The identification and quality evaluation of Flos carthami were studied using tunable liquid spectral imaging instrument, to discuss the application range and advantages of spectral imaging technology in Chinese medic...The identification and quality evaluation of Flos carthami were studied using tunable liquid spectral imaging instrument, to discuss the application range and advantages of spectral imaging technology in Chinese medicine identification and quality control field. The Flos carthami was indentified by extracting the normalized characteristic spectral curves of Flos carthami, Crocus sativus and Dendranthema morifolium, which were standard samples supplied by National Institute for Drug Control. The qualities of Flos carthamies collecting from different pharmacies were evaluated by extracting their normalized characteristic spectral curves. The imaging spectrum testing system was designed independently. The spectral resolution was 5 nm, and the spectral range was from 400 nm to 680 nm. The results showed that the normalized characteristic spectral curve of Flos carthami was significantly different from those of Crocus sativus’ and Dendranthema morifolium’s, and the fluorescence intensity of Flos carthami from different commercial sources were different. Spectral imaging technology could be used to identify and evaluate Flos carthami, and operation method was rapid, convenient and non-destructive.展开更多
Objective Xuebijing Injection(XBJI)is mainly used for treating sepsis in China,and even COVID-19 recently.This study aimed to clarify the molecular mechanism(s)and identify the potential“common culprit(s)”for XBJI-c...Objective Xuebijing Injection(XBJI)is mainly used for treating sepsis in China,and even COVID-19 recently.This study aimed to clarify the molecular mechanism(s)and identify the potential“common culprit(s)”for XBJI-caused immediate hypersensitivity reaction(IHR)which is the main type of its adverse reactions.Methods Antiserum against XBJI was prepared by intraperitoneal immunization in combination with aluminum adjuvant for five weeks.Antagonistic experiments were performed by using several antagonists against different mediators in Evans Blue leakage model.Propranolol-pretreated mice were used to determine the capacity of XBJI to trigger systemic IHR.Serum total IgE(tIgE)and mouse mast cell protease 1(MCPT-1)levels,complement activation,and the levels of supernatant inflammatory mediators were determined by ELISAs.Lipopolysaccharide(LPS)-activated RAW264.7 macrophages were used for evaluating the anti-inflammatory activity of XBJI,while human mast cells(LAD2)were used for assessing the effect of XBJI on mast cell degranulation.Results Continuous treatment(i.p.)with XBJI along with aluminum adjuvant did not elevate the levels of serum tIgE and MCPT-1.In vitro,XBJI could not directly cause the degranulation of LAD2 cells.It induced a robust Evans Blue leakage after the first injection in mouse paw.Mechanism study demonstrated that antagonists for histamine H1/H2 receptors and complement C3a receptor counteracted XBJI-induced IHR.XBJI also directly activated complement C3 in human serum.Through screening five herbs of XBJI and the constituents,only safflower yellow(SY)in Carthami Flos was able to induce IHR.The discolored-XBJI not only did not induce IHR locally and systemically,but also could suppressing the production of proinflammatory mediators in LPS-activated RAW264.7 macrophages.Conclusion XBJI failed to induce immune IHR,but potently triggered non-immune IHR through direct activating complement C3 to provoke histamine release.SY in Carthami Flos was the underlying“common culprit”responsible for XBJI-caused IHR.The anti-inflammatory action of XBJI can be retained after decolorization.Our study provides a scientific basis for not only preventing and treating XBJI-caused IHR clinically,but also improving its production process.展开更多
文摘The identification and quality evaluation of Flos carthami were studied using tunable liquid spectral imaging instrument, to discuss the application range and advantages of spectral imaging technology in Chinese medicine identification and quality control field. The Flos carthami was indentified by extracting the normalized characteristic spectral curves of Flos carthami, Crocus sativus and Dendranthema morifolium, which were standard samples supplied by National Institute for Drug Control. The qualities of Flos carthamies collecting from different pharmacies were evaluated by extracting their normalized characteristic spectral curves. The imaging spectrum testing system was designed independently. The spectral resolution was 5 nm, and the spectral range was from 400 nm to 680 nm. The results showed that the normalized characteristic spectral curve of Flos carthami was significantly different from those of Crocus sativus’ and Dendranthema morifolium’s, and the fluorescence intensity of Flos carthami from different commercial sources were different. Spectral imaging technology could be used to identify and evaluate Flos carthami, and operation method was rapid, convenient and non-destructive.
