[Objective] This study aimed to screen out hot pepper germplasms highly resistant to Meloidogyne incognita, thereby providing resistant resources for hot pep- per breeding. [Method] Comprehensive analysis combining cl...[Objective] This study aimed to screen out hot pepper germplasms highly resistant to Meloidogyne incognita, thereby providing resistant resources for hot pep- per breeding. [Method] Comprehensive analysis combining cluster analysis and sub- ordinate function was conducted through determining related resistance indexes of 67 hot pepper germplasms 50 days after inoculated with M. incognita. [Result] The effects of M. incognita on related resistance indexes were significantly different am- ong the hot pepper germplasms. Egg index and gall index had abundant genetic variation with variation coefficients of 143.16% and 118.95%, respectively. Based on the gall indexes, cluster analysis of hot pepper germplasms was performed. The 67 hot pepper germplasms were divided into 4 groups （resistant, moderately resistant, susceptible and high susceptible）. The resistance intensity of the hot pepper germplasms were ranked according to the sum of subordinate function values of various resistance indexes. The total function values of Rela 2 and L506M were the largest （2.00）, indicating that these two germplasms were immune to M. incognita. The total function values of L287-2, L522-1M, L504M, L515-2, 13SM100-1, L512M, L292-1, L319, L316, L317, 13SM87-1 and Rela 5 were larger than 1.95, indicating that these germplasms were highly resistant to M. incognita. [Conclusion] This study could provide certain resistant resources for resistance breeding of hot pepper to M. incognita.展开更多
Pepper（Capsicum spp.） is an important vegetable crop in the world. Now the pepper in China contributes one-third of the world＇s peppers production. Genetic diversity of the pepper germplasm of China is expected int...Pepper（Capsicum spp.） is an important vegetable crop in the world. Now the pepper in China contributes one-third of the world＇s peppers production. Genetic diversity of the pepper germplasm of China is expected interesting to know. To explore the structure of genetic diversity in Chinese pepper germplasm resources and possible relationship with cultivar types or geographic origin, we sampled and compared 372 Gen Bank pepper accessions（local cultivars and landraces） from 31 provinces, autonomous regions and municipalities of China and 31 additional accessions from other countries. These accessions were genotyped using 28 simple sequence repeat（SSR） markers spanning the entire pepper genome. We then investigated the genetic structure of the sampled collection using model-based analysis in STRUCTURE v2.3.4 and examined genetic relationships by the unweighted pair-group method of mathematical averages（UPGMA） in MEGA. In addition to geographic origin, we evaluated eight plant and fruit traits. In total, 363 alleles were amplified using the 28 SSR primers. Gene diversity, polymorphism information content and heterozygosity of the 28 SSR loci were estimated as 0.09–0.92, 0.08–0.92 and 0.01–0.34, respectively. The UPGMA cluster analysis clearly distinguished Capsicum annuum L. from other cultivated pepper species. Population structure analysis of the 368 C. annuum accessions uncovered three genetic groups which also corresponded to distinct cultivar types with respect to the plant and fruit descriptors. The genetic structure was also related to the geographic origin of the landraces. Overall results indicate that genetic diversity of Chinese pepper landraces were structured by migration of genotypes followed by human selection for cultivar types in agreement with consumption modes and adaptation to the highly diversified agro-climatic conditions.展开更多
The present study was carried out in the form of a survey with the aim of listing and describing the technical itineraries of production, storage and conservation used in six departments of high production of chilli p...The present study was carried out in the form of a survey with the aim of listing and describing the technical itineraries of production, storage and conservation used in six departments of high production of chilli pepper in Benin. The survey was conducted on the basis of a questionnaire in the six departments of Benin: Ouémé, Plateau, Atlantic, Littoral, Zou and Collines. The survey collected information on the socio-economic characteristics of producers, the varieties of chilli pepper grown, and the current technical itineraries used for the production, storage and conservation of chilli pepper. On the other hand, it also gave an idea of the constraints that prevent the production of chilli pepper of good sanitary quality. The results of this survey show that three varieties of chilli peppers are the most produced and consumed in Benin. These are the Gbotakin variety (<i>Capsicum</i> <i>chinense</i>), the Afundja variety (<i>Capsicum</i> <i>annuum</i>) and the Danhomètakin variety (<i>Capsicum</i> <i>frutescens</i>). The technical itineraries related to the cultivation of these chilli peppers varieties are the same in the six study departments. Unfortunately, their contamination sometimes starts at the nursery stage, passing through the field where other contaminants are added and develop until harvest. After harvest, contamination can continue in storage and finally reach the consumer if proper measures are not taken. The inappropriate or inadequate use of phytosanitary products in the chilli pepper industry is also a source of illness for consumers of this vegetable fruit. Training of producers on the best technical itineraries for the production and storage of chilli peppers is essential to revitalize and develop this sector by improving the sanitary quality of the chilli peppers produced in Benin.展开更多
