Cypripedium tibeticum is a threatened orchid which efficient conservation requires knowledge of its extent and structure of genetic variation. Using two chloroplast DNA fragments(rps16 and trnL-F), we analyzed 157 ind...Cypripedium tibeticum is a threatened orchid which efficient conservation requires knowledge of its extent and structure of genetic variation. Using two chloroplast DNA fragments(rps16 and trnL-F), we analyzed 157 individuals from 9 populations representing the species range in China. Seven haplotypes were identified. C. tibeticum had high total genetic diversity(H_T=0.80) with major contribution to this diversity made by among-population component(G_ST=0.64, Ф_ST= 0.86). However, despite high population differentiation there was no clear phylogeographic structure. The populations CY and DC made the greatest contribution to the total gene diversity as well as allelic richness. The possible mechanisms and implications of these findings for conservation of the species are discussed.展开更多
A new phenanthraquinone, cypripediquinone A, was isolated from the rhizome of Cypripedium macranthum ( Orchidaceae ) and its structure was established as 2,4,7-trimethoxy-9, 10- phenanthraquinone mainly by spectroscop...A new phenanthraquinone, cypripediquinone A, was isolated from the rhizome of Cypripedium macranthum ( Orchidaceae ) and its structure was established as 2,4,7-trimethoxy-9, 10- phenanthraquinone mainly by spectroscopic analysis.展开更多
Leaf variegation,the mosaic of colors on the leaf surface,can be developed by certain plant species without external influence.Although it may be associated with a variety of functions,the stable existence of differen...Leaf variegation,the mosaic of colors on the leaf surface,can be developed by certain plant species without external influence.Although it may be associated with a variety of functions,the stable existence of different leaf color morphs within a plant species has not been fully explained by previous studies.This study focuses on the two leaf morphs of Cypripedium forrestii,an endangered lady slipper orchid,and compares their micromorphological structure,photosynthetic potential,differentially expressed genes(DEGs),and ecological features to gain a comprehensive understanding of the underlying leaf variegation polymorphism.Our findings demonstrate that leaf variegation is not pathological and does not affect photosynthetic potential.Additionally,it significantly reduces herbivory damage.We found that the probability of herbivory and leaf area loss for variegated leaves was notably higher under drought conditions.Therefore,variegated individuals may be more adaptive under such conditions,while non-variegated ones may be more cost-effective in normal years.These results suggest that different leaf color morphs may be favored by varying environmental conditions,and leaf polymorphism may be a legacy of ancient climate and herbivore fluctuations.展开更多
基金supported by the National Natural Science Foundation of China (grant nos. 31460050 and 31760048 to Yong-Hong Zhang)
文摘Cypripedium tibeticum is a threatened orchid which efficient conservation requires knowledge of its extent and structure of genetic variation. Using two chloroplast DNA fragments(rps16 and trnL-F), we analyzed 157 individuals from 9 populations representing the species range in China. Seven haplotypes were identified. C. tibeticum had high total genetic diversity(H_T=0.80) with major contribution to this diversity made by among-population component(G_ST=0.64, Ф_ST= 0.86). However, despite high population differentiation there was no clear phylogeographic structure. The populations CY and DC made the greatest contribution to the total gene diversity as well as allelic richness. The possible mechanisms and implications of these findings for conservation of the species are discussed.
文摘A new phenanthraquinone, cypripediquinone A, was isolated from the rhizome of Cypripedium macranthum ( Orchidaceae ) and its structure was established as 2,4,7-trimethoxy-9, 10- phenanthraquinone mainly by spectroscopic analysis.
基金supported by:Science&Technology Basic Resources Investigation Program of China for Survey and Germplasm Conservation of PSESP in Southwest China(2017FY100100)the Second Tibetan Plateau Scientific Expedition and Research Program(2019QZKK0502)+1 种基金the PSESP project of Yunnan Forestry and Grassland Bureau(2021SJ14X-09)and the project“Collection and Conservation of Plant Species with Extremely Small Populations of Polystichum glaciale and Cypripedium forrestii in Lijiang”(2021SJ14X-11).
文摘Leaf variegation,the mosaic of colors on the leaf surface,can be developed by certain plant species without external influence.Although it may be associated with a variety of functions,the stable existence of different leaf color morphs within a plant species has not been fully explained by previous studies.This study focuses on the two leaf morphs of Cypripedium forrestii,an endangered lady slipper orchid,and compares their micromorphological structure,photosynthetic potential,differentially expressed genes(DEGs),and ecological features to gain a comprehensive understanding of the underlying leaf variegation polymorphism.Our findings demonstrate that leaf variegation is not pathological and does not affect photosynthetic potential.Additionally,it significantly reduces herbivory damage.We found that the probability of herbivory and leaf area loss for variegated leaves was notably higher under drought conditions.Therefore,variegated individuals may be more adaptive under such conditions,while non-variegated ones may be more cost-effective in normal years.These results suggest that different leaf color morphs may be favored by varying environmental conditions,and leaf polymorphism may be a legacy of ancient climate and herbivore fluctuations.
