Rapid and ultrasensitive detection of pathogen-associated biomarkers is vital for the early diagnosis and therapy of bacterial infections.Herein,we developed a close-packed and ordered Au@AgPt array coupled with a cas...Rapid and ultrasensitive detection of pathogen-associated biomarkers is vital for the early diagnosis and therapy of bacterial infections.Herein,we developed a close-packed and ordered Au@AgPt array coupled with a cascade triggering strategy for surface-enhanced Raman scattering(SERS)and colorimetric identification of the Staphylococcus aureus biomarker micrococcal nuclease(MNase)in serum samples.The trimetallic Au@AgPt nanozymes can catalyze the oxidation of 3,3’,5,5’-tetramethylbenzidine(TMB)molecules to SERS-enhanced oxidized TMB(oxTMB),accompanied by the color change from colorless to blue.In the presence of S.aureus,the secreted MNase preferentially cut the nucleobase AT-rich regions of DNA sequences on magnetic beads(MBs)to release alkaline phosphatase(ALP),which subsequently mediated the oxTMB reduction for inducing the colorimetric/SERS signal fade away.Using this“on-to-off”triggering strategy,the target S.aureus can be recorded in a wide linear range with a limit of detection of 38 CFU/mL in the colorimetric mode and 6 CFU/mL in the SERS mode.Meanwhile,the MNase-mediated strategy characterized by high specificity and sensitivity successfully discriminated between patients with sepsis(n=7)and healthy participants(n=3),as well as monitored the prognostic progression of the disease(n=2).Overall,benefiting from highly active and dense“hot spot”substrate,MNase-mediated cascade response strategy,and colorimetric/SERS dual-signal output,this methodology will offer a promising avenue for the early diagnosis of S.aureus infection.展开更多
Aiming to translate the success of blood detection technologies reported in numerous research papers into future development ideas for tumor interstitial fluid(TIF)detection,this review systematically summarizes the l...Aiming to translate the success of blood detection technologies reported in numerous research papers into future development ideas for tumor interstitial fluid(TIF)detection,this review systematically summarizes the latest advancements in electron-transfer and electrochemical signal-readout based sensing of biomarkers from the perspective of multiphase interface reaction flow transfer and control.The review covers electrochemical biosensing with immobilized sensing elements on electrode surfaces,transient or steady-state electrochemical signal readout based on interfacial collision electrochemistry,and homogeneous electrochemical sensors with constrained coupling of electron transfer cascades.We discuss strategies to enhance sensing reliability from three aspects:Avoiding target information loss,signal amplification,and background interference removal.In particular,the readout modes based on accumulated signals after transfer-response-conversion-amplification processes and dynamic feature extraction during the process are discussed and analyzed in detail.Furthermore,this review explores the translation of these blood-based diagnostics to TIF analysis,providing insights into the potential diagnostic and prognostic utility of TIF-based technologies.展开更多
This paper considers the modeling and convergence of hyper-networked evolutionary games （HNEGs）. In an HNEG the network graph is a hypergraph, which allows the fundamental network game to be a multi-player one. Usin...This paper considers the modeling and convergence of hyper-networked evolutionary games （HNEGs）. In an HNEG the network graph is a hypergraph, which allows the fundamental network game to be a multi-player one. Using semi-tensor product of matrices and the fundamental evolutionary equation, the dynamics of an HNEG is obtained and we extend the results about the networked evolutionary games to show whether an HNEG is potential and how to calculate the potential. Then we propose a new strategy updating rule, called the cascading myopic best response adjustment rule （MBRAR）, and prove that under the cascading MBRAR the strategies of an HNEG will converge to a pure Nash equilibrium. An example is presented and discussed in detail to demonstrate the theoretical and numerical results.展开更多
基金supported by the National Natural Science Foundation of China(Grant Nos.:82373833,22177039,and 82304438)the National Key Research and Development Program of China(Grant No.:2021YFC2300400)Guangdong Basic and Applied Basic Research Foundation,China(Grant Nos.:2024A1515012204,2022A1515010300,and 2022A1515110618).
文摘Rapid and ultrasensitive detection of pathogen-associated biomarkers is vital for the early diagnosis and therapy of bacterial infections.Herein,we developed a close-packed and ordered Au@AgPt array coupled with a cascade triggering strategy for surface-enhanced Raman scattering(SERS)and colorimetric identification of the Staphylococcus aureus biomarker micrococcal nuclease(MNase)in serum samples.The trimetallic Au@AgPt nanozymes can catalyze the oxidation of 3,3’,5,5’-tetramethylbenzidine(TMB)molecules to SERS-enhanced oxidized TMB(oxTMB),accompanied by the color change from colorless to blue.In the presence of S.aureus,the secreted MNase preferentially cut the nucleobase AT-rich regions of DNA sequences on magnetic beads(MBs)to release alkaline phosphatase(ALP),which subsequently mediated the oxTMB reduction for inducing the colorimetric/SERS signal fade away.Using this“on-to-off”triggering strategy,the target S.aureus can be recorded in a wide linear range with a limit of detection of 38 CFU/mL in the colorimetric mode and 6 CFU/mL in the SERS mode.Meanwhile,the MNase-mediated strategy characterized by high specificity and sensitivity successfully discriminated between patients with sepsis(n=7)and healthy participants(n=3),as well as monitored the prognostic progression of the disease(n=2).Overall,benefiting from highly active and dense“hot spot”substrate,MNase-mediated cascade response strategy,and colorimetric/SERS dual-signal output,this methodology will offer a promising avenue for the early diagnosis of S.aureus infection.
基金supported in part by the National Natural Science Foundation of China(Nos.22475028,22175027)the Natural Science Foundation of Chongqing(Nos.cstc2021jcyj-msxmX0178,cstc2021jcyj-cxttX0002)project supported by Graduate Research and Innovation Foundation of Chongqing,China(No.CYB23071).
文摘Aiming to translate the success of blood detection technologies reported in numerous research papers into future development ideas for tumor interstitial fluid(TIF)detection,this review systematically summarizes the latest advancements in electron-transfer and electrochemical signal-readout based sensing of biomarkers from the perspective of multiphase interface reaction flow transfer and control.The review covers electrochemical biosensing with immobilized sensing elements on electrode surfaces,transient or steady-state electrochemical signal readout based on interfacial collision electrochemistry,and homogeneous electrochemical sensors with constrained coupling of electron transfer cascades.We discuss strategies to enhance sensing reliability from three aspects:Avoiding target information loss,signal amplification,and background interference removal.In particular,the readout modes based on accumulated signals after transfer-response-conversion-amplification processes and dynamic feature extraction during the process are discussed and analyzed in detail.Furthermore,this review explores the translation of these blood-based diagnostics to TIF analysis,providing insights into the potential diagnostic and prognostic utility of TIF-based technologies.
基金supported partly by National Natural Science Foundation of China(Nos.61074114 and 61273013)
文摘This paper considers the modeling and convergence of hyper-networked evolutionary games （HNEGs）. In an HNEG the network graph is a hypergraph, which allows the fundamental network game to be a multi-player one. Using semi-tensor product of matrices and the fundamental evolutionary equation, the dynamics of an HNEG is obtained and we extend the results about the networked evolutionary games to show whether an HNEG is potential and how to calculate the potential. Then we propose a new strategy updating rule, called the cascading myopic best response adjustment rule （MBRAR）, and prove that under the cascading MBRAR the strategies of an HNEG will converge to a pure Nash equilibrium. An example is presented and discussed in detail to demonstrate the theoretical and numerical results.
