Ovarian cancer remains a leading cause of gynecological cancer mortality1,and patients with advanced stage ovarian cancer frequently develop malignant ascites that foster immunosuppressive microenvironments and therap...Ovarian cancer remains a leading cause of gynecological cancer mortality1,and patients with advanced stage ovarian cancer frequently develop malignant ascites that foster immunosuppressive microenvironments and therapeutic resistance2,3.Although ascites have traditionally been considered detrimental,we report a paradoxical role in which they enhance the cytotoxicity ofγδT cells—a unique T cell subset that can be allogenically transferred for cancer treatment4,5—toward ovarian cancer.展开更多
Phytochemical study of the n-BuOH extract of Ilex asprella resulted in the discovery of ten new pentacyclic triterpenoid glycosides,comprising nine ursane-type glycosides(1-9)and one oleanane-type glycoside(10),along ...Phytochemical study of the n-BuOH extract of Ilex asprella resulted in the discovery of ten new pentacyclic triterpenoid glycosides,comprising nine ursane-type glycosides(1-9)and one oleanane-type glycoside(10),along with seven known compounds(11-17).Compound 1 is the first reported 19,22-epoxy ursane triterpenoid glycoside,whereas 4 and 5 are rare examples of ursane triterpenoid glycosides containing a 28,19-lactone group.The structural characterization of these compounds was achieved using spectroscopic and chemical techniques,as well as single-crystal X-ray analysis.Compounds 7,12,15,and 17 exhibited moderate cytotoxic activities against H1975 and HCC827 cancer cells.展开更多
(±)-Penicithrones A–D(1a/1b–4a/4b),four novel pairs of anthrone–cyclopentenone heterodimers characterized by a distinctive bridged 6/6/6−5 tetracyclic core skeleton,together with three previously identified co...(±)-Penicithrones A–D(1a/1b–4a/4b),four novel pairs of anthrone–cyclopentenone heterodimers characterized by a distinctive bridged 6/6/6−5 tetracyclic core skeleton,together with three previously identified compounds(5–7),were isolated from the crude extract of the mangrove-derived fungus Penicillium sp.,guided by heteronuclear single quantum correlation(HSQC)-based small molecule accurate recognition technology(SMART 2.0)and liquid chromatography-tandem mass spectrometry(LC-MS/MS)-based molecular networking.The structural elucidation of new compounds was accomplished through comprehensive spectroscopic analysis,and their absolute configurations were determined using DP4+^(13)C nuclear magnetic resonance(NMR)calculations and electronic circular dichroism(ECD)calculations.Compounds 1a/1b–4a/4b demonstrated moderate cytotoxicity against three human cancer cell lines HeLa,HCT116 and MCF-7 with half maximal inhibitory concentration(IC50)values ranging from 15.95±1.64 to 28.56±2.59μmol·L–1.展开更多
Antipalu is a phytomedicinal medicinal beverage that is popular in the District of Abidjan, particularly for the treatment of malaria. However, Antipalu could present potential health effects on patients, and few toxi...Antipalu is a phytomedicinal medicinal beverage that is popular in the District of Abidjan, particularly for the treatment of malaria. However, Antipalu could present potential health effects on patients, and few toxicological studies have been conducted before its use. In order to determine the cytotoxicity of Antipalu, two complementary tests, LDH activity and the MTT cell proliferation assay, were used using Vero cells. Vero cells were exposed to increasing concentrations of Antipalu and incubated for 24, 48 and 72 hours. In addition, forty (40) rats distributed randomly into 4 groups, including 10 animals per group (5 males and 5 females) were used for the potential hepatoxic effects. Animals in group 1 received distilled water and were used as a control group. On the other hand, Lot I, II and III received by gavage a volume of the Antipalu extract corresponding to 1 ml/100 g of body weight at 200 mg/kg, 400 mg/kg, 800 mg/kg, respectively. The extract was administered daily at the same time for 28 days and serum was collected once a week to evaluate hepatic biochemical markers. After 28 days of study, all rats were euthanized by an overdose of ether and the liver of the rats was removed for gross morphological and histopathological analysis. The results of the cell supernatant assay showed an increasing extracellular LDH enzyme activity with lethal concentrations at 10% and 50% (LC10 = 111 µg/mL and LC50 = 555 µg/mL, respectively). In addition, the MTT assay showed a decrease in mitochondrial activity and thus cell proliferation after 24, 48 and 72 H of incubation. Our study showed that Antipalu caused alterations in the plasma membranes of the cells, resulting in the release of lactase dehydrogenase (LDH) into the external environment and a decrease in the mitochondrial activity of the Vero cells. The biochemical parameters ALT, ASAT, ALPs, and GGT showed no significant change (P > 0.05) in the group of treated rats compared to the controls. However, these variations were moderate and transient, with values remaining almost within their standard limits. Microscopic observations of liver tissue sections from rats treated with the Antipalu showed no lesions, edema and necrosis. These results suggest that the Antipalu did not interfere with the functioning or alter the integrity of the liver.展开更多
Five novel emestrin-type epipolythiodioxopiperazines(ETPs),prenylemestrins C-G(1-5),along with two known ETPs,prenylemestrin A(6)and prenylemestrin B(7),were obtained from Aspergillus nidulans.Their structures were ch...Five novel emestrin-type epipolythiodioxopiperazines(ETPs),prenylemestrins C-G(1-5),along with two known ETPs,prenylemestrin A(6)and prenylemestrin B(7),were obtained from Aspergillus nidulans.Their structures were characterized by spectroscopic data,X-ray crystallographic data,ECD comparisons and calculations.Prenylemestrins C-G(1-5)represent a rare class of ETPs,characterized by a 2,5-dithia-7,9-diazabicyclo[4.2.2]decane-8,10-dione core involving a hemiterpene moiety.Notably,compound 6 exhibited moderate cytotoxicity,inducing G2/M cell cycle arrest and apoptosis of L1210 cells by regulating the PI3K/AKT signaling pathway and mitochondrial apoptotic mechanisms.展开更多
