating condition of forward speed(1.5 km/h)and shearing blade speed(147 rpm),the machine could harvest 0.5 ha of cabbage and 0.42 ha of cauliflower farm per day of 8-h.This same task would have required between 15 labour per day if entirely done manually.
基金supported by the Technology Development Programme of Department of Science and Technology,Government of India(DST/TDT/TDP-22/2022)。
文摘This research explored a novel multimodal approach for disease management in cauliflower crops.With the rising challenges in sustainable agriculture,the research focused on a patch spraying method to control disease and reduce crop losses and environmental impact.For non-destructive disease assessment,a spectral sensor was used to collect spectral information from diseased and healthy cauliflower parts.The spectral data sets were analyzed using decision tree and support vector machine(SVM)algorithms to identify the most accurate model for distinguishing diseased and healthy plants.The chosen model was integrated with a low-volume sprayer(50-150 L·ha^(-1)),equipped with an electronic control unit for targeted spraying based on sensor-detected regions.The decision tree model achieved 89.9% testing accuracy,while the SVM model achieved 96.7% accuracy using hyperparameters:cost of 10.0 and tolerance of 0.001.The research successfully demonstrated the integration of spectral sensors,machine learning,and targeted spraying technology for precise input application.Additionally,the optimized sprayer achieved a 72.5% reduction in chemical usage and a significant time-saving of 21.0% compared to a standard sprayer for black rot-infested crops.These findings highlight the potential efficiency and resource conservation benefits of innovative sprayer technology in precision agriculture and disease management.
文摘I. INTRODUCTION Cauliflower mosaic virus (CaMV)is a double stranded DNA virus. It attracted attention in the pasf few years because of its special replication process and its possible use as a vector in plant genetic engineering. For a further study it is important to get the infectious clone of the CaMV genome at first. Although several infectious clones have been
基金supported by grants from the National Key Research and Development Program of China(Grant No.2022YFF1003000)the National Natural Science Foundation of China(Grant Nos.32472753 and 32402584)+1 种基金Basic Scientific Research of the Central Public Welfare Research Institutes(Grant No.1610232023013)the China Tobacco Genome Project(Grant No.110202103012).
文摘Cauliflower(Brassica oleracea var.botrytis L.),a cruciferous vegetable belonging to the Brassicaceae family,is a vital component of global vegetable production systems.Renowned for its high nutritional value,which includes substantial levels of protein,lipids,carbohydrates,dietary fiber,vitamins,and minerals,it provides significant health benefits.Taste components,particularly the aroma,influence the culinary and consumption experience,thereby affecting human food preferences(Lu and Zhu 2022).Current breeding initiatives prioritize the development of novel cultivars with enhanced organoleptic traits while maintaining yield stability,addressing the growing demand for premium horticultural commodities in global fresh produce markets.
基金partly funded by the project of technology innovation ability from Beijing Academy of Agriculture and Forestry Sciences (Grant Nos. KJCX20200401, KJCX20200205 and KJCX20200113)the Natural Science Foundation of China (Grant No. 31972401)
文摘In this study,we used the modified CRISPR/Cas9 system to produce targeted point mutations in cauliflower.Acetolactate synthase(ALS)and Centromere-specific histone H3 variant(CENH3)genes were selected as the base-editing targets and hypocotyls of cauliflower were used as explants.For ALS gene,a C-to-T conversion in the Pro182 codon(CCT)can alter the encoded amino acid,likely resulting in herbicide resistance,and a C-to-T mutation in the Leu133 codon(CTT)in the CENH3 gene may produce a haploid inducer.Results indicated that the transformation efficiency was 1.8%–4.5%and the mutation efficiencies for the ALS and CENH3 genes were approximately 22%and 87%,respectively.The ALS mutant cauliflower showed strong herbicide resistance,with possible immediate implications for broadleaf weed control in cauliflower fields.
文摘Investigation was made to confirm the stability of drought and salt stress tolerance in cauliflower (Brassica oleracea var.botrytis) mutants after regeneration and micropropagation. The N-nitroso-N-ethyleurea (NEU) and N-nitroso-N-methylurea (NMU) induced mutants of cauliflower were created and screened for drought and salt stress tolerance. The highly tolerant mutants were selected, regenerated by tissue culture techniques, screened again for drought and salt tolerance under in-vitro and in-vivo conditions, correlated the response of in-vitro and in-vivo plants within a clone. Free proline levels in clones were correlated with stress tolerance. Results confirmed the persistence of mutations in clones with enhanced resistance levels to stresses over control plants. The regenerated in-vitro and in-vivo plants within a clone showed a positive significant correlation for drought (R2 = 0.663) and salt (R2 = 0.647) resistance that confirms the stability of mutation in clones after generations. Proline showed a positive and significant correlation with drought (R2 = 0.524) and salt (R2 = 0.786) tolerance. Conclusively, drought and salt resistance can be successfully enhanced in cauliflower by chemical mutagenesis. Further molecular analysis is recommended to study these mutants.
文摘<strong>Introduction:</strong> Buschke Lowenstein Tumor (BLT) is a tumor, belonging to the group of verrucous carcinomas. It is endowed with an unpredictable potential of recurrence and degeneration. The aim of the work is to present the anatomical and therapeutic characteristics of BLT at the University Hospital of National Reference of N’Djamena in Chad. <strong>Patients and Method:</strong> Prospective descriptive study of 13 patients with BLT, from May 2009 to April 2019 in the department of urology was included. The patients more than 15, carriers of TBL located at: external genitalia, perineum and anal margin whose clinical, paraclinical assessment allowed the diagnosis of BLT, management and follow-up at the said service. Respect for the patient’s identity and consent, the patient’s consent is obtained and the anonymity of the photographs is observed. <strong>Result:</strong> A total of 13 patients had BLT, 11 males and 2 females. The average age of the patients was 32.5 years. The lesions were cauliflower-like and located on the vulva (n = 2), the penis, the perineum, the anorectal region in men (n = 11). Serology was positive for: HIV (n = 3), chlamydia (n = 4). The management was surgical by cauterization and excision. Cystoscopy and rectoscope were normal. The cure was obtained in all patients. Two patients had a recurrence at 5 months requiring a second successful cure. <strong>Conclusion:</strong> BLT is a frequent pathology in the urology department of the University Hospital of National Reference of N’Djamena in Chad. The surgical treatment by “cauterization exeresis” gives a better treatment.
