Aortic stenosis(AS)is the most common valvular heart disease,with a prevalence of over 4%among octogenarians.[1]The prevalence of autopsy-confirmed wild-type transthyretin cardiac amyloidosis(ATTRwt-CA)increases with ...Aortic stenosis(AS)is the most common valvular heart disease,with a prevalence of over 4%among octogenarians.[1]The prevalence of autopsy-confirmed wild-type transthyretin cardiac amyloidosis(ATTRwt-CA)increases with age,accounting for 25%of patients aged 85 years and older in Europe and 12%of patients older than 80 years in Japan.[2,3]Recent studies have reported that ATTRwt-CA coexists in 11%-16%of older patients with AS undergoing transcatheter aortic valve replacement(TAVR).[1,4,5]In a metaanalysis by Ho et al.,[6]the prevalence rates of cardiac amyloidosis,predominantly ATTRwt-CA,in patients with AS and those referred for TAVR or surgical aortic valve replacement(SAVR)were 14.4%and 15.2%,respectively.Conversely,the prevalence of AS in patients with cardiac amyloidosis is 8.7%.Owing to the high surgical risk in patients with both AS and ATTRwt-CA,TAVR may be preferred over SAVR.展开更多
Clustered regularly interspaced short palindromic repeats(CRISPR)systems have achieved significant advancements in precise molecular diagnosis.However,their applications in whole blood detection remain challenging due...Clustered regularly interspaced short palindromic repeats(CRISPR)systems have achieved significant advancements in precise molecular diagnosis.However,their applications in whole blood detection remain challenging due to signal interference from blood autofluorescence.Here,we proposed a universal and accessible bioluminescent CRISPR/Cas(bioLUCas)platform for direct detection of disease biomarkers in whole blood.By employing a specially designed cpHNLucMB reporter,the bioLUCas system converts CRISPR/Cas12a trans-cleavage activity into a ratiometric bioluminescent signal,producing a distinct emission color change.Compared to conventional CRISPR/Cas12a-based sensors,this platform eliminates the need for external light excitation,effectively bypassing blood autofluorescence and offering high sensitivity.Additionally,the visual signal of bioLUCas system allows user-friendly readout methods,such as smartphone.The platform successfully facilitated point-of-care test(POCT)for myeloperoxidase(MPO)in clinical acute myelogenous leukemia(AML)blood samples and hepatitis C virus(HCV)RNA in synthetic blood samples.This work may advance CRISPR/Cas technology for accessible whole-blood disease diagnostics.展开更多
We report the results of the experiment on synthesizing ^(287,288)Mc isotopes (Z=115) using the fusionevaporation reaction ^(243)Am(^(48)Ca,4n,3n)^(287,288)Mc at the Spectrometer for Heavy Atoms and Nuclear Structure-...We report the results of the experiment on synthesizing ^(287,288)Mc isotopes (Z=115) using the fusionevaporation reaction ^(243)Am(^(48)Ca,4n,3n)^(287,288)Mc at the Spectrometer for Heavy Atoms and Nuclear Structure-2(SHANS2),a gas-filled recoil separator located at the China Accelerator Facility for Superheavy Elements(CAFE2).In total,20 decay chains are attributed to ^(288)Mc and 1 decay chain is assigned to ^(287)Mc.The measured oa-decay properties of ^(287,288)Mc as well as its descendants are consistent with the known data.No additional decay chains originating from the 2n or 5n reaction channels were detected.The excitation function of the ^(243)Am(^(48)Ca,3n)^(288)Mc reaction was measured at the cross-section level of picobarn,which indicates the promising capability for the study of heavy and superheavy nuclei at the facility.展开更多
文摘Aortic stenosis(AS)is the most common valvular heart disease,with a prevalence of over 4%among octogenarians.[1]The prevalence of autopsy-confirmed wild-type transthyretin cardiac amyloidosis(ATTRwt-CA)increases with age,accounting for 25%of patients aged 85 years and older in Europe and 12%of patients older than 80 years in Japan.[2,3]Recent studies have reported that ATTRwt-CA coexists in 11%-16%of older patients with AS undergoing transcatheter aortic valve replacement(TAVR).[1,4,5]In a metaanalysis by Ho et al.,[6]the prevalence rates of cardiac amyloidosis,predominantly ATTRwt-CA,in patients with AS and those referred for TAVR or surgical aortic valve replacement(SAVR)were 14.4%and 15.2%,respectively.Conversely,the prevalence of AS in patients with cardiac amyloidosis is 8.7%.Owing to the high surgical risk in patients with both AS and ATTRwt-CA,TAVR may be preferred over SAVR.