The interest in using the Datura stramonium plant is due to its natural products,which are used in many pharmaceutical industries.The objective of the current study was to assess the therapeutic and cytotoxic effects ...The interest in using the Datura stramonium plant is due to its natural products,which are used in many pharmaceutical industries.The objective of the current study was to assess the therapeutic and cytotoxic effects of the D.stramonium plant on two types of human cancer cell models(MCF7 and HT29)in vitro.A soxhlet apparatus was used to obtain methanolic extract from dried plant leaves.The recovered crude,after the solvent had evaporated,was then dispersed at varied concentrations of extract 100,50,20,and 0.0µg/mL and tested to see how the cells responded.Also,the cancer-testis antigen(CTA)gene transcription in the two cell types exposed to the plant extract was examined using a semi-quantitative real-time polymerase chain reaction.Gas chromatography–mass spectrometry(GC-MS)results produced the significant main metabolites Nonanoic acid,Tropine N-Oxide,3,6-Ditigloyloxy-7-hydroxytropane,Hexadecanoic acid,2-Pentadecanone,6,10,14-trimethyl-,Carvenone,methyl ester,Phytol,Aposcopolamine,Hyoscyamine,4,8,12,16-Tetramethylheptadecan-4-olide,Scopolamine,Alpha.-Tocospiro A,1,2-Cycloheptanedione,3,3,7,7-tetramethyl-,dihydrazone,Campesterol,Stigmasterol,Gamma-Sitosterol and dl-.alpha.-Tocopherol.The results showed that the two types of cell lines impacted by D.stramonium extract,through untreated type 1 cells(MCF7)gave a highly significant transcription according to all applicable genes.All implemented analyses cleared the strong genetic impacts of Datura extract on cancer cells’genomes.TGIF2LY and C2orf63 transcript accumulation were also significantly elevated when exposed to plant extract at a level of 50µg/mL in cell line type 2(HT29),but TGIF2LY and P53 had the lowest relative expression at a level of 100µg/mL when treated the same cell line type.展开更多
Maternal consumption of a high-fat diet has been linked to increased risks of obesity and impaired glucose metabolism in offspring.However,the precise epigenetic mechanisms governing these intergenerational effects,pa...Maternal consumption of a high-fat diet has been linked to increased risks of obesity and impaired glucose metabolism in offspring.However,the precise epigenetic mechanisms governing these intergenerational effects,particularly during the early stages of offspring development,remain poorly understood.In this study,female C57BL/6J mice were randomly assigned to either a high-fat diet or normal chow diet throughout gestation and lactation.Methylated DNA immunoprecipitation(MeDIP)coupled with microarray analysis was employed to identify differentially methylated genes in the livers of offspring at weaning age.We found that maternal high-fat diet feeding predisposes offspring to obesity and impaired glucose metabolism as early as the weaning period.DNA methylation profile analysis unveiled a significant enrichment of differentially methylated genes within the natural killer(NK)cell-mediated cytotoxicity pathway.MeDIP-PCR validated reduced methylation levels of specific genes within this pathway,including tumour necrosis factorα(TNF-α),phosphoinositide 3-kinase(PI3K),and SHC adaptor protein 1(SHC1).Consistently,the expressions of TNF-α,PI3K,and SHC1 were significantly upregulated,accompanied by elevated serum TNF-αand interleukin-6(IL-6)levels in offspring from dams fed with high-fat diet.Moreover,we assessed the expressions of genes associated with NK cell activities,uncovering a notable rise in hepatic granzyme B levels and a trend towards increased CD107a expression in offspring from dams fed a high-fat diet.In addition,methylation levels of TNF-α,PI3K,and SHC1 promoters were inversely correlated with glucose response during glucose tolerance testing.In conclusion,our findings underscore the critical role of the NK cell-mediated cytotoxicity signaling pathway in mediating DNA methylation patterns,thereby contributing to the programming effects of maternal high-fat diet consumption on offspring glucose metabolism as early as the weaning period.展开更多
K−Na co-doped δ-MnO_(2)(KNMOH)nanoflowers were synthesized,and their cytotoxic effects against HeLa cervical cancer cells were evaluated.The KNMOH exhibited significant dose-and time-dependent cytotoxicity at concent...K−Na co-doped δ-MnO_(2)(KNMOH)nanoflowers were synthesized,and their cytotoxic effects against HeLa cervical cancer cells were evaluated.The KNMOH exhibited significant dose-and time-dependent cytotoxicity at concentrations of 50 and 100μg/mL.After 24 h of incubation treatment,cell viability decreased to(36.8±6.5)% and(33.4±6.4)%at 50 and 100μg/mL,respectively.With extended exposure to 48 h,cell viability was(45.2±2.3)%and(32.3±2.8)%at the same concentrations.Phase-contrast microscopy revealed characteristic morphological changes including cell shrinkage and membrane blebbing formation,indicative of cell death.These findings demonstrate the potential of KNMOH nanoflowers as a cytotoxic agent for cervical cancer applications and provide a foundation for further mechanistic studies.展开更多
Plasma electrolytic oxidation is a well-known technique for surface modification of biomedical magnesium alloys,with good corrosion protection and the ability to produce biocompatible and bioactive coatings.In this st...Plasma electrolytic oxidation is a well-known technique for surface modification of biomedical magnesium alloys,with good corrosion protection and the ability to produce biocompatible and bioactive coatings.In this study,calcium-phosphate coatings were produced on WE43 magnesium alloy for use,as orthopedic implants.Coating formation was prepared using different oxidation parameters with various duty ratios(DR)of 15,25 and 50%and current ratios(R)-2 or 1.6.Application of R with excess cathodic current(R>1)in processes with DR≥25%allowed attaining the soft-sparking regime(SSR)that resulted in thicker oxide coatings with higher degree of crystallinity compared to the films obtained without SSR.The results of the corrosion tests contributed to a noticeable improvement in the corrosion resistance of the magnesium alloy.Optimization of the oxidation parameters allowed the selection of the variants with the most favorable degradation behavior over the tested immersion period,indicating a successful modification of the magnesium alloy surface to obtain an implant biomaterial capable of providing controlled degradation.Furthermore,biological evaluation of the produced coatings showed that the proposed surface modifications significantly reduced the cytotoxic effects observed in direct contact with the material while still maintaining the cell proliferation-promoting effects of the material eluents.展开更多
Chemical investigations of the termite-associated Streptomyces tanashiensis BYF-112 resulted in the discovery of four novel alkaloid derivatives: vegfrecines A and B(1 and 2), exfoliazone A(3), and venezueline H(7), i...Chemical investigations of the termite-associated Streptomyces tanashiensis BYF-112 resulted in the discovery of four novel alkaloid derivatives: vegfrecines A and B(1 and 2), exfoliazone A(3), and venezueline H(7), in addition to nine known metabolites(4-6, 8-13). The structures of these compounds were elucidated through comprehensive spectroscopic analysis and comparison with existing literature data. Antibacterial assays revealed that viridomycin A(11) exhibited potent antibacterial activity against Staphylococcus aureus, with a zone of inhibition(ZOI) of 12.67 mm, in comparison to a ZOI of 17.67 mm for the positive control gentamicin sulfate. Viridomycin A(11) showed moderate activity against Micrococcus tetragenus and Pseudomonas syringae pv. actinidae, with ZOI values of 15.50 and 14.33 mm, respectively, which were inferior to those of gentamicin sulfate(34.67 and 24.00mm). Viridomycin F(12) also exhibited moderate antibacterial effects against S. aureus, M. tetragenus, and P. syringae pv. actinidae,with ZOI values of 8.33, 16.50, and 10.83 mm, respectively. Cytotoxicity assays demonstrated that viridobruunine A(5), exfoliazone(6), viridomycin A(11), and X-14881E(13) exhibited significant cytotoxicity against human malignant melanoma(A375), ovarian cancer(SKOV-3), and gastric cancer(MGC-803) cell lines, with IC_(50) values ranging from 4.61 to 19.28 μmol·L^(-1). Furthermore, bioinformatic analysis of the complete genome of S. tanashiensis suggested a putative biosynthetic gene cluster(BGC) responsible for the production of compounds 1-12. These findings indicate that the secondary metabolites of insect-associated S. tanashiensis BYF-112hold promise as potential sources of novel antibacterial and anticancer agents.展开更多
