Salicylic acid(SA)is a hydrolysis product and an active form of aspirin,and SA is found in a range of fruits and other food products.For food and drug and analysis there is a strong desire to detect SA.Since SA is a v...Salicylic acid(SA)is a hydrolysis product and an active form of aspirin,and SA is found in a range of fruits and other food products.For food and drug and analysis there is a strong desire to detect SA.Since SA is a very small molecule,aptamers have advantages over antibodies for its detection.In this work,we used the libraryimmobilization capture-SELEX method to isolate aptamers for SA.After 17 rounds of selection,two main families of aptamers were isolated.The SA1 aptamer from family 1 has a K_(d)of 5.8μM from a thioflavin T(ThT)fluorescence assay and 26.7μM from isothermal titration calorimetry.The binding of other sequences was weaker compared to SA1.Based on mutation studies,the two conserved regions of SA1 were connected by two stems.Using ThT as a stain,a label-free fluorescent sensor was tested for the detection of SA with a detection limit of 2.2μM.A few similar molecules were tested including aspirin,and only p-hydroxybenzoic acid showed a weak binding,indicating the high specificity of the SA1 aptamer.Finally,the SA1 aptamer was also tested in tomato juice and a similar binding performance was achieved.展开更多
Rimantadine(RIM),as a commonly used antiviral drug,is often illegally used in the aquaculture industry due to its remarkable therapeutic effect and low price,resulting in the accumulation of RIM in the environment and...Rimantadine(RIM),as a commonly used antiviral drug,is often illegally used in the aquaculture industry due to its remarkable therapeutic effect and low price,resulting in the accumulation of RIM in the environment and eventually threatening human health.Therefore,the realization of specific detection and monitoring of RIM has become an important topic in food safety and other fields.In this work,we screened an aptamer RIM-17 with high specificity and affinity for RIM based on Capture-systematic evolution of ligands by exponential enrichment(Capture-SELEX)technology.The binding mechanism and key sites of RIM and RIM-17 was studied by molecular docking technology.According to the molecular binding site,the optimal aptamer RIM-172 with a length of 50-mer was obtained by truncation optimization strategy,and its affinity Kd value for RIM was 46.84±11.33 nM.Based on the optimized aptamer,a surface-enhanced Raman spectroscopy(SERS)aptasensor with Fe_(3)O_(4)@UIO-66@AuNS as the Raman report substrate was established for the detection of RIM.Under the optimal preparation conditions,the linear range of the SERS-based aptasensor to detect RIM was 0.5~25 ng/mL and the limit of detection(LOD)was 0.27 ng/mL.In the test of added standard recovery,the recovery rate was 96.00%~101.20%.Moreover,the aptasensor exhibited accepted stability and specificity.The discovery of RIM aptamer provides an effective way for the specific capture and detection of RIM,and helps to monitor the illegal abuse of RIM.展开更多
The design of advanced dual-mode aptasensors has emerged as a promising strategy for the reliable detection of contaminants,with nanomaterial-mediated signal output playing a pivotal role.Herein,we developed a novel m...The design of advanced dual-mode aptasensors has emerged as a promising strategy for the reliable detection of contaminants,with nanomaterial-mediated signal output playing a pivotal role.Herein,we developed a novel multifunctional nanocomposite material(FePOs@CeO_(2))-mediated colorimetric/electrochemical dual-mode aptasensor for the sensitive and specific detection of amoxicillin(AMO),a widely used semi-synthetic peni-cillin.Therein,FePOs@CeO_(2)relied on the catalytic ability of FePOs and the excellent electrochemical perfor-mance of cerium’s multivalent states(Ce^(3+)/Ce^(4+))to promote effective electron transfer at the electrode interface,thereby exhibiting excellent peroxidase(POD)like activity and conductive properties.Furthermore,an aptamer AMO-1 with specific binding affinity for AMO was obtained and its truncated derivative(AMO-1b)with enhanced binding affinity was obtained through molecular docking simulation and secondary structure analysis.The specific binding of this high-affinity aptamer to AMO caused FePOs@CeO_(2)to detach from the electrode surface,thereby triggering a change in the electrochemical signal.The collected FePOs@CeO_(2)solution induced a color change,thereby enabling the colorimetric detection function.The sensing platform demonstrated high sensitivity,specificity,and accuracy in the detection of AMO,with detection limits of 0.828μg/mL(colorimetric)and 7.24×10^(-5)μg/mL(electrochemical).In addition,the sensor exhibited good practicality in the detection of AMO in milk,with recovery rates of 97.49%-98.25%(colorimetric)and 92.24%-93.01%(electrochemical),respectively.The dual detection results could verify each other,which improved the accuracy and reliability of the results and provided a strategy for the design of a novel biosensing platform for veterinary drug residue detection.展开更多
基金Funding for this work was from the Natural Sciences and Engineering Research Council of Canada(NSERC).
