Hybridization and introgression have long obscured relationships within Adenophora and its relatives,complicating generic delimitation.Leveraging deep genome skimming(DGS)data,we generated a large dataset,including th...Hybridization and introgression have long obscured relationships within Adenophora and its relatives,complicating generic delimitation.Leveraging deep genome skimming(DGS)data,we generated a large dataset,including thousands of single-copy nuclear(SCN)genes and plastomes,to untangle this reticulate history.Specifically,9.89 terabytes(TB)of DGS data from 165 samples—representing 48 species and 13 subspecies of Adenophora(out of ca.72 species)plus 24 outgroup species—yielded 1506 SCN genes and 77 plastid coding sequences.Tree-like phylogenies inferred with both coalescent-and concatenation-based methods revealed pronounced gene tree heterogeneity.Subsequent analysis showed that incomplete lineage sorting contributed minimally to this discordance;instead,hybridization and introgression were the primary drivers of early diversification.Integrating phylogenomic,morphological,and geographic evidence,we propose a revised generic framework for this group.Adenophora is expanded to include Campanula delavayi and the Korean Peninsula endemic genus Hanabusaya.We also recommend reinstating Hyssaria as a distinct Central Asian genus and introducing two new genera,Boreoasia and Rosomala.展开更多
[Objectives] To study the original plant,traits and microscopic identification for Yi medicinal material Campanula colorata,and provide experimental data for its identification and medicinal use. [Methods] Plant ident...[Objectives] To study the original plant,traits and microscopic identification for Yi medicinal material Campanula colorata,and provide experimental data for its identification and medicinal use. [Methods] Plant identification,trait identification,powder identification and paraffin slice identification were adopted in the study. [Results]It was found that the original plant of Campanula colorata was characterized by stiff hairs,slightly thicker root than stem,serrated leaf margin,tubular bell-shaped corolla and blue-purple color; traits were featured by odorlessness,and bitter taste after slight sweetness; the microscopic identification characteristics were that the laticifer was sporadic,containing reddish brown or yellow brown materials,and the oil droplets and nonglandular hairs were common. [Conclusions] The original plant,trait and microscopic identification characteristics were obvious for Yi medicinal material Campanula colorata,which could provide a reference for developing quality standards and identifying the original plant of Campanula colorata,and provide the basis for further research and development.展开更多
The distant hybridization was used in lodging-resistance breeding of Platycodon grandiflorus. The parents were Platycodon grandiflorus (♀) and Campanula medium (♂). 187 seeds of F1 were harvested by using the inter-...The distant hybridization was used in lodging-resistance breeding of Platycodon grandiflorus. The parents were Platycodon grandiflorus (♀) and Campanula medium (♂). 187 seeds of F1 were harvested by using the inter-generic hybridization in 2009 and 2010, 2 repeat, and 88 normal plants were obtained. The characteristics of leaves, stems and branches were the same as female for F1 generation, F2 generation, backcross generation and S1 generation, and there were some different characteristics with female which were flower colors, branching habit and plant height. A lodging-resistance plant was selected in F1, which was about 75 cm high, deep pink flower color and developed lateral branches. The DNA groups of each generation were separated by 1% agarose gel electrophoresis and there were not significant differences. Among the each generation many flower colors and forms were obtained.展开更多
基金supported by the National Natural Science Foundation of China(grant numbers 32270216 and 32000163 to B.B.L.)the Youth Innovation Promotion Association CAS(2023086 to B.B.L.)the Biological Resources Programme,Chinese Academy of Sciences(CAS-TAX-24-013 to B.B.L.).
文摘Hybridization and introgression have long obscured relationships within Adenophora and its relatives,complicating generic delimitation.Leveraging deep genome skimming(DGS)data,we generated a large dataset,including thousands of single-copy nuclear(SCN)genes and plastomes,to untangle this reticulate history.Specifically,9.89 terabytes(TB)of DGS data from 165 samples—representing 48 species and 13 subspecies of Adenophora(out of ca.72 species)plus 24 outgroup species—yielded 1506 SCN genes and 77 plastid coding sequences.Tree-like phylogenies inferred with both coalescent-and concatenation-based methods revealed pronounced gene tree heterogeneity.Subsequent analysis showed that incomplete lineage sorting contributed minimally to this discordance;instead,hybridization and introgression were the primary drivers of early diversification.Integrating phylogenomic,morphological,and geographic evidence,we propose a revised generic framework for this group.Adenophora is expanded to include Campanula delavayi and the Korean Peninsula endemic genus Hanabusaya.We also recommend reinstating Hyssaria as a distinct Central Asian genus and introducing two new genera,Boreoasia and Rosomala.
基金Supported by National Science and Technology Support Program(2015BAC05-B02)Sichuan Science and Technology Support Program(2015SZ0034)+1 种基金Education and Teaching Research and Reform Project of Southwest University for Nationalities(2015)National Development Project for Central Universities-Southwest University for Nationalities(2016NGJPY11)
文摘[Objectives] To study the original plant,traits and microscopic identification for Yi medicinal material Campanula colorata,and provide experimental data for its identification and medicinal use. [Methods] Plant identification,trait identification,powder identification and paraffin slice identification were adopted in the study. [Results]It was found that the original plant of Campanula colorata was characterized by stiff hairs,slightly thicker root than stem,serrated leaf margin,tubular bell-shaped corolla and blue-purple color; traits were featured by odorlessness,and bitter taste after slight sweetness; the microscopic identification characteristics were that the laticifer was sporadic,containing reddish brown or yellow brown materials,and the oil droplets and nonglandular hairs were common. [Conclusions] The original plant,trait and microscopic identification characteristics were obvious for Yi medicinal material Campanula colorata,which could provide a reference for developing quality standards and identifying the original plant of Campanula colorata,and provide the basis for further research and development.
文摘The distant hybridization was used in lodging-resistance breeding of Platycodon grandiflorus. The parents were Platycodon grandiflorus (♀) and Campanula medium (♂). 187 seeds of F1 were harvested by using the inter-generic hybridization in 2009 and 2010, 2 repeat, and 88 normal plants were obtained. The characteristics of leaves, stems and branches were the same as female for F1 generation, F2 generation, backcross generation and S1 generation, and there were some different characteristics with female which were flower colors, branching habit and plant height. A lodging-resistance plant was selected in F1, which was about 75 cm high, deep pink flower color and developed lateral branches. The DNA groups of each generation were separated by 1% agarose gel electrophoresis and there were not significant differences. Among the each generation many flower colors and forms were obtained.