A phytochemical investigation of the whole plant of Parasenecio rubescens(S.Moore)Y.L.Chen yielded 14 previ-ously undescribed,highly oxidized bisabolane-type sesquiterpenoids,named pararunines L-Y,along with one known...A phytochemical investigation of the whole plant of Parasenecio rubescens(S.Moore)Y.L.Chen yielded 14 previ-ously undescribed,highly oxidized bisabolane-type sesquiterpenoids,named pararunines L-Y,along with one known oplopane-type sesquiterpenoid.The structural elucidation of these compounds was accomplished through comprehensive spectroscopic analysis,in-cluding nuclear magnetic resonance(NMR)and high-resolution electrospray ionization mass spectrometry(HR-ESI-MS)techniques.Motivated by traditional uses and previous studies on this genus,all isolated compounds were subjected to in vitro cytotoxicity assays against four human cancer cell lines(MCF-7,Hela,HCT116,and HT-29).Considering that the reported chemical constituents of nu-merous other species within this genus primarily consist of eremophilane-type sesquiterpenoids,our findings not only expand the struc-tural diversity of bisabolane-type sesquiterpenoids but also contribute valuable scientific evidence to the chemotaxonomy of this genus.展开更多
Two distinctive rearranged 19-nor-7,8-seco-labdane diterpenoids(1 and 2)with a novel tetracyclo[5.2.1.0^(2,5.)0^(4,10)]decane skeleton,a derivative of the open tetrahydrofuran ring(7),three dimeric compounds(8-10),and...Two distinctive rearranged 19-nor-7,8-seco-labdane diterpenoids(1 and 2)with a novel tetracyclo[5.2.1.0^(2,5.)0^(4,10)]decane skeleton,a derivative of the open tetrahydrofuran ring(7),three dimeric compounds(8-10),and four revised homologs(3-6)were obtained from Chinese liverwort Pallavicinia ambigua.Their structures were identified via combined analysis of their spectroscopic data,single-crystal X-ray diffraction patterns,and ECD calculations.The light-driven conversion of compound 5 to compounds 1-4 demonstrated that photochemically induced postmodification involved in biosynthesis is an important way to diversify natural structures.A preliminary cytotoxicity assay revealed that compound 5 showed significant inhibition in the human prostate cancer(PC-3)cell line via an apoptotic pathway.展开更多
Eight novel arylnaphthalide lactone lignans, designated as diphylignan A-H(1-8), and a new dibenzyltyrolactone lignan, designated as diphylignan I(9), were isolated from the roots and rhizomes of Diphylleia sinensis, ...Eight novel arylnaphthalide lactone lignans, designated as diphylignan A-H(1-8), and a new dibenzyltyrolactone lignan, designated as diphylignan I(9), were isolated from the roots and rhizomes of Diphylleia sinensis, along with two additional novel natural products(11 and 14) and four known metabolites(10, 12, 13, 15). The structural and stereochemical characterization of these compounds was accomplished using NMR spectroscopy and electronic circular dichroism(ECD) analysis. The cytotoxic activities of all isolated compounds were assessed against A-549 and SMMC-7721 cell lines. Notably, compound 2 demonstrated the most significant cytotoxicity, with IC_(50) values of 10.27 and 11.58 μmol·L^(-1) against A-549 and SMMC-7721 cell lines, respectively, exhibiting greater potency than the positive control, cisplatin.展开更多
Citrinsorbicillin A(1),a novel homotrimeric sorbicillinoid,along with two new monomers citrinsor-bicillins B(2)and C(3),were isolated from the Coptis chinensis endophyte Trichoderma citrinoviride HT-9 by liquid chroma...Citrinsorbicillin A(1),a novel homotrimeric sorbicillinoid,along with two new monomers citrinsor-bicillins B(2)and C(3),were isolated from the Coptis chinensis endophyte Trichoderma citrinoviride HT-9 by liquid chromatograph mass spectrometer(LC-MS)-guided strategy.1 was the first trimeric-example from terrestrial fungi,which possessed a unique carbon skeleton with two bicyclo[2.2.2]octanedione ring connected through an enolated carbon forming by[4+2]cycloaddition.Their structures were elucidated by spectroscopic analysis and X-ray diffraction.1 exhibited moderate cytotoxicity against human colon cancer HT29 cells,and it induced significant cell cycle arrest by reducing the protein expression of cyclin D1.展开更多
Four novel macrocyclic trichothecenes,termed mytoxins D-G(1-4),along with four known analogs(5-8),were isolated from the ethyl acetate extract of fermented rice inoculated with the fungus Myrothecium verrucaria PA57.E...Four novel macrocyclic trichothecenes,termed mytoxins D-G(1-4),along with four known analogs(5-8),were isolated from the ethyl acetate extract of fermented rice inoculated with the fungus Myrothecium verrucaria PA57.Each compound features a tricyclic 12,13-epoxytrichothec-9-ene(EPT)core.Notably,mytoxin G(4)represents the first instance of a macrocyclic trichothecene incorporating a glucosyl unit within the trichothecene structure.The structures of the newly identified compounds were elucidated through comprehensive spectroscopic analysis combined with quantum chemical calculations.All isolated compounds demonstrated cytotoxic activity against the CAL27 and HCT116 cell lines,which are models for human oral squamous cell carcinoma and colorectal cancer,respectively.Specifically,mytoxin D(1)and mytoxin F(3)exhibited pronounced cytotoxic effects against both cancer cell lines,with IC_(50)values ranging from 3 to 6 nmol·L^(-1).Moreover,compounds 1 and 3 were found to induce apoptosis in HCT116 cells by activating caspase-3.展开更多
Objective To modify the double bonds and hydroxyl groups of marine natural product brefeldin A(1),derivatives 2~10 were prepared and their inhibitory activities against lung adenocarcinoma epithelial A549 were evaluat...Objective To modify the double bonds and hydroxyl groups of marine natural product brefeldin A(1),derivatives 2~10 were prepared and their inhibitory activities against lung adenocarcinoma epithelial A549 were evaluated.Methods The cytotoxic activities of all derivatives(2~10)against lung adenocarcinoma epithelial A549 were evaluated using MTT assay.Conclusion Derivatives 2~7 and 8~10 were designed and synthesized via introducing sulfur atoms and acetylation into the structure of 1,respectively.All derivatives can inhibit the growth of A549 cells below 1μmol/L,among which derivatives 5 and 9 exhibited significant activities,with IC50 values of 40 nM and 98 nM respectively,which can be used as potential anticancer lead compounds.展开更多
