AIM： To investigate the anti-fibrosis effect of the tissue transglutarninase （tTG） specific inhibitor cystarnine on liver fibrosis. METHODS： Sixty-eight male Sprague Dawley rats were divided into three groups： no...AIM： To investigate the anti-fibrosis effect of the tissue transglutarninase （tTG） specific inhibitor cystarnine on liver fibrosis. METHODS： Sixty-eight male Sprague Dawley rats were divided into three groups： normal control, liver fibrosis control and cystamine-treated group. Liver fibrosis was induced by intraperitoneal injection of carbon tetrachloride （CCl4）, and Cystarnine was administrated by intraperitoneal injection starting 2 d before the first administration of CCl4. Animals in each group were further divided into 2 subgroups according to two time points of 4 wk and 8 wk after treatment. Hepatic function, pathological evaluation （semi-quantitative scoring system, SSS） and liver hydroxyproline （Hyp） content were examined. Real-time PCR was used to detect the expression of tTG, smooth muscle alpha actin （α-SMA）, tissue inhibitor of metalloproteinase 1 （TIMP-1） and collagen-1 mRNA. The expressions of tTG and α-SMA protein were detected by Western Blotting. RESULTS： Eight weeks after treatment, the SSS score of liver was significantly less in the cystamine group than that in the fibrosis control group （P 〈 0.01）. The levels of alanine arninotransferase （ALT） and total bile acid （TBA） at the 4 wk and 8 wk time points were decreased in the cystamine group compared with those in fibrosis controls （P 〈 0.01）. Liver hydroxyproline content at the 4 wk and 8 wk time points showed a substantial reduction in the cystamine group compared to fibrosis controls （P 〈 0.01）. The expression of tTG, α-SMA, collagen-1, TIMP-1 mRNA and tTG, as well as α-SMA protein was downregulated in the cystamine group compared to fibrosis controls. CONCLUSION： Cystamine can ameliorate CCl4 induced liver fibrosis and protect hepatic function. The possible mechanism is related to the reduced synthesis of the extracellular matrix （ECM） caused by the inhibition of hepatic stellate cell activation and decreased expression of TIMP-1.展开更多
Metal foils for Raman scattering were prepared by HNO_3 etching method. These foils werefound to exhibit a strong SERS effect and excellent thermal stability. SERS spectra of cystaminehave been taken to illustrate the...Metal foils for Raman scattering were prepared by HNO_3 etching method. These foils werefound to exhibit a strong SERS effect and excellent thermal stability. SERS spectra of cystaminehave been taken to illustrate the usefulness of this sample preparation method in the studies ofchemisorption. The ring opening reactions of an epoxy compound have been observed on the topof chemisorbed cystamine on silver foils.展开更多
基金Supported by National Natural Science Foundation of China,No. 30571825
文摘AIM： To investigate the anti-fibrosis effect of the tissue transglutarninase （tTG） specific inhibitor cystarnine on liver fibrosis. METHODS： Sixty-eight male Sprague Dawley rats were divided into three groups： normal control, liver fibrosis control and cystamine-treated group. Liver fibrosis was induced by intraperitoneal injection of carbon tetrachloride （CCl4）, and Cystarnine was administrated by intraperitoneal injection starting 2 d before the first administration of CCl4. Animals in each group were further divided into 2 subgroups according to two time points of 4 wk and 8 wk after treatment. Hepatic function, pathological evaluation （semi-quantitative scoring system, SSS） and liver hydroxyproline （Hyp） content were examined. Real-time PCR was used to detect the expression of tTG, smooth muscle alpha actin （α-SMA）, tissue inhibitor of metalloproteinase 1 （TIMP-1） and collagen-1 mRNA. The expressions of tTG and α-SMA protein were detected by Western Blotting. RESULTS： Eight weeks after treatment, the SSS score of liver was significantly less in the cystamine group than that in the fibrosis control group （P 〈 0.01）. The levels of alanine arninotransferase （ALT） and total bile acid （TBA） at the 4 wk and 8 wk time points were decreased in the cystamine group compared with those in fibrosis controls （P 〈 0.01）. Liver hydroxyproline content at the 4 wk and 8 wk time points showed a substantial reduction in the cystamine group compared to fibrosis controls （P 〈 0.01）. The expression of tTG, α-SMA, collagen-1, TIMP-1 mRNA and tTG, as well as α-SMA protein was downregulated in the cystamine group compared to fibrosis controls. CONCLUSION： Cystamine can ameliorate CCl4 induced liver fibrosis and protect hepatic function. The possible mechanism is related to the reduced synthesis of the extracellular matrix （ECM） caused by the inhibition of hepatic stellate cell activation and decreased expression of TIMP-1.
文摘Metal foils for Raman scattering were prepared by HNO_3 etching method. These foils werefound to exhibit a strong SERS effect and excellent thermal stability. SERS spectra of cystaminehave been taken to illustrate the usefulness of this sample preparation method in the studies ofchemisorption. The ring opening reactions of an epoxy compound have been observed on the topof chemisorbed cystamine on silver foils.
