Hidden natural products are representative of defensive strategies produced in vivo in diseased plants,a process that is induced by the plant immune system.The first transcriptome library of uninfected and pathogen in...Hidden natural products are representative of defensive strategies produced in vivo in diseased plants,a process that is induced by the plant immune system.The first transcriptome library of uninfected and pathogen infected Hibiscus tiliaceus stems was constructed by transcriptome sequencing technology,genes related to cadinene-type sesquiterpenoid biosynthesis were screened and combined with ultra-performance liquid chromatography-quadrupole-time of flight mass spectrometry(UPLC-QTOF-MS)analysis data,which indicated pathological tissue had potential to produce novel carbon skeletons of cadinane sesquiterpenoid dimers.Successfully,two cadinane-derived sesquiterpenoid dimers with unprecedented carbon skeletons,hibisceusanols A(1)and B(2)were isolated for the first time from the stems of H.tiliaceus induced by plant-microbial interactions.Their structures and absolute configurations were unambiguously established by spectroscopy,advanced chemistry development(ACD)and electronic circular dichroism(ECD)methods.Compounds 1 and 2 exhibited significant antitumor activity in vitro with half maximal inhibitory concentration(IC_(5)0)values of 2.3–7.2μmol/L.The anticancer effect was generated via the induction of Hep G2 cell apoptosis by inhibiting the phosphatidylinositol 3-kinase(PI3K)pathway.展开更多
基金financially supported by the CAMS Innovation Fund for Medical Sciences(CIFMS)(Nos.2021-I2M-1-032,2021I2M-1-071)the Key Research Project of Hainan Province(No.ZDYF2020111)Project supported by the Research Fund for Development Program of Beijing City University(No.KYF202003)。
文摘Hidden natural products are representative of defensive strategies produced in vivo in diseased plants,a process that is induced by the plant immune system.The first transcriptome library of uninfected and pathogen infected Hibiscus tiliaceus stems was constructed by transcriptome sequencing technology,genes related to cadinene-type sesquiterpenoid biosynthesis were screened and combined with ultra-performance liquid chromatography-quadrupole-time of flight mass spectrometry(UPLC-QTOF-MS)analysis data,which indicated pathological tissue had potential to produce novel carbon skeletons of cadinane sesquiterpenoid dimers.Successfully,two cadinane-derived sesquiterpenoid dimers with unprecedented carbon skeletons,hibisceusanols A(1)and B(2)were isolated for the first time from the stems of H.tiliaceus induced by plant-microbial interactions.Their structures and absolute configurations were unambiguously established by spectroscopy,advanced chemistry development(ACD)and electronic circular dichroism(ECD)methods.Compounds 1 and 2 exhibited significant antitumor activity in vitro with half maximal inhibitory concentration(IC_(5)0)values of 2.3–7.2μmol/L.The anticancer effect was generated via the induction of Hep G2 cell apoptosis by inhibiting the phosphatidylinositol 3-kinase(PI3K)pathway.
