The regulatory mechanisms of cytoplasmic Ca2+after myocardial infarction-induced Ca2+overload involve secretory pathway Ca2+-ATPase 1 and the Golgi apparatus and are well understood.However,the effect of Golgi apparat...The regulatory mechanisms of cytoplasmic Ca2+after myocardial infarction-induced Ca2+overload involve secretory pathway Ca2+-ATPase 1 and the Golgi apparatus and are well understood.However,the effect of Golgi apparatus on Ca2+overload after cerebral ischemia and reperfusion remains unclear.Four-vessel occlusion rats were used as animal models of cerebral ischemia.The expression of secretory pathway Ca2+-ATPase 1 in the cortex and hippocampus was detected by immunoblotting,and Ca2+concentrations in the cytoplasm and Golgi vesicles were determined.Results showed an overload of cytoplasmic Ca2+during ischemia and reperfusion that reached a peak after reperfusion.Levels of Golgi Ca2+showed an opposite effect.The expression of Golgi-specific secretory pathway Ca2+-ATPase 1 in the cortex and hippocampus decreased before ischemia and reperfusion,and increased after reperfusion for 6 hours.This variation was similar to the alteration of calcium in separated Golgi vesicles.These results indicate that the Golgi apparatus participates in the formation and alleviation of calcium overload,and that secretory pathway Ca2+-ATPase 1 tightly responds to ischemia and reperfusion in nerve cells.Thus,we concluded that secretory pathway Ca2+-ATPase 1 plays an essential role in cytosolic calcium regulation and its expression can be used as a marker of Golgi stress,responding to cerebral ischemia and reperfusion.The secretory pathway Ca2+-ATPase 1 can be an important neuroprotective target of ischemic stroke.展开更多
基金supported by the National Natural Science Foundation of China,No.81171239
文摘The regulatory mechanisms of cytoplasmic Ca2+after myocardial infarction-induced Ca2+overload involve secretory pathway Ca2+-ATPase 1 and the Golgi apparatus and are well understood.However,the effect of Golgi apparatus on Ca2+overload after cerebral ischemia and reperfusion remains unclear.Four-vessel occlusion rats were used as animal models of cerebral ischemia.The expression of secretory pathway Ca2+-ATPase 1 in the cortex and hippocampus was detected by immunoblotting,and Ca2+concentrations in the cytoplasm and Golgi vesicles were determined.Results showed an overload of cytoplasmic Ca2+during ischemia and reperfusion that reached a peak after reperfusion.Levels of Golgi Ca2+showed an opposite effect.The expression of Golgi-specific secretory pathway Ca2+-ATPase 1 in the cortex and hippocampus decreased before ischemia and reperfusion,and increased after reperfusion for 6 hours.This variation was similar to the alteration of calcium in separated Golgi vesicles.These results indicate that the Golgi apparatus participates in the formation and alleviation of calcium overload,and that secretory pathway Ca2+-ATPase 1 tightly responds to ischemia and reperfusion in nerve cells.Thus,we concluded that secretory pathway Ca2+-ATPase 1 plays an essential role in cytosolic calcium regulation and its expression can be used as a marker of Golgi stress,responding to cerebral ischemia and reperfusion.The secretory pathway Ca2+-ATPase 1 can be an important neuroprotective target of ischemic stroke.