基金supported by CAMS Innovation Fund for Medical Sciences(CIFMS)(No.2021-I2M-1-028 and No.2021-I2M-1-031)National Natural Science Foundation of China(No.82074091)Beijing Natural Science Foundation(No.M21014).
文摘Objective Xuebijing Injection(XBJI)is mainly used for treating sepsis in China,and even COVID-19 recently.This study aimed to clarify the molecular mechanism(s)and identify the potential“common culprit(s)”for XBJI-caused immediate hypersensitivity reaction(IHR)which is the main type of its adverse reactions.Methods Antiserum against XBJI was prepared by intraperitoneal immunization in combination with aluminum adjuvant for five weeks.Antagonistic experiments were performed by using several antagonists against different mediators in Evans Blue leakage model.Propranolol-pretreated mice were used to determine the capacity of XBJI to trigger systemic IHR.Serum total IgE(tIgE)and mouse mast cell protease 1(MCPT-1)levels,complement activation,and the levels of supernatant inflammatory mediators were determined by ELISAs.Lipopolysaccharide(LPS)-activated RAW264.7 macrophages were used for evaluating the anti-inflammatory activity of XBJI,while human mast cells(LAD2)were used for assessing the effect of XBJI on mast cell degranulation.Results Continuous treatment(i.p.)with XBJI along with aluminum adjuvant did not elevate the levels of serum tIgE and MCPT-1.In vitro,XBJI could not directly cause the degranulation of LAD2 cells.It induced a robust Evans Blue leakage after the first injection in mouse paw.Mechanism study demonstrated that antagonists for histamine H1/H2 receptors and complement C3a receptor counteracted XBJI-induced IHR.XBJI also directly activated complement C3 in human serum.Through screening five herbs of XBJI and the constituents,only safflower yellow(SY)in Carthami Flos was able to induce IHR.The discolored-XBJI not only did not induce IHR locally and systemically,but also could suppressing the production of proinflammatory mediators in LPS-activated RAW264.7 macrophages.Conclusion XBJI failed to induce immune IHR,but potently triggered non-immune IHR through direct activating complement C3 to provoke histamine release.SY in Carthami Flos was the underlying“common culprit”responsible for XBJI-caused IHR.The anti-inflammatory action of XBJI can be retained after decolorization.Our study provides a scientific basis for not only preventing and treating XBJI-caused IHR clinically,but also improving its production process.
文摘目的:基于网络药理学分析丹参-红花药对防治肺动脉高压(pulmonary hypertension,PH)的作用机制。方法:在中药系统药理学数据库与分析平台检索丹参、红花的化学成分及相关靶点。通过GeneCards、OMIM、DrugBank数据库获取PH相关靶点,将其导入STRING数据库获得蛋白互作关系,并利用Cytoscape-v3.8.2软件进行拓扑分析,筛选PH核心靶点。将核心靶点与丹参、红花的有效成分靶蛋白取交集,获取丹参-红花药对防治PH的潜在靶点。将潜在靶点导入Rx644.0.2软件进行基因本体论(Gene Ontology,GO)富集分析和京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)信号通路富集分析。将丹参、红花的有效成分和潜在靶点导入Cytoscape-v 3.8.2软件,构建“成分-靶点”网络,拓扑分析筛选关键靶点及相应有效成分,并运用AutoDock Vina软件进行分子对接。结果:丹参-红花药对共有62种有效成分及212个靶蛋白。通过GeneCards、OMIM、DrugBank数据库筛选出134个PH相关蛋白,与化学成分相关靶点取交集获得46个共同靶点。GO功能富集分析共获得2316个功能项,其中生物学过程2162项、细胞组分29项、分子功能125项;KEGG富集分析共获得166条信号通路。“成分-靶点”网络分析显示,RELA、TP53、JUN、TNF等为关键靶点,槲皮素、木犀草素、黄芩素、山柰酚、丹参酮ⅡA和隐丹参酮等为核心成分。分子对接显示,核心成分与关键靶点对接良好。结论:丹参-红花药对可能通过槲皮素、木犀草素、黄芩素等多种有效成分作用于TNF、RELA、TP53等核心蛋白调控PI3K-AKT、Th17细胞分化、TNF等通路,进而调控氧化应激、细胞增殖、蛋白磷酸化等生物过程发挥防治PH的作用。
