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Microwave-Assisted Rapid Extracellular Biosynthesis of Silver Nanoparticles Using Carom Seed (Trachyspermum copticum) Extract and in Vitro Studies
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作者 Deshpande Raghunandan Prashant Arunkumar Borgaonkar +5 位作者 Basawaraj Bendegumble Mahesh Dhondojirao Bedre Mantripragada Bhagawanraju Manjunath Sooganna Yalagatti Do Sung Huh Venkataramana Abbaraju 《American Journal of Analytical Chemistry》 2011年第4期475-483,共9页
Microwave-assisted rapid extracellular biosynthesis of silver nanoparticles was carried out by using carom seed (Trachyspermum copticum) extract as the reducing agent. The reaction mixture containing AgNO3 and carom s... Microwave-assisted rapid extracellular biosynthesis of silver nanoparticles was carried out by using carom seed (Trachyspermum copticum) extract as the reducing agent. The reaction mixture containing AgNO3 and carom seed extract when exposed to microwave irradiation resulted in reducing silver ions to bio-functionalized silver nanoparticles of size 6- 50 nm. The AgNP were characterized by UV-vis spectroscopy (UV-vis), X-ray diffraction (XRD), energy dispersive X-ray analysis (EDAX), field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM) and atomic force microscopy (AFM). Themogravimetric analysis (TGA) and fourier transform infrared spectroscopy (FTIR) are used to understand the possible mechanism of biosynthesis. In this study, we have also investigated the antimicrobial and antioxidant activities of bio-functionalized AgNP. The antibacterial activity is investigated by measuring the zone of inhibition and antioxidant study is done using 1,1-diphenyl-2-picryl hydrazyl (DPPH) method. 展开更多
关键词 Microwave Silver Nanoparticles BIOSYNTHESIS carom SEED (Trachyspermum copticum) Antibacterial ACTIVITY Antioxidant ACTIVITY
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胡萝卜(DaucuscarotaL.)有丝分裂核型及减数分裂观察 被引量:6
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作者 张永兵 庄飞云 +1 位作者 赵志伟 陈劲枫 《上海农业学报》 CSCD 2005年第3期26-28,共3页
用对二氯苯(饱和)和放线菌酮(40×10-6g·L-1)的混合药剂对胡萝卜根尖进行预处理,通过压片获得了分散良好、形态清晰的有丝分裂中期染色体。核型分析结果表明,胡萝卜染色体平均长度为2.34μm,在4号染色体上发现一对随体。臂比&g... 用对二氯苯(饱和)和放线菌酮(40×10-6g·L-1)的混合药剂对胡萝卜根尖进行预处理,通过压片获得了分散良好、形态清晰的有丝分裂中期染色体。核型分析结果表明,胡萝卜染色体平均长度为2.34μm,在4号染色体上发现一对随体。臂比>2的染色体占22.2%,最长染色体与最短染色体的比值为2.17,核型类型为2B型,核型公式为2n=2x=18=8sm(2SAT)+10m。通过观察胡萝卜花粉母细胞的减数分裂过程,发现其行为正常,是同时型胞质分裂,中期I有9个二价体(9II),为典型的二倍体物种。 展开更多
关键词 胡萝卜 有丝分裂 核型 减数分裂
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