Seven novel linear polyketides,talaketides A-G(1-7),were isolated from the rice media cultures of the mangrove sed-iment-derived fungus Talaromyces sp.SCSIO 41027.Among these,talaketides A-E(1-5)represented unpreceden...Seven novel linear polyketides,talaketides A-G(1-7),were isolated from the rice media cultures of the mangrove sed-iment-derived fungus Talaromyces sp.SCSIO 41027.Among these,talaketides A-E(1-5)represented unprecedented unsaturated lin-ear polyketides with an epoxy ring structure.The structures,including absolute configurations of these compounds,were elucidated through detailed analyses of nuclear magnetic resonance(NMR)and high-resolution mass spectrometry(HR-MS)data,as well as elec-tronic custom distributors(ECD)calculations.In the cytotoxicity screening against prostate cancer cell lines,talaketide E(5)demon-strated a dose-dependent inhibitory effect on prostate cancer PC-3 cell lines,with an IC50 value of 14.44 μmol·L-1.Moreover,com-pound 5 significantly inhibited the cloning formation of PC-3 cell lines and arrested the cell cycle in S-phase,ultimately inducing ap-optosis.These findings indicate that compound 5 may serve as a promising lead compound for the development of a potential treat-ment for prostate cancer.展开更多
The ruthenium multi-substituted polyoxotungstate,K_(7)[Si W_(9)O_(37)Ru_(4)(H_(2)O)_(3)Cl_(3)]·15H_(2)O(S1),was synthesized by a conventional aqueous solution containing the trilacunary Keggin-anionsβ-Na_(9)HSi ...The ruthenium multi-substituted polyoxotungstate,K_(7)[Si W_(9)O_(37)Ru_(4)(H_(2)O)_(3)Cl_(3)]·15H_(2)O(S1),was synthesized by a conventional aqueous solution containing the trilacunary Keggin-anionsβ-Na_(9)HSi W_(9)O_(34)·12H_(2)O(S2)and Ru Cl_(3)·n H_(2)O(S3).Compound S1 was charac‐terized by elemental analysis,energy-dispersive X-ray spectroscopy(EDS),thermogravimetric analysis(TG),infrared spectroscopy(IR),uliraviolet visible absorption spectroscopy(UV/Vis)and X-ray photoelectron spectroscopy(XPS).The cytotoxicitycy of S1 was tested in C33A(human cervical cancer),DLD-1(human colon cancer),Hep G2(human liver cancer)and human normal embryonic lung fibroblasts cell(MRC-5).And the viability of these treated cells was evaluated by MTT(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bro‐mide)assay.To explore the mode of cell death induced by S1,morphological study of DNA damage and apoptosis assays were conducted.These analyses revealed that S1 exerted its cytotoxic effect in a dose-dependent manner,primarily triggering apoptotic cell death.Cell cycle analysis by flow cytometry indicated that compound S1 caused cell cycle arrest and accumulated cells in S phase.展开更多
In this study,we successfully synthesized Er_(2)TiO_(5)@Ag nanocomposites(NCPs) using the ultrasonicmediated sol-gel technique to create a multifunctional material with enhanced photocatalytic and antibacterial proper...In this study,we successfully synthesized Er_(2)TiO_(5)@Ag nanocomposites(NCPs) using the ultrasonicmediated sol-gel technique to create a multifunctional material with enhanced photocatalytic and antibacterial properties.The visible light photocatalytic activities of Er_(2)TiO_(5) nanoparticles(NPs) and Er_(2)TiO_(5)@Ag NCPs were systematically evaluated under various conditions,including different concentrations of Basic Blue 41(BB 41) dye and photocatalyst.The results reveal a remarkable improvement in the photocatalytic degradation efficiency of Er_(2)TiO_(5)@Ag NCPs(95%) compared to Er_(2)TiO_(5) NPs(80%).Furthermore,the antibacterial efficacy of Er_(2)TiO_(5) NPs and Er2TiO_5@Ag NCPs were extensively examined against Gram-positive and Gram-negative bacteria.Notably,Er_(2)TiO_(5)@Ag NCPs exhibit significantly higher minimum bactericidal concentration(MBC) values compared to Er_(2)TiO_(5) NPs.The antibacterial effect of Er_(2)TiO_(5)@Ag NCPs is particularly pronounced against S.aureus and Pseudomonas aeruginosa,while demonstrating moderate effects on Escherichia coli and Enterococcus faecalis.To assess the biocompatibility of the synthesized materials,we investigated their internalization by MCF-7 cells.Encouragingly,both Er_(2)TiO_(5) NPs and Er_(2)TiO_(5)@Ag NCPs are found to be effectively internalized by the cells,suggesting their potential application in biomedical fields.Intriguingly,our study unveils the exceptional potential of Er_(2)TiO_(5)@Ag NCPs as a dual-action solution,simultaneously possessing enhanced photocatalytic efficiency and potent antibacterial properties.This multifunctional nanocomposite not only outperforms Er_(2)TiO_(5) and Ag but also paves the way for innovative applications in sustainable environmental remediation and advanced biomedical technologies,promising a brighter and cleaner future.展开更多
Cancer is one of the deadliest diseases in developing countries. In recent years, natural plant-based compounds have been used in the search for drugs to combat numerous diseases, including cancer. In this study, we e...Cancer is one of the deadliest diseases in developing countries. In recent years, natural plant-based compounds have been used in the search for drugs to combat numerous diseases, including cancer. In this study, we evaluate the cytotoxic properties of paanfo tiben 1 and paanfo tiben 2, two traditional herbal formulations from Burkina Faso used in the treatment of cancer in Burkina Faso. To this end, the recipes were infused and freeze-dried. The dry extracts obtained were used to determine total phenolics and flavonoids content, assess antioxidant activity using the DPPH, ABTS and FRAP methods, evaluate anti-inflammatory properties by inhibiting 15-LOX, COX 1 and 2, and assess cytotoxic activity on HeLa cervical cancer and HePG2 liver cancer cell lines using the MTT test. The paanfo tiben 1 recipe showed the highest levels of total phenolics and flavonoids, as well as the best antioxidant activities, with IC50 values of 21.020 ± 0.6 µg/ml and 22.94 ± 0.57 µg/ml for DPPH and ABTS, and 165.15 mM EAA/mg dry extract for FRAP. It also exhibited the best cytotoxic activity with IC50 values of 112.02 ± 0.025 µg/ml on HeLa cells and 80.67 ± 6.08 µg/ml on HepG2 cells. On the other hand, paanfo tiben 2 exhibited the best anti-inflammatory activities through inhibition of 15-LOX and COX 1, with inhibition percentages at 100 µg/ml of 32.523% and 24.717 % respectively. These results could justify the traditional use of these two recipes by traditional health practitioners in the treatment of cancer sufferers in Burkina Faso.展开更多
基金supported by the National Natural Science Foundation of China(Grant No.82274034)the Peking University Medicine plus X Pilot Program-Platform Construction Project(Grant No.2024YXXLHPT004).
