A new species of the subfamily Labeoninae, Garra nujiangensis Chen and Yang, sp. nov. is recognized from a secondary branch of Nujiang River （upper Salween） in Zhenkang county, Yunnan province, China. It can be dist...A new species of the subfamily Labeoninae, Garra nujiangensis Chen and Yang, sp. nov. is recognized from a secondary branch of Nujiang River （upper Salween） in Zhenkang county, Yunnan province, China. It can be distinguished from other beardless Garra species by the following combination of characters： 12 - 14 circumpedunele scales; 48 - 50 lateral- line scales; a pair of rudimentary rostral barbels present in little individuals; 8 - 9 branched dorsal-fin rays; 5 - 6 scales between dorsal-fin origin and lateral line and 3 - 4 scales between pelvic-fin origin and lateral line; caudal peduncle relatively stouter; length between snout tip to posterior margin of eye longer than body depth at anal fin origin; head length longer than body depth at dorsal fin origin; prepelvic length 50.0% - 53.7% of standard length; pre-anus length 58.9% - 63.9% of standard length; body width 16.7% - 19.6% of standard length; body depth at dorsal fin 19.2% - 22.6% of standard length. The present study also verified that the presence of tiny barbells is a juvenile character in some beardless Garra species, such as G. nujiangensis and G. alticorpora.展开更多
Cyprinid herpesvirus II （CyHV-2） infection is identi- fied in cultured gibel carp, Carassius auratus gibelio, with high mortality in China in recent years. Histological pathology includes acute hepatocellular necros...Cyprinid herpesvirus II （CyHV-2） infection is identi- fied in cultured gibel carp, Carassius auratus gibelio, with high mortality in China in recent years. Histological pathology includes acute hepatocellular necrosis, splenic necrosis, kidney necrosis, hyperplasia of the secondary lamellae with focal necrosis. Acute necrotic myocarditis and granulocytes are prominent within the cardiac lumen in infected fish. In addition, necrosis is observed in the submucosa and mucosa epithelium of intestinal tract. Edemas are observed in renal glomerulus, submucosa and mucosa epithe- lium of intestinal tract, myocardial cells and neurons. Transmis- sion electron microscopy indicates the cytoplasmic inclusions in splenocytes, glomerulus cells and hematopoietic tissue cells of kidney, epithelial cells of gills and brain cells. The histopathology and ultrastructural pathology in CyHV-2 infected gibel carp are characterized with extensive necrosis and cytoplasmic inclusions in spleen, kidney, gill and brain, which suggests that CyHV-2 may mainly infect the spleen, kidney, gill and brain of fish.展开更多
Cyprinid herpesvirus 3(CyHV-3)and Cyprinid herpesvirus 2(CyHV-2)both belong to Alloherpesviridae family and bring great harm to koi carp,goldfish and crucian carp industry worldwide.In order to prepare a cross monoclo...Cyprinid herpesvirus 3(CyHV-3)and Cyprinid herpesvirus 2(CyHV-2)both belong to Alloherpesviridae family and bring great harm to koi carp,goldfish and crucian carp industry worldwide.In order to prepare a cross monoclonal antibody(MAb)that can simultaneously detect CyHV-3 and CyHV-2,mice were immunized with purified CyHV-3,and their spleens were taken for cell fusion with SP2/0 cells.Hybridoma cells were detected with purified CyHV-3 and CyHV-2.Western blotting was performed on the screened positive cell lines,and then the supernatant of the screened positive cell lines was used to detect common carp brain(CCB)cells infected with CyHV-3 and caudal fin of Carassius auratus gibelio(GiCF)cells infected with CyHV-2.The results showed that two strains of MAbs 3E11 and 4E4 could recognize CyHV-3 and CyHV-2 by dot blot.MAbs 3E11 and 4E4 could detect viruses in CCB cells infected with CyHV-3 and GiCF cells infected with CyHV-2.In this study,two strains of CyHV-3 and CyHV-2 cross MAbs were prepared to detect CyHV-3 and CyHV-2 simultaneously,providing more technical support for the establishment of detection methods for CyHV-3 and CyHV-2.展开更多
Crucian carp(Carassius auratus gibelio),an extensively cultivated freshwater fish,was one of the model species for the study of fish immunology.Polyclonal antibodies were advantageous molecular tools for studying tele...Crucian carp(Carassius auratus gibelio),an extensively cultivated freshwater fish,was one of the model species for the study of fish immunology.Polyclonal antibodies were advantageous molecular tools for studying teleost immune system.Specifically,polyclonal antibodies reacting with immunoglobulins(Ig)were used successfully in studies of the teleost fishes.In the present study,we produced polyclonal antibody against CH2 domains of crucian carp IgM,and measured the in vivo dynamics of IgM mRNA caused by CyHV-2 infection.The recombinant protein IgM with relative molecular weight about 53 KD was correctly expressed in prokaryotic cells.The specificity of the