The Chinese tree shrew has gained prominence as a model organism due to its phylogenetic proximity to primates,offering distinct advantages over traditional rodent models in biomedical research.However,the neuroanatom...The Chinese tree shrew has gained prominence as a model organism due to its phylogenetic proximity to primates,offering distinct advantages over traditional rodent models in biomedical research.However,the neuroanatomy of this species remains insufficiently defined,limiting its utility in neurophysiological and neuropathological studies.In this study,immunofluorescence microscopy was employed to comprehensively map the distribution of three calciumbinding proteins,parvalbumin,calbindin D-28k,and calretinin,across the tree shrew cerebrum.Serial brain sections in sagittal,coronal,and horizontal planes from 12 individuals generated a dataset of 3638 cellular-resolution images.This dataset,accessible via Science Data Bank(https://doi.org/10.57760/sciencedb.23471),provides detailed region-and laminar-selective distributions of calcium-binding proteins valuable for the cyto-and chemoarchitectural characterization of the tree shrew cerebrum.This resource will not only advance our understanding of brain organization and facilitate basic and translational neuroscience research in tree shrews but also enhance comparative and evolutionary analyses across species.展开更多
本实验应用原位杂交组织化学技术,利用同位素标记的寡核苷酸探针,对大鼠前脑含Calbindin-D28 K mRNA的神经元的分布状况进行了详细的观察。结果发现在不同脑区或核团中,标记神经元的数量和标记强度各不相同。某些部位含许多强阳性神经元...本实验应用原位杂交组织化学技术,利用同位素标记的寡核苷酸探针,对大鼠前脑含Calbindin-D28 K mRNA的神经元的分布状况进行了详细的观察。结果发现在不同脑区或核团中,标记神经元的数量和标记强度各不相同。某些部位含许多强阳性神经元,如:前嗅核、大脑皮质、尾壳核、缰核、下丘脑、齿状回及中脑和杏仁复合体中的部分核团;然而,在另外一些脑区中,标记细胞呈中等阳性,如:嗅球的球旁细胞、盖带、梨状区内核、海马的CA1区中的锥体细胞层以及丘脑和杏仁核复合体中的部分核团。少数脑区中的标记细胞呈弱阳性,且数量较少,如;嗅结节、隔区、斜角带核等。这些结果表明含Calbindin-D28K mRNA的神经元在大鼠前脑中具有区域特异性分布特点,从而提示Calbindin-D28K在神经系统中的某些部位可能具有重要的作用。展开更多
基金supported by the Science and Technology Innovation(STI)2030-Major Projects(2022ZD0205000 to L.L.)CAS“Light of West China”Program(xbzg-zdsys-202404 to L.L.)+1 种基金Yunnan Revitalization Talent Support Program Yunling Scholar Project(to L.L.)Yunnan Fundamental Research Projects(202305AH340006,202301AS070060 to L.L.,202401AT070206 to X.C.)。
文摘The Chinese tree shrew has gained prominence as a model organism due to its phylogenetic proximity to primates,offering distinct advantages over traditional rodent models in biomedical research.However,the neuroanatomy of this species remains insufficiently defined,limiting its utility in neurophysiological and neuropathological studies.In this study,immunofluorescence microscopy was employed to comprehensively map the distribution of three calciumbinding proteins,parvalbumin,calbindin D-28k,and calretinin,across the tree shrew cerebrum.Serial brain sections in sagittal,coronal,and horizontal planes from 12 individuals generated a dataset of 3638 cellular-resolution images.This dataset,accessible via Science Data Bank(https://doi.org/10.57760/sciencedb.23471),provides detailed region-and laminar-selective distributions of calcium-binding proteins valuable for the cyto-and chemoarchitectural characterization of the tree shrew cerebrum.This resource will not only advance our understanding of brain organization and facilitate basic and translational neuroscience research in tree shrews but also enhance comparative and evolutionary analyses across species.
文摘本实验应用原位杂交组织化学技术,利用同位素标记的寡核苷酸探针,对大鼠前脑含Calbindin-D28 K mRNA的神经元的分布状况进行了详细的观察。结果发现在不同脑区或核团中,标记神经元的数量和标记强度各不相同。某些部位含许多强阳性神经元,如:前嗅核、大脑皮质、尾壳核、缰核、下丘脑、齿状回及中脑和杏仁复合体中的部分核团;然而,在另外一些脑区中,标记细胞呈中等阳性,如:嗅球的球旁细胞、盖带、梨状区内核、海马的CA1区中的锥体细胞层以及丘脑和杏仁核复合体中的部分核团。少数脑区中的标记细胞呈弱阳性,且数量较少,如;嗅结节、隔区、斜角带核等。这些结果表明含Calbindin-D28K mRNA的神经元在大鼠前脑中具有区域特异性分布特点,从而提示Calbindin-D28K在神经系统中的某些部位可能具有重要的作用。
基金This study was supported by IAP grant P5 /20 of the Federal Belgian goverment and FWO grant G.0185.01 (to J.-P.T. and D. A.),and by a concerted research project granted by the Special Research Fund of the University of antwerp (to J.-P.T.)