文摘Ovarian cancer remains a leading cause of gynecological cancer mortality1,and patients with advanced stage ovarian cancer frequently develop malignant ascites that foster immunosuppressive microenvironments and therapeutic resistance2,3.Although ascites have traditionally been considered detrimental,we report a paradoxical role in which they enhance the cytotoxicity ofγδT cells—a unique T cell subset that can be allogenically transferred for cancer treatment4,5—toward ovarian cancer.
基金supported by the Qi-Huang Chief Scientist Project of the National Administration of Traditional Chinese Medicine(2020).
文摘Phytochemical study of the n-BuOH extract of Ilex asprella resulted in the discovery of ten new pentacyclic triterpenoid glycosides,comprising nine ursane-type glycosides(1-9)and one oleanane-type glycoside(10),along with seven known compounds(11-17).Compound 1 is the first reported 19,22-epoxy ursane triterpenoid glycoside,whereas 4 and 5 are rare examples of ursane triterpenoid glycosides containing a 28,19-lactone group.The structural characterization of these compounds was achieved using spectroscopic and chemical techniques,as well as single-crystal X-ray analysis.Compounds 7,12,15,and 17 exhibited moderate cytotoxic activities against H1975 and HCC827 cancer cells.
基金supported by the National Key Research and Development Program of China(No.2022YFC2303100)the National Natural Science Foundation of China(Nos.32022002 and 21977113).
文摘(±)-Penicithrones A–D(1a/1b–4a/4b),four novel pairs of anthrone–cyclopentenone heterodimers characterized by a distinctive bridged 6/6/6−5 tetracyclic core skeleton,together with three previously identified compounds(5–7),were isolated from the crude extract of the mangrove-derived fungus Penicillium sp.,guided by heteronuclear single quantum correlation(HSQC)-based small molecule accurate recognition technology(SMART 2.0)and liquid chromatography-tandem mass spectrometry(LC-MS/MS)-based molecular networking.The structural elucidation of new compounds was accomplished through comprehensive spectroscopic analysis,and their absolute configurations were determined using DP4+^(13)C nuclear magnetic resonance(NMR)calculations and electronic circular dichroism(ECD)calculations.Compounds 1a/1b–4a/4b demonstrated moderate cytotoxicity against three human cancer cell lines HeLa,HCT116 and MCF-7 with half maximal inhibitory concentration(IC50)values ranging from 15.95±1.64 to 28.56±2.59μmol·L–1.
文摘Antipalu is a phytomedicinal medicinal beverage that is popular in the District of Abidjan, particularly for the treatment of malaria. However, Antipalu could present potential health effects on patients, and few toxicological studies have been conducted before its use. In order to determine the cytotoxicity of Antipalu, two complementary tests, LDH activity and the MTT cell proliferation assay, were used using Vero cells. Vero cells were exposed to increasing concentrations of Antipalu and incubated for 24, 48 and 72 hours. In addition, forty (40) rats distributed randomly into 4 groups, including 10 animals per group (5 males and 5 females) were used for the potential hepatoxic effects. Animals in group 1 received distilled water and were used as a control group. On the other hand, Lot I, II and III received by gavage a volume of the Antipalu extract corresponding to 1 ml/100 g of body weight at 200 mg/kg, 400 mg/kg, 800 mg/kg, respectively. The extract was administered daily at the same time for 28 days and serum was collected once a week to evaluate hepatic biochemical markers. After 28 days of study, all rats were euthanized by an overdose of ether and the liver of the rats was removed for gross morphological and histopathological analysis. The results of the cell supernatant assay showed an increasing extracellular LDH enzyme activity with lethal concentrations at 10% and 50% (LC10 = 111 µg/mL and LC50 = 555 µg/mL, respectively). In addition, the MTT assay showed a decrease in mitochondrial activity and thus cell proliferation after 24, 48 and 72 H of incubation. Our study showed that Antipalu caused alterations in the plasma membranes of the cells, resulting in the release of lactase dehydrogenase (LDH) into the external environment and a decrease in the mitochondrial activity of the Vero cells. The biochemical parameters ALT, ASAT, ALPs, and GGT showed no significant change (P > 0.05) in the group of treated rats compared to the controls. However, these variations were moderate and transient, with values remaining almost within their standard limits. Microscopic observations of liver tissue sections from rats treated with the Antipalu showed no lesions, edema and necrosis. These results suggest that the Antipalu did not interfere with the functioning or alter the integrity of the liver.
基金National Natural Science Foundation of China(U22A20380,82173706,82104028)Fundamental Research Funds for the Central Universities(2024BRA018)financially supported this project.
文摘Five novel emestrin-type epipolythiodioxopiperazines(ETPs),prenylemestrins C-G(1-5),along with two known ETPs,prenylemestrin A(6)and prenylemestrin B(7),were obtained from Aspergillus nidulans.Their structures were characterized by spectroscopic data,X-ray crystallographic data,ECD comparisons and calculations.Prenylemestrins C-G(1-5)represent a rare class of ETPs,characterized by a 2,5-dithia-7,9-diazabicyclo[4.2.2]decane-8,10-dione core involving a hemiterpene moiety.Notably,compound 6 exhibited moderate cytotoxicity,inducing G2/M cell cycle arrest and apoptosis of L1210 cells by regulating the PI3K/AKT signaling pathway and mitochondrial apoptotic mechanisms.
文摘The interest in using the Datura stramonium plant is due to its natural products,which are used in many pharmaceutical industries.The objective of the current study was to assess the therapeutic and cytotoxic effects of the D.stramonium plant on two types of human cancer cell models(MCF7 and HT29)in vitro.A soxhlet apparatus was used to obtain methanolic extract from dried plant leaves.The recovered crude,after the solvent had evaporated,was then dispersed at varied concentrations of extract 100,50,20,and 0.0µg/mL and tested to see how the cells responded.Also,the cancer-testis antigen(CTA)gene transcription in the two cell types exposed to the plant extract was examined using a semi-quantitative real-time polymerase chain reaction.Gas chromatography–mass spectrometry(GC-MS)results produced the significant main metabolites Nonanoic acid,Tropine N-Oxide,3,6-Ditigloyloxy-7-hydroxytropane,Hexadecanoic acid,2-Pentadecanone,6,10,14-trimethyl-,Carvenone,methyl ester,Phytol,Aposcopolamine,Hyoscyamine,4,8,12,16-Tetramethylheptadecan-4-olide,Scopolamine,Alpha.-Tocospiro A,1,2-Cycloheptanedione,3,3,7,7-tetramethyl-,dihydrazone,Campesterol,Stigmasterol,Gamma-Sitosterol and dl-.alpha.-Tocopherol.The results showed that the two types of cell lines impacted by D.stramonium extract,through untreated type 1 cells(MCF7)gave a highly significant transcription according to all applicable genes.All implemented analyses cleared the strong genetic impacts of Datura extract on cancer cells’genomes.TGIF2LY and C2orf63 transcript accumulation were also significantly elevated when exposed to plant extract at a level of 50µg/mL in cell line type 2(HT29),but TGIF2LY and P53 had the lowest relative expression at a level of 100µg/mL when treated the same cell line type.
