Anthracnose,caused by Colletotrichum camelliae,poses a significant threat to the yield and quality of Camellia oleifera.Mitophagy,a selective form of autophagy,is crucial in maintaining mitochondrial quality and intra...Anthracnose,caused by Colletotrichum camelliae,poses a significant threat to the yield and quality of Camellia oleifera.Mitophagy,a selective form of autophagy,is crucial in maintaining mitochondrial quality and intracellular homeostasis.To date,an optimized experimental system for studying mitophagy in C.camelliae has yet to be established,and the role of mitophagy in the pathogenesis of C.camelliae remains unclear.Here,we characterized the function of CaEch1,a homolog of Magnaporthe oryza MoEch1,in the anthracnose fungus C.camelliae.Our findings indicate that mitochondria-localized CaEch1,plays a vital role for mitophagy in C.camelliae.Also,the CaEch11-29-GFP could serve as a reliable marker for monitoring mitophagy in C.camelliae.Additionally,the knockout of CaECH1 resulted in defects in fungal growth,conidiation,and appressorium formation.Pathogenicity assays further revealed that knockout of CaECH1 significantly reduced the virulence of C.camelliae.In summary,our research underscores the importance of CaEch1 in growth,conidiation,appressorium formation,and virulence in C.camelliae.This suggests its potential as a marker for mitophagy and provides valuable insights for developing new fungicides targeting anthracnose.展开更多
基金supported by the Zhejiang Provincial Natural Science Foundation of China(Grant No.LQ24C160010)the‘Pioneer’and‘Leading Goose’R&D Program of Zhejiang(Grant No.2023C02034)the Scientific Research Foundation of Zhejiang Agriculture and Forestry University(2022LFR101).
文摘Anthracnose,caused by Colletotrichum camelliae,poses a significant threat to the yield and quality of Camellia oleifera.Mitophagy,a selective form of autophagy,is crucial in maintaining mitochondrial quality and intracellular homeostasis.To date,an optimized experimental system for studying mitophagy in C.camelliae has yet to be established,and the role of mitophagy in the pathogenesis of C.camelliae remains unclear.Here,we characterized the function of CaEch1,a homolog of Magnaporthe oryza MoEch1,in the anthracnose fungus C.camelliae.Our findings indicate that mitochondria-localized CaEch1,plays a vital role for mitophagy in C.camelliae.Also,the CaEch11-29-GFP could serve as a reliable marker for monitoring mitophagy in C.camelliae.Additionally,the knockout of CaECH1 resulted in defects in fungal growth,conidiation,and appressorium formation.Pathogenicity assays further revealed that knockout of CaECH1 significantly reduced the virulence of C.camelliae.In summary,our research underscores the importance of CaEch1 in growth,conidiation,appressorium formation,and virulence in C.camelliae.This suggests its potential as a marker for mitophagy and provides valuable insights for developing new fungicides targeting anthracnose.
