The identification and quality evaluation of Flos carthami were studied using tunable liquid spectral imaging instrument, to discuss the application range and advantages of spectral imaging technology in Chinese medic...The identification and quality evaluation of Flos carthami were studied using tunable liquid spectral imaging instrument, to discuss the application range and advantages of spectral imaging technology in Chinese medicine identification and quality control field. The Flos carthami was indentified by extracting the normalized characteristic spectral curves of Flos carthami, Crocus sativus and Dendranthema morifolium, which were standard samples supplied by National Institute for Drug Control. The qualities of Flos carthamies collecting from different pharmacies were evaluated by extracting their normalized characteristic spectral curves. The imaging spectrum testing system was designed independently. The spectral resolution was 5 nm, and the spectral range was from 400 nm to 680 nm. The results showed that the normalized characteristic spectral curve of Flos carthami was significantly different from those of Crocus sativus’ and Dendranthema morifolium’s, and the fluorescence intensity of Flos carthami from different commercial sources were different. Spectral imaging technology could be used to identify and evaluate Flos carthami, and operation method was rapid, convenient and non-destructive.展开更多
文摘The identification and quality evaluation of Flos carthami were studied using tunable liquid spectral imaging instrument, to discuss the application range and advantages of spectral imaging technology in Chinese medicine identification and quality control field. The Flos carthami was indentified by extracting the normalized characteristic spectral curves of Flos carthami, Crocus sativus and Dendranthema morifolium, which were standard samples supplied by National Institute for Drug Control. The qualities of Flos carthamies collecting from different pharmacies were evaluated by extracting their normalized characteristic spectral curves. The imaging spectrum testing system was designed independently. The spectral resolution was 5 nm, and the spectral range was from 400 nm to 680 nm. The results showed that the normalized characteristic spectral curve of Flos carthami was significantly different from those of Crocus sativus’ and Dendranthema morifolium’s, and the fluorescence intensity of Flos carthami from different commercial sources were different. Spectral imaging technology could be used to identify and evaluate Flos carthami, and operation method was rapid, convenient and non-destructive.
文摘目的:基于网络药理学分析丹参-红花药对防治肺动脉高压(pulmonary hypertension,PH)的作用机制。方法:在中药系统药理学数据库与分析平台检索丹参、红花的化学成分及相关靶点。通过GeneCards、OMIM、DrugBank数据库获取PH相关靶点,将其导入STRING数据库获得蛋白互作关系,并利用Cytoscape-v3.8.2软件进行拓扑分析,筛选PH核心靶点。将核心靶点与丹参、红花的有效成分靶蛋白取交集,获取丹参-红花药对防治PH的潜在靶点。将潜在靶点导入Rx644.0.2软件进行基因本体论(Gene Ontology,GO)富集分析和京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)信号通路富集分析。将丹参、红花的有效成分和潜在靶点导入Cytoscape-v 3.8.2软件,构建“成分-靶点”网络,拓扑分析筛选关键靶点及相应有效成分,并运用AutoDock Vina软件进行分子对接。结果:丹参-红花药对共有62种有效成分及212个靶蛋白。通过GeneCards、OMIM、DrugBank数据库筛选出134个PH相关蛋白,与化学成分相关靶点取交集获得46个共同靶点。GO功能富集分析共获得2316个功能项,其中生物学过程2162项、细胞组分29项、分子功能125项;KEGG富集分析共获得166条信号通路。“成分-靶点”网络分析显示,RELA、TP53、JUN、TNF等为关键靶点,槲皮素、木犀草素、黄芩素、山柰酚、丹参酮ⅡA和隐丹参酮等为核心成分。分子对接显示,核心成分与关键靶点对接良好。结论:丹参-红花药对可能通过槲皮素、木犀草素、黄芩素等多种有效成分作用于TNF、RELA、TP53等核心蛋白调控PI3K-AKT、Th17细胞分化、TNF等通路,进而调控氧化应激、细胞增殖、蛋白磷酸化等生物过程发挥防治PH的作用。