基金sponsored by National Natural Science Foundation of China(81800703)Postdoctoral Fellowship Program of China Postdoctoral Science Foundation(GZC20231088)+8 种基金Beijing Nova Program(Z201100006820117 and 20220484181)Beijing Municipal Natural Science Foundation(7184252)the Fundamental Research Funds for the Central Universitiesthe Fundamental Research Funds for the Central Universities(BMU2021MX013)Peking University Clinical Scientist Training Program(BMU2023PYJH022)Peking University Medicine Seed Fund for Interdisciplinary ResearchChina Endocrine and Metabolism Young Scientific Talent Research Project(2022-N-02-01)China Diabetes Young Scientific Talent Research ProjectBethune-Merck Diabetes Research Fund of Bethune Charitable Foundation。
文摘Maternal consumption of a high-fat diet has been linked to increased risks of obesity and impaired glucose metabolism in offspring.However,the precise epigenetic mechanisms governing these intergenerational effects,particularly during the early stages of offspring development,remain poorly understood.In this study,female C57BL/6J mice were randomly assigned to either a high-fat diet or normal chow diet throughout gestation and lactation.Methylated DNA immunoprecipitation(MeDIP)coupled with microarray analysis was employed to identify differentially methylated genes in the livers of offspring at weaning age.We found that maternal high-fat diet feeding predisposes offspring to obesity and impaired glucose metabolism as early as the weaning period.DNA methylation profile analysis unveiled a significant enrichment of differentially methylated genes within the natural killer(NK)cell-mediated cytotoxicity pathway.MeDIP-PCR validated reduced methylation levels of specific genes within this pathway,including tumour necrosis factorα(TNF-α),phosphoinositide 3-kinase(PI3K),and SHC adaptor protein 1(SHC1).Consistently,the expressions of TNF-α,PI3K,and SHC1 were significantly upregulated,accompanied by elevated serum TNF-αand interleukin-6(IL-6)levels in offspring from dams fed with high-fat diet.Moreover,we assessed the expressions of genes associated with NK cell activities,uncovering a notable rise in hepatic granzyme B levels and a trend towards increased CD107a expression in offspring from dams fed a high-fat diet.In addition,methylation levels of TNF-α,PI3K,and SHC1 promoters were inversely correlated with glucose response during glucose tolerance testing.In conclusion,our findings underscore the critical role of the NK cell-mediated cytotoxicity signaling pathway in mediating DNA methylation patterns,thereby contributing to the programming effects of maternal high-fat diet consumption on offspring glucose metabolism as early as the weaning period.
文摘K−Na co-doped δ-MnO_(2)(KNMOH)nanoflowers were synthesized,and their cytotoxic effects against HeLa cervical cancer cells were evaluated.The KNMOH exhibited significant dose-and time-dependent cytotoxicity at concentrations of 50 and 100μg/mL.After 24 h of incubation treatment,cell viability decreased to(36.8±6.5)% and(33.4±6.4)%at 50 and 100μg/mL,respectively.With extended exposure to 48 h,cell viability was(45.2±2.3)%and(32.3±2.8)%at the same concentrations.Phase-contrast microscopy revealed characteristic morphological changes including cell shrinkage and membrane blebbing formation,indicative of cell death.These findings demonstrate the potential of KNMOH nanoflowers as a cytotoxic agent for cervical cancer applications and provide a foundation for further mechanistic studies.
基金funded by Silesian University of Technology,no.07/020/BKM24/0104.
文摘Plasma electrolytic oxidation is a well-known technique for surface modification of biomedical magnesium alloys,with good corrosion protection and the ability to produce biocompatible and bioactive coatings.In this study,calcium-phosphate coatings were produced on WE43 magnesium alloy for use,as orthopedic implants.Coating formation was prepared using different oxidation parameters with various duty ratios(DR)of 15,25 and 50%and current ratios(R)-2 or 1.6.Application of R with excess cathodic current(R>1)in processes with DR≥25%allowed attaining the soft-sparking regime(SSR)that resulted in thicker oxide coatings with higher degree of crystallinity compared to the films obtained without SSR.The results of the corrosion tests contributed to a noticeable improvement in the corrosion resistance of the magnesium alloy.Optimization of the oxidation parameters allowed the selection of the variants with the most favorable degradation behavior over the tested immersion period,indicating a successful modification of the magnesium alloy surface to obtain an implant biomaterial capable of providing controlled degradation.Furthermore,biological evaluation of the produced coatings showed that the proposed surface modifications significantly reduced the cytotoxic effects observed in direct contact with the material while still maintaining the cell proliferation-promoting effects of the material eluents.
基金supported by the Natural Science Funds for Distinguished Young Scholars of Anhui Province(No. 2108085J18)the National Natural Science Foundation of China (Nos. 32011540382 and 32102272)。
文摘Chemical investigations of the termite-associated Streptomyces tanashiensis BYF-112 resulted in the discovery of four novel alkaloid derivatives: vegfrecines A and B(1 and 2), exfoliazone A(3), and venezueline H(7), in addition to nine known metabolites(4-6, 8-13). The structures of these compounds were elucidated through comprehensive spectroscopic analysis and comparison with existing literature data. Antibacterial assays revealed that viridomycin A(11) exhibited potent antibacterial activity against Staphylococcus aureus, with a zone of inhibition(ZOI) of 12.67 mm, in comparison to a ZOI of 17.67 mm for the positive control gentamicin sulfate. Viridomycin A(11) showed moderate activity against Micrococcus tetragenus and Pseudomonas syringae pv. actinidae, with ZOI values of 15.50 and 14.33 mm, respectively, which were inferior to those of gentamicin sulfate(34.67 and 24.00mm). Viridomycin F(12) also exhibited moderate antibacterial effects against S. aureus, M. tetragenus, and P. syringae pv. actinidae,with ZOI values of 8.33, 16.50, and 10.83 mm, respectively. Cytotoxicity assays demonstrated that viridobruunine A(5), exfoliazone(6), viridomycin A(11), and X-14881E(13) exhibited significant cytotoxicity against human malignant melanoma(A375), ovarian cancer(SKOV-3), and gastric cancer(MGC-803) cell lines, with IC_(50) values ranging from 4.61 to 19.28 μmol·L^(-1). Furthermore, bioinformatic analysis of the complete genome of S. tanashiensis suggested a putative biosynthetic gene cluster(BGC) responsible for the production of compounds 1-12. These findings indicate that the secondary metabolites of insect-associated S. tanashiensis BYF-112hold promise as potential sources of novel antibacterial and anticancer agents.
