Objective:To study the expression and significance of cyclinD2 and Bcl-2 in diffuse large B-cell lymphoma.Methods:This project used immunohistochemical methods to detect the expression of cyclinD2 and Bcl-2 in 120 cas...Objective:To study the expression and significance of cyclinD2 and Bcl-2 in diffuse large B-cell lymphoma.Methods:This project used immunohistochemical methods to detect the expression of cyclinD2 and Bcl-2 in 120 cases of diffuse large B-cell lymphoma and 80 cases of reactive hyperplasia of lymphoid tissue.The materials were collected from the hospital from January 2018 to 2020.In March 2015,120 patients had lymphoma tissue removed during the month of surgery.The postoperative pathological diagnosis was DLBCL.Another 80 cases of lymphoid hyperplasia(RLH)tissues were selected as controls.Results:CyclinD2 and BCL-2 expression were not statistically different in patients with diffuse large B-cell lymphoma in different ages,genders,locations,tissue types,and degree of differentiation;but statistically significant in different Ann Arbor stages,immunotypes,IPI index and first treatment efficacy.Conclusion:This research not only has important theoretical value,but also important economic value and social significance.展开更多
Objective:To study the expression and significance of cell cycle proteins CyclinD2,mPGES-1,Bcl2 in diffuse large B-cell lymphoma.Methods:Choose lymphoma and sexually hyper-plastic lymphoid tissues as control.Immunohis...Objective:To study the expression and significance of cell cycle proteins CyclinD2,mPGES-1,Bcl2 in diffuse large B-cell lymphoma.Methods:Choose lymphoma and sexually hyper-plastic lymphoid tissues as control.Immunohistoc-hemical methods were used to detect the expression of CyclinD2,mPGES-1,and Bcl2,and to compare the positive expression rates of CyclinD2,MPGES-1 and Bcl2 in diffuse large B-cell lymphoma and reactive proliferative lymphoid tissues to compare their diffusion formation.B-cell lymphoma was analyzed for its clinicopathological features.Results:The positive expression rate of CyclinD2,mPGES-1 and Bcl2 in diffuse large B-cell lymphoma is higher than that in reactive proliferative lymphoid tissue,and the difference between the two is statistically significant.There was no statistical difference in CyclinD2,mPGES-1 and Bcl2 in diffuse large B-cell lymphoma between patients according to the age,sex,location,tissue type and degree of differentiation.Conclusion:CyclinD2,mPGES-1 and Bcl2 are highly expressed in patients with diffuse large B-cell lymphoma,and can be used as reference indicators for evaluating the malignant degree and efficacy of dysplasia.展开更多
Background:This research aimed to examine the impact of co-transplantation of ethyl stearate(PubChem CID:8122)and neural stem cells(NSCs)on endogenous NSCs in Parkinson's disease(PD)model rats,and also determine t...Background:This research aimed to examine the impact of co-transplantation of ethyl stearate(PubChem CID:8122)and neural stem cells(NSCs)on endogenous NSCs in Parkinson's disease(PD)model rats,and also determine the impact of ethyl stearate on quiescent neural stem cells(qNSCs)and explore its possible mechanism.Methods:The induction of PD rats was achieved through the injection of 6-hydroxydopamine(6-OHDA)into the right striatum(STR)and substantia nigra(SN).Transplant treatment was then followed for 6 weeks in the right STR.Apomorphine(APO)-induced rotation behavior and the pole climbing test were used to assess the effects of transplant treatment in each group.Western blotting(WB)and immunofluorescence staining were used to observe changes in endogenous NSCs.The effect of ethyl stearate on qNSCs was examined in vitro,with detection of associated indicators achieved through WB,immunofluorescence,flow cytometry and qRT-PCR.Results:Co-transplantation of NSCs with ethyl stearate significantly ameliorated motor deficits in PD rats and upregulated the expression of tyrosine hydroxylase(TH),Sox2,and Nestin.We established an in vitro system for NSC quiescence and activation.In qNSCs,ethyl stearate treatment increased Ki67 positivity and reduced the proportion of cells in the G0-G1 phase of the cell cycle,whereas withdrawal of ethyl stearate exerted the opposite effects.Ethyl stearate also downregulated EphB2 protein levels and upregulated p-AKT and CyclinD1 in qNSCs;these effects were reversed upon its removal.Conclusion:These findings imply that co-transplantation of NSCs and ethyl stearate may help ameliorate motor impairment in PD rats by activating endogenous NSCs and promoting their development into dopaminergic neurons.Ethyl stearate may regulate the entry and exit of NSCs from quiescence through the EphB2/AKT/CyclinD1 pathway.展开更多
文摘Objective:To study the expression and significance of cyclinD2 and Bcl-2 in diffuse large B-cell lymphoma.Methods:This project used immunohistochemical methods to detect the expression of cyclinD2 and Bcl-2 in 120 cases of diffuse large B-cell lymphoma and 80 cases of reactive hyperplasia of lymphoid tissue.The materials were collected from the hospital from January 2018 to 2020.In March 2015,120 patients had lymphoma tissue removed during the month of surgery.The postoperative pathological diagnosis was DLBCL.Another 80 cases of lymphoid hyperplasia(RLH)tissues were selected as controls.Results:CyclinD2 and BCL-2 expression were not statistically different in patients with diffuse large B-cell lymphoma in different ages,genders,locations,tissue types,and degree of differentiation;but statistically significant in different Ann Arbor stages,immunotypes,IPI index and first treatment efficacy.Conclusion:This research not only has important theoretical value,but also important economic value and social significance.