文摘Salicylic acid(SA)is a hydrolysis product and an active form of aspirin,and SA is found in a range of fruits and other food products.For food and drug and analysis there is a strong desire to detect SA.Since SA is a very small molecule,aptamers have advantages over antibodies for its detection.In this work,we used the libraryimmobilization capture-SELEX method to isolate aptamers for SA.After 17 rounds of selection,two main families of aptamers were isolated.The SA1 aptamer from family 1 has a K_(d)of 5.8μM from a thioflavin T(ThT)fluorescence assay and 26.7μM from isothermal titration calorimetry.The binding of other sequences was weaker compared to SA1.Based on mutation studies,the two conserved regions of SA1 were connected by two stems.Using ThT as a stain,a label-free fluorescent sensor was tested for the detection of SA with a detection limit of 2.2μM.A few similar molecules were tested including aspirin,and only p-hydroxybenzoic acid showed a weak binding,indicating the high specificity of the SA1 aptamer.Finally,the SA1 aptamer was also tested in tomato juice and a similar binding performance was achieved.
基金supported by the National Key Research and Development Program of China(2022YFF110110306)National Natural Science Foundation of China(32272449)+1 种基金the Fundamental Research Funds for the Central Universities(JUSRP622025)Collaborative Appendix A.Supplementary data。
文摘Rimantadine(RIM),as a commonly used antiviral drug,is often illegally used in the aquaculture industry due to its remarkable therapeutic effect and low price,resulting in the accumulation of RIM in the environment and eventually threatening human health.Therefore,the realization of specific detection and monitoring of RIM has become an important topic in food safety and other fields.In this work,we screened an aptamer RIM-17 with high specificity and affinity for RIM based on Capture-systematic evolution of ligands by exponential enrichment(Capture-SELEX)technology.The binding mechanism and key sites of RIM and RIM-17 was studied by molecular docking technology.According to the molecular binding site,the optimal aptamer RIM-172 with a length of 50-mer was obtained by truncation optimization strategy,and its affinity Kd value for RIM was 46.84±11.33 nM.Based on the optimized aptamer,a surface-enhanced Raman spectroscopy(SERS)aptasensor with Fe_(3)O_(4)@UIO-66@AuNS as the Raman report substrate was established for the detection of RIM.Under the optimal preparation conditions,the linear range of the SERS-based aptasensor to detect RIM was 0.5~25 ng/mL and the limit of detection(LOD)was 0.27 ng/mL.In the test of added standard recovery,the recovery rate was 96.00%~101.20%.Moreover,the aptasensor exhibited accepted stability and specificity.The discovery of RIM aptamer provides an effective way for the specific capture and detection of RIM,and helps to monitor the illegal abuse of RIM.
基金supported by the National Natural Science Foundation of China(32272449)the Basic Research Program of Jiangsu(BK20240100)+1 种基金the Fundamental Research Funds for the Central Universities(JUSRP622025)Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province.
文摘The design of advanced dual-mode aptasensors has emerged as a promising strategy for the reliable detection of contaminants,with nanomaterial-mediated signal output playing a pivotal role.Herein,we developed a novel multifunctional nanocomposite material(FePOs@CeO_(2))-mediated colorimetric/electrochemical dual-mode aptasensor for the sensitive and specific detection of amoxicillin(AMO),a widely used semi-synthetic peni-cillin.Therein,FePOs@CeO_(2)relied on the catalytic ability of FePOs and the excellent electrochemical perfor-mance of cerium’s multivalent states(Ce^(3+)/Ce^(4+))to promote effective electron transfer at the electrode interface,thereby exhibiting excellent peroxidase(POD)like activity and conductive properties.Furthermore,an aptamer AMO-1 with specific binding affinity for AMO was obtained and its truncated derivative(AMO-1b)with enhanced binding affinity was obtained through molecular docking simulation and secondary structure analysis.The specific binding of this high-affinity aptamer to AMO caused FePOs@CeO_(2)to detach from the electrode surface,thereby triggering a change in the electrochemical signal.The collected FePOs@CeO_(2)solution induced a color change,thereby enabling the colorimetric detection function.The sensing platform demonstrated high sensitivity,specificity,and accuracy in the detection of AMO,with detection limits of 0.828μg/mL(colorimetric)and 7.24×10^(-5)μg/mL(electrochemical).In addition,the sensor exhibited good practicality in the detection of AMO in milk,with recovery rates of 97.49%-98.25%(colorimetric)and 92.24%-93.01%(electrochemical),respectively.The dual detection results could verify each other,which improved the accuracy and reliability of the results and provided a strategy for the design of a novel biosensing platform for veterinary drug residue detection.