基金supported by the National Natural Science Foundation of China(Nos.22077041,81803847 and 31770380).
文摘A phytochemical investigation of the whole plant of Parasenecio rubescens(S.Moore)Y.L.Chen yielded 14 previ-ously undescribed,highly oxidized bisabolane-type sesquiterpenoids,named pararunines L-Y,along with one known oplopane-type sesquiterpenoid.The structural elucidation of these compounds was accomplished through comprehensive spectroscopic analysis,in-cluding nuclear magnetic resonance(NMR)and high-resolution electrospray ionization mass spectrometry(HR-ESI-MS)techniques.Motivated by traditional uses and previous studies on this genus,all isolated compounds were subjected to in vitro cytotoxicity assays against four human cancer cell lines(MCF-7,Hela,HCT116,and HT-29).Considering that the reported chemical constituents of nu-merous other species within this genus primarily consist of eremophilane-type sesquiterpenoids,our findings not only expand the struc-tural diversity of bisabolane-type sesquiterpenoids but also contribute valuable scientific evidence to the chemotaxonomy of this genus.
基金national financial support from the National Key R&D Program of China(No.2019YFA0905700)the National Natural Science Foundation of China(Nos.82173703 and 81874293)the Major Basic Research Program of Shandong Provincial Natural Science Foundation(No.ZR2019ZD26)。
文摘Two distinctive rearranged 19-nor-7,8-seco-labdane diterpenoids(1 and 2)with a novel tetracyclo[5.2.1.0^(2,5.)0^(4,10)]decane skeleton,a derivative of the open tetrahydrofuran ring(7),three dimeric compounds(8-10),and four revised homologs(3-6)were obtained from Chinese liverwort Pallavicinia ambigua.Their structures were identified via combined analysis of their spectroscopic data,single-crystal X-ray diffraction patterns,and ECD calculations.The light-driven conversion of compound 5 to compounds 1-4 demonstrated that photochemically induced postmodification involved in biosynthesis is an important way to diversify natural structures.A preliminary cytotoxicity assay revealed that compound 5 showed significant inhibition in the human prostate cancer(PC-3)cell line via an apoptotic pathway.
基金supported by the Key Research Project of Higher Education Institution in Henan Province (No.24A360015)the Scientific and Technological Key Project in Henan Province (No. 192102310438)+1 种基金the Program for Innovative Research Team (in Science and Technology) in University of Henan Province(No. 24IRTSTHN039)the Research Project on Chinese Medicine Science in Henan Province (No. 20-21ZY1039)。
文摘Eight novel arylnaphthalide lactone lignans, designated as diphylignan A-H(1-8), and a new dibenzyltyrolactone lignan, designated as diphylignan I(9), were isolated from the roots and rhizomes of Diphylleia sinensis, along with two additional novel natural products(11 and 14) and four known metabolites(10, 12, 13, 15). The structural and stereochemical characterization of these compounds was accomplished using NMR spectroscopy and electronic circular dichroism(ECD) analysis. The cytotoxic activities of all isolated compounds were assessed against A-549 and SMMC-7721 cell lines. Notably, compound 2 demonstrated the most significant cytotoxicity, with IC_(50) values of 10.27 and 11.58 μmol·L^(-1) against A-549 and SMMC-7721 cell lines, respectively, exhibiting greater potency than the positive control, cisplatin.
基金supported by the National Natural Science Foundation of China(No.82003629)the Natural Science Foundation of Chongqing(No.cstc2021jcyj-msxmX0975)the Open Project of State Key Laboratory of Natural Medicines(No.SKLNMKF202304).
文摘Citrinsorbicillin A(1),a novel homotrimeric sorbicillinoid,along with two new monomers citrinsor-bicillins B(2)and C(3),were isolated from the Coptis chinensis endophyte Trichoderma citrinoviride HT-9 by liquid chromatograph mass spectrometer(LC-MS)-guided strategy.1 was the first trimeric-example from terrestrial fungi,which possessed a unique carbon skeleton with two bicyclo[2.2.2]octanedione ring connected through an enolated carbon forming by[4+2]cycloaddition.Their structures were elucidated by spectroscopic analysis and X-ray diffraction.1 exhibited moderate cytotoxicity against human colon cancer HT29 cells,and it induced significant cell cycle arrest by reducing the protein expression of cyclin D1.
基金supported by the National Natural Science Foundation of China(No.82173713)the Natural Science Foundation of Hunan Province(Nos.2021JJ41000 and2021JJ40812)+2 种基金the Key Research and Development Program of Hunan Province(No.2022SK2031)the Project of Hunan Administration of Traditional Chinese Medicine(No.B2023059)the Postgraduate Scientific Research Innovation Project of Hunan Province(No.CX20230323)。
文摘Four novel macrocyclic trichothecenes,termed mytoxins D-G(1-4),along with four known analogs(5-8),were isolated from the ethyl acetate extract of fermented rice inoculated with the fungus Myrothecium verrucaria PA57.Each compound features a tricyclic 12,13-epoxytrichothec-9-ene(EPT)core.Notably,mytoxin G(4)represents the first instance of a macrocyclic trichothecene incorporating a glucosyl unit within the trichothecene structure.The structures of the newly identified compounds were elucidated through comprehensive spectroscopic analysis combined with quantum chemical calculations.All isolated compounds demonstrated cytotoxic activity against the CAL27 and HCT116 cell lines,which are models for human oral squamous cell carcinoma and colorectal cancer,respectively.Specifically,mytoxin D(1)and mytoxin F(3)exhibited pronounced cytotoxic effects against both cancer cell lines,with IC_(50)values ranging from 3 to 6 nmol·L^(-1).Moreover,compounds 1 and 3 were found to induce apoptosis in HCT116 cells by activating caspase-3.
文摘Objective To modify the double bonds and hydroxyl groups of marine natural product brefeldin A(1),derivatives 2~10 were prepared and their inhibitory activities against lung adenocarcinoma epithelial A549 were evaluated.Methods The cytotoxic activities of all derivatives(2~10)against lung adenocarcinoma epithelial A549 were evaluated using MTT assay.Conclusion Derivatives 2~7 and 8~10 were designed and synthesized via introducing sulfur atoms and acetylation into the structure of 1,respectively.All derivatives can inhibit the growth of A549 cells below 1μmol/L,among which derivatives 5 and 9 exhibited significant activities,with IC50 values of 40 nM and 98 nM respectively,which can be used as potential anticancer lead compounds.