基金Qiushuang Ma and Peng Lin both are the first authors and contributed equally to this work.*Jinku Zhang and Jirui Sun are the corresponding authors and contributed equally to this work。
文摘Objective:To study the expression and significance of cell cycle proteins CyclinD2,mPGES-1,Bcl2 in diffuse large B-cell lymphoma.Methods:Choose lymphoma and sexually hyper-plastic lymphoid tissues as control.Immunohistoc-hemical methods were used to detect the expression of CyclinD2,mPGES-1,and Bcl2,and to compare the positive expression rates of CyclinD2,MPGES-1 and Bcl2 in diffuse large B-cell lymphoma and reactive proliferative lymphoid tissues to compare their diffusion formation.B-cell lymphoma was analyzed for its clinicopathological features.Results:The positive expression rate of CyclinD2,mPGES-1 and Bcl2 in diffuse large B-cell lymphoma is higher than that in reactive proliferative lymphoid tissue,and the difference between the two is statistically significant.There was no statistical difference in CyclinD2,mPGES-1 and Bcl2 in diffuse large B-cell lymphoma between patients according to the age,sex,location,tissue type and degree of differentiation.Conclusion:CyclinD2,mPGES-1 and Bcl2 are highly expressed in patients with diffuse large B-cell lymphoma,and can be used as reference indicators for evaluating the malignant degree and efficacy of dysplasia.
基金The Medical Scientific Research Foundation of Guangdong Province,Grant/Award Number:B2020002National Natural Science Foundation of China,Grant/Award Number:82474266Science and Technology Planning Project of Guangdong Province,Grant/Award Number:2016A020226017。
文摘Background:This research aimed to examine the impact of co-transplantation of ethyl stearate(PubChem CID:8122)and neural stem cells(NSCs)on endogenous NSCs in Parkinson's disease(PD)model rats,and also determine the impact of ethyl stearate on quiescent neural stem cells(qNSCs)and explore its possible mechanism.Methods:The induction of PD rats was achieved through the injection of 6-hydroxydopamine(6-OHDA)into the right striatum(STR)and substantia nigra(SN).Transplant treatment was then followed for 6 weeks in the right STR.Apomorphine(APO)-induced rotation behavior and the pole climbing test were used to assess the effects of transplant treatment in each group.Western blotting(WB)and immunofluorescence staining were used to observe changes in endogenous NSCs.The effect of ethyl stearate on qNSCs was examined in vitro,with detection of associated indicators achieved through WB,immunofluorescence,flow cytometry and qRT-PCR.Results:Co-transplantation of NSCs with ethyl stearate significantly ameliorated motor deficits in PD rats and upregulated the expression of tyrosine hydroxylase(TH),Sox2,and Nestin.We established an in vitro system for NSC quiescence and activation.In qNSCs,ethyl stearate treatment increased Ki67 positivity and reduced the proportion of cells in the G0-G1 phase of the cell cycle,whereas withdrawal of ethyl stearate exerted the opposite effects.Ethyl stearate also downregulated EphB2 protein levels and upregulated p-AKT and CyclinD1 in qNSCs;these effects were reversed upon its removal.Conclusion:These findings imply that co-transplantation of NSCs and ethyl stearate may help ameliorate motor impairment in PD rats by activating endogenous NSCs and promoting their development into dopaminergic neurons.Ethyl stearate may regulate the entry and exit of NSCs from quiescence through the EphB2/AKT/CyclinD1 pathway.