polyclonal antibody was evaluated by Western blotting and results revealed that the antibody not only specifically recognized crucian carp serum but also cross-reacted with grass carp serum.Quantitative RT-PCR analysis demonstrated the expression of IgM mRNA changed significantly after CyHV-2 infection.The expression of IgM in the kidney increased and reached a maximum at 6 h post-infection(hpi),while dropped to a low level at 5 days post-infection(dpi).In conclusion,the expression of IgM was significantly upregulated in the kidney of crucian carp infected with CyHV-2,indicating that IgM played a potential role in systemic immunity against viral infection.Polyclonal antibody against crucian carp IgM had certain clinical relevance,which might provide insight into the early stage of virus infection and prevention of the disease.展开更多
Neomycin belongs to the family of 2-deoxystreptamine-containing aminoglycoside antibiotics.It is widely used for bacterial infections,targeting most gram-negative and some gram-positive bacteria.Neomycin has also been...Neomycin belongs to the family of 2-deoxystreptamine-containing aminoglycoside antibiotics.It is widely used for bacterial infections,targeting most gram-negative and some gram-positive bacteria.Neomycin has also been reported to show antiviral activity.Here,we evaluated the toxicity of neomycin sulfate,and investigated its effect on non-specific immunity and viral infection in crucian carp(Carassius auratus gibelio).The safe concentration of neomycin sulfate for crucian carp was determined to be 102.9 mg/kg in vivo.In oxidative damage assays,neomycin sulfate increased superoxide dismutase and catalase activity and decreased malondialdehyde in the liver of crucian carp.In non-specific blood immune assays,the white blood cell count and complement 3 content significantly increased after neomycin sulfate treatment,while no significant changes were observed in antibacterial or lysozyme activity.In a challenge test,neomycin sulfate protected the crucian carp from cyprinid herpesvirus 2(CyHV-2)infection and inhibited CyHV-2 replication.In cytotoxicity assays,low concentrations of neomycin sulfate had no cytotoxicity on cells from the fins of crucian carp.The results of the present study indicate that oral administration of neomycin sulfate reduced oxidative damage,enhanced immunity and provided protection against CyHV-2 in crucian carp.展开更多
文摘A new species of the subfamily Labeoninae, Garra nujiangensis Chen and Yang, sp. nov. is recognized from a secondary branch of Nujiang River （upper Salween） in Zhenkang county, Yunnan province, China. It can be distinguished from other beardless Garra species by the following combination of characters： 12 - 14 circumpedunele scales; 48 - 50 lateral- line scales; a pair of rudimentary rostral barbels present in little individuals; 8 - 9 branched dorsal-fin rays; 5 - 6 scales between dorsal-fin origin and lateral line and 3 - 4 scales between pelvic-fin origin and lateral line; caudal peduncle relatively stouter; length between snout tip to posterior margin of eye longer than body depth at anal fin origin; head length longer than body depth at dorsal fin origin; prepelvic length 50.0% - 53.7% of standard length; pre-anus length 58.9% - 63.9% of standard length; body width 16.7% - 19.6% of standard length; body depth at dorsal fin 19.2% - 22.6% of standard length. The present study also verified that the presence of tiny barbells is a juvenile character in some beardless Garra species, such as G. nujiangensis and G. alticorpora.
基金Supported by the Earmarked Fund for China Agriculture Research System(CARS-46-11)the Special Scientific Research Funds for Central Non-profit Institute,Chinese Academy of Fishery Sciences(2014A06XY07)the Wuhan Science and Technology Plan Grant,Hubei Province(2014020202010138)
文摘Cyprinid herpesvirus II （CyHV-2） infection is identi- fied in cultured gibel carp, Carassius auratus gibelio, with high mortality in China in recent years. Histological pathology includes acute hepatocellular necrosis, splenic necrosis, kidney necrosis, hyperplasia of the secondary lamellae with focal necrosis. Acute necrotic myocarditis and granulocytes are prominent within the cardiac lumen in infected fish. In addition, necrosis is observed in the submucosa and mucosa epithelium of intestinal tract. Edemas are observed in renal glomerulus, submucosa and mucosa epithe- lium of intestinal tract, myocardial cells and neurons. Transmis- sion electron microscopy indicates the cytoplasmic inclusions in splenocytes, glomerulus cells and hematopoietic tissue cells of kidney, epithelial cells of gills and brain cells. The histopathology and ultrastructural pathology in CyHV-2 infected gibel carp are characterized with extensive necrosis and cytoplasmic inclusions in spleen, kidney, gill and brain, which suggests that CyHV-2 may mainly infect the spleen, kidney, gill and brain of fish.