基金supported by the Key-Area Research and Development Program of Guangdong Province(No.2023B1111050008)the National Natural Science Foundation of China(Nos.U23A20528,U20A20101)+1 种基金Guangdong Local Innovation Team Program(No.2019BT02Y262)the Postdoctoral Fellowship Program of CPSF(No.GZC20232777).
文摘Seven novel linear polyketides,talaketides A-G(1-7),were isolated from the rice media cultures of the mangrove sed-iment-derived fungus Talaromyces sp.SCSIO 41027.Among these,talaketides A-E(1-5)represented unprecedented unsaturated lin-ear polyketides with an epoxy ring structure.The structures,including absolute configurations of these compounds,were elucidated through detailed analyses of nuclear magnetic resonance(NMR)and high-resolution mass spectrometry(HR-MS)data,as well as elec-tronic custom distributors(ECD)calculations.In the cytotoxicity screening against prostate cancer cell lines,talaketide E(5)demon-strated a dose-dependent inhibitory effect on prostate cancer PC-3 cell lines,with an IC50 value of 14.44 μmol·L-1.Moreover,com-pound 5 significantly inhibited the cloning formation of PC-3 cell lines and arrested the cell cycle in S-phase,ultimately inducing ap-optosis.These findings indicate that compound 5 may serve as a promising lead compound for the development of a potential treat-ment for prostate cancer.
基金Supported by the National Natural Science Foundation of China (21701120)the Science and Technology Innovation Project of Colleges and Universities in Shanxi Province (2020L0334)the Innovation and Entrepreneurship Training Program for College Students in Shanxi Province(20240778)。
文摘The ruthenium multi-substituted polyoxotungstate,K_(7)[Si W_(9)O_(37)Ru_(4)(H_(2)O)_(3)Cl_(3)]·15H_(2)O(S1),was synthesized by a conventional aqueous solution containing the trilacunary Keggin-anionsβ-Na_(9)HSi W_(9)O_(34)·12H_(2)O(S2)and Ru Cl_(3)·n H_(2)O(S3).Compound S1 was charac‐terized by elemental analysis,energy-dispersive X-ray spectroscopy(EDS),thermogravimetric analysis(TG),infrared spectroscopy(IR),uliraviolet visible absorption spectroscopy(UV/Vis)and X-ray photoelectron spectroscopy(XPS).The cytotoxicitycy of S1 was tested in C33A(human cervical cancer),DLD-1(human colon cancer),Hep G2(human liver cancer)and human normal embryonic lung fibroblasts cell(MRC-5).And the viability of these treated cells was evaluated by MTT(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bro‐mide)assay.To explore the mode of cell death induced by S1,morphological study of DNA damage and apoptosis assays were conducted.These analyses revealed that S1 exerted its cytotoxic effect in a dose-dependent manner,primarily triggering apoptotic cell death.Cell cycle analysis by flow cytometry indicated that compound S1 caused cell cycle arrest and accumulated cells in S phase.
基金Project supported by the Kashan University of Medical Sciences, Kashan,Iran (99181)Council of University of Medical Science,Kashan for providing financial support to this work。
文摘In this study,we successfully synthesized Er_(2)TiO_(5)@Ag nanocomposites(NCPs) using the ultrasonicmediated sol-gel technique to create a multifunctional material with enhanced photocatalytic and antibacterial properties.The visible light photocatalytic activities of Er_(2)TiO_(5) nanoparticles(NPs) and Er_(2)TiO_(5)@Ag NCPs were systematically evaluated under various conditions,including different concentrations of Basic Blue 41(BB 41) dye and photocatalyst.The results reveal a remarkable improvement in the photocatalytic degradation efficiency of Er_(2)TiO_(5)@Ag NCPs(95%) compared to Er_(2)TiO_(5) NPs(80%).Furthermore,the antibacterial efficacy of Er_(2)TiO_(5) NPs and Er2TiO_5@Ag NCPs were extensively examined against Gram-positive and Gram-negative bacteria.Notably,Er_(2)TiO_(5)@Ag NCPs exhibit significantly higher minimum bactericidal concentration(MBC) values compared to Er_(2)TiO_(5) NPs.The antibacterial effect of Er_(2)TiO_(5)@Ag NCPs is particularly pronounced against S.aureus and Pseudomonas aeruginosa,while demonstrating moderate effects on Escherichia coli and Enterococcus faecalis.To assess the biocompatibility of the synthesized materials,we investigated their internalization by MCF-7 cells.Encouragingly,both Er_(2)TiO_(5) NPs and Er_(2)TiO_(5)@Ag NCPs are found to be effectively internalized by the cells,suggesting their potential application in biomedical fields.Intriguingly,our study unveils the exceptional potential of Er_(2)TiO_(5)@Ag NCPs as a dual-action solution,simultaneously possessing enhanced photocatalytic efficiency and potent antibacterial properties.This multifunctional nanocomposite not only outperforms Er_(2)TiO_(5) and Ag but also paves the way for innovative applications in sustainable environmental remediation and advanced biomedical technologies,promising a brighter and cleaner future.
文摘Cancer is one of the deadliest diseases in developing countries. In recent years, natural plant-based compounds have been used in the search for drugs to combat numerous diseases, including cancer. In this study, we evaluate the cytotoxic properties of paanfo tiben 1 and paanfo tiben 2, two traditional herbal formulations from Burkina Faso used in the treatment of cancer in Burkina Faso. To this end, the recipes were infused and freeze-dried. The dry extracts obtained were used to determine total phenolics and flavonoids content, assess antioxidant activity using the DPPH, ABTS and FRAP methods, evaluate anti-inflammatory properties by inhibiting 15-LOX, COX 1 and 2, and assess cytotoxic activity on HeLa cervical cancer and HePG2 liver cancer cell lines using the MTT test. The paanfo tiben 1 recipe showed the highest levels of total phenolics and flavonoids, as well as the best antioxidant activities, with IC50 values of 21.020 ± 0.6 µg/ml and 22.94 ± 0.57 µg/ml for DPPH and ABTS, and 165.15 mM EAA/mg dry extract for FRAP. It also exhibited the best cytotoxic activity with IC50 values of 112.02 ± 0.025 µg/ml on HeLa cells and 80.67 ± 6.08 µg/ml on HepG2 cells. On the other hand, paanfo tiben 2 exhibited the best anti-inflammatory activities through inhibition of 15-LOX and COX 1, with inhibition percentages at 100 µg/ml of 32.523% and 24.717 % respectively. These results could justify the traditional use of these two recipes by traditional health practitioners in the treatment of cancer sufferers in Burkina Faso.
