Objective: To study the blood pressure lowering effect of Cassytha filiformis extract in animal models of hypertension and its correlation with the antioxidant activity.Methods: Male Sprague–Dawley rats were divided ...Objective: To study the blood pressure lowering effect of Cassytha filiformis extract in animal models of hypertension and its correlation with the antioxidant activity.Methods: Male Sprague–Dawley rats were divided into two groups: endocrine hypertension(HTN group) that received a combination of prednisone and salt for two weeks and oxidative stress-associated hypertension(HTN-OS group) that received additional induction of L-Nitro Arginine Methyl Esther(L-NAME) for two days. Each group was subdivided into 4 and treated intravenously with the extract 5; 10; and 20 mg/kg, and vehicle control. The systolic blood pressure(SBP), diastolic blood pressure(DBP), and mean arterial pressure(MAP) were recorded. The blood was taken before and at the end of recording for the measurement of serum concentration of nitric oxide(NO). The changes of blood pressure were analyzed by two-way ANOVA while its correlation with NO concentration was analyzed by Pearson's Correlation.Results: The study showed a significant antihypertensive effect of the extract as compared with control group(P < 0.05) in both hypertensive models. Extract in the dose of 5 mg/kg showed the best blood pressure lowering effect. However, the correlation analysis did not show an association between NO increase and blood pressure lowering effect(P > 0.05).Conclusions: The study concludes that C. filiformis extract in the dose of 5 mg/kg exhibits the best blood pressure lowering effect in both animal models. Antihypertensive activity of the extract is not correlated with its antioxidant effect.展开更多
Cassytha filiformis L,belonging to family Lauraceae,is widely distributed in subtropical and tropical regions.Cassytha filiformis L is a traditional plant medicine in China,it has proved that the plant showed anti-par...Cassytha filiformis L,belonging to family Lauraceae,is widely distributed in subtropical and tropical regions.Cassytha filiformis L is a traditional plant medicine in China,it has proved that the plant showed anti-parasite,anti-tumor,anti-diabetic and anti-platelet aggregation activities,so far,the main pharmacological active components are alkaloids.In this paper,the chemical constituents and pharmacological effects of Cassytha filiformis L were reviewed by referring to databases such as CNKI,Vip and Pubmed,so as to lay a foundation for its development and utilization.展开更多
Objective:To determine the phytochemicals,total saponin content and types of saponin present in leaf extracts of Cassytha filiformis.Methods:The leaves were extracted with n-hexane and methanol.The methanol extract wa...Objective:To determine the phytochemicals,total saponin content and types of saponin present in leaf extracts of Cassytha filiformis.Methods:The leaves were extracted with n-hexane and methanol.The methanol extract was fractionated.The total saponin content of the butanol fraction was determined by colorimetry via a UV spectrophotometer and ginsenoside was used as the standard.Measurements were carried out at 550 nm.The butanol fraction was subjected to gas chromatography-mass spectrometer analysis.Results:All screened phytochemicals were absent in the n-hexane extract while saponins,steroids,tannins and glycosides were present in the methanol extract.Flavonoids and alkaloids were absent.The total saponin content of the methanol extract is 73.47μg ginsenoside Rb1 equivalent/g extract.The chromatography-mass spectrometer analysis gave eicosanoic acid,methyl ester as the most abundant compound and the steroidal saponin,cholestan-7-one and cyclic 1,2-ethanedienyl acetal as the most abundant saponin in the butanol fraction.Conclusions:The leaves of Cassytha filiformis are rich in steroidal saponins.展开更多
基金financially supported by Ministry of National Education and Culture, The Republic of Indonesia under the scheme of Fundamental Research [grant number 02/UN.16/PLFD/2013]
文摘Objective: To study the blood pressure lowering effect of Cassytha filiformis extract in animal models of hypertension and its correlation with the antioxidant activity.Methods: Male Sprague–Dawley rats were divided into two groups: endocrine hypertension(HTN group) that received a combination of prednisone and salt for two weeks and oxidative stress-associated hypertension(HTN-OS group) that received additional induction of L-Nitro Arginine Methyl Esther(L-NAME) for two days. Each group was subdivided into 4 and treated intravenously with the extract 5; 10; and 20 mg/kg, and vehicle control. The systolic blood pressure(SBP), diastolic blood pressure(DBP), and mean arterial pressure(MAP) were recorded. The blood was taken before and at the end of recording for the measurement of serum concentration of nitric oxide(NO). The changes of blood pressure were analyzed by two-way ANOVA while its correlation with NO concentration was analyzed by Pearson's Correlation.Results: The study showed a significant antihypertensive effect of the extract as compared with control group(P < 0.05) in both hypertensive models. Extract in the dose of 5 mg/kg showed the best blood pressure lowering effect. However, the correlation analysis did not show an association between NO increase and blood pressure lowering effect(P > 0.05).Conclusions: The study concludes that C. filiformis extract in the dose of 5 mg/kg exhibits the best blood pressure lowering effect in both animal models. Antihypertensive activity of the extract is not correlated with its antioxidant effect.
基金National Natural Science Foundation of China(No.82060778No.81760628)。
文摘Cassytha filiformis L,belonging to family Lauraceae,is widely distributed in subtropical and tropical regions.Cassytha filiformis L is a traditional plant medicine in China,it has proved that the plant showed anti-parasite,anti-tumor,anti-diabetic and anti-platelet aggregation activities,so far,the main pharmacological active components are alkaloids.In this paper,the chemical constituents and pharmacological effects of Cassytha filiformis L were reviewed by referring to databases such as CNKI,Vip and Pubmed,so as to lay a foundation for its development and utilization.
文摘Objective:To determine the phytochemicals,total saponin content and types of saponin present in leaf extracts of Cassytha filiformis.Methods:The leaves were extracted with n-hexane and methanol.The methanol extract was fractionated.The total saponin content of the butanol fraction was determined by colorimetry via a UV spectrophotometer and ginsenoside was used as the standard.Measurements were carried out at 550 nm.The butanol fraction was subjected to gas chromatography-mass spectrometer analysis.Results:All screened phytochemicals were absent in the n-hexane extract while saponins,steroids,tannins and glycosides were present in the methanol extract.Flavonoids and alkaloids were absent.The total saponin content of the methanol extract is 73.47μg ginsenoside Rb1 equivalent/g extract.The chromatography-mass spectrometer analysis gave eicosanoic acid,methyl ester as the most abundant compound and the steroidal saponin,cholestan-7-one and cyclic 1,2-ethanedienyl acetal as the most abundant saponin in the butanol fraction.Conclusions:The leaves of Cassytha filiformis are rich in steroidal saponins.