基金Supported by National Nonprofit Institute Research Grant of CATAS-TCGRI(1630032014019,1630032015003)Key Research&Development Project of Hainan Province(ZDYF2016225)Key Technology Research and Demonstration Project of Farmland Improvement of Hainan Province(HNGDpz2015)
文摘[Objective] This study aimed to screen out hot pepper germplasms highly resistant to Meloidogyne incognita, thereby providing resistant resources for hot pep- per breeding. [Method] Comprehensive analysis combining cluster analysis and sub- ordinate function was conducted through determining related resistance indexes of 67 hot pepper germplasms 50 days after inoculated with M. incognita. [Result] The effects of M. incognita on related resistance indexes were significantly different am- ong the hot pepper germplasms. Egg index and gall index had abundant genetic variation with variation coefficients of 143.16% and 118.95%, respectively. Based on the gall indexes, cluster analysis of hot pepper germplasms was performed. The 67 hot pepper germplasms were divided into 4 groups （resistant, moderately resistant, susceptible and high susceptible）. The resistance intensity of the hot pepper germplasms were ranked according to the sum of subordinate function values of various resistance indexes. The total function values of Rela 2 and L506M were the largest （2.00）, indicating that these two germplasms were immune to M. incognita. The total function values of L287-2, L522-1M, L504M, L515-2, 13SM100-1, L512M, L292-1, L319, L316, L317, 13SM87-1 and Rela 5 were larger than 1.95, indicating that these germplasms were highly resistant to M. incognita. [Conclusion] This study could provide certain resistant resources for resistance breeding of hot pepper to M. incognita.
基金supported by the National Key Technology Research and Development Program of China(2011BAZ01732)the Project of the Ministry of Agriculture of China(CARS-25)+2 种基金the Core Research Budget of the Non-profit Governmental Research Institute,China(ICS,CAAS1610032011011)the Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences(CAAS-ASTIP-IVFCAAS)
文摘Pepper（Capsicum spp.） is an important vegetable crop in the world. Now the pepper in China contributes one-third of the world＇s peppers production. Genetic diversity of the pepper germplasm of China is expected interesting to know. To explore the structure of genetic diversity in Chinese pepper germplasm resources and possible relationship with cultivar types or geographic origin, we sampled and compared 372 Gen Bank pepper accessions（local cultivars and landraces） from 31 provinces, autonomous regions and municipalities of China and 31 additional accessions from other countries. These accessions were genotyped using 28 simple sequence repeat（SSR） markers spanning the entire pepper genome. We then investigated the genetic structure of the sampled collection using model-based analysis in STRUCTURE v2.3.4 and examined genetic relationships by the unweighted pair-group method of mathematical averages（UPGMA） in MEGA. In addition to geographic origin, we evaluated eight plant and fruit traits. In total, 363 alleles were amplified using the 28 SSR primers. Gene diversity, polymorphism information content and heterozygosity of the 28 SSR loci were estimated as 0.09–0.92, 0.08–0.92 and 0.01–0.34, respectively. The UPGMA cluster analysis clearly distinguished Capsicum annuum L. from other cultivated pepper species. Population structure analysis of the 368 C. annuum accessions uncovered three genetic groups which also corresponded to distinct cultivar types with respect to the plant and fruit descriptors. The genetic structure was also related to the geographic origin of the landraces. Overall results indicate that genetic diversity of Chinese pepper landraces were structured by migration of genotypes followed by human selection for cultivar types in agreement with consumption modes and adaptation to the highly diversified agro-climatic conditions.
文摘The present study was carried out in the form of a survey with the aim of listing and describing the technical itineraries of production, storage and conservation used in six departments of high production of chilli pepper in Benin. The survey was conducted on the basis of a questionnaire in the six departments of Benin: Ouémé, Plateau, Atlantic, Littoral, Zou and Collines. The survey collected information on the socio-economic characteristics of producers, the varieties of chilli pepper grown, and the current technical itineraries used for the production, storage and conservation of chilli pepper. On the other hand, it also gave an idea of the constraints that prevent the production of chilli pepper of good sanitary quality. The results of this survey show that three varieties of chilli peppers are the most produced and consumed in Benin. These are the Gbotakin variety (<i>Capsicum</i> <i>chinense</i>), the Afundja variety (<i>Capsicum</i> <i>annuum</i>) and the Danhomètakin variety (<i>Capsicum</i> <i>frutescens</i>). The technical itineraries related to the cultivation of these chilli peppers varieties are the same in the six study departments. Unfortunately, their contamination sometimes starts at the nursery stage, passing through the field where other contaminants are added and develop until harvest. After harvest, contamination can continue in storage and finally reach the consumer if proper measures are not taken. The inappropriate or inadequate use of phytosanitary products in the chilli pepper industry is also a source of illness for consumers of this vegetable fruit. Training of producers on the best technical itineraries for the production and storage of chilli peppers is essential to revitalize and develop this sector by improving the sanitary quality of the chilli peppers produced in Benin.