基金supported by the National Key R&D Program of China(2019YFD0900101)Development and Demonstration Application of Rapid Diagnosis Technology for Major Aquatic Viral Diseases in Jiangsu Province(BE2021369).
文摘Cyprinid herpesvirus 3(CyHV-3)and Cyprinid herpesvirus 2(CyHV-2)both belong to Alloherpesviridae family and bring great harm to koi carp,goldfish and crucian carp industry worldwide.In order to prepare a cross monoclonal antibody(MAb)that can simultaneously detect CyHV-3 and CyHV-2,mice were immunized with purified CyHV-3,and their spleens were taken for cell fusion with SP2/0 cells.Hybridoma cells were detected with purified CyHV-3 and CyHV-2.Western blotting was performed on the screened positive cell lines,and then the supernatant of the screened positive cell lines was used to detect common carp brain(CCB)cells infected with CyHV-3 and caudal fin of Carassius auratus gibelio(GiCF)cells infected with CyHV-2.The results showed that two strains of MAbs 3E11 and 4E4 could recognize CyHV-3 and CyHV-2 by dot blot.MAbs 3E11 and 4E4 could detect viruses in CCB cells infected with CyHV-3 and GiCF cells infected with CyHV-2.In this study,two strains of CyHV-3 and CyHV-2 cross MAbs were prepared to detect CyHV-3 and CyHV-2 simultaneously,providing more technical support for the establishment of detection methods for CyHV-3 and CyHV-2.
基金supported by the General Fund Project of China Shanghai Natural Science Foundation(Program No.22ZR1427200).
文摘Crucian carp(Carassius auratus gibelio),an extensively cultivated freshwater fish,was one of the model species for the study of fish immunology.Polyclonal antibodies were advantageous molecular tools for studying teleost immune system.Specifically,polyclonal antibodies reacting with immunoglobulins(Ig)were used successfully in studies of the teleost fishes.In the present study,we produced polyclonal antibody against CH2 domains of crucian carp IgM,and measured the in vivo dynamics of IgM mRNA caused by CyHV-2 infection.The recombinant protein IgM with relative molecular weight about 53 KD was correctly expressed in prokaryotic cells.The specificity of the polyclonal antibody was evaluated by Western blotting and results revealed that the antibody not only specifically recognized crucian carp serum but also cross-reacted with grass carp serum.Quantitative RT-PCR analysis demonstrated the expression of IgM mRNA changed significantly after CyHV-2 infection.The expression of IgM in the kidney increased and reached a maximum at 6 h post-infection(hpi),while dropped to a low level at 5 days post-infection(dpi).In conclusion,the expression of IgM was significantly upregulated in the kidney of crucian carp infected with CyHV-2,indicating that IgM played a potential role in systemic immunity against viral infection.Polyclonal antibody against crucian carp IgM had certain clinical relevance,which might provide insight into the early stage of virus infection and prevention of the disease.
基金This work was supported by the Earmarked Fund for China Agriculture Research System(grant number CARS-45-19).The authors report no conflicts of interest in this paper.
文摘Neomycin belongs to the family of 2-deoxystreptamine-containing aminoglycoside antibiotics.It is widely used for bacterial infections,targeting most gram-negative and some gram-positive bacteria.Neomycin has also been reported to show antiviral activity.Here,we evaluated the toxicity of neomycin sulfate,and investigated its effect on non-specific immunity and viral infection in crucian carp(Carassius auratus gibelio).The safe concentration of neomycin sulfate for crucian carp was determined to be 102.9 mg/kg in vivo.In oxidative damage assays,neomycin sulfate increased superoxide dismutase and catalase activity and decreased malondialdehyde in the liver of crucian carp.In non-specific blood immune assays,the white blood cell count and complement 3 content significantly increased after neomycin sulfate treatment,while no significant changes were observed in antibacterial or lysozyme activity.In a challenge test,neomycin sulfate protected the crucian carp from cyprinid herpesvirus 2(CyHV-2)infection and inhibited CyHV-2 replication.In cytotoxicity assays,low concentrations of neomycin sulfate had no cytotoxicity on cells from the fins of crucian carp.The results of the present study indicate that oral administration of neomycin sulfate reduced oxidative damage,enhanced immunity and provided protection against CyHV-2 in crucian carp.