基金supported by the National Natural Science Foundation of China(22474032,22104032)to Mengyi Xiong and(22234003,21890744)to Xiao-Bing Zhangthe Natural Science Foundation of Shanxi Province(202303021221139)to Xuhua Zhao+1 种基金the China Postdoctoral Science Foundation(2024M761887)to Xuhua Zhaothe Innovation and Entrepreneurship Training Program for College Students(20161469)。
文摘Clustered regularly interspaced short palindromic repeats(CRISPR)systems have achieved significant advancements in precise molecular diagnosis.However,their applications in whole blood detection remain challenging due to signal interference from blood autofluorescence.Here,we proposed a universal and accessible bioluminescent CRISPR/Cas(bioLUCas)platform for direct detection of disease biomarkers in whole blood.By employing a specially designed cpHNLucMB reporter,the bioLUCas system converts CRISPR/Cas12a trans-cleavage activity into a ratiometric bioluminescent signal,producing a distinct emission color change.Compared to conventional CRISPR/Cas12a-based sensors,this platform eliminates the need for external light excitation,effectively bypassing blood autofluorescence and offering high sensitivity.Additionally,the visual signal of bioLUCas system allows user-friendly readout methods,such as smartphone.The platform successfully facilitated point-of-care test(POCT)for myeloperoxidase(MPO)in clinical acute myelogenous leukemia(AML)blood samples and hepatitis C virus(HCV)RNA in synthetic blood samples.This work may advance CRISPR/Cas technology for accessible whole-blood disease diagnostics.
基金supported in part by the National Key R&D Program of China (Contract Nos.2023YFA1606500,2024YFE0109800,and 2024YFE0110400)Strategic Priority Research Program of Chinese Academy of Sciences(Grant No.XDB34010000)+5 种基金the Gansu Key Project of Science and Technology (Grant No.23ZDGA014)the Guangdong Major Project of Basic and Applied Basic Research (Grant No.2021B0301030006)the National Natural Science Foundation of China (Grant Nos.12105328,W2412040,12475126,12422507,12035011,12375118,12435008,and W2412043)the Chinese Academy of Sciences Project for Young Scientists in Basic Research(Grant No.YSBR-002)the Youth Innovation Promotion Association of the Chinese Academy of Sciences (Grant Nos.2020409 and 2023439)the Russian Science Foundation (Grant No.25-42-00003)。
文摘We report the results of the experiment on synthesizing ^(287,288)Mc isotopes (Z=115) using the fusionevaporation reaction ^(243)Am(^(48)Ca,4n,3n)^(287,288)Mc at the Spectrometer for Heavy Atoms and Nuclear Structure-2(SHANS2),a gas-filled recoil separator located at the China Accelerator Facility for Superheavy Elements(CAFE2).In total,20 decay chains are attributed to ^(288)Mc and 1 decay chain is assigned to ^(287)Mc.The measured oa-decay properties of ^(287,288)Mc as well as its descendants are consistent with the known data.No additional decay chains originating from the 2n or 5n reaction channels were detected.The excitation function of the ^(243)Am(^(48)Ca,3n)^(288)Mc reaction was measured at the cross-section level of picobarn,which indicates the promising capability for the study of heavy and superheavy nuclei at the facility.